Rroxscaffold_5G00350030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014814
Physical Location & Seq
Forward (+)
23560683 .. 23570508
9826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350030.1

Sequence Viewer

Length: 606 bp
ATGGCGACAAAGACAAAAGGAATGGGCTATATTTGGAGTGTTGTCCTGCATATTCTTTGTGTCATCAACTCTGTTTTGTTGTGGAGGAAAATTTCTGGGCTGCAGCCCGAGCACGACGGAAATCGCCTCTCAATCTTCCAGTGGCCATATCTCCCTCCACAGACCTCCGATCAACTTGATTCCAAAAGCATTTTCATTCGTCTTGAATGTACAGTGAGTAGTGTTGGGATGTCGGTCACCACCGACAAGGACTGCTGTCCCTCTAGCAAAGCAACCGGTCCCTCTCATCTCTCTCTCTTTTTCTCGCTTCTTCTTCTTCTTCCTCCTCCTGCAACAGCTTCCATTCTGCAACAATGGCAGCGTGTTGCAGCTGTAACATCACAGACCTCTCTCTTCCTCGGTCCAGTTCGTTCTCAAGCAGTTCGCATCTCTTCCTCGGTCCGTTCGTTCTCGCGCTTCGCATCTCTTCCTCGGTCCAGTTCGTTCTCAAAACAGTTCGCATCTTTCCTCGGTCCAGTTCGTTCTCAAAGCAGTTCTGCATCGGCCTCGCTCAGTTTCGTTTTGGATTGTGATGAGGAAGTGCAGCAAGGAAACGAAGATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

21.95

Weight (kDa)

8.93

Isoelectric Point (pI)

64.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 1 cut(s) 90
AfaI GTAC 1 cut(s) 211
AgeI ACCGGT 1 cut(s) 275
AgsI TTSAA 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 3 cut(s) 338, 371, 603
AluI AGCT 3 cut(s) 338, 371, 603
Alw21I GWGCWC 1 cut(s) 114
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 2 cut(s) 143, 543
ApeKI GCWGC 5 cut(s) 100, 103, 358, 368, 583
ApoI RAATTY 1 cut(s) 90
AsiGI ACCGGT 1 cut(s) 275
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 5 cut(s) 278, 401, 439, 474, 512
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 5 cut(s) 278, 401, 439, 474, 512
BalI TGGCCA 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 5 cut(s) 87, 115, 370, 380, 595
BcgI CGANNNNNNTGC 2 cut(s) 528, 562
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 5 cut(s) 101, 104, 359, 369, 584
BlsI GCNGC 5 cut(s) 102, 105, 360, 370, 585
Bme18I GGWCC 5 cut(s) 278, 401, 439, 474, 512
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 5 cut(s) 278, 401, 439, 474, 512
BmiI GGNNCC 1 cut(s) 280
BmsI GCATC 4 cut(s) 435, 470, 509, 548
BoxI GACNNNNGTC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 399
BsaJI CCNNGG 4 cut(s) 397, 435, 470, 508
BsaWI WCCGGW 1 cut(s) 275
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 4 cut(s) 139, 404, 477, 515
BseDI CCNNGG 4 cut(s) 397, 435, 470, 508
BseGI GGATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 565
BseNI ACTGG 4 cut(s) 139, 404, 477, 515
BseRI GAGGAG 1 cut(s) 315
BseXI GCAGC 5 cut(s) 87, 115, 370, 380, 595
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 2 cut(s) 145, 545
BshTI ACCGGT 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 2 cut(s) 243, 264
BsmFI GGGAC 2 cut(s) 243, 264
BsnI GGCC 2 cut(s) 145, 545
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 209
Bsp143I GATC 1 cut(s) 169
BspANI GGCC 2 cut(s) 145, 545
BspCNI CTCAG 1 cut(s) 564
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 280
BspMAI CTGCAG 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 209
BsrI ACTGG 4 cut(s) 139, 404, 477, 515
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 397, 435, 470, 508
BssMI GATC 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 214, 495
Bst6I CTCTTC 3 cut(s) 398, 436, 471
BstAUI TGTACA 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 551
BstEII GGTNACC 1 cut(s) 235
BstF5I GGATG 1 cut(s) 234
BstFNI CGCG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 1 cut(s) 172
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 109, 355
BstPAI GACNNNNGTC 1 cut(s) 255
BstPI GGTNACC 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 101
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 5 cut(s) 87, 115, 370, 380, 595
BsuRI GGCC 2 cut(s) 145, 545
BtsCI GGATG 1 cut(s) 234
BtsIMutI CAGTG 2 cut(s) 146, 219
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 5 cut(s) 278, 401, 439, 474, 512
CpoI CGGWCCG 1 cut(s) 439
Csp6I GTAC 1 cut(s) 210
CspAI ACCGGT 1 cut(s) 275
CspI CGGWCCG 1 cut(s) 439
CviJI RGCY 8 cut(s) 27, 100, 106, 145, 338, 371, 545, 603
CviKI_1 RGCY 8 cut(s) 27, 100, 106, 145, 338, 371, 545, 603
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 1 cut(s) 551
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 143
Eam1104I CTCTTC 3 cut(s) 398, 436, 471
EarI CTCTTC 3 cut(s) 398, 436, 471
Eco47I GGWCC 5 cut(s) 278, 401, 439, 474, 512
Eco88I CYCGRG 1 cut(s) 107
Eco91I GGTNACC 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 235
FaiI YATR 3 cut(s) 30, 51, 148
FaqI GGGAC 2 cut(s) 243, 264
Fnu4HI GCNGC 5 cut(s) 101, 104, 359, 369, 584
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 5 cut(s) 101, 104, 359, 369, 584
FspBI CTAG 1 cut(s) 264
GlaI GCGC 1 cut(s) 455
GluI GCNGC 5 cut(s) 101, 104, 359, 369, 584
HaeIII GGCC 2 cut(s) 145, 545
HapII CCGG 1 cut(s) 276
HhaI GCGC 1 cut(s) 456
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 276
HphI GGTGA 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 1 cut(s) 203
Hpy99I CGWCG 1 cut(s) 119
HpyCH4III ACNGT 2 cut(s) 214, 495
HpyCH4V TGCA 7 cut(s) 49, 103, 332, 349, 368, 539, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 355
HpyF3I CTNAG 1 cut(s) 551
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 1 cut(s) 169
LpnPI CCDG 8 cut(s) 59, 81, 152, 289, 342, 417, 490, 528
Lsp1109I GCAGC 5 cut(s) 87, 115, 370, 380, 595
LweI GCATC 4 cut(s) 435, 470, 509, 548
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 235, 373
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 8 cut(s) 127, 302, 305, 308, 311, 385, 423, 458
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 145
MluCI AATT 1 cut(s) 90
MluNI TGGCCA 1 cut(s) 145
Mox20I TGGCCA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 145
Msp20I TGGCCA 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 276
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 2 cut(s) 109, 355
NdeII GATC 1 cut(s) 169
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 443
PfeI GAWTC 1 cut(s) 179
PinAI ACCGGT 1 cut(s) 275
PkrI GCNGC 5 cut(s) 102, 105, 360, 370, 585
PshAI GACNNNNGTC 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 5 cut(s) 278, 401, 439, 474, 512
PstI CTGCAG 1 cut(s) 105
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
Rsr2I CGGWCCG 1 cut(s) 439
RsrII CGGWCCG 1 cut(s) 439
SatI GCNGC 5 cut(s) 101, 104, 359, 369, 584
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 5 cut(s) 278, 401, 439, 474, 512
SduI GDGCHC 1 cut(s) 114
SetI ASST 5 cut(s) 167, 340, 373, 389, 605
SfaNI GCATC 4 cut(s) 435, 470, 509, 548
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 5 cut(s) 278, 401, 439, 474, 512
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
Sse9I AATT 1 cut(s) 90
SspMI CTAG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 214, 495
TaqII GACCGA 5 cut(s) 223, 389, 427, 462, 500
TasI AATT 1 cut(s) 90
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 1 cut(s) 179
TscAI CASTG 2 cut(s) 146, 219
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 5 cut(s) 100, 103, 358, 368, 583
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 2 cut(s) 132, 431
TspRI CASTG 2 cut(s) 146, 219
VpaK11BI GGWCC 5 cut(s) 278, 401, 439, 474, 512
XapI RAATTY 1 cut(s) 90
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.