Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0013730

Physical Location & Seq

Genomic Coordinates Reverse (-)

91211509 .. 91212692

Gene Structure 1184 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00154690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGATCACGACCGGAAGTGCGGCAGAGTCCGAGTTGGAAAACCCTCCGGCGGTGAAATCAGGCTATTATCCACCTTCGGATAATTTCCCACCTTCAGCCATAGACACTACTTTCTTTACTCACGTCTACTATGCCTTTCTTGTGCCTAGCAACGTTACATTTAGATTTGAGCTGTCAAATGAAACGGCTTTACTCCTCTCGAACTTCACCACCGCCCTCCGCCACAAGACTCCGCCGGTGAAGACCATGTTGTCCATAGGTGGCGGTGCCGGAGAAGAAGTGGCACACATCTTTGCTAGCATGGCCTCAAACCCGTCCTCACGGTCAAACTTCATTGACTCGGCCATAGAGGTTGCACGTAAGTTTGGGTTTGATGGGCTGGACCTGGACTGGGAATACCCTGAAAGCCCAGTAGAAATGAAGGCCTTGGGCCACTTGCTAAAACAATGGCGTCGCGAAATCAAGAAAGAGGCCCAGGCCACAAATAGCCAACCTCTACTGCTCACCGCTGCCGTCTACTTCTCCGCCGAGTTCTTCTTGGATGCCGTTATCCGCTCGTACCCGATCAAGTACATGGAAAAGTACTTGGATTGGGCCAATGTAATGTGCTTCGACTATCACGGGGCTTGGAGCAATATTACAGGGCCCAATGCACCATTATTTGACCCAAATAGTAATGTGAACTCCGTTTACGGGCTTAGGTCATGGATCAACGGTGGCATGACCCCAAACAAGCTCGTAATGGGCTTGCCACTCTATGGCCGGACATGGGAGCTCAAAGACCCTAAAGATAACGGCATTGGATCCATTGCTATAGGCCCAGGCCCAGGAAATGGTTCACTCGGCTTCAACGAGTTGGAGGCCCTAAATGACGAGTCGAGCGCCACCGTGGTATATGACGCCGACACCGTATCAGTATATTCTGTTAATGGGTCGAGTTGGATTGCATTTGATGATGCCTTTACAACAACCTCCAAGACAGGGTTTGCTCAAGCTCTTGGGCTTCGTGGGTACTTCTTTTGGGCCATCGTTCATGATAGCAATTGGAAGATCTCGGCCCAAGCTTCAAAATCATGGATTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004568 GO:0005488 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006022 GO:0006026 GO:0006030 GO:0006032 GO:0006040 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0007586 GO:0008061 GO:0008144 GO:0008150 GO:0008152 GO:0008843 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016192 GO:0016787 GO:0016798 GO:0017144 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032501 GO:0032940 GO:0034774 GO:0035580 GO:0036230 GO:0042119 GO:0042581 GO:0042737 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044237 GO:0044245 GO:0044248 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046348 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060205 GO:0070013 GO:0070820 GO:0071704 GO:0097367 GO:0097708 GO:0099503 GO:1901071 GO:1901072 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1904724

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Glyco_hydro_18

Protein Analysis

361

Amino Acids

39.7

Weight (kDa)

5.04

Isoelectric Point (pI)

36.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_18	PF00704	21 - 346	6.8e-67	Glycosyl hydrolases family 18

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013730)

