Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0013730

Physical Location & Seq

Genomic Coordinates Reverse (-)

55902046 .. 55904997

Gene Structure 2952 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0466600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGATTAACTCGATGGATGAAGCCCCAGAAGTAAGTCTCACAGGTTACACTACTCATTATGGATATGGTCGGAAACCATCAGAAATGAGAAAAACGAAAAGGAGATTTAGTTCGTGGTGGACTAGTTTTTCGGTTCGAGGTTCATGGTGGGATTCGATATTTGTAGCCTCGTGTGTTATCGCAGTCTCACTGGATCCATTGTTCTTTTACATTCCTATCATCGATAGGGAAAAGAAGTGCCTTGAAGCAGACATAAAACTGAGGAACGTAACTCTTCTTTTGCGATCACTCATGGATATCACTTTTATAGTGCATATCTATCAAATTCGTGAAGCTATGATTGCTGCAGCCTCGGATAAGATGGCTACCCAGGAAGAATCGAAATTTTTCTGTTTCTTTGAATATGTTGATGCAATAATTGACAATTTGTCACGGCGCTCTCTCATAACTGACATTCTAGCTGTTCTTCCTATCCCACAGGTGCTACTACTTGTTGTCTTCTTCAAAATGAGAGGCTCTGGATATTTGGACCACAGGAAGATTTTGAACTTCTTTCTTCTCGCTCAATATTTGGCAAGAATTTATCGTATACACCTATCTTCGAAAAAACTTACAAAGAGTCATGGAATATGGATTAAGGGACTATTCAATTTCTTTCTCTATATCTTTGCAAGTCACGTACTTGGAGCCTTTTGGTATTTCTTTTCGATTCAACGAGAGACATCATGTTGGCATCGAAGTTGTGGTCACAACTGTACAAGTACCTTTTATTGTGATGAGTACAAACATTCAGACGATATGGTGACACTTATAACATCTCTGAATGCATCGTGCCCTATCAATGCTACTGGACCATTTGATTTTGGAATATTTCTTGATTCTCTGATGTCTAATAACACGGCATCAACCAACTTTCCACAAAGGTTGTGTTACTCTTTCTGGTGGGGTCTACGAAATTTGAGTAATTTTGGCACGAATCTTGTAACAAGTAACTATGTATGGGAGAATTTATTCGCAATTCTTATTTCTATCATTGGCTTGTTACTGTTTTTGTACCTCATCGGAAATGTGCAGACATTTATGTCTATGGAAACTGAGAAGTCAGAGGAGATAAGAAGGAAGATCAAATCAAAAGAGCAAGATATGAAAGTGTGGATGGAGAAAAATTGCCTCCCTGCTAATTTGAGGAAAGAAATCATGGCAAATATAAGAGAAAAATTAGAACAAGACAAGCATGCTGACATGGAGAATCTGTTCTCCATTCTTCCCTGGTCAACCAAAAAATCTCTAAAGCGTGTTCTCTGCATGAGTACACTGAGAAAAGTACCAATGCTTGAAGGTATGAATCCAAAAGTTTTGAAAATGATATGTGACTATCTGAAGCCTGTGATATACCCTGAGCATAGCTATATGTTGCCAAGGTGGATGAACCAGTTGATCGGATGGTGCTTGTCACCGAAGGAACTATGTGGATCTACTCGTCGAATACTAGTACTCCATCTAAGATCCACAGGACGGTTGAGAAAGGTGACATTTATGGAGATGAACAACTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004568 GO:0005488 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006022 GO:0006026 GO:0006030 GO:0006032 GO:0006040 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0007586 GO:0008061 GO:0008144 GO:0008150 GO:0008152 GO:0008843 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016192 GO:0016787 GO:0016798 GO:0017144 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032501 GO:0032940 GO:0034774 GO:0035580 GO:0036230 GO:0042119 GO:0042581 GO:0042737 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044237 GO:0044245 GO:0044248 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046348 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060205 GO:0070013 GO:0070820 GO:0071704 GO:0097367 GO:0097708 GO:0099503 GO:1901071 GO:1901072 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1904724

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Glyco_hydro_18

Protein Analysis

518

Amino Acids

60.6

Weight (kDa)

9.26

Isoelectric Point (pI)

45.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	49 - 368	7.1e-07	Ion transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013730)

