Rroxscaffold_3G00225440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004860
Physical Location & Seq
Forward (+)
8695668 .. 8696661
994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225440.1

Sequence Viewer

Length: 531 bp
ATGTGTACTCAACCTGAGATAGTCCAAGTTTGGAGGTCTGTTCAAAACAAGGGGAATATAAGCACTGAGGTTGACAGAATTACTTCTAGTGGTGCAAGGAAAAATAAGTGTTTTCAACTTGATCCTGTTGAGTCTAAGGGGAAATCTGTTGCTTCAAAAAATTTGGTATGGGCTGAAAAGAAAACTATTGCAACCTCTGAAATCACTGTTGATTTCACTTCTCTCCCTGCTATTGATGCTGGTTCTAGTAATCATCTTAGAGCTGCCATAGAAGTAATGGTGAATGCTGTCAATAGAGCTGCTGATGGTGTTAAGCAGCTTGTTTTTCTTAATTATTACATAGTGGAGGGGCACACTTGCCTCTGTCTTTTCATGATGGAAATCATCATCATGGCCAGCGCTGCAGCCCAATCCAACTGGAGATATGATACATATACACTGGATCAGAATCGCATCAACTTGGAAGGGGATGGTATTAGTGAGGTGCAAGGCTGTATTGTTATGGAACAGCAGCTCCCCCTCCCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.4

Weight (kDa)

