Rroxscaffold_3G00226830

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014861
Physical Location & Seq
Reverse (-)
10614533 .. 10619461
4929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226830.1

Sequence Viewer

Length: 1104 bp
ATGTCCGACGTTCTTCGGTTAAGGATAACCGGAGTGTTTGTGTTACGAGGCCAAGGTGATTGTAGCGATGTGGTTAGAGCTCTAACGTGTGGTCAAGATGGACCGGGGGATGTGTGGTCAAGATGGACCGGGGGACGTGTGGTCAAGATGGACCGGGGTTGTGTCATGTGGGAATTGAGTGTTGTCGACCCAAATCATGATTTTGCTGATTTGTCCCCTAAACTGCAGGACCTGAACTTTGTCAAAGCACATCCTGGTGATGAAGATATTGTTCCACTAATGGAGAAGTTACTTGAATCTCGTATTGAGCTTCACCCTCTTCTGATCACCTCCCACAGAAGACTAAAAGATATACTGTTCCTTGATCTTGCTTCGGCTTCAGCTGTCAAAACAACTATGGAAAGGGGACTCACCGATTTACATTTTTGTAACCCACCGGAGATCATGTTCATCATTTCCTTGGTGCTCGAAAATTTATGCCTGTCAACAGTTAACAGTGAAGATCTCATTTACTGCACCAAGGTAGATCCCTCTGTGATTCATGCTGACCTTTTCTCGACTGGTCTCAGTGCCTACCAGAAGAAGATCCAGCCCAGCGCAAAATATCTTGGTAATTTGCTTGGTGTTCAGAAGTTGCAAGTCACTGGGGAAGAGGAGATTCCAGATGGAGTAGTTGCTGTCCTGACACCTGATACACCAGATGTTCTATCTCATGTCTCAACAAGAGCAAGAAATAGCAAGGTTGGTGCAAAATCTTGCAACATAAGATTTTTGAGAGGAAGGGTGCCAGCATGGATTAAAATTCCCGTATCAGTTGCCATACCATTTGGAGTCTTTGAAAAGGTCCTATTAGAAGATATTCAAATAAATCTTGAAAGAGTCCCTAAGTTACCCAAAACCGAGAAATCTGGTTCTGATTTTCATCAGGCCTTTGAGTATTCTTACCTCATATGTTATCCCGTCTTCAGGTCCATCTCTGAATCTAGAAAGAGAAAGAAAGCTGAAAGGGAAGTGTTGAAGGAAGCTGATGTGGTGATGGACCTCAGATTGTTAATCTTTTCTCTGAATCTTGGTGTTATGTTGCTGTTGCTGCCTCAGGAGTAG

Protein Analysis

367

Amino Acids

41.06

Weight (kDa)