Species	Orthologous Gene IDs
malus_domestica	MD15G1156400.v1.1 MD15G1156500.v1.1 MD15G1156800.v1.1 MD15G1157100.v1.1
prunus_persica	Prupe.7G258400_v2.0.a1
pyrus_communis	pycom15g14090
rosa_chinensis	RchiOBHm_Chr2g0086331
rosa_laevigata	RLG00000015750
rosa_multiflora	Rmu_co8445245.1_g000001 Rmu_sc0004510.1_g000019 Rmu_sc0005127.1_g000013
rosa_roxburghii	Rroxscaffold_2G00154690
rosa_rugosa	Rorug01G0466600
rosa_samantha	Rh2AG016800 Rh2BG016600 Rh2CG017700 Rh2DG018400
rosa_wichuraiana	Rw2G001420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	812
AasI	GACNNNNNNGTC	1 cut(s)	1081
AccI	GTMKAC	2 cut(s)	589, 949
AclWI	GGATC	4 cut(s)	188, 201, 1480, 1500
AcsI	RAATTY	5 cut(s)	324, 383, 579, 955, 1006
AcuI	CTGAAG	1 cut(s)	1400
AfaI	GTAC	8 cut(s)	681, 757, 763, 782, 1055, 1312, 1326, 1494
AfiI	CCNNNNNNNGG	1 cut(s)	1515
AgsI	TTSAA	8 cut(s)	245, 401, 505, 547, 649, 713, 1337, 1360
AhdI	GACNNNNNGTC	1 cut(s)	427
AhlI	ACTAGT	2 cut(s)	122, 1489
AjnI	CCWGG	2 cut(s)	369, 1268
AloI	GAACNNNNNNTCC	6 cut(s)	94, 126, 185, 217, 1238, 1270
AluBI	AGCT	3 cut(s)	335, 461, 1407
AluI	AGCT	3 cut(s)	335, 461, 1407
Alw26I	GTCTC	3 cut(s)	41, 190, 713
AlwI	GGATC	4 cut(s)	188, 201, 1480, 1500
ApeKI	GCWGC	2 cut(s)	344, 347
ApoI	RAATTY	5 cut(s)	324, 383, 579, 955, 1006
ArsI	GACNNNNNNTTYG	4 cut(s)	112, 144, 730, 762
Asp700I	GAANNNNTTC	4 cut(s)	386, 1010, 1253, 1550
AspLEI	GCGC	1 cut(s)	438
AspS9I	GGNCC	2 cut(s)	529, 851
AsuHPI	GGTGA	3 cut(s)	814, 1446, 1540
AsuII	TTCGAA	1 cut(s)	602
AvaII	GGWCC	2 cut(s)	529, 851
BaeGI	GKGCMC	1 cut(s)	836
BamHI	GGATCC	1 cut(s)	193
BauI	CACGAG	1 cut(s)	169
BbsI	GAAGAC	1 cut(s)	490
BbvI	GCAGC	2 cut(s)	331, 359
BccI	CCATC	6 cut(s)	7, 85, 355, 1150, 1437, 1506
BceAI	ACGGC	2 cut(s)	449, 915
BciT130I	CCWGG	2 cut(s)	371, 1270
BcoDI	GTCTC	3 cut(s)	41, 190, 713
BcuI	ACTAGT	2 cut(s)	122, 1489
BfaI	CTAG	3 cut(s)	123, 458, 1490
BfmI	CTRYAG	1 cut(s)	345
BfoI	RGCGCY	1 cut(s)	439
BisI	GCNGC	2 cut(s)	345, 348
BlsI	GCNGC	2 cut(s)	346, 349
BmcAI	AGTACT	1 cut(s)	1494
Bme1390I	CCNGG	2 cut(s)	371, 1270
Bme18I	GGWCC	2 cut(s)	529, 851
BmeRI	GACNNNNNGTC	1 cut(s)	427
BmgT120I	GGNCC	2 cut(s)	529, 851
BmiI	GGNNCC	2 cut(s)	195, 688
BmrFI	CCNGG	2 cut(s)	371, 1270
BmsI	GCATC	4 cut(s)	400, 742, 836, 911
BpiI	GAAGAC	1 cut(s)	490
Bpu10I	CCTNAGC	1 cut(s)	1398
Bpu14I	TTCGAA	1 cut(s)	602
Bsa29I	ATCGAT	1 cut(s)	222
BsaAI	YACGTR	1 cut(s)	679
BsaJI	CCNNGG	4 cut(s)	351, 369, 1268, 1418
BsaXI	ACNNNNNCTCC	6 cut(s)	94, 124, 1238, 1268, 1479, 1509