Species	Orthologous Gene IDs
malus_domestica	MD15G1156400.v1.1 MD15G1156500.v1.1 MD15G1156800.v1.1 MD15G1157100.v1.1
prunus_persica	Prupe.7G258400_v2.0.a1
pyrus_communis	pycom15g14090
rosa_chinensis	RchiOBHm_Chr2g0086331
rosa_laevigata	RLG00000015750
rosa_multiflora	Rmu_co8445245.1_g000001 Rmu_sc0004510.1_g000019 Rmu_sc0005127.1_g000013
rosa_roxburghii	Rroxscaffold_2G00154690
rosa_rugosa	Rorug01G0466600
rosa_samantha	Rh2AG016800 Rh2BG016600 Rh2CG017700 Rh2DG018400
rosa_wichuraiana	Rw2G001420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	250
AccB1I	GGYRCC	1 cut(s)	266
AccB7I	CCANNNNNTGG	2 cut(s)	758, 833
AccBSI	CCGCTC	1 cut(s)	555
AccI	GTMKAC	2 cut(s)	126, 516
AccII	CGCG	1 cut(s)	456
AciI	CCGC	9 cut(s)	20, 50, 213, 220, 233, 264, 507, 525, 553
AclI	AACGTT	1 cut(s)	153
AclWI	GGATC	3 cut(s)	716, 798, 811
AcoI	YGGCCR	2 cut(s)	342, 760
AcuI	CTGAAG	1 cut(s)	78
AcyI	GRCGYC	2 cut(s)	451, 900
AfaI	GTAC	4 cut(s)	560, 572, 584, 1013
AfiI	CCNNNNNNNGG	8 cut(s)	49, 391, 693, 758, 769, 827, 833, 981
AgsI	TTSAA	2 cut(s)	850, 1068
AjiI	CACGTC	1 cut(s)	124
AjnI	CCWGG	4 cut(s)	384, 474, 820, 826
AluBI	AGCT	5 cut(s)	172, 736, 775, 995, 1064
AluI	AGCT	5 cut(s)	172, 736, 775, 995, 1064
Alw21I	GWGCWC	1 cut(s)	777
AlwI	GGATC	3 cut(s)	716, 798, 811
ApaI	GGGCCC	1 cut(s)	648
ApeKI	GCWGC	1 cut(s)	509
Asp700I	GAANNNNTTC	1 cut(s)	835
AspLEI	GCGC	1 cut(s)	884
AsuHPI	GGTGA	4 cut(s)	64, 199, 250, 496
AsuNHI	GCTAGC	1 cut(s)	296
AvaII	GGWCC	1 cut(s)	382
BaeGI	GKGCMC	1 cut(s)	648
BamHI	GGATCC	1 cut(s)	803
BanI	GGYRCC	1 cut(s)	266
BanII	GRGCYC	2 cut(s)	648, 777
BbsI	GAAGAC	1 cut(s)	248
Bbv12I	GWGCWC	1 cut(s)	777
BbvI	GCAGC	1 cut(s)	496
BccI	CCATC	2 cut(s)	368, 1034
BceAI	ACGGC	4 cut(s)	201, 497, 530, 811
BciT130I	CCWGG	4 cut(s)	386, 476, 822, 828
BclI	TGATCA	1 cut(s)	3
BfaI	CTAG	2 cut(s)	147, 297
BfmI	CTRYAG	1 cut(s)	813
BfoI	RGCGCY	1 cut(s)	885
BglII	AGATCT	1 cut(s)	1050
BisI	GCNGC	2 cut(s)	21, 510
BlsI	GCNGC	2 cut(s)	22, 511
BmcAI	AGTACT	1 cut(s)	584
Bme1390I	CCNGG	4 cut(s)	386, 476, 822, 828
Bme18I	GGWCC	1 cut(s)	382
BmgBI	CACGTC	1 cut(s)	124
BmiI	GGNNCC	3 cut(s)	268, 646, 805
BmrFI	CCNGG	4 cut(s)	386, 476, 822, 828
BmrI	ACTGGG	2 cut(s)	400, 404
BmsI	GCATC	2 cut(s)	532, 946
BmtI	GCTAGC	1 cut(s)	300
BmuI	ACTGGG	2 cut(s)	400, 404
BpiI	GAAGAC	1 cut(s)	248
Bpu10I	CCTNAGC	1 cut(s)	698
BpuEI	CTTGAG	1 cut(s)	975
BsaAI	YACGTR	1 cut(s)	359
BsaHI	GRCGYC	2 cut(s)	451, 900
BsaJI	CCNNGG	5 cut(s)	426, 474, 820, 826, 888
BsaWI	WCCGGW	1 cut(s)	11
BsaXI	ACNNNNNCTCC	2 cut(s)	264, 294
Bsc4I	CCNNNNNNNGG	8 cut(s)	49, 391, 693, 758, 769, 827, 833, 981
