Rroxscaffold_3G00227310

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014861
Physical Location & Seq
Forward (+)
11423666 .. 11433478
9813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227310.1

Sequence Viewer

Length: 2163 bp
ATGGCGACTTCCTTTACCACATGTATTCACCCTCTCAATTCATCTATACAAACAGATTTGATGGCAACGGTGGCTGCTAATTTGCTCCCATTCAAGTGGAAGCATGCTACTCAAATTGGCTCCGTCCCCAAGCTGCACGCACTCGCACTCAACCAAATCTTAATCACCACCAACCCTGCTGTTCATTCAGGTGATGACCTTGAATCTGCTGCTGAAGTTTTGGTTCCCTCAAATAGGAAGTTACTAGAATCTCGTATTGAGCGTCGCCCTCTTCTGATCATCTTCCACAGAAGATTAAAAGATATGCTGTTCCTTGATCTTGCTTTGGCTTCAGCTGTTGAAACAACATGGGAAAGGGGACTCACAGATCTACATTTTTCTAACCCACCGGACTGGTACCGTATTAGTGAACTGTATAAACCCAATAATGGTCAGTCGGCATTAAAATCAAAGGCTATTCTTGATTGCTTACAATTAGTACTTGCTGATAGGTCTCAGTGCTACCAAAAGAAGATCCAGCCCAGTGCAAAATATCTTGGTAATGTGCTTGGTGTTCAGAAGCCAGCGATTGATATTTTTCCTGAGGAGTTACTACGGGATGGATCAGAGACTATTCTCTCTACTCTCATCAATCGCTTTGATCCCGTTCTTAGAAAAGTTGGTGCAAAATCTTGCAACATAAGATCTTTGAGAGGAATGGTGCCAGCATGGATTAAAATTCCCATGTCAGTTGCCATACCATTTGGAGCCTTTGATAAGGTCATATTAGAAGATGTTAATAATGAAATATTGTGTGTTAAACCTGAAGAGATAGAAGAGAAGGCATTTGTAGTTGAGGAGAACTATGAGGTAATACATGCAGAAGGATATGGTTCTACTGCGAATTATAACTCAAGTTATGGCTCTCAAATGCCTGGAAGTACAATTCATATGGTGGAAGTGGAGGATTATATGGTCGGGGGATGTACAGCAAGTCACAACAGCATGTATGCTGGAGGTTCTGGTGCGCTCTACGGGGACTACATTGAGGGACCGAAGGCCACTTCTACAGGATCTTGCTTAAAGAAGATACATTGGCTCTATTTTTTTGTGCAGAAGCTTCCAAGAAAGTATGTGAAGGACCATGGAGATGAGCTATCAAATCCTGCACATCTTAAGTTTCGCGGTAGAGAATGGGCAATGAAACTTAAAGATACAAAGATGAGGCTTGGTTTGACAAGGGTTGGGAAGAGTTCTCAAACTTTTACTCTTTTGAGTACAATGACCTCCTCATTTTTGGATATGAAGGCCATTCAACATTCCAGAACACCGAAGTTGATAGAAACTGATGCAGATGATTTTCAACCAAATCCTCTGCTTCATAGCAGAACTAGGACAAGTTCAAGTGGTGGTAAAGCAGAAGTACAAGAGGAGGCTCCAAGTCTTGAGAAATCACAAATGAAAACCAAAGTTACCCATAGGTCCAGATCAGAATTGAAAAGGAAACAAGATTGTTATGGGAAAAAGACCGAGGGAGGCAAGTCTAACAATCAAAGGTTCCAAAGAACATCCGAGGCTATGCAGAGAGTCAAATCTGAATATCAGTTTTTCAGAATTGAAGTGCAGGCCACTGATATCCATCGAATGATATTGTGCTTGCCATTCGAGTTTATGAGGACACATCTTGTTAATGAATCTGGTGAGGTTACCCTTCAGCTTTCAAATGGAAGAATTTGTCAAGTTGAATTGAAATGTGAAAACTCAAGAGCCGAATTCCAGTCTGGTTGGTGGGATTTTATGCAAGACAACCATTTAAAAGTTGGTGATGTATGTGTTTTTATGTTGATTGACAATGCTAGACTTCTATTTGAAGTTGTCATTTTCCACAAGGACGGCACCATAGAACCTTTTGTGATCATTGATGCTGATAGAGTCTCATTTGAAGTAGTCATAGAAGCTGGGAATACCACGTTATCCTTAGGTCAGTTATTTATTATTTTGGAATTGTTATTTGATGGAAGTGCCAAAAAGACTAACTATGCCATTGTTCCTTCCATTATAACTACAACTGCAGACAATGATGATGATGAAAATGAAGATTCAGAATTGGTTGAATCCTCATTAGCACAACGGCCTCACAAGAGAAAGAGAACAGGTTGTAATGTACCAACTTCGTATGGATGA

