Rroxscaffold_1G00074560

Belongs to the plant LTP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004528
Physical Location & Seq
Reverse (-)
95508459 .. 95508797
339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074560.1

Sequence Viewer

Length: 339 bp
ATGATGACTTTCGCTGGGTTGGTGATTATGGTCGTCGTCCTCAATGCATGTCCTGCAATTGGAGGGTGCGACGAAGCCTTAGTCGAGTTGTTGCCCTGTGCCACGTTCGTGTTGGGCCCTGGCATAGGGGAGCCCCCTTCTCTGTGCTGTGCAGGTTTAAAGCATGTGGTTGCTGGAGCTGATACCAGTGAGAAGCGCAGGAGTCTTTGCCAATGTTTGAAAGACAAAGCTGTTGGTGCTGGCGTTAAAGTCGACCCTGAAAAACTTAAGCACGTTACTGACGCTTGTGGCGTCAAAATTCCTATTCCTATTGATCCCAATGTCGACTGCAGCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

112

Amino Acids

11.5

Weight (kDa)

5.56

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 14 - 101 3e-08 Probable lipid transfer
Tryp_alpha_amyl PF00234 23 - 110 4.1e-11 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 252, 324
AclWI GGATC 1 cut(s) 308
AcsI RAATTY 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 291
AfiI CCNNNNNNNGG 2 cut(s) 59, 125
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 1 cut(s) 118
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 2 cut(s) 179, 230
AluI AGCT 2 cut(s) 179, 230
AlwI GGATC 1 cut(s) 308
AoxI GGCC 1 cut(s) 115
ApaI GGGCCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 330
ApoI RAATTY 1 cut(s) 297
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 2 cut(s) 115, 116
AsuHPI GGTGA 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 119
BanII GRGCYC 2 cut(s) 119, 135
BciT130I CCWGG 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 328
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 115, 116
BmiI GGNNCC 2 cut(s) 117, 132
BmrFI CCNGG 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 125
Bse1I ACTGG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 59, 125
BseNI ACTGG 1 cut(s) 186
BseSI GKGCMC 1 cut(s) 119
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 59, 125
BsnI GGCC 1 cut(s) 117
Bsp120I GGGCCC 1 cut(s) 115
Bsp1286I GDGCHC 2 cut(s) 119, 135
Bsp143I GATC 1 cut(s) 313
BspANI GGCC 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 117, 132
BspMAI CTGCAG 1 cut(s) 332
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 266
BsrI ACTGG 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 1 cut(s) 313
BssNI GRCGYC 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 291
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 79
BstENI CCTNNNNNAGG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 1 cut(s) 316
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 2 cut(s) 53, 236
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 2 cut(s) 51, 167
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 119
BsuRI GGCC 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 193
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 2 cut(s) 115, 116
CseI GACGC 2 cut(s) 280, 290
CviAII CATG 2 cut(s) 48, 164
CviJI RGCY 5 cut(s) 77, 117, 133, 179, 230
CviKI_1 RGCY 5 cut(s) 77, 117, 133, 179, 230
DdeI CTNAG 1 cut(s) 79
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
DraI TTTAAA 1 cut(s) 159
Eco24I GRGCYC 2 cut(s) 119, 135
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 119, 135
FaeI CATG 2 cut(s) 51, 167
FaiI YATR 4 cut(s) 29, 49, 125, 165
FatI CATG 2 cut(s) 47, 163
FblI GTMKAC 2 cut(s) 252, 324
Fnu4HI GCNGC 1 cut(s) 331
FriOI GRGCYC 2 cut(s) 119, 135
Fsp4HI GCNGC 1 cut(s) 331
GlaI GCGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 331
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 2 cut(s) 280, 290
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 2 cut(s) 51, 167
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 2 cut(s) 253, 325
HindII GTYRAC 2 cut(s) 253, 325
HinfI GANTC 1 cut(s) 202
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 253, 325
Hpy8I GTNNAC 2 cut(s) 253, 325
Hpy99I CGWCG 2 cut(s) 38, 74
HpyAV CCTTC 1 cut(s) 147
HpyCH4IV ACGT 3 cut(s) 104, 273, 335
HpyCH4V TGCA 4 cut(s) 47, 56, 152, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 236
HpyF3I CTNAG 1 cut(s) 79
HpySE526I ACGT 3 cut(s) 104, 273, 335
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 2 cut(s) 51, 167
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 313
LmnI GCTCC 2 cut(s) 130, 176
MaeII ACGT 3 cut(s) 104, 273, 335
MaeIII GTNAC 1 cut(s) 274
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MfeI CAATTG 1 cut(s) 57
MhlI GDGCHC 2 cut(s) 119, 135
MluCI AATT 2 cut(s) 57, 297
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 2 cut(s) 50, 56
Mph1103I ATGCAT 1 cut(s) 49
MseI TTAA 3 cut(s) 158, 246, 267
MslI CAYNNNNRTG 1 cut(s) 107
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 120
MunI CAATTG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 2 cut(s) 53, 236
NdeII GATC 1 cut(s) 313
NlaIII CATG 2 cut(s) 51, 167
NlaIV GGNNCC 2 cut(s) 117, 132
NsiI ATGCAT 1 cut(s) 49
NspI RCATGY 2 cut(s) 51, 167
OliI CACNNNNGTG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 210
Ppu21I YACGTR 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 117, 132
PspOMI GGGCCC 1 cut(s) 115
PspPI GGNCC 2 cut(s) 115, 116
PstI CTGCAG 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 107
SalI GTCGAC 2 cut(s) 251, 323
SaqAI TTAA 3 cut(s) 158, 246, 267
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 1 cut(s) 313
Sau96I GGNCC 2 cut(s) 115, 116
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 2 cut(s) 119, 135
SetI ASST 6 cut(s) 107, 157, 181, 232, 276, 338
SfcI CTRYAG 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 2 cut(s) 57, 297
StyD4I CCNGG 1 cut(s) 118
TaiI ACGT 3 cut(s) 107, 276, 338
TaqI TCGA 3 cut(s) 84, 252, 324
TasI AATT 2 cut(s) 57, 297
Tru1I TTAA 3 cut(s) 158, 246, 267
Tru9I TTAA 3 cut(s) 158, 246, 267
TscAI CASTG 1 cut(s) 193
TseI GCWGC 1 cut(s) 330
TspRI CASTG 1 cut(s) 193
Vha464I CTTAAG 1 cut(s) 266
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 1 cut(s) 297
XceI RCATGY 2 cut(s) 51, 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 2 cut(s) 252, 324
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.