Bse118I	RCCGGY	1 cut(s)	235
Bse1I	ACTGG	2 cut(s)	395, 410
Bse3DI	GCAATG	1 cut(s)	807
BseBI	CCWGG	4 cut(s)	386, 476, 822, 828
BseDI	CCNNGG	5 cut(s)	426, 474, 820, 826, 888
BseGI	GGATG	1 cut(s)	547
BseLI	CCNNNNNNNGG	8 cut(s)	49, 391, 693, 758, 769, 827, 833, 981
BseMI	GCAATG	1 cut(s)	807
BseNI	ACTGG	2 cut(s)	395, 410
BseRI	GAGGAG	1 cut(s)	185
BseSI	GKGCMC	1 cut(s)	648
BseXI	GCAGC	1 cut(s)	496
Bsh1236I	CGCG	1 cut(s)	456
Bsh1285I	CGRYCG	1 cut(s)	12
BshNI	GGYRCC	1 cut(s)	266
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	1 cut(s)	777
BsiSI	CCGG	5 cut(s)	12, 47, 236, 270, 763
BslI	CCNNNNNNNGG	8 cut(s)	49, 391, 693, 758, 769, 827, 833, 981
Bsp120I	GGGCCC	1 cut(s)	644
Bsp1286I	GDGCHC	2 cut(s)	648, 777
Bsp143I	GATC	5 cut(s)	3, 564, 708, 803, 1050
Bsp68I	TCGCGA	1 cut(s)	456
BspACI	CCGC	9 cut(s)	20, 50, 213, 220, 233, 264, 507, 525, 553
BspFNI	CGCG	1 cut(s)	456
BspHI	TCATGA	1 cut(s)	1033
BspLI	GGNNCC	3 cut(s)	268, 646, 805
BspOI	GCTAGC	1 cut(s)	300
BspPI	GGATC	3 cut(s)	716, 798, 811
BspT107I	GGYRCC	1 cut(s)	266
BsrBI	CCGCTC	1 cut(s)	555
BsrDI	GCAATG	1 cut(s)	807
BsrFI	RCCGGY	1 cut(s)	235
BsrI	ACTGG	2 cut(s)	395, 410
BssAI	RCCGGY	1 cut(s)	235
BssECI	CCNNGG	5 cut(s)	426, 474, 820, 826, 888
BssMI	GATC	5 cut(s)	3, 564, 708, 803, 1050
BssNI	GRCGYC	2 cut(s)	451, 900
BssT1I	CCWWGG	1 cut(s)	426
Bst2UI	CCWGG	4 cut(s)	386, 476, 822, 828
Bst4CI	ACNGT	4 cut(s)	324, 716, 889, 910
BstACI	GRCGYC	2 cut(s)	451, 900
BstBAI	YACGTR	1 cut(s)	359
BstC8I	GCNNGC	2 cut(s)	298, 749
BstDEI	CTNAG	1 cut(s)	698
BstDSI	CCRYGG	1 cut(s)	888
BstF5I	GGATG	1 cut(s)	547
BstFNI	CGCG	1 cut(s)	456
BstH2I	RGCGCY	1 cut(s)	885
BstHHI	GCGC	1 cut(s)	884
BstKTI	GATC	5 cut(s)	6, 567, 711, 806, 1053
BstMBI	GATC	5 cut(s)	3, 564, 708, 803, 1050
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	1 cut(s)	302
BstNI	CCWGG	4 cut(s)	386, 476, 822, 828
BstSCI	CCNGG	4 cut(s)	384, 474, 820, 826
BstSFI	CTRYAG	1 cut(s)	813
BstSLI	GKGCMC	1 cut(s)	648
BstUI	CGCG	1 cut(s)	456
BstV1I	GCAGC	1 cut(s)	496
BstV2I	GAAGAC	1 cut(s)	248
BstX2I	RGATCY	2 cut(s)	803, 1050
BstYI	RGATCY	2 cut(s)	803, 1050
BtgI	CCRYGG	1 cut(s)	888
BtrI	CACGTC	1 cut(s)	124
BtsCI	GGATG	1 cut(s)	547
BtuMI	TCGCGA	1 cut(s)	456
Cac8I	GCNNGC	2 cut(s)	298, 749
CciI	TCATGA	1 cut(s)	1033
CfoI	GCGC	1 cut(s)	884
Cfr10I	RCCGGY	1 cut(s)	235
CseI	GACGC	2 cut(s)	440, 908
Csp6I	GTAC	4 cut(s)	559, 571, 583, 1012
CviAII	CATG	8 cut(s)	247, 301, 574, 705, 721, 768, 1034, 1074
CviQI	GTAC	4 cut(s)	559, 571, 583, 1012
DdeI	CTNAG	1 cut(s)	698