Protein Analysis

720

Amino Acids

80.98

Weight (kDa)

6.86

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 529 - 622 9.1e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 888, 2039
Acc65I GGTACC 1 cut(s) 396
AccB1I GGYRCC 3 cut(s) 396, 700, 1874
AccII CGCG 1 cut(s) 1164
AciI CCGC 1 cut(s) 1164
AclWI GGATC 4 cut(s) 508, 610, 635, 1060
AcsI RAATTY 3 cut(s) 717, 1710, 1751
AcuI CTGAAG 4 cut(s) 234, 315, 825, 1676
AfaI GTAC 7 cut(s) 398, 480, 922, 967, 1258, 1404, 2145
AfiI CCNNNNNNNGG 2 cut(s) 234, 428
AflII CTTAAG 1 cut(s) 1154
AflIII ACRYGT 1 cut(s) 20
AjnI CCWGG 1 cut(s) 913
AluBI AGCT 6 cut(s) 133, 335, 1099, 1135, 1696, 1937
AluI AGCT 6 cut(s) 133, 335, 1099, 1135, 1696, 1937
Alw26I GTCTC 3 cut(s) 498, 602, 1918
AlwI GGATC 4 cut(s) 508, 610, 635, 1060
AoxI GGCC 4 cut(s) 1038, 1287, 1605, 2111
ApeKI GCWGC 3 cut(s) 74, 133, 209
ApoI RAATTY 3 cut(s) 717, 1710, 1751
Asp718I GGTACC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 1009
AspS9I GGNCC 3 cut(s) 1031, 1120, 1461
AsuHPI GGTGA 5 cut(s) 20, 157, 203, 1691, 1814
AvaII GGWCC 3 cut(s) 1031, 1120, 1461
AxyI CCTNAGG 2 cut(s) 582, 1957
BanI GGYRCC 3 cut(s) 396, 700, 1874
BbvI GCAGC 3 cut(s) 61, 120, 196
BccI CCATC 4 cut(s) 55, 593, 1626, 1988
BceAI ACGGC 2 cut(s) 1888, 2126
BciT130I CCWGG 1 cut(s) 915
BclI TGATCA 2 cut(s) 276, 1893
BcoDI GTCTC 3 cut(s) 498, 602, 1918
BfaI CTAG 3 cut(s) 245, 1371, 1836
BfmI CTRYAG 2 cut(s) 1047, 2049
BfrI CTTAAG 1 cut(s) 1154
BglII AGATCT 2 cut(s) 367, 683
BisI GCNGC 3 cut(s) 75, 134, 210
BlsI GCNGC 3 cut(s) 76, 135, 211
BmcAI AGTACT 1 cut(s) 480
Bme1390I CCNGG 1 cut(s) 915
Bme18I GGWCC 3 cut(s) 1031, 1120, 1461
BmgT120I GGNCC 3 cut(s) 1031, 1120, 1461
BmiI GGNNCC 9 cut(s) 121, 225, 398, 702, 748, 1032, 1416, 1538, 1876
BmrFI CCNGG 1 cut(s) 915
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 2 cut(s) 1318, 1891
BmuI ACTGGG 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 1014
BpuEI CTTGAG 3 cut(s) 877, 1445, 1726
BsaBI GATNNNNATC 1 cut(s) 1617
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 3 cut(s) 1123, 1509, 1551
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 428
Bse1I ACTGG 3 cut(s) 398, 522, 1756
Bse21I CCTNAGG 2 cut(s) 582, 1957
Bse3DI GCAATG 1 cut(s) 1185
Bse8I GATNNNNATC 1 cut(s) 1617
BseBI CCWGG 1 cut(s) 915
BseDI CCNNGG 3 cut(s) 1123, 1509, 1551
BseGI GGATG 3 cut(s) 604, 968, 1547
BseJI GATNNNNATC 1 cut(s) 1617
BseLI CCNNNNNNNGG 2 cut(s) 234, 428
BseMI GCAATG 1 cut(s) 1185
BseMII CTCAG 2 cut(s) 509, 573
BseNI ACTGG 3 cut(s) 398, 522, 1756
