Rroxscaffold_2G00126320

homoserine dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014814
Physical Location & Seq
Reverse (-)
61537021 .. 61539550
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126320.1

Sequence Viewer

Length: 780 bp
ATGTTGTCGGAGACTATTGGTACTTCTTTTTGGTGGAAAGAAAAGCATCTTGCTGGTGAAAATCTTGTCTGCCACACTCACCTAAAGGAGGAGTGGAGCAACACCGCAAATCTTGTGGAGGTTGAGATTGCTCGCGAGTTCGAGACTATTCCACTGCTCACCCAAGCGATTGATTTGGGATGTTGCATTGTCATGGCAAACAAGAAGCCTCTTACTTCAACAATGGAGGATTATGACAAACTAATTTTACACCCACGTCACATACGTCATGAGTCAACTATTTGGCTTCTCGGTTGGCGTATTAATTTGGATGACGTTAAGATTGAGAGCTTGTATCCTAAAGAAATGGCACCCGGTGTAATGTCCGTGGAAGAATTTCGGGAACCTGGCTACTATTGCTTGATAAAGATATCAAAGAAAGAGTCGTCACCGGCTTTTCAAGAGAGGAAGAGCCACGAAGTCGAAGCTGAATTTGAGAAACTTTTAGGAGGAGCTCATGTTAAATTTGCAATGGTAGAATTGTCAAAGTCGAAAGAGGGGATGAATTGGAGACAAGAAAGTTCTCGTAGCTACCGTATGGCCGCCATTTCAAAATGGTTTTCATTGGGTCTACCATTTTTGCTTTACAACAGTGTCGCTTGGAAGAAGGGTGCTTCTTCTTGGAAGTTTTACGGCATGGCAGTGTCAATGAGTCTTCAAGACCCAAGTGGGGTGTTGGGATTGAGGATTGTGAATGTGTTTCCTCGTGATTCCCGTGAGTGGAATGACGTCTCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homoserine_dh NAD_binding_3

Protein Analysis

259

Amino Acids

29.81

Weight (kDa)