6.28

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 213 - 254 3.3e-16 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 784
AccI GTMKAC 1 cut(s) 186
AclWI GGATC 2 cut(s) 521, 580
AcsI RAATTY 2 cut(s) 472, 801
AcuI CTGAAG 2 cut(s) 363, 949
AfiI CCNNNNNNNGG 1 cut(s) 966
AflIII ACRYGT 2 cut(s) 86, 136
AgsI TTSAA 5 cut(s) 296, 839, 863, 875, 1018
AhdI GACNNNNNGTC 1 cut(s) 140
AjiI CACGTC 1 cut(s) 137
AjnI CCWGG 1 cut(s) 253
AloI GAACNNNNNNTCC 2 cut(s) 431, 463
AluBI AGCT 5 cut(s) 80, 310, 383, 1001, 1025
AluI AGCT 5 cut(s) 80, 310, 383, 1001, 1025
Alw21I GWGCWC 2 cut(s) 82, 468
Alw26I GTCTC 2 cut(s) 569, 721
AlwI GGATC 2 cut(s) 521, 580
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 2 cut(s) 49, 927
ApeKI GCWGC 1 cut(s) 1090
ApoI RAATTY 2 cut(s) 472, 801
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 1 cut(s) 858
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
AsuC2I CCSGG 3 cut(s) 105, 130, 155
AsuHPI GGTGA 6 cut(s) 68, 269, 305, 319, 403, 1045
AvaII GGWCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
AxyI CCTNAGG 1 cut(s) 1095
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 2 cut(s) 346, 955
Bbv12I GWGCWC 2 cut(s) 82, 468
BbvI GCAGC 1 cut(s) 1077
BccI CCATC 6 cut(s) 92, 117, 142, 659, 980, 1030
BciT130I CCWGG 1 cut(s) 255
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 3 cut(s) 105, 130, 155
BcoDI GTCTC 2 cut(s) 569, 721
BfaI CTAG 1 cut(s) 984
BfmI CTRYAG 1 cut(s) 224
BglII AGATCT 1 cut(s) 502
BisI GCNGC 1 cut(s) 1091
BlsI GCNGC 1 cut(s) 1092
Bme1390I CCNGG 4 cut(s) 105, 130, 155, 255
Bme18I GGWCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
BmeRI GACNNNNNGTC 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 137
BmgT120I GGNCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
BmiI GGNNCC 1 cut(s) 786
BmrFI CCNGG 4 cut(s) 105, 130, 155, 255
BmrI ACTGGG 1 cut(s) 654
BmuI ACTGGG 1 cut(s) 654
BpiI GAAGAC 2 cut(s) 346, 955
BpuMI CCSGG 3 cut(s) 105, 130, 155
BsaBI GATNNNNATC 1 cut(s) 921
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 6 cut(s) 52, 104, 129, 154, 459, 519
BsaWI WCCGGW 2 cut(s) 29, 436
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 431, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 966
Bse1I ACTGG 2 cut(s) 565, 649
Bse21I CCTNAGG 1 cut(s) 1095
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 6 cut(s) 52, 104, 129, 154, 459, 519
BseGI GGATG 2 cut(s) 115, 250
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 1 cut(s) 966
BseMII CTCAG 2 cut(s) 580, 1057
BseNI ACTGG 2 cut(s) 565, 649
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 1 cut(s) 1077
BseYI CCCAGC 1 cut(s) 593
BsgI GTGCAG 1 cut(s) 499
BshFI GGCC 2 cut(s) 51, 929
BshNI GGYRCC 1 cut(s) 784
BsiHKAI GWGCWC 2 cut(s) 82, 468
BsiSI CCGG 5 cut(s) 30, 104, 129, 154, 437
BslFI GGGAC 4 cut(s) 147, 199, 420, 866
BslI CCNNNNNNNGG 1 cut(s) 966
BsmAI GTCTC 2 cut(s) 569, 721
BsmFI GGGAC 4 cut(s) 147, 199, 420, 866
BsnI GGCC 2 cut(s) 51, 929
Bso31I GGTCTC 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 82, 468
Bsp143I GATC 6 cut(s) 324, 364, 441, 502, 526, 585
BspANI GGCC 2 cut(s) 51, 929
BspCNI CTCAG 2 cut(s) 579, 1056
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 786
BspMAI CTGCAG 1 cut(s) 228
BspPI GGATC 2 cut(s) 521, 580
BspT107I GGYRCC 1 cut(s) 784