Bsc4I	CCNNNNNNNGG	1 cut(s)	1515
Bse1I	ACTGG	3 cut(s)	195, 853, 1432
BseBI	CCWGG	2 cut(s)	371, 1270
BseCI	ATCGAT	1 cut(s)	222
BseDI	CCNNGG	4 cut(s)	351, 369, 1268, 1418
BseGI	GGATG	4 cut(s)	22, 1161, 1431, 1448
BseLI	CCNNNNNNNGG	1 cut(s)	1515
BseMII	CTCAG	4 cut(s)	251, 1086, 1307, 1389
BseNI	ACTGG	3 cut(s)	195, 853, 1432
BseRI	GAGGAG	1 cut(s)	1121
BseSI	GKGCMC	1 cut(s)	836
BseXI	GCAGC	2 cut(s)	331, 359
BsgI	GTGCAG	1 cut(s)	1091
BshVI	ATCGAT	1 cut(s)	222
BslFI	GGGAC	1 cut(s)	654
BslI	CCNNNNNNNGG	1 cut(s)	1515
BsmAI	GTCTC	3 cut(s)	41, 190, 713
BsmFI	GGGAC	1 cut(s)	654
BsmI	GAATGC	1 cut(s)	829
Bsp119I	TTCGAA	1 cut(s)	602
Bsp1286I	GDGCHC	1 cut(s)	836
Bsp1407I	TGTACA	1 cut(s)	755
Bsp143I	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
BspCNI	CTCAG	4 cut(s)	252, 1087, 1308, 1390
BspDI	ATCGAT	1 cut(s)	222
BspLI	GGNNCC	2 cut(s)	195, 688
BspMAI	CTGCAG	1 cut(s)	349
BspPI	GGATC	4 cut(s)	188, 201, 1480, 1500
BspT104I	TTCGAA	1 cut(s)	602
BsrGI	TGTACA	1 cut(s)	755
BsrI	ACTGG	3 cut(s)	195, 853, 1432
BssECI	CCNNGG	4 cut(s)	351, 369, 1268, 1418
BssMI	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
BssNAI	GTATAC	1 cut(s)	590
BssSI	CACGAG	1 cut(s)	169
BssT1I	CCWWGG	1 cut(s)	1418
Bst1107I	GTATAC	1 cut(s)	590
Bst2BI	CACGAG	1 cut(s)	169
Bst2UI	CCWGG	2 cut(s)	371, 1270
Bst4CI	ACNGT	3 cut(s)	755, 1047, 1518
Bst6I	CTCTTC	1 cut(s)	279
BstAUI	TGTACA	1 cut(s)	755
BstBAI	YACGTR	1 cut(s)	679
BstBI	TTCGAA	1 cut(s)	602
BstC8I	GCNNGC	1 cut(s)	1236
BstDEI	CTNAG	5 cut(s)	260, 1095, 1316, 1398, 1502
BstF5I	GGATG	4 cut(s)	22, 1161, 1431, 1448
BstH2I	RGCGCY	1 cut(s)	439
BstHHI	GCGC	1 cut(s)	438
BstKTI	GATC	6 cut(s)	196, 287, 1125, 1440, 1475, 1508
BstMAI	GTCTC	3 cut(s)	41, 190, 713
BstMBI	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
BstMWI	GCNNNNNNNGC	1 cut(s)	341
BstNI	CCWGG	2 cut(s)	371, 1270
BstNSI	RCATGY	1 cut(s)	1238
BstSCI	CCNGG	2 cut(s)	369, 1268
BstSFI	CTRYAG	1 cut(s)	345
BstSLI	GKGCMC	1 cut(s)	836
BstV1I	GCAGC	2 cut(s)	331, 359
BstV2I	GAAGAC	1 cut(s)	490
BstX2I	RGATCY	3 cut(s)	193, 1472, 1505
BstYI	RGATCY	3 cut(s)	193, 1472, 1505
BstZ17I	GTATAC	1 cut(s)	590
Bsu15I	ATCGAT	1 cut(s)	222
BsuTUI	ATCGAT	1 cut(s)	222
BtsCI	GGATG	4 cut(s)	22, 1161, 1431, 1448
BtsIMutI	CAGTG	2 cut(s)	188, 1313
Cac8I	GCNNGC	1 cut(s)	1236
CfoI	GCGC	1 cut(s)	438
Cfr13I	GGNCC	2 cut(s)	529, 851
ClaI	ATCGAT	1 cut(s)	222
Csp6I	GTAC	8 cut(s)	680, 756, 762, 781, 1054, 1311, 1325, 1493
CspCI	CAANNNNNGTGG	2 cut(s)	906, 941
CviAII	CATG	8 cut(s)	144, 292, 623, 726, 1198, 1235, 1243, 1306