6.47

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 771
AccB1I GGYRCC 1 cut(s) 349
AccI GTMKAC 1 cut(s) 610
AccII CGCG 1 cut(s) 135
AciI CCGC 2 cut(s) 105, 582
AcoI YGGCCR 1 cut(s) 579
AcsI RAATTY 3 cut(s) 374, 470, 503
AcyI GRCGYC 1 cut(s) 768
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 3 cut(s) 88, 709, 759
AgsI TTSAA 4 cut(s) 219, 440, 591, 698
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 4 cut(s) 330, 467, 494, 570
AluI AGCT 4 cut(s) 330, 467, 494, 570
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 4 cut(s) 5, 137, 544, 775
AoxI GGCC 1 cut(s) 579
ApoI RAATTY 3 cut(s) 374, 470, 503
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AseI ATTAAT 1 cut(s) 303
Asp700I GAANNNNTTC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 4 cut(s) 68, 71, 151, 420
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 496
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 1 cut(s) 387
BciVI GTATCC 1 cut(s) 345
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 4 cut(s) 5, 137, 544, 775
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 1 cut(s) 582
BlsI GCNGC 1 cut(s) 583
Bme1390I CCNGG 2 cut(s) 354, 387
BmgBI CACGTC 1 cut(s) 257
BmiI GGNNCC 2 cut(s) 351, 384
BmrFI CCNGG 2 cut(s) 354, 387
BmsI GCATC 1 cut(s) 55
BpiI GAAGAC 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 768
BsaJI CCNNGG 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 709, 759
Bse118I RCCGGY 1 cut(s) 430
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 3 cut(s) 185, 316, 546
BseLI CCNNNNNNNGG 3 cut(s) 88, 709, 759
BseMI GCAATG 1 cut(s) 516
BseRI GAGGAG 2 cut(s) 104, 504
Bsh1236I CGCG 1 cut(s) 135
BshFI GGCC 1 cut(s) 581
BshNI GGYRCC 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 2 cut(s) 354, 431
BslI CCNNNNNNNGG 3 cut(s) 88, 709, 759
BsmAI GTCTC 4 cut(s) 5, 137, 544, 775
BsmBI CGTCTC 1 cut(s) 775
BsnI GGCC 1 cut(s) 581
Bsp1286I GDGCHC 1 cut(s) 496
Bsp68I TCGCGA 1 cut(s) 135
BspACI CCGC 2 cut(s) 105, 582
BspANI GGCC 1 cut(s) 581
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 2 cut(s) 351, 384
BspQI GCTCTTC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 430
BssECI CCNNGG 1 cut(s) 366
BssNI GRCGYC 1 cut(s) 768
BssSI CACGAG 1 cut(s) 744
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 575, 632
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 133
BstDSI CCRYGG 1 cut(s) 366
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 3 cut(s) 185, 316, 546
BstFNI CGCG 1 cut(s) 135
BstMAI GTCTC 4 cut(s) 5, 137, 544, 775
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 352, 385
BstUI CGCG 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 686
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 1 cut(s) 581
BtgI CCRYGG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 3 cut(s) 185, 316, 546
BtsI GCAGTG 2 cut(s) 152, 687
BtsIMutI CAGTG 3 cut(s) 152, 637, 687
BtuMI TCGCGA 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 133
CciI TCATGA 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 430
Csp6I GTAC 1 cut(s) 21
CspCI CAANNNNNGTGG 2 cut(s) 96, 131
CviAII CATG 4 cut(s) 193, 269, 497, 676
CviQI GTAC 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 356
EaeI YGGCCR 1 cut(s) 579
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 496
Eco32I GATATC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 775
FaeI CATG 4 cut(s) 196, 272, 500, 679
FaiI YATR 7 cut(s) 194, 234, 263, 270, 498, 578, 677
FatI CATG 4 cut(s) 192, 268, 496, 675
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 1 cut(s) 582
FokI GGATG 3 cut(s) 192, 323, 553
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 1 cut(s) 582
GluI GCNGC 1 cut(s) 582
HaeIII GGCC 1 cut(s) 581
HapII CCGG 2 cut(s) 354, 431
Hin1I GRCGYC 1 cut(s) 768
Hin1II CATG 4 cut(s) 196, 272, 500, 679
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 4 cut(s) 272, 422, 691, 749
HpaII CCGG 2 cut(s) 354, 431
HphI GGTGA 4 cut(s) 68, 71, 151, 420
Hpy166II GTNNAC 2 cut(s) 276, 611
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 7 cut(s) 134, 142, 269, 380, 440, 698, 746
Hpy8I GTNNAC 2 cut(s) 276, 611
HpyAV CCTTC 1 cut(s) 640
HpyCH4III ACNGT 2 cut(s) 575, 632
HpyCH4IV ACGT 4 cut(s) 256, 265, 315, 768
HpyCH4V TGCA 2 cut(s) 186, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpySE526I ACGT 4 cut(s) 256, 265, 315, 768
Hsp92I GRCGYC 1 cut(s) 768
Hsp92II CATG 4 cut(s) 196, 272, 500, 679
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 2 cut(s) 96, 491
LpnPI CCDG 5 cut(s) 39, 367, 372, 399, 444
LweI GCATC 1 cut(s) 55
MaeII ACGT 4 cut(s) 256, 265, 315, 768
MaeIII GTNAC 2 cut(s) 257, 426
MboII GAAGA 5 cut(s) 383, 460, 648, 655, 686
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 7 cut(s) 243, 304, 374, 470, 503, 518, 544
MlyI GAGTC 3 cut(s) 281, 431, 700
MnlI CCTC 9 cut(s) 82, 112, 219, 220, 438, 482, 529, 717, 753
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 3 cut(s) 303, 318, 501
MslI CAYNNNNRTG 2 cut(s) 191, 680
MspI CCGG 2 cut(s) 354, 431
MspR9I CCNGG 2 cut(s) 354, 387
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 396
NciI CCSGG 1 cut(s) 354
NlaIII CATG 4 cut(s) 196, 272, 500, 679
NlaIV GGNNCC 2 cut(s) 351, 384
NmuCI GTSAC 2 cut(s) 257, 426
NruI TCGCGA 1 cut(s) 135
PagI TCATGA 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 3 cut(s) 262, 571, 751
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 749
PkrI GCNGC 1 cut(s) 583
PleI GAGTC 3 cut(s) 280, 430, 699
PpsI GAGTC 3 cut(s) 280, 430, 699
PshBI ATTAAT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 496
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 351, 384
RruI TCGCGA 1 cut(s) 135
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 2 cut(s) 191, 680
SacI GAGCTC 1 cut(s) 496
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 3 cut(s) 303, 318, 501
SatI GCNGC 1 cut(s) 582
SchI GAGTC 3 cut(s) 281, 431, 700
ScrFI CCNGG 2 cut(s) 354, 387
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 1 cut(s) 55
SmiMI CAYNNNNRTG 2 cut(s) 191, 680
Sse9I AATT 7 cut(s) 243, 304, 374, 470, 503, 518, 544
SsiI CCGC 2 cut(s) 105, 582
SstI GAGCTC 1 cut(s) 496
StyD4I CCNGG 2 cut(s) 352, 385
TaaI ACNGT 2 cut(s) 575, 632
TaiI ACGT 4 cut(s) 259, 268, 318, 771
TaqI TCGA 3 cut(s) 141, 462, 530
TasI AATT 7 cut(s) 243, 304, 374, 470, 503, 518, 544
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 1 cut(s) 749
Tru1I TTAA 3 cut(s) 303, 318, 501
Tru9I TTAA 3 cut(s) 303, 318, 501
TscAI CASTG 3 cut(s) 159, 637, 687
TseFI GTSAC 2 cut(s) 257, 426
Tsp45I GTSAC 2 cut(s) 257, 426
TspDTI ATGAA 2 cut(s) 557, 591
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 3 cut(s) 159, 637, 687
VspI ATTAAT 1 cut(s) 303
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 3 cut(s) 374, 470, 503
XmiI GTMKAC 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 375
ZraI GACGTC 1 cut(s) 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.