DpnI	GATC	5 cut(s)	5, 566, 710, 805, 1052
DpnII	GATC	5 cut(s)	3, 564, 708, 803, 1050
DrdI	GACNNNNNNGTC	1 cut(s)	250
DseDI	GACNNNNNNGTC	1 cut(s)	250
EaeI	YGGCCR	2 cut(s)	342, 760
EciI	GGCGGA	3 cut(s)	209, 222, 514
Ecl136II	GAGCTC	1 cut(s)	775
Eco130I	CCWWGG	1 cut(s)	426
Eco147I	AGGCCT	1 cut(s)	425
Eco24I	GRGCYC	2 cut(s)	648, 777
Eco47I	GGWCC	1 cut(s)	382
Eco53kI	GAGCTC	1 cut(s)	775
Eco57I	CTGAAG	1 cut(s)	78
EcoICRI	GAGCTC	1 cut(s)	775
EcoO109I	RGGNCCY	2 cut(s)	644, 862
EcoRII	CCWGG	4 cut(s)	384, 474, 820, 826
EcoT14I	CCWWGG	1 cut(s)	426
EcoT38I	GRGCYC	2 cut(s)	648, 777
ErhI	CCWWGG	1 cut(s)	426
FaeI	CATG	8 cut(s)	250, 304, 577, 708, 724, 771, 1037, 1077
FatI	CATG	8 cut(s)	246, 300, 573, 704, 720, 767, 1033, 1073
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	2 cut(s)	126, 516
Fnu4HI	GCNGC	2 cut(s)	21, 510
FokI	GGATG	1 cut(s)	554
FriOI	GRGCYC	2 cut(s)	648, 777
Fsp4HI	GCNGC	2 cut(s)	21, 510
FspBI	CTAG	2 cut(s)	147, 297
GlaI	GCGC	1 cut(s)	883
GluI	GCNGC	2 cut(s)	21, 510
HaeII	RGCGCY	1 cut(s)	885
HapII	CCGG	5 cut(s)	12, 47, 236, 270, 763
HgaI	GACGC	2 cut(s)	440, 908
HhaI	GCGC	1 cut(s)	884
Hin1I	GRCGYC	2 cut(s)	451, 900
Hin1II	CATG	8 cut(s)	250, 304, 577, 708, 724, 771, 1037, 1077
Hin6I	GCGC	1 cut(s)	882
HinP1I	GCGC	1 cut(s)	882
HindIII	AAGCTT	1 cut(s)	1062
HinfI	GANTC	5 cut(s)	26, 229, 338, 875, 1078
HpaII	CCGG	5 cut(s)	12, 47, 236, 270, 763
HphI	GGTGA	4 cut(s)	64, 199, 250, 496
Hpy166II	GTNNAC	5 cut(s)	127, 517, 682, 691, 839
Hpy188I	TCNGA	2 cut(s)	31, 79
Hpy188III	TCNNGA	5 cut(s)	7, 199, 455, 463, 1034
Hpy8I	GTNNAC	5 cut(s)	127, 517, 682, 691, 839
Hpy99I	CGWCG	1 cut(s)	456
HpyAV	CCTTC	3 cut(s)	84, 102, 415
HpyCH4III	ACNGT	4 cut(s)	324, 716, 889, 910
HpyCH4IV	ACGT	3 cut(s)	123, 153, 358
HpyCH4V	TGCA	3 cut(s)	356, 653, 947
HpyF10VI	GCNNNNNNNGC	1 cut(s)	302
HpyF3I	CTNAG	1 cut(s)	698
HpySE526I	ACGT	3 cut(s)	123, 153, 358
Hsp92I	GRCGYC	2 cut(s)	451, 900
Hsp92II	CATG	8 cut(s)	250, 304, 577, 708, 724, 771, 1037, 1077
HspAI	GCGC	1 cut(s)	882
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	5 cut(s)	3, 564, 708, 803, 1050
LmnI	GCTCC	2 cut(s)	630, 772
Lsp1109I	GCAGC	1 cut(s)	496
LweI	GCATC	2 cut(s)	532, 946
MaeI	CTAG	2 cut(s)	147, 297
MaeII	ACGT	3 cut(s)	123, 153, 358
MaeIII	GTNAC	1 cut(s)	154
MalI	GATC	5 cut(s)	5, 566, 710, 805, 1052
MbiI	CCGCTC	1 cut(s)	555
MboI	GATC	5 cut(s)	3, 564, 708, 803, 1050
MboII	GAAGA	4 cut(s)	253, 287, 526, 1060
MfeI	CAATTG	1 cut(s)	1042
MflI	RGATCY	2 cut(s)	803, 1050
MhlI	GDGCHC	2 cut(s)	648, 777
MluCI	AATT	2 cut(s)	82, 1042
MlyI	GAGTC	4 cut(s)	35, 223, 332, 884