BspTNI GGTCTC 1 cut(s) 569
BsrI ACTGG 2 cut(s) 565, 649
BssECI CCNNGG 6 cut(s) 52, 104, 129, 154, 459, 519
BssMI GATC 6 cut(s) 324, 364, 441, 502, 526, 585
BssT1I CCWWGG 3 cut(s) 52, 459, 519
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 3 cut(s) 357, 490, 497
Bst6I CTCTTC 2 cut(s) 324, 645
BstC8I GCNNGC 1 cut(s) 789
BstDEI CTNAG 4 cut(s) 566, 885, 1043, 1095
BstF5I GGATG 2 cut(s) 115, 250
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 6 cut(s) 327, 367, 444, 505, 529, 588
BstMAI GTCTC 2 cut(s) 569, 721
BstMBI GATC 6 cut(s) 324, 364, 441, 502, 526, 585
BstMWI GCNNNNNNNGC 1 cut(s) 1090
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 4 cut(s) 103, 128, 153, 253
BstSFI CTRYAG 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 1077
BstV2I GAAGAC 2 cut(s) 346, 955
BstX2I RGATCY 3 cut(s) 502, 526, 585
BstYI RGATCY 3 cut(s) 502, 526, 585
Bsu36I CCTNAGG 1 cut(s) 1095
BsuRI GGCC 2 cut(s) 51, 929
BtgZI GCGATG 1 cut(s) 81
BtrI CACGTC 1 cut(s) 137
BtsCI GGATG 2 cut(s) 115, 250
BtsIMutI CAGTG 3 cut(s) 502, 574, 642
Cac8I GCNNGC 1 cut(s) 789
CaiI CAGNNNCTG 1 cut(s) 232
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 599
Cfr13I GGNCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
CviAII CATG 6 cut(s) 166, 197, 445, 542, 713, 792
CviJI RGCY 9 cut(s) 51, 80, 310, 377, 383, 592, 929, 1001, 1025
CviKI_1 RGCY 9 cut(s) 51, 80, 310, 377, 383, 592, 929, 1001, 1025
DdeI CTNAG 4 cut(s) 566, 885, 1043, 1095
DpnI GATC 6 cut(s) 326, 366, 443, 504, 528, 587
DpnII GATC 6 cut(s) 324, 364, 441, 502, 526, 585
DriI GACNNNNNGTC 1 cut(s) 140
Eam1104I CTCTTC 2 cut(s) 324, 645
Eam1105I GACNNNNNGTC 1 cut(s) 140
EarI CTCTTC 2 cut(s) 324, 645
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 3 cut(s) 52, 459, 519
Eco147I AGGCCT 1 cut(s) 929
Eco24I GRGCYC 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 2 cut(s) 363, 949
Eco81I CCTNAGG 1 cut(s) 1095
EcoICRI GAGCTC 1 cut(s) 80
EcoO109I RGGNCCY 2 cut(s) 229, 844
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 3 cut(s) 52, 459, 519
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 3 cut(s) 52, 459, 519
FaeI CATG 6 cut(s) 169, 200, 448, 545, 716, 795
FaqI GGGAC 4 cut(s) 147, 199, 420, 866
FatI CATG 6 cut(s) 165, 196, 444, 541, 712, 791
FauNDI CATATG 1 cut(s) 950
FbaI TGATCA 1 cut(s) 324
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 1091
FokI GGATG 2 cut(s) 122, 237
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 1 cut(s) 1091
FspBI CTAG 1 cut(s) 984
GlaI GCGC 1 cut(s) 598
GluI GCNGC 1 cut(s) 1091
GsaI CCCAGC 1 cut(s) 597
HaeIII GGCC 2 cut(s) 51, 929
HapII CCGG 5 cut(s) 30, 104, 129, 154, 437
HhaI GCGC 1 cut(s) 599
Hin1II CATG 6 cut(s) 169, 200, 448, 545, 716, 795
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HincII GTYRAC 3 cut(s) 187, 486, 493
HindII GTYRAC 3 cut(s) 187, 486, 493
HinfI GANTC 8 cut(s) 296, 408, 538, 658, 831, 879, 980, 1066
HpaI GTTAAC 1 cut(s) 493
HpaII CCGG 5 cut(s) 30, 104, 129, 154, 437
HphI GGTGA 6 cut(s) 68, 269, 305, 319, 403, 1045
Hpy166II GTNNAC 3 cut(s) 187, 486, 493
Hpy188I TCNGA 7 cut(s) 7, 324, 630, 916, 979, 1046, 1065
Hpy8I GTNNAC 3 cut(s) 187, 486, 493
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 2 cut(s) 774, 1012
HpyCH4III ACNGT 3 cut(s) 357, 490, 497
