Rorug07G0296800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004860
Physical Location & Seq
Reverse (-)
29221893 .. 29225716
3824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0296800.1

Sequence Viewer

Length: 1167 bp
ATGGCGGAAAGTGGAATTGAAATAGAGAAAATAGAGATGTTGGATCCAGAGTCAGAGTTCCTGGCGTCGAATAGGGAGTCAGGGAACGAGTGGGAGCTGTTCAAGGAGAACGTGCGGCCATTGAAGAGAGGCCGCAAAGTTGAAATCTTAAACGACGCTCTCAAAGCTCAAACTGACAACCAACTCAAGAAGTCCCTCTTTGAACAACGAAGGCGGTTGATTCAAGCAATTGACGAGTATGAAGGAGATGACCCTCTGCAGCCGTGGCTCGAGTGTATCAAGTGGGTTCAGGAAGCATTTCCCCCTGGTGGGGATTTCTCGGGACTGGTTGTGATATATGAGCAATGTGTGCGTACATTTTGGCATTCAGACCGCTACAAGGATGATCTCCGTTATCTCAAAGTCTGGTTGGAATATGCTGAAAATTGTGCGGATGCTGAAGTCATATTCAGTTTTCTAGATGCAAATGAAATTGGAAAGACACATTCTCAGTATTACATATCTTATGCTTTGCTCATGGAATCCAAGAACAAAGTGAAAGCTGCCAATGAAATATTAAATCTTGGGATATCAAGGAATGCTCAGCCCACAGAAAAATTGAATGATGCATTTAGGAAATTTCTTGCACGCTCAATGAGAAGACCACCAAAAGCTCCAGAGGAGGATTCAGCTGAGAAACGCTTACCCACCCGAAGCTTTGGAACTGTCCTTGCCAAGGGAGAGAATCGTCTGCAGACAAGTGAGAGCTCTGGTTTGTTGAAGAAGAACGTGAAGCTAGATAGGGGTCGTGTTCCACTTAGCATTTATAAAGAAGATTCAGATTCCCTTGACCAATCAGAAGTGTCAAAGAGAGACGTGAATTCTTGGCACACCCTTGGACCTAGAGCAGAGAGAAATAAAGAAAATAGTGCGATCCCTTCCAAGTGGACATCATACAAGGTTCGTCAAAGACCTGTGCCTAGAATCGGAGGAGCAGCTGCTACTAGTGCCTGCATTGAGATTTTTGTCGATGAAGAATGTGAAGAGACACATAGAATGAATGCTGAGGGTGCCAAATCTTCAACCTTGCAGCCTAGACAGGGTGAAGAGCAAGAGCTCAAGAAGGAAACAGAGTTGTTGAGACAGCAACCATTACGCAATTTCCCTCAAAATAGTCTTCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

44.66

Weight (kDa)

