Rroxscaffold_3G00225130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004860
Physical Location & Seq
Reverse (-)
8352608 .. 8353468
861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225130.1

Sequence Viewer

Length: 606 bp
ATGGGCGATTCTGTTTCAGGGTTGATTCTCCTCTCGGAGGTTGCAGATCTTGCTGCGTCTGATTCCAACACAAGGGCTTCTTCTTCTTCTTTGAAATTTTGCGATGTTGCGATTCAGATGTTGATGGAGTTCAAGGCTATGAGGAAGAAAGGCTTGCTCGCCGCTCATCTGGAGAAGGCCTTCTTCATTCCCAAGAAGAAAAGGTCCAATCTTTCCATTTCTATTTCTATTTCTAAGAGGAGGACTGACTTGTTGGCTTTCTGTATACCCAAAAAGAAAAGATCTAATCTTGTTTCTCATATGATGATGAAAAGGAAGAGATCTGGGGAGCAAGATGGTCAGATTCCAAGACTAAAGAGGCGGAGAACTATTACTGGAGATGTCTTGGGTTCTCAATTGGCCATGCCCTCTCGTTCTCATCAAGAAATGAAAAACATGATTAGCAGCCACGTCCCACTCTGGTCATTCCAAAGGAAATCCCCGCTTCGATTTTCCCTCATCAAGCTTCATCTATCTGCCCCCAGGACAACTGCGAGACTTCCAGCAAAGAAGAGATCATGGTTTTCAAAGCTGGGGTACATTCTTGAGACAACCATAAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.77

Weight (kDa)

11.16

Isoelectric Point (pI)