HpyCH4IV ACGT 3 cut(s) 9, 86, 136
HpyCH4V TGCA 5 cut(s) 226, 516, 637, 749, 759
HpyF10VI GCNNNNNNNGC 1 cut(s) 1090
HpyF3I CTNAG 4 cut(s) 566, 885, 1043, 1095
HpySE526I ACGT 3 cut(s) 9, 86, 136
Hsp92II CATG 6 cut(s) 169, 200, 448, 545, 716, 795
HspAI GCGC 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 324
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 6 cut(s) 324, 364, 441, 502, 526, 585
Lsp1109I GCAGC 1 cut(s) 1077
MaeI CTAG 1 cut(s) 984
MaeII ACGT 3 cut(s) 9, 86, 136
MaeIII GTNAC 5 cut(s) 42, 288, 428, 640, 888
MalI GATC 6 cut(s) 326, 366, 443, 504, 528, 587
MboI GATC 6 cut(s) 324, 364, 441, 502, 526, 585
MflI RGATCY 3 cut(s) 502, 526, 585
MhlI GDGCHC 2 cut(s) 82, 468
MluCI AATT 4 cut(s) 173, 472, 613, 801
MlyI GAGTC 3 cut(s) 402, 840, 888
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 9 cut(s) 41, 327, 340, 541, 646, 770, 956, 1052, 1104
MroXI GAANNNNTTC 1 cut(s) 858
MseI TTAA 4 cut(s) 20, 492, 798, 1052
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 5 cut(s) 30, 104, 129, 154, 437
MspR9I CCNGG 4 cut(s) 105, 130, 155, 255
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 1090
NciI CCSGG 3 cut(s) 105, 130, 155
NdeI CATATG 1 cut(s) 950
NdeII GATC 6 cut(s) 324, 364, 441, 502, 526, 585
NlaIII CATG 6 cut(s) 169, 200, 448, 545, 716, 795
NlaIV GGNNCC 1 cut(s) 786
NmuCI GTSAC 1 cut(s) 640
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 929
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 5 cut(s) 296, 538, 658, 980, 1066
PkrI GCNGC 1 cut(s) 1092
PleI GAGTC 3 cut(s) 402, 839, 887
PpsI GAGTC 3 cut(s) 402, 839, 887
PpuMI RGGWCCY 2 cut(s) 229, 844
Psp124BI GAGCTC 1 cut(s) 82
Psp5II RGGWCCY 2 cut(s) 229, 844
Psp6I CCWGG 1 cut(s) 253
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 786
PspPI GGNCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
PspPPI RGGWCCY 2 cut(s) 229, 844
PstI CTGCAG 1 cut(s) 228
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 3 cut(s) 502, 526, 585
PvuII CAGCTG 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 82
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 4 cut(s) 20, 492, 798, 1052
SatI GCNGC 1 cut(s) 1091
Sau3AI GATC 6 cut(s) 324, 364, 441, 502, 526, 585
Sau96I GGNCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
SchI GAGTC 3 cut(s) 402, 840, 888
ScrFI CCNGG 4 cut(s) 105, 130, 155, 255
SduI GDGCHC 2 cut(s) 82, 468
SfcI CTRYAG 1 cut(s) 224
SinI GGWCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 4 cut(s) 173, 472, 613, 801
SseBI AGGCCT 1 cut(s) 929
SspMI CTAG 1 cut(s) 984
SstI GAGCTC 1 cut(s) 82
StuI AGGCCT 1 cut(s) 929
StyD4I CCNGG 4 cut(s) 103, 128, 153, 253
StyI CCWWGG 3 cut(s) 52, 459, 519
TaaI ACNGT 3 cut(s) 357, 490, 497
TaiI ACGT 3 cut(s) 12, 89, 139
TaqI TCGA 3 cut(s) 186, 468, 557
TasI AATT 4 cut(s) 173, 472, 613, 801
TfiI GAWTC 5 cut(s) 296, 538, 658, 980, 1066
Tru1I TTAA 4 cut(s) 20, 492, 798, 1052
Tru9I TTAA 4 cut(s) 20, 492, 798, 1052
TscAI CASTG 3 cut(s) 502, 574, 649
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 1 cut(s) 1090
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 4 cut(s) 276, 439, 530, 911
TspRI CASTG 3 cut(s) 502, 574, 649
VpaK11BI GGWCC 7 cut(s) 101, 126, 151, 229, 844, 969, 1039
XapI RAATTY 2 cut(s) 472, 801
XbaI TCTAGA 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.