Rroxscaffold_3G00218550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014814
Physical Location & Seq
Forward (+)
821575 .. 827584
6010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218550.1

Sequence Viewer

Length: 501 bp
ATGGCCGGATCGCAGCCCGCCGGAATGCGGCAGCGTCCGGCCGGGAGAGAACTCACACCTGTACAGTGGGTAGTTGTTGGCCACCGACAAGGACTGCAGTCTCTCAAGCAAGACCGGCTGTCCGGTCGGCAATCCGGTTCGGCAGTCCGGTCGGCATCCGGTGGCAGCTGTCCGGCCGGGCAATCCGGCAGCCTGTCCGGTCGGCAATCCGGTTCAGCGAGTCCGCCGGCAATCCGTTCCAGCGGTCCGGTTCGGCGATACCGGTTCGAGCCCCTCCCCGCCTCTCTCACTCCCGACCGTGCGCCGTTCGCTCTTGTTCAAAGTTTCGTTCCCGCCTCCAATGGAGACTTCCGCAGGTATGTTCACACCGGTGATTTTCGGTTTCGCTTCGATGAAGCTAGACCCTTTTCTCTCGTAAATTTGTTGGTTCGTACGCCGTTTTCTTGGGTACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.09

Weight (kDa)

11.74

Isoelectric Point (pI)

