Rroxscaffold_1G00071220

Belongs to the plant LTP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004528
Physical Location & Seq
Forward (+)
92109443 .. 92114345
4903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071220.1

Sequence Viewer

Length: 363 bp
ATGGAGATCAAAATGGTTTCCTTCTTGGCTTTCGCCTTGGCGATTATGGCCATTGTTCTCAATGCTTCCCCAATAAATGGGGATATCACATGCCAAGCTGCTTTGGTGTGCTGCCATCCCTGTGCCACGTTCGTGATGGGTCCTGCAGAAGGAACTCCCCTCCTGCGTTGCTGTGCAGGTTTAAAGCATGTGGTTGCTGGAGCTGATACCAGTGAGAAGCGCAGGAGTCTTTGCCAATGTTTGAAAGACAAAGCTGTTGGTGCTGGCGTTAAAGTCGACCCTGAAAAACTTAAGCACGTTACTGACGCTTGTGGCGTCAAAATTCCTATTCCTATTGATCCCAATGTCGACTGCAGCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

120

Amino Acids

12.54

Weight (kDa)

7.5

Isoelectric Point (pI)

26.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 31 - 118 1.6e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 77
AccI GTMKAC 2 cut(s) 276, 348
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 315
AfiI CCNNNNNNNGG 2 cut(s) 77, 149
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 1 cut(s) 244
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 3 cut(s) 98, 203, 254
AluI AGCT 3 cut(s) 98, 203, 254
AlwI GGATC 1 cut(s) 332
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 98, 111, 354
ApoI RAATTY 1 cut(s) 321
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 140
AvaII GGWCC 1 cut(s) 140
BalI TGGCCA 1 cut(s) 50
BbvI GCAGC 2 cut(s) 85, 98
BccI CCATC 2 cut(s) 123, 130
BfmI CTRYAG 2 cut(s) 144, 352
BfrI CTTAAG 1 cut(s) 290
BfuAI ACCTGC 1 cut(s) 167
BisI GCNGC 3 cut(s) 99, 112, 355
BlsI GCNGC 3 cut(s) 100, 113, 356
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 149
Bse1I ACTGG 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 2 cut(s) 77, 149
BseNI ACTGG 1 cut(s) 210
BseXI GCAGC 2 cut(s) 85, 98
BsgI GTGCAG 1 cut(s) 195
BshFI GGCC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 77, 149
BsnI GGCC 1 cut(s) 50
Bsp143I GATC 2 cut(s) 6, 337
BspANI GGCC 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 141
BspMAI CTGCAG 2 cut(s) 148, 356
BspMI ACCTGC 1 cut(s) 167
BspPI GGATC 1 cut(s) 332
BspTI CTTAAG 1 cut(s) 290
BsrI ACTGG 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 2 cut(s) 6, 337
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 315
BstAFI CTTAAG 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 115
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 2 cut(s) 9, 340
BstMBI GATC 2 cut(s) 6, 337
BstMWI GCNNNNNNNGC 2 cut(s) 47, 260
BstNSI RCATGY 2 cut(s) 93, 191
BstSFI CTRYAG 2 cut(s) 144, 352
BstV1I GCAGC 2 cut(s) 85, 98
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 217
BveI ACCTGC 1 cut(s) 167
Cac8I GCNNGC 1 cut(s) 265
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 140
CseI GACGC 2 cut(s) 304, 314
CviAII CATG 2 cut(s) 90, 188
CviJI RGCY 5 cut(s) 29, 50, 98, 203, 254
CviKI_1 RGCY 5 cut(s) 29, 50, 98, 203, 254
DpnI GATC 2 cut(s) 8, 339
DpnII GATC 2 cut(s) 6, 337
DraI TTTAAA 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 36
Eco32I GATATC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 140
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 140
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 2 cut(s) 93, 191
FaiI YATR 3 cut(s) 47, 91, 189
FatI CATG 2 cut(s) 89, 187
FblI GTMKAC 2 cut(s) 276, 348
Fnu4HI GCNGC 3 cut(s) 99, 112, 355
FokI GGATG 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 99, 112, 355
GlaI GCGC 1 cut(s) 221
GluI GCNGC 3 cut(s) 99, 112, 355
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 1 cut(s) 50
HgaI GACGC 2 cut(s) 304, 314
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 2 cut(s) 93, 191
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 2 cut(s) 277, 349
HindII GTYRAC 2 cut(s) 277, 349
HinfI GANTC 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 277, 349
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 2 cut(s) 277, 349
HpyAV CCTTC 2 cut(s) 31, 143
HpyCH4IV ACGT 3 cut(s) 128, 297, 359
HpyCH4V TGCA 3 cut(s) 146, 176, 354
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 260
HpySE526I ACGT 3 cut(s) 128, 297, 359
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 2 cut(s) 93, 191
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 6, 337
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 9 cut(s) 133, 156, 162, 176, 183, 208, 223, 249, 294
Lsp1109I GCAGC 2 cut(s) 85, 98
MaeII ACGT 3 cut(s) 128, 297, 359
MaeIII GTNAC 1 cut(s) 298
MalI GATC 2 cut(s) 8, 339
MboI GATC 2 cut(s) 6, 337
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 1 cut(s) 321
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 1 cut(s) 170
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MseI TTAA 3 cut(s) 182, 270, 291
MslI CAYNNNNRTG 2 cut(s) 120, 131
Msp20I TGGCCA 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 47, 260
NdeII GATC 2 cut(s) 6, 337
NlaIII CATG 2 cut(s) 93, 191
NlaIV GGNNCC 1 cut(s) 141
NspI RCATGY 2 cut(s) 93, 191
OliI CACNNNNGTG 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 3 cut(s) 100, 113, 356
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Ppu21I YACGTR 1 cut(s) 360
PpuMI RGGWCCY 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 140
PspPPI RGGWCCY 1 cut(s) 140
PstI CTGCAG 2 cut(s) 148, 356
RseI CAYNNNNRTG 2 cut(s) 120, 131
SalI GTCGAC 2 cut(s) 275, 347
SaqAI TTAA 3 cut(s) 182, 270, 291
SatI GCNGC 3 cut(s) 99, 112, 355
Sau3AI GATC 2 cut(s) 6, 337
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 1 cut(s) 235
SetI ASST 7 cut(s) 100, 131, 181, 205, 256, 300, 362
SfcI CTRYAG 2 cut(s) 144, 352
SinI GGWCC 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 120, 131
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 1 cut(s) 321
StyI CCWWGG 1 cut(s) 36
TaiI ACGT 3 cut(s) 131, 300, 362
TaqI TCGA 2 cut(s) 276, 348
TasI AATT 1 cut(s) 321
Tru1I TTAA 3 cut(s) 182, 270, 291
Tru9I TTAA 3 cut(s) 182, 270, 291
TscAI CASTG 1 cut(s) 217
TseI GCWGC 3 cut(s) 98, 111, 354
TspRI CASTG 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 77
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 140
XagI CCTNNNNNAGG 1 cut(s) 147
XapI RAATTY 1 cut(s) 321
XceI RCATGY 2 cut(s) 93, 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmiI GTMKAC 2 cut(s) 276, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.