CviQI	GTAC	8 cut(s)	680, 756, 762, 781, 1054, 1311, 1325, 1493
DdeI	CTNAG	5 cut(s)	260, 1095, 1316, 1398, 1502
DpnI	GATC	6 cut(s)	195, 286, 1124, 1439, 1474, 1507
DpnII	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
DrdI	GACNNNNNNGTC	1 cut(s)	1081
DriI	GACNNNNNGTC	1 cut(s)	427
DseDI	GACNNNNNNGTC	1 cut(s)	1081
Eam1104I	CTCTTC	1 cut(s)	279
Eam1105I	GACNNNNNGTC	1 cut(s)	427
EarI	CTCTTC	1 cut(s)	279
Eco130I	CCWWGG	1 cut(s)	1418
Eco32I	GATATC	1 cut(s)	298
Eco47I	GGWCC	2 cut(s)	529, 851
Eco57I	CTGAAG	1 cut(s)	1400
EcoRII	CCWGG	2 cut(s)	369, 1268
EcoRV	GATATC	1 cut(s)	298
EcoT14I	CCWWGG	1 cut(s)	1418
EcoT22I	ATGCAT	1 cut(s)	829
ErhI	CCWWGG	1 cut(s)	1418
FaeI	CATG	8 cut(s)	147, 295, 626, 729, 1201, 1238, 1246, 1309
FaqI	GGGAC	1 cut(s)	654
FatI	CATG	8 cut(s)	143, 291, 622, 725, 1197, 1234, 1242, 1305
FblI	GTMKAC	2 cut(s)	589, 949
Fnu4HI	GCNGC	2 cut(s)	345, 348
FokI	GGATG	4 cut(s)	29, 1168, 1438, 1455
Fsp4HI	GCNGC	2 cut(s)	345, 348
FspBI	CTAG	3 cut(s)	123, 458, 1490
GlaI	GCGC	1 cut(s)	437
GluI	GCNGC	2 cut(s)	345, 348
HaeII	RGCGCY	1 cut(s)	439
HhaI	GCGC	1 cut(s)	438
Hin1II	CATG	8 cut(s)	147, 295, 626, 729, 1201, 1238, 1246, 1309
Hin6I	GCGC	1 cut(s)	436
HinP1I	GCGC	1 cut(s)	436
HincII	GTYRAC	1 cut(s)	1275
HindII	GTYRAC	1 cut(s)	1275
HinfI	GANTC	8 cut(s)	152, 377, 619, 709, 878, 976, 1249, 1345
HphI	GGTGA	3 cut(s)	814, 1446, 1540
Hpy166II	GTNNAC	5 cut(s)	120, 590, 950, 1275, 1313
Hpy188III	TCNNGA	3 cut(s)	329, 519, 875
Hpy8I	GTNNAC	5 cut(s)	120, 590, 950, 1275, 1313
Hpy99I	CGWCG	1 cut(s)	1485
HpyAV	CCTTC	3 cut(s)	1110, 1331, 1453
HpyCH4III	ACNGT	3 cut(s)	755, 1047, 1518
HpyCH4IV	ACGT	2 cut(s)	267, 678
HpyCH4V	TGCA	7 cut(s)	313, 347, 413, 671, 827, 1072, 1305
HpyF10VI	GCNNNNNNNGC	1 cut(s)	341
HpyF3I	CTNAG	5 cut(s)	260, 1095, 1316, 1398, 1502
HpySE526I	ACGT	2 cut(s)	267, 678
Hsp92II	CATG	8 cut(s)	147, 295, 626, 729, 1201, 1238, 1246, 1309
HspAI	GCGC	1 cut(s)	436
Kzo9I	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
LmnI	GCTCC	1 cut(s)	686
Lsp1109I	GCAGC	2 cut(s)	331, 359
LweI	GCATC	4 cut(s)	400, 742, 836, 911
MaeI	CTAG	3 cut(s)	123, 458, 1490
MaeII	ACGT	2 cut(s)	267, 678
MalI	GATC	6 cut(s)	195, 286, 1124, 1439, 1474, 1507
MboI	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
MflI	RGATCY	3 cut(s)	193, 1472, 1505
MhlI	GDGCHC	1 cut(s)	836
MlyI	GAGTC	1 cut(s)	628
MmeI	TCCRAC	1 cut(s)	50
MnlI	CCTC	9 cut(s)	131, 178, 255, 361, 506, 1067, 1099, 1179, 1181
Mph1103I	ATGCAT	1 cut(s)	829
