Rw5G001360

Belongs to the plant LTP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004528
Physical Location & Seq
Forward (+)
1112030 .. 1123821
11792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001360.1

Sequence Viewer

Length: 366 bp
ATGATGACTTTCACTACGCTGGTGATTATGGTCGTCGTCCTCAATGCATGTCCTGCAATTGGAGGATGCGACGAAGCCTTAGTCGAGTTGTTGCCCTGTGCCACTTTCGTGATGGGTCCTGCAGAAGGAACTCCCCCTCCTGCATGTTGTGAAGGTATGCATCATGTCATTGGTGGAGCAGATACCGCTGAGAAGCGCAGGAGTCTTTGCCAATGTTTGAAAGACACAGCTATTAAAAATGAGGTTAAAATCAAGCCTGAAAACATTAAGCATGCTGTTGATGCTTGTGGTGTTAAAATCCTTGTTCCTATTGATCCTAATATCGACTGCAGCAAGGATCAAATAAAACTTGGAGAAGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

121

Amino Acids

12.86

Weight (kDa)

4.96

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 13 - 99 6.7e-07 Probable lipid transfer
Tryp_alpha_amyl PF00234 23 - 110 1.8e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 186
AclWI GGATC 2 cut(s) 308, 345
AfiI CCNNNNNNNGG 2 cut(s) 59, 125
AgsI TTSAA 1 cut(s) 220
AleI CACNNNNGTG 1 cut(s) 107
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
AluBI AGCT 1 cut(s) 230
AluI AGCT 1 cut(s) 230
AlwI GGATC 2 cut(s) 308, 345
ApeKI GCWGC 1 cut(s) 330
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 342
BccI CCATC 1 cut(s) 106
BfmI CTRYAG 2 cut(s) 120, 328
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 117
BmsI GCATC 3 cut(s) 56, 169, 271
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 125
BseGI GGATG 1 cut(s) 71
BseLI CCNNNNNNNGG 2 cut(s) 59, 125
BseMII CTCAG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 59, 125
Bsp143I GATC 2 cut(s) 313, 337
BspACI CCGC 1 cut(s) 186
BspCNI CTCAG 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 117
BspMAI CTGCAG 2 cut(s) 124, 332
BspPI GGATC 2 cut(s) 308, 345
BssMI GATC 2 cut(s) 313, 337
BstAPI GCANNNNNTGC 1 cut(s) 53
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 79, 189
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 316, 340
BstMBI GATC 2 cut(s) 313, 337
BstMWI GCNNNNNNNGC 3 cut(s) 53, 185, 281
BstNSI RCATGY 3 cut(s) 51, 147, 275
BstSFI CTRYAG 2 cut(s) 120, 328
BstV1I GCAGC 1 cut(s) 342
BtsCI GGATG 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 273
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 116
CviAII CATG 4 cut(s) 48, 144, 164, 272
CviJI RGCY 3 cut(s) 77, 230, 256
CviKI_1 RGCY 3 cut(s) 77, 230, 256
DdeI CTNAG 2 cut(s) 79, 189
DpnI GATC 2 cut(s) 315, 339
DpnII GATC 2 cut(s) 313, 337
Eco47I GGWCC 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 116
EcoT22I ATGCAT 2 cut(s) 49, 162
FaeI CATG 4 cut(s) 51, 147, 167, 275
FaiI YATR 6 cut(s) 29, 49, 145, 158, 165, 273
FatI CATG 4 cut(s) 47, 143, 163, 271
Fnu4HI GCNGC 1 cut(s) 331
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 331
GlaI GCGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 331
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 51, 147, 167, 275
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 1 cut(s) 202
HphI GGTGA 1 cut(s) 34
Hpy188III TCNNGA 1 cut(s) 109
Hpy99I CGWCG 2 cut(s) 38, 74
HpyAV CCTTC 2 cut(s) 119, 146
HpyCH4V TGCA 6 cut(s) 47, 56, 122, 143, 160, 330
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 185, 281
HpyF3I CTNAG 2 cut(s) 79, 189
Hsp92II CATG 4 cut(s) 51, 147, 167, 275
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 313, 337
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 7 cut(s) 5, 66, 109, 132, 153, 184, 270
Lsp1109I GCAGC 1 cut(s) 342
LweI GCATC 3 cut(s) 56, 169, 271
MalI GATC 2 cut(s) 315, 339
MboI GATC 2 cut(s) 313, 337
MfeI CAATTG 1 cut(s) 57
MluCI AATT 1 cut(s) 57
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 4 cut(s) 50, 56, 147, 235
Mph1103I ATGCAT 2 cut(s) 49, 162
MseI TTAA 4 cut(s) 234, 246, 267, 294
MslI CAYNNNNRTG 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 188
MunI CAATTG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 53, 185, 281
NdeII GATC 2 cut(s) 313, 337
NlaIII CATG 4 cut(s) 51, 147, 167, 275
NlaIV GGNNCC 1 cut(s) 117
NsiI ATGCAT 2 cut(s) 49, 162
NspI RCATGY 3 cut(s) 51, 147, 275
OliI CACNNNNGTG 1 cut(s) 107
PaeI GCATGC 1 cut(s) 275
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 210
PpuMI RGGWCCY 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 1 cut(s) 116
PspPPI RGGWCCY 1 cut(s) 116
PstI CTGCAG 2 cut(s) 124, 332
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 4 cut(s) 234, 246, 267, 294
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 2 cut(s) 313, 337
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 1 cut(s) 211
SetI ASST 3 cut(s) 157, 232, 246
SfaNI GCATC 3 cut(s) 56, 169, 271
SfcI CTRYAG 2 cut(s) 120, 328
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 107
SphI GCATGC 1 cut(s) 275
Sse9I AATT 1 cut(s) 57
SsiI CCGC 1 cut(s) 186
TaqI TCGA 2 cut(s) 84, 324
TasI AATT 1 cut(s) 57
Tru1I TTAA 4 cut(s) 234, 246, 267, 294
Tru9I TTAA 4 cut(s) 234, 246, 267, 294
TseI GCWGC 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 116
XagI CCTNNNNNAGG 1 cut(s) 123
XceI RCATGY 3 cut(s) 51, 147, 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
Zsp2I ATGCAT 2 cut(s) 49, 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.