6.05

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 68 - 190 2.1e-43 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 1049
AciI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
AclWI GGATC 3 cut(s) 38, 51, 907
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 2 cut(s) 617, 859
AcuI CTGAAG 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
AgsI TTSAA 9 cut(s) 20, 103, 124, 143, 203, 224, 601, 760, 1062
AhlI ACTAGT 1 cut(s) 983
AjiI CACGTC 1 cut(s) 856
AjnI CCWGG 2 cut(s) 60, 304
Alw21I GWGCWC 2 cut(s) 749, 1098
Alw26I GTCTC 3 cut(s) 846, 1019, 1114
AlwI GGATC 3 cut(s) 38, 51, 907
Ama87I CYCGRG 2 cut(s) 269, 319
AoxI GGCC 2 cut(s) 116, 130
ApeKI GCWGC 5 cut(s) 259, 542, 974, 977, 1069
ApoI RAATTY 2 cut(s) 617, 859
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 878
AsuHPI GGTGA 1 cut(s) 1094
AvaI CYCGRG 2 cut(s) 269, 319
AvaII GGWCC 1 cut(s) 878
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 1049
BanII GRGCYC 2 cut(s) 749, 1098
BbsI GAAGAC 2 cut(s) 646, 1148
Bbv12I GWGCWC 2 cut(s) 749, 1098
BbvCI CCTCAGC 1 cut(s) 1044
BbvI GCAGC 5 cut(s) 271, 529, 964, 986, 1081
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 2 cut(s) 62, 306
BcoDI GTCTC 3 cut(s) 846, 1019, 1114
BcuI ACTAGT 1 cut(s) 983
BfaI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
BfmI CTRYAG 2 cut(s) 257, 731
BisI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 7 cut(s) 117, 134, 261, 544, 976, 979, 1071
Bme1390I CCNGG 2 cut(s) 62, 306
Bme18I GGWCC 1 cut(s) 878
BmeT110I CYCGRG 2 cut(s) 269, 319
BmgBI CACGTC 1 cut(s) 856
BmgT120I GGNCC 1 cut(s) 878
BmiI GGNNCC 2 cut(s) 45, 1051
BmrFI CCNGG 2 cut(s) 62, 306
BmsI GCATC 3 cut(s) 424, 451, 595
BpiI GAAGAC 2 cut(s) 646, 1148
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 1044
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 170, 1082
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 263, 304, 714, 874
Bsc4I CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
Bse1I ACTGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 62, 306
BseDI CCNNGG 4 cut(s) 263, 304, 714, 874
BseGI GGATG 2 cut(s) 388, 439
BseLI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 4 cut(s) 503, 596, 663, 1035
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 674, 984
BseXI GCAGC 5 cut(s) 271, 529, 964, 986, 1081
BshFI GGCC 2 cut(s) 118, 132
BshNI GGYRCC 1 cut(s) 1049
BsiHKAI GWGCWC 2 cut(s) 749, 1098
BsiHKCI CYCGRG 2 cut(s) 269, 319
BslFI GGGAC 2 cut(s) 178, 336
BslI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
BsmAI GTCTC 3 cut(s) 846, 1019, 1114
BsmBI CGTCTC 1 cut(s) 846
BsmFI GGGAC 2 cut(s) 178, 336
BsmI GAATGC 3 cut(s) 364, 583, 1045
BsnI GGCC 2 cut(s) 118, 132
BsoBI CYCGRG 2 cut(s) 269, 319
Bsp1286I GDGCHC 2 cut(s) 749, 1098
Bsp143I GATC 3 cut(s) 43, 385, 912
Bsp1720I GCTNAGC 1 cut(s) 582
BspACI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
BspANI GGCC 2 cut(s) 118, 132
BspCNI CTCAG 4 cut(s) 502, 595, 664, 1036
BspLI GGNNCC 2 cut(s) 45, 1051
BspMAI CTGCAG 2 cut(s) 261, 735
BspPI GGATC 3 cut(s) 38, 51, 907
BspQI GCTCTTC 1 cut(s) 1080
BspT107I GGYRCC 1 cut(s) 1049
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 4 cut(s) 263, 304, 714, 874
BssMI GATC 3 cut(s) 43, 385, 912
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 2 cut(s) 714, 874
Bst2UI CCWGG 2 cut(s) 62, 306
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 3 cut(s) 119, 1017, 1080
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 628, 991
BstDEI CTNAG 5 cut(s) 489, 582, 672, 797, 1044
BstDSI CCRYGG 1 cut(s) 263
BstENI CCTNNNNNAGG 1 cut(s) 713
BstF5I GGATG 2 cut(s) 388, 439
BstKTI GATC 3 cut(s) 46, 388, 915
BstMAI GTCTC 3 cut(s) 846, 1019, 1114
BstMBI GATC 3 cut(s) 43, 385, 912
BstMWI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
BstNI CCWGG 2 cut(s) 62, 306
BstSCI CCNGG 2 cut(s) 60, 304
BstSFI CTRYAG 2 cut(s) 257, 731
BstV1I GCAGC 5 cut(s) 271, 529, 964, 986, 1081
BstV2I GAAGAC 2 cut(s) 646, 1148
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 2 cut(s) 118, 132
BtgI CCRYGG 1 cut(s) 263
BtrI CACGTC 1 cut(s) 856
BtsCI GGATG 2 cut(s) 388, 439
Cac8I GCNNGC 2 cut(s) 628, 991
Cfr13I GGNCC 1 cut(s) 878
CseI GACGC 2 cut(s) 54, 164
Csp6I GTAC 1 cut(s) 354
CviAII CATG 1 cut(s) 517
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 5 cut(s) 489, 582, 672, 797, 1044
DpnI GATC 3 cut(s) 45, 387, 914
DpnII GATC 3 cut(s) 43, 385, 912
EaeI YGGCCR 1 cut(s) 116
Eam1104I CTCTTC 3 cut(s) 119, 1017, 1080
EarI CTCTTC 3 cut(s) 119, 1017, 1080
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 747, 1096
Eco130I CCWWGG 2 cut(s) 714, 874
Eco24I GRGCYC 2 cut(s) 749, 1098