MroXI	GAANNNNTTC	4 cut(s)	386, 1010, 1253, 1550
MseI	TTAA	2 cut(s)	6, 636
MspR9I	CCNGG	2 cut(s)	371, 1270
Mva1269I	GAATGC	1 cut(s)	829
MvaI	CCWGG	2 cut(s)	371, 1270
MwoI	GCNNNNNNNGC	1 cut(s)	341
NdeII	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
NlaIII	CATG	8 cut(s)	147, 295, 626, 729, 1201, 1238, 1246, 1309
NlaIV	GGNNCC	2 cut(s)	195, 688
NmuCI	GTSAC	7 cut(s)	429, 674, 746, 802, 1370, 1452, 1528
NsiI	ATGCAT	1 cut(s)	829
NspI	RCATGY	1 cut(s)	1238
NspV	TTCGAA	1 cut(s)	602
PaeI	GCATGC	1 cut(s)	1238
PctI	GAATGC	1 cut(s)	829
PdmI	GAANNNNTTC	4 cut(s)	386, 1010, 1253, 1550
PfeI	GAWTC	7 cut(s)	152, 377, 709, 878, 976, 1249, 1345
PkrI	GCNGC	2 cut(s)	346, 349
PleI	GAGTC	1 cut(s)	627
PpsI	GAGTC	1 cut(s)	627
Ppu21I	YACGTR	1 cut(s)	679
PsiI	TTATAA	1 cut(s)	812
Psp6I	CCWGG	2 cut(s)	369, 1268
PspGI	CCWGG	2 cut(s)	369, 1268
PspN4I	GGNNCC	2 cut(s)	195, 688
PspPI	GGNCC	2 cut(s)	529, 851
PstI	CTGCAG	1 cut(s)	349
PsuI	RGATCY	3 cut(s)	193, 1472, 1505
RsaI	GTAC	8 cut(s)	681, 757, 763, 782, 1055, 1312, 1326, 1494
RsaNI	GTAC	8 cut(s)	680, 756, 762, 781, 1054, 1311, 1325, 1493
SaqAI	TTAA	2 cut(s)	6, 636
SatI	GCNGC	2 cut(s)	345, 348
Sau3AI	GATC	6 cut(s)	193, 284, 1122, 1437, 1472, 1505
Sau96I	GGNCC	2 cut(s)	529, 851
ScaI	AGTACT	1 cut(s)	1494
SchI	GAGTC	1 cut(s)	628
ScrFI	CCNGG	2 cut(s)	371, 1270
SduI	GDGCHC	1 cut(s)	836
SfaNI	GCATC	4 cut(s)	400, 742, 836, 911
SfcI	CTRYAG	1 cut(s)	345
SfuI	TTCGAA	1 cut(s)	602
SinI	GGWCC	2 cut(s)	529, 851
SpeI	ACTAGT	2 cut(s)	122, 1489
SphI	GCATGC	1 cut(s)	1238
SspI	AATATT	2 cut(s)	569, 870
SspMI	CTAG	3 cut(s)	123, 458, 1490
StyD4I	CCNGG	2 cut(s)	369, 1268
StyI	CCWWGG	1 cut(s)	1418
TaaI	ACNGT	3 cut(s)	755, 1047, 1518
TaiI	ACGT	2 cut(s)	270, 681
TaqI	TCGA	9 cut(s)	11, 136, 155, 222, 380, 602, 707, 736, 1483
TatI	WGTACW	4 cut(s)	755, 780, 1310, 1492
TfiI	GAWTC	7 cut(s)	152, 377, 709, 878, 976, 1249, 1345
Tru1I	TTAA	2 cut(s)	6, 636
Tru9I	TTAA	2 cut(s)	6, 636
TscAI	CASTG	2 cut(s)	195, 1320
TseFI	GTSAC	7 cut(s)	429, 674, 746, 802, 1370, 1452, 1528
TseI	GCWGC	2 cut(s)	344, 347
Tsp45I	GTSAC	7 cut(s)	429, 674, 746, 802, 1370, 1452, 1528
TspDTI	ATGAA	5 cut(s)	33, 132, 1160, 1358, 1442
TspRI	CASTG	2 cut(s)	195, 1320
VpaK11BI	GGWCC	2 cut(s)	529, 851
XapI	RAATTY	5 cut(s)	324, 383, 579, 955, 1006
XceI	RCATGY	1 cut(s)	1238
XmiI	GTMKAC	2 cut(s)	589, 949
XmnI	GAANNNNTTC	4 cut(s)	386, 1010, 1253, 1550
XspI	CTAG	3 cut(s)	123, 458, 1490
ZrmI	AGTACT	1 cut(s)	1494
Zsp2I	ATGCAT	1 cut(s)	829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.