BseRI GAGGAG 4 cut(s) 599, 851, 1258, 1424
BseXI GCAGC 3 cut(s) 61, 120, 196
BseYI CCCAGC 1 cut(s) 1937
BsgI GTGCAG 4 cut(s) 119, 1112, 1131, 1622
Bsh1236I CGCG 1 cut(s) 1164
BshFI GGCC 4 cut(s) 1040, 1289, 1607, 2113
BshNI GGYRCC 3 cut(s) 396, 700, 1874
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 4 cut(s) 110, 372, 1031, 1044
BslI CCNNNNNNNGG 2 cut(s) 234, 428
BsmAI GTCTC 3 cut(s) 498, 602, 1918
BsmFI GGGAC 4 cut(s) 110, 372, 1031, 1044
BsnI GGCC 4 cut(s) 1040, 1289, 1607, 2113
Bso31I GGTCTC 1 cut(s) 498
Bsp1407I TGTACA 1 cut(s) 965
Bsp19I CCATGG 1 cut(s) 1123
BspACI CCGC 1 cut(s) 1164
BspANI GGCC 4 cut(s) 1040, 1289, 1607, 2113
BspCNI CTCAG 2 cut(s) 508, 574
BspFNI CGCG 1 cut(s) 1164
BspLI GGNNCC 9 cut(s) 121, 225, 398, 702, 748, 1032, 1416, 1538, 1876
BspMAI CTGCAG 1 cut(s) 2053
BspPI GGATC 4 cut(s) 508, 610, 635, 1060
BspT107I GGYRCC 3 cut(s) 396, 700, 1874
BspTI CTTAAG 1 cut(s) 1154
BspTNI GGTCTC 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 1185
BsrGI TGTACA 1 cut(s) 965
BsrI ACTGG 3 cut(s) 398, 522, 1756
BssECI CCNNGG 3 cut(s) 1123, 1509, 1551
BssT1I CCWWGG 1 cut(s) 1123
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 3 cut(s) 70, 401, 414
Bst6I CTCTTC 4 cut(s) 276, 801, 810, 1223
BstAFI CTTAAG 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 965
BstC8I GCNNGC 6 cut(s) 105, 138, 564, 705, 1605, 1637
BstDEI CTNAG 4 cut(s) 495, 582, 650, 1957
BstDSI CCRYGG 1 cut(s) 1123
BstEII GGTNACC 1 cut(s) 1684
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 3 cut(s) 604, 968, 1547
BstFNI CGCG 1 cut(s) 1164
BstHHI GCGC 1 cut(s) 1009
BstMAI GTCTC 3 cut(s) 498, 602, 1918
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 915
BstNSI RCATGY 4 cut(s) 24, 107, 860, 988
BstPI GGTNACC 1 cut(s) 1684
BstSCI CCNGG 1 cut(s) 913
BstSFI CTRYAG 2 cut(s) 1047, 2049
BstUI CGCG 1 cut(s) 1164
BstV1I GCAGC 3 cut(s) 61, 120, 196
BstX2I RGATCY 4 cut(s) 367, 513, 683, 1052
BstXI CCANNNNNNTGG 2 cut(s) 96, 393
BstYI RGATCY 4 cut(s) 367, 513, 683, 1052
Bsu36I CCTNAGG 2 cut(s) 582, 1957
BsuRI GGCC 4 cut(s) 1040, 1289, 1607, 2113
BtgI CCRYGG 1 cut(s) 1123
BtsCI GGATG 3 cut(s) 604, 968, 1547
BtsIMutI CAGTG 3 cut(s) 503, 529, 1608
Cac8I GCNNGC 6 cut(s) 105, 138, 564, 705, 1605, 1637
CfoI GCGC 1 cut(s) 1009
Cfr13I GGNCC 3 cut(s) 1031, 1120, 1461
CseI GACGC 1 cut(s) 251
Csp6I GTAC 7 cut(s) 397, 479, 921, 966, 1257, 1403, 2144
CviAII CATG 8 cut(s) 21, 104, 348, 708, 724, 857, 985, 1124
CviQI GTAC 7 cut(s) 397, 479, 921, 966, 1257, 1403, 2144
DdeI CTNAG 4 cut(s