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 878
Eco53kI GAGCTC 2 cut(s) 747, 1096
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 269, 319
EcoICRI GAGCTC 2 cut(s) 747, 1096
EcoNI CCTNNNNNAGG 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 859
EcoRII CCWGG 2 cut(s) 60, 304
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 2 cut(s) 714, 874
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 749, 1098
ErhI CCWWGG 2 cut(s) 714, 874
Esp3I CGTCTC 1 cut(s) 846
FaeI CATG 1 cut(s) 520
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 2 cut(s) 178, 336
FatI CATG 1 cut(s) 516
Fnu4HI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
FokI GGATG 2 cut(s) 395, 446
FriOI GRGCYC 2 cut(s) 749, 1098
Fsp4HI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
FspBI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
GluI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 118, 132
HgaI GACGC 2 cut(s) 54, 164
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 9 cut(s) 50, 77, 220, 521, 665, 724, 815, 821, 963
HphI GGTGA 1 cut(s) 1094
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 5 cut(s) 55, 370, 820, 838, 968
Hpy188III TCNNGA 7 cut(s) 47, 187, 290, 321, 458, 656, 1099
Hpy8I GTNNAC 1 cut(s) 927
Hpy99I CGWCG 2 cut(s) 70, 158
HpyAV CCTTC 4 cut(s) 204, 236, 927, 1096
HpyCH4III ACNGT 1 cut(s) 706
HpyCH4IV ACGT 3 cut(s) 111, 768, 855
HpyCH4V TGCA 7 cut(s) 259, 464, 608, 626, 733, 993, 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
HpyF3I CTNAG 5 cut(s) 489, 582, 672, 797, 1044
HpySE526I ACGT 3 cut(s) 111, 768, 855
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 1 cut(s) 520
Kzo9I GATC 3 cut(s) 43, 385, 912
LguI GCTCTTC 1 cut(s) 1080
LmnI GCTCC 3 cut(s) 94, 658, 971
Lsp1109I GCAGC 5 cut(s) 271, 529, 964, 986, 1081
LweI GCATC 3 cut(s) 424, 451, 595
MaeI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
MaeII ACGT 3 cut(s) 111, 768, 855
MalI GATC 3 cut(s) 45, 387, 914
MboI GATC 3 cut(s) 43, 385, 912
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 749, 1098
MluCI AATT 8 cut(s) 15, 228, 424, 471, 596, 617, 859, 1138
MlyI GAGTC 2 cut(s) 59, 86
MmeI TCCRAC 2 cut(s) 21, 390
MnlI CCTC 8 cut(s) 122, 206, 264, 652, 655, 962, 1039, 1155
Mph1103I ATGCAT 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 2 cut(s) 149, 557
MspA1I CMGCKG 2 cut(s) 671, 977
MspR9I CCNGG 2 cut(s) 62, 306
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 3 cut(s) 364, 583, 1045
MvaI CCWGG 2 cut(s) 62, 306
MwoI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
NdeII GATC 3 cut(s) 43, 385, 912
NlaIII CATG 1 cut(s) 520
NlaIV GGNNCC 2 cut(s) 45, 1051
NsiI ATGCAT 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 1080
PctI GAATGC 3 cut(s) 364, 583, 1045
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 7 cut(s) 220, 521, 665, 724, 815, 821, 963
PkrI GCNGC 7 cut(s) 117, 134, 261, 544, 976, 979, 1071
PleI GAGTC 2 cut(s) 58, 85
PpsI GAGTC 2 cut(s) 58, 85
PsiI TTATAA 1 cut(s) 807
Psp124BI GAGCTC 2 cut(s) 749, 1098
Psp6I CCWGG 2 cut(s) 60, 304
PspGI CCWGG 2 cut(s) 60, 304
PspN4I GGNNCC 2 cut(s) 45, 1051
PspPI GGNCC 1 cut(s) 878
PspXI VCTCGAGB 1 cut(s) 269
PstI CTGCAG 2 cut(s) 261, 735
PsuI RGATCY 1 cut(s) 43
PvuII CAGCTG 2 cut(s) 671, 977
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SacI GAGCTC 2 cut(s) 749, 1098
SapI GCTCTTC 1 cut(s) 1080
SaqAI TTAA 2 cut(s) 149, 557
SatI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
Sau3AI GATC 3 cut(s) 43, 385, 912
Sau96I GGNCC 1 cut(s) 878
SchI GAGTC 2 cut(s) 59, 86
ScrFI CCNGG 2 cut(s) 62, 306
SduI GDGCHC 2 cut(s) 749, 1098
SfaNI GCATC 3 cut(s) 424, 451, 595
SfcI CTRYAG 2 cut(s) 257, 731
Sfr274I CTCGAG 1 cut(s) 269
SinI GGWCC 1 cut(s) 878
SlaI CTCGAG 1 cut(s) 269
SmlI CTYRAG 3 cut(s) 185, 269, 1097
SmoI CTYRAG 3 cut(s) 185, 269, 1097
SpeI ACTAGT 1 cut(s) 983
Sse9I AATT 8 cut(s) 15, 228, 424, 471, 596, 617, 859, 1138
SsiI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
SspI AATATT 1 cut(s) 555
SspMI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
SstI GAGCTC 2 cut(s) 749, 1098
StyD4I CCNGG 2 cut(s) 60, 304
StyI CCWWGG 2 cut(s) 714, 874
TaaI ACNGT 1 cut(s) 706
TaiI ACGT 3 cut(s) 114, 771, 858
TaqI TCGA 3 cut(s) 68, 270, 1008
TasI AATT 8 cut(s) 15, 228, 424, 471, 596, 617, 859, 1138
TauI GCSGC 2 cut(s) 118, 135
TfiI GAWTC 7 cut(s) 220, 521, 665, 724, 815, 821, 963
Tru1I TTAA 2 cut(s) 149, 557
Tru9I TTAA 2 cut(s) 149, 557
TseI GCWGC 5 cut(s) 259, 542, 974, 977, 1069
TspDTI ATGAA 5 cut(s) 255, 483, 564, 1026, 1052
TspGWI ACGGA 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 713
XapI RAATTY 2 cut(s) 617, 859
XbaI TCTAGA 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.