58.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 265
AciI CCGC 3 cut(s) 162, 361, 482
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 95
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 2 cut(s) 37, 72
AgsI TTSAA 3 cut(s) 94, 133, 567
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 2 cut(s) 505, 571
AluI AGCT 2 cut(s) 505, 571
Alw26I GTCTC 2 cut(s) 529, 581
AoxI GGCC 2 cut(s) 177, 399
ApeKI GCWGC 2 cut(s) 53, 444
ApoI RAATTY 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 204
AvaII GGWCC 1 cut(s) 204
BalI TGGCCA 1 cut(s) 401
BbvI GCAGC 2 cut(s) 40, 456
BccI CCATC 2 cut(s) 118, 329
BciT130I CCWGG 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 529, 581
BglII AGATCT 3 cut(s) 46, 281, 320
BisI GCNGC 3 cut(s) 54, 162, 445
BlsI GCNGC 3 cut(s) 55, 163, 446
Bme1390I CCNGG 1 cut(s) 523
Bme18I GGWCC 1 cut(s) 204
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 523
BpmI CTGGAG 2 cut(s) 191, 396
BpuEI CTTGAG 1 cut(s) 605
BsaJI CCNNGG 1 cut(s) 521
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 72
Bse1I ACTGG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 1 cut(s) 521
BseLI CCNNNNNNNGG 2 cut(s) 37, 72
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 2 cut(s) 20, 253
BseXI GCAGC 2 cut(s) 40, 456
BseYI CCCAGC 1 cut(s) 571
BshFI GGCC 2 cut(s) 179, 401
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 2 cut(s) 37, 72
BsmAI GTCTC 2 cut(s) 529, 581
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 2 cut(s) 179, 401
Bsp143I GATC 4 cut(s) 46, 281, 320, 554
BspACI CCGC 3 cut(s) 162, 361, 482
BspANI GGCC 2 cut(s) 179, 401
BsrBI CCGCTC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 379
BssECI CCNNGG 1 cut(s) 521
BssMI GATC 4 cut(s) 46, 281, 320, 554
BssNAI GTATAC 1 cut(s) 266
Bst1107I GTATAC 1 cut(s) 266
Bst2UI CCWGG 1 cut(s) 523
Bst6I CTCTTC 2 cut(s) 311, 545
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 155, 159
BstDEI CTNAG 1 cut(s) 234
BstENI CCTNNNNNAGG 1 cut(s) 35
BstKTI GATC 4 cut(s) 49, 284, 323, 557
BstMAI GTCTC 2 cut(s) 529, 581
BstMBI GATC 4 cut(s) 46, 281, 320, 554
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 40, 456
BstX2I RGATCY 3 cut(s) 46, 281, 320
BstYI RGATCY 3 cut(s) 46, 281, 320
BstZ17I GTATAC 1 cut(s) 266
BsuRI GGCC 2 cut(s) 179, 401
BtgZI GCGATG 1 cut(s) 117
BtrI CACGTC 1 cut(s) 451
Cac8I GCNNGC 2 cut(s) 155, 159
Cfr13I GGNCC 1 cut(s) 204
CseI GACGC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 577
CviAII CATG 3 cut(s) 403, 436, 558
CviJI RGCY 9 cut(s) 77, 137, 153, 179, 257, 401, 447, 505, 571
CviKI_1 RGCY 9 cut(s) 77, 137, 153, 179, 257, 401, 447, 505, 571
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 1 cut(s) 234
DpnI GATC 4 cut(s) 48, 283, 322, 556
DpnII GATC 4 cut(s) 46, 281, 320, 554
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 2 cut(s) 311, 545
EarI CTCTTC 2 cut(s) 311, 545
EciI GGCGGA 1 cut(s) 376
Eco147I AGGCCT 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 204
EcoNI CCTNNNNNAGG 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 521
FaeI CATG 3 cut(s) 406, 439, 561
FaiI YATR 8 cut(s) 140, 266, 300, 302, 404, 437, 559, 596
FalI AAGNNNNNCTT 6 cut(s) 64, 96, 137, 169, 167, 199
FaqI GGGAC 1 cut(s) 437
FatI CATG 3 cut(s) 402, 435, 557
FauI CCCGC 1 cut(s) 489
FauNDI CATATG 1 cut(s) 300
FblI GTMKAC 1 cut(s) 265
Fnu4HI GCNGC 3 cut(s) 54, 162, 445
Fsp4HI GCNGC 3 cut(s) 54, 162, 445
GluI GCNGC 3 cut(s) 54, 162, 445
GsaI CCCAGC 1 cut(s) 575
GsuI CTGGAG 2 cut(s) 191, 396
HaeIII GGCC 2 cut(s) 179, 401
HgaI GACGC 1 cut(s) 45
Hin1II CATG 3 cut(s) 406, 439, 561
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 5 cut(s) 8, 25, 62, 112, 343
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 4 cut(s) 37, 61, 117, 342
Hpy188III TCNNGA 3 cut(s) 170, 422, 584
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 169, 190
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 3 cut(s) 406, 439, 561
Kzo9I GATC 4 cut(s) 46, 281, 320, 554
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 9 cut(s) 3, 155, 309, 360, 445, 508, 535, 555, 557
Lsp1109I GCAGC 2 cut(s) 40, 456
MaeII ACGT 1 cut(s) 450
MalI GATC 4 cut(s) 48, 283, 322, 556
MbiI CCGCTC 1 cut(s) 164
MboI GATC 4 cut(s) 46, 281, 320, 554
MboII GAAGA 8 cut(s) 72, 75, 78, 157, 175, 208, 328, 562
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 3 cut(s) 46, 281, 320
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 2 cut(s) 95, 395
MluNI TGGCCA 1 cut(s) 401
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 8 cut(s) 31, 41, 135, 231, 234, 351, 418, 506
Mox20I TGGCCA 1 cut(s) 401
MroXI GAANNNNTTC 1 cut(s) 179
MscI TGGCCA 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 523
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 523
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeI CATATG 1 cut(s) 300
NdeII GATC 4 cut(s) 46, 281, 320, 554
NlaIII CATG 3 cut(s) 406, 439, 561
PceI AGGCCT 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 5 cut(s) 8, 25, 62, 112, 343
PkrI GCNGC 3 cut(s) 55, 163, 446
Psp6I CCWGG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 521
PspPI GGNCC 1 cut(s) 204
PsuI RGATCY 3 cut(s) 46, 281, 320
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SatI GCNGC 3 cut(s) 54, 162, 445
Sau3AI GATC 4 cut(s) 46, 281, 320, 554
Sau96I GGNCC 1 cut(s) 204
ScrFI CCNGG 1 cut(s) 523
SetI ASST 5 cut(s) 42, 206, 453, 507, 573
SinI GGWCC 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 2 cut(s) 95, 395
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 3 cut(s) 162, 361, 482
StuI AGGCCT 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 521
TaiI ACGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 487
TasI AATT 2 cut(s) 95, 395
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 5 cut(s) 8, 25, 62, 112, 343
TseI GCWGC 2 cut(s) 53, 444
TspDTI ATGAA 4 cut(s) 175, 323, 443, 497
VpaK11BI GGWCC 1 cut(s) 204
XagI CCTNNNNNAGG 1 cut(s) 35
XapI RAATTY 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.