5.41

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 116, 450
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 160
AfaI GTAC 1 cut(s) 7
AfeI AGCGCT 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 524
AgsI TTSAA 3 cut(s) 44, 116, 156
AjnI CCWGG 1 cut(s) 523
AloI GAACNNNNNNTCC 2 cut(s) 498, 530
AluBI AGCT 4 cut(s) 263, 299, 319, 514
AluI AGCT 4 cut(s) 263, 299, 319, 514
AlwI GGATC 2 cut(s) 116, 450
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 6 cut(s) 263, 299, 316, 401, 404, 511
ApoI RAATTY 1 cut(s) 160
Asp700I GAANNNNTTC 1 cut(s) 82
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 354
BalI TGGCCA 1 cut(s) 395
BbvI GCAGC 6 cut(s) 250, 286, 328, 388, 416, 523
BccI CCATC 3 cut(s) 299, 370, 464
BciT130I CCWGG 1 cut(s) 525
BfaI CTAG 2 cut(s) 87, 246
BfmI CTRYAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 6 cut(s) 264, 300, 317, 402, 405, 512
BlsI GCNGC 6 cut(s) 265, 301, 318, 403, 406, 513
Bme1390I CCNGG 1 cut(s) 525
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 226, 462
BpmI CTGGAG 1 cut(s) 439
BsaBI GATNNNNATC 2 cut(s) 380, 447
BsaJI CCNNGG 1 cut(s) 523
BsaXI ACNNNNNCTCC 2 cut(s) 498, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 524
Bse1I ACTGG 2 cut(s) 422, 444
Bse8I GATNNNNATC 2 cut(s) 380, 447
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 1 cut(s) 523
BseGI GGATG 1 cut(s) 475
BseJI GATNNNNATC 2 cut(s) 380, 447
BseLI CCNNNNNNNGG 1 cut(s) 524
BseMII CTCAG 2 cut(s) 6, 57
BseNI ACTGG 2 cut(s) 422, 444
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 6 cut(s) 250, 286, 328, 388, 416, 523
BshFI GGCC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 524
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 2 cut(s) 121, 442
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 7, 58
BspHI TCATGA 1 cut(s) 372
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 2 cut(s) 116, 450
BsrI ACTGG 2 cut(s) 422, 444
BssECI CCNNGG 1 cut(s) 523
BssMI GATC 2 cut(s) 121, 442
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 4 cut(s) 15, 66, 135, 257
BstF5I GGATG 1 cut(s) 475
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 2 cut(s) 124, 445
BstMBI GATC 2 cut(s) 121, 442
BstMWI GCNNNNNNNGC 2 cut(s) 236, 401
BstNI CCWGG 1 cut(s) 525
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 354
BstV1I GCAGC 6 cut(s) 250, 286, 328, 388, 416, 523
BsuRI GGCC 1 cut(s) 395
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 3 cut(s) 63, 204, 437
Cac8I GCNNGC 1 cut(s) 397
CciI TCATGA 1 cut(s) 372
CfoI GCGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 6
CviAII CATG 2 cut(s) 373, 391
CviJI RGCY 8 cut(s) 173, 263, 299, 319, 395, 407, 492, 514
CviKI_1 RGCY 8 cut(s) 173, 263, 299, 319, 395, 407, 492, 514
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 4 cut(s) 15, 66, 135, 257
DpnI GATC 2 cut(s) 123, 444
DpnII GATC 2 cut(s) 121, 442
EaeI YGGCCR 1 cut(s) 393
Eco47III AGCGCT 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 523
FaeI CATG 2 cut(s) 376, 394
FatI CATG 2 cut(s) 372, 390
Fnu4HI GCNGC 6 cut(s) 264, 300, 317, 402, 405, 512
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 6 cut(s) 264, 300, 317, 402, 405, 512
FspBI CTAG 2 cut(s) 87, 246
GlaI GCGC 1 cut(s) 400
GluI GCNGC 6 cut(s) 264, 300, 317, 402, 405, 512
GsuI CTGGAG 1 cut(s) 439
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 1 cut(s) 395
HhaI GCGC 1 cut(s) 401
Hin1II CATG 2 cut(s) 376, 394
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 2 cut(s) 131, 448
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 6, 73
Hpy188I TCNGA 2 cut(s) 199, 447
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 2 cut(s) 6, 73
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 4 cut(s) 95, 191, 404, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 401
HpyF3I CTNAG 4 cut(s) 15, 66, 135, 257
Hsp92II CATG 2 cut(s) 376, 394
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 2 cut(s) 121, 442
LmnI GCTCC 1 cut(s) 519
LpnPI CCDG 8 cut(s) 27, 138, 225, 240, 403, 409, 425, 510
Lsp1109I GCAGC 6 cut(s) 250, 286, 328, 388, 416, 523
LweI GCATC 2 cut(s) 226, 462
MaeI CTAG 2 cut(s) 87, 246
MalI GATC 2 cut(s) 123, 444
MboI GATC 2 cut(s) 121, 442
MhlI GDGCHC 1 cut(s) 354
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 3 cut(s) 78, 160, 331
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 7 cut(s) 27, 61, 205, 340, 371, 475, 530
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 82
MscI TGGCCA 1 cut(s) 395
MseI TTAA 2 cut(s) 312, 330
MslI CAYNNNNRTG 1 cut(s) 389
Msp20I TGGCCA 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 525
MwoI GCNNNNNNNGC 2 cut(s) 236, 401
NdeII GATC 2 cut(s) 121, 442
NlaIII CATG 2 cut(s) 376, 394
PagI TCATGA 1 cut(s) 372
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 82
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 6 cut(s) 265, 301, 318, 403, 406, 513
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PstI CTGCAG 1 cut(s) 406
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 2 cut(s) 312, 330
SatI GCNGC 6 cut(s) 264, 300, 317, 402, 405, 512
Sau3AI GATC 2 cut(s) 121, 442
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 1 cut(s) 354
SetI ASST 9 cut(s) 16, 38, 72, 197, 265, 301, 321, 486, 516
SfaNI GCATC 2 cut(s) 226, 462
SfcI CTRYAG 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 389
Sse9I AATT 3 cut(s) 78, 160, 331
SspMI CTAG 2 cut(s) 87, 246
StyD4I CCNGG 1 cut(s) 523
TaaI ACNGT 1 cut(s) 208
TasI AATT 3 cut(s) 78, 160, 331
TatI WGTACW 1 cut(s) 5
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 2 cut(s) 312, 330
Tru9I TTAA 2 cut(s) 312, 330
TscAI CASTG 3 cut(s) 70, 211, 444
TseI GCWGC 6 cut(s) 263, 299, 316, 401, 404, 511
TspDTI ATGAA 1 cut(s) 361
TspRI CASTG 3 cut(s) 70, 211, 444
XapI RAATTY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 82
XspI CTAG 2 cut(s) 87, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.