MmeI	TCCRAC	3 cut(s)	15, 837, 920
MroXI	GAANNNNTTC	1 cut(s)	835
MseI	TTAA	1 cut(s)	927
MspA1I	CMGCKG	1 cut(s)	509
MspI	CCGG	5 cut(s)	12, 47, 236, 270, 763
MspR9I	CCNGG	4 cut(s)	386, 476, 822, 828
MunI	CAATTG	1 cut(s)	1042
MvaI	CCWGG	4 cut(s)	386, 476, 822, 828
MvnI	CGCG	1 cut(s)	456
MwoI	GCNNNNNNNGC	1 cut(s)	302
NdeII	GATC	5 cut(s)	3, 564, 708, 803, 1050
NheI	GCTAGC	1 cut(s)	296
NlaIII	CATG	8 cut(s)	250, 304, 577, 708, 724, 771, 1037, 1077
NlaIV	GGNNCC	3 cut(s)	268, 646, 805
NmeAIII	GCCGAG	4 cut(s)	320, 553, 822, 1034
NruI	TCGCGA	1 cut(s)	456
PagI	TCATGA	1 cut(s)	1033
PceI	AGGCCT	1 cut(s)	425
PcsI	WCGNNNNNNNCGW	2 cut(s)	849, 906
PdmI	GAANNNNTTC	1 cut(s)	835
PfeI	GAWTC	1 cut(s)	1078
PflMI	CCANNNNNTGG	2 cut(s)	758, 833
PkrI	GCNGC	2 cut(s)	22, 511
PleI	GAGTC	4 cut(s)	34, 223, 332, 883
PpsI	GAGTC	4 cut(s)	34, 223, 332, 883
Ppu21I	YACGTR	1 cut(s)	359
Psp124BI	GAGCTC	1 cut(s)	777
Psp1406I	AACGTT	1 cut(s)	153
Psp6I	CCWGG	4 cut(s)	384, 474, 820, 826
PspGI	CCWGG	4 cut(s)	384, 474, 820, 826
PspN4I	GGNNCC	3 cut(s)	268, 646, 805
PspOMI	GGGCCC	1 cut(s)	644
PsrI	GAACNNNNNNTAC	2 cut(s)	674, 706
PsuI	RGATCY	2 cut(s)	803, 1050
RruI	TCGCGA	1 cut(s)	456
RsaI	GTAC	4 cut(s)	560, 572, 584, 1013
RsaNI	GTAC	4 cut(s)	559, 571, 583, 1012
SacI	GAGCTC	1 cut(s)	777
SaqAI	TTAA	1 cut(s)	927
SatI	GCNGC	2 cut(s)	21, 510
Sau3AI	GATC	5 cut(s)	3, 564, 708, 803, 1050
ScaI	AGTACT	1 cut(s)	584
SchI	GAGTC	4 cut(s)	35, 223, 332, 884
ScrFI	CCNGG	4 cut(s)	386, 476, 822, 828
SduI	GDGCHC	2 cut(s)	648, 777
SfaNI	GCATC	2 cut(s)	532, 946
SfcI	CTRYAG	1 cut(s)	813
SgrAI	CRCCGGYG	1 cut(s)	235
SinI	GGWCC	1 cut(s)	382
SmlI	CTYRAG	1 cut(s)	990
SmoI	CTYRAG	1 cut(s)	990
Sse9I	AATT	2 cut(s)	82, 1042
SseBI	AGGCCT	1 cut(s)	425
SsiI	CCGC	9 cut(s)	20, 50, 213, 220, 233, 264, 507, 525, 553
SspI	AATATT	1 cut(s)	637
SspMI	CTAG	2 cut(s)	147, 297
SstI	GAGCTC	1 cut(s)	777
StuI	AGGCCT	1 cut(s)	425
StyD4I	CCNGG	4 cut(s)	384, 474, 820, 826
StyI	CCWWGG	1 cut(s)	426
TaaI	ACNGT	4 cut(s)	324, 716, 889, 910
TaiI	ACGT	3 cut(s)	126, 156, 361
TaqI	TCGA	4 cut(s)	200, 612, 878, 935
TasI	AATT	2 cut(s)	82, 1042
TatI	WGTACW	2 cut(s)	570, 582
TauI	GCSGC	1 cut(s)	23
TfiI	GAWTC	1 cut(s)	1078
Tru1I	TTAA	1 cut(s)	927
Tru9I	TTAA	1 cut(s)	927
TseI	GCWGC	1 cut(s)	509
TspDTI	ATGAA	4 cut(s)	195, 322, 434, 1022
TspGWI	ACGGA	1 cut(s)	676
Van91I	CCANNNNNTGG	2 cut(s)	758, 833
VpaK11BI	GGWCC	1 cut(s)	382
XmiI	GTMKAC	2 cut(s)	126, 516
XmnI	GAANNNNTTC	1 cut(s)	835
XspI	CTAG	2 cut(s)	147, 297
ZrmI	AGTACT	1 cut(s)	584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.