71.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 345
AciI CCGC 7 cut(s) 18, 28, 224, 243, 279, 333, 352
AclWI GGATC 1 cut(s) 16
AcoI YGGCCR 4 cut(s) 3, 39, 79, 174
AcsI RAATTY 2 cut(s) 418, 469
AfaI GTAC 3 cut(s) 63, 433, 450
AfiI CCNNNNNNNGG 1 cut(s) 27
AgeI ACCGGT 2 cut(s) 261, 368
AgsI TTSAA 1 cut(s) 320
AhdI GACNNNNNGTC 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 2 cut(s) 168, 398
AluI AGCT 2 cut(s) 168, 398
Alw26I GTCTC 2 cut(s) 105, 339
AlwI GGATC 1 cut(s) 16
AoxI GGCC 4 cut(s) 3, 39, 79, 174
ApeKI GCWGC 4 cut(s) 13, 31, 165, 189
ApoI RAATTY 2 cut(s) 418, 469
AsiGI ACCGGT 2 cut(s) 261, 368
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 2 cut(s) 43, 178
AsuHPI GGTGA 1 cut(s) 383
AvaII GGWCC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 81
BanII GRGCYC 1 cut(s) 273
BbvI GCAGC 4 cut(s) 25, 43, 177, 201
BceAI ACGGC 2 cut(s) 289, 421
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcnI CCSGG 2 cut(s) 43, 178
BcoDI GTCTC 2 cut(s) 105, 339
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 5 cut(s) 14, 29, 32, 166, 190
BlsI GCNGC 5 cut(s) 15, 30, 33, 167, 191
Bme1390I CCNGG 2 cut(s) 43, 178
Bme18I GGWCC 1 cut(s) 245
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 245
BmrFI CCNGG 2 cut(s) 43, 178
BmsI GCATC 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 43, 178
BsaWI WCCGGW 9 cut(s) 122, 134, 147, 158, 197, 209, 247, 261, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 27
Bse118I RCCGGY 4 cut(s) 114, 226, 261, 368
BseGI GGATG 1 cut(s) 155
BseLI CCNNNNNNNGG 1 cut(s) 27
BseX3I CGGCCG 2 cut(s) 39, 174
BseXI GCAGC 4 cut(s) 25, 43, 177, 201
Bsh1285I CGRYCG 6 cut(s) 42, 127, 152, 177, 202, 298
BshFI GGCC 4 cut(s) 5, 41, 81, 176
BshTI ACCGGT 2 cut(s) 261, 368
BsiEI CGRYCG 6 cut(s) 42, 127, 152, 177, 202, 298
BsiWI CGTACG 1 cut(s) 431
BslI CCNNNNNNNGG 1 cut(s) 27
BsmAI GTCTC 2 cut(s) 105, 339
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 4 cut(s) 5, 41, 81, 176
Bsp1286I GDGCHC 1 cut(s) 273
Bsp1407I TGTACA 1 cut(s) 61
Bsp143I GATC 1 cut(s) 8
BspACI CCGC 7 cut(s) 18, 28, 224, 243, 279, 333, 352
BspANI GGCC 4 cut(s) 5, 41, 81, 176
BspMAI CTGCAG 1 cut(s) 99
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 1 cut(s) 16
BsrFI RCCGGY 4 cut(s) 114, 226, 261, 368
BsrGI TGTACA 1 cut(s) 61
BssAI RCCGGY 4 cut(s) 114, 226, 261, 368
BssMI GATC 1 cut(s) 8
Bst4CI ACNGT 2 cut(s) 66, 299
BstAUI TGTACA 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 18, 228
BstF5I GGATG 1 cut(s) 155
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 1 cut(s) 11
BstMAI GTCTC 2 cut(s) 105, 339
BstMBI GATC 1 cut(s) 8
BstMCI CGRYCG 6 cut(s) 42, 127, 152, 177, 202, 298
BstMWI GCNNNNNNNGC 2 cut(s) 115, 308
BstPAI GACNNNNGTC 1 cut(s) 97
BstSCI CCNGG 2 cut(s) 41, 176
BstSFI CTRYAG 1 cut(s) 95
BstV1I GCAGC 4 cut(s) 25, 43, 177, 201
BstZI CGGCCG 2 cut(s) 39, 174
BsuRI GGCC 4 cut(s) 5, 41, 81, 176
BtsCI GGATG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 71
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 2 cut(s) 18, 228
CfoI GCGC 1 cut(s) 304
Cfr10I RCCGGY 4 cut(s) 114, 226, 261, 368
Cfr13I GGNCC 1 cut(s) 245
CpoI CGGWCCG 1 cut(s) 245
CseI GACGC 1 cut(s) 23
Csp6I GTAC 3 cut(s) 62, 432, 449
CspAI ACCGGT 2 cut(s) 261, 368
CspI CGGWCCG 1 cut(s) 245
CviQI GTAC 3 cut(s) 62, 432, 449
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 4 cut(s) 3, 39, 79, 174
EagI CGGCCG 2 cut(s) 39, 174
Eam1105I GACNNNNNGTC 1 cut(s) 118
EciI GGCGGA 1 cut(s) 213
EclXI CGGCCG 2 cut(s) 39, 174
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 245
Eco52I CGGCCG 2 cut(s) 39, 174
EcoT38I GRGCYC 1 cut(s) 273
FaiI YATR 1 cut(s) 360
FauI CCCGC 3 cut(s) 25, 286, 340
Fnu4HI GCNGC 5 cut(s) 14, 29, 32, 166, 190
FokI GGATG 1 cut(s) 142
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 5 cut(s) 14, 29, 32, 166, 190
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 303
GluI GCNGC 5 cut(s) 14, 29, 32, 166, 190
HaeIII GGCC 4 cut(s) 5, 41, 81, 176
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 304
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HinfI GANTC 2 cut(s) 220, 493
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 364
Hpy188III TCNNGA 1 cut(s) 293
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 2 cut(s) 66, 299
HpyCH4V TGCA 2 cut(s) 97, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 308
HspAI GCGC 1 cut(s) 302
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Kzo9I GATC 1 cut(s) 8
Lsp1109I GCAGC 4 cut(s) 25, 43, 177, 201
LweI GCATC 1 cut(s) 164
MaeI CTAG 1 cut(s) 399
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 273
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 2 cut(s) 418, 469
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 229
MnlI CCTC 3 cut(s) 284, 292, 346
Mox20I TGGCCA 1 cut(s) 81
MroNI GCCGGC 1 cut(s) 226
MscI TGGCCA 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 369
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 168, 243
MspR9I CCNGG 2 cut(s) 43, 178
Mva1269I GAATGC 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 115, 308
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 2 cut(s) 43, 178
NdeII GATC 1 cut(s) 8
NgoMIV GCCGGC 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 369
PctI GAATGC 1 cut(s) 30
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 493
Pfl23II CGTACG 1 cut(s) 431
PinAI ACCGGT 2 cut(s) 261, 368
PkrI GCNGC 5 cut(s) 15, 30, 33, 167, 191
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PshAI GACNNNNGTC 1 cut(s) 97
PspLI CGTACG 1 cut(s) 431
PspPI GGNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 3 cut(s) 63, 433, 450
RsaNI GTAC 3 cut(s) 62, 432, 449
RseI CAYNNNNRTG 1 cut(s) 369
Rsr2I CGGWCCG 1 cut(s) 245
RsrII CGGWCCG 1 cut(s) 245
SatI GCNGC 5 cut(s) 14, 29, 32, 166, 190
Sau3AI GATC 1 cut(s) 8
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 2 cut(s) 43, 178
SduI GDGCHC 1 cut(s) 273
SetI ASST 4 cut(s) 61, 170, 359, 400
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 95
SgrAI CRCCGGYG 1 cut(s) 368
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 2 cut(s) 418, 469
SsiI CCGC 7 cut(s) 18, 28, 224, 243, 279, 333, 352
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 41, 176
TaaI ACNGT 2 cut(s) 66, 299
TaqI TCGA 2 cut(s) 267, 390
TasI AATT 2 cut(s) 418, 469
TatI WGTACW 1 cut(s) 61
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 1 cut(s) 493
TscAI CASTG 1 cut(s) 71
TseI GCWGC 4 cut(s) 13, 31, 165, 189
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 2 cut(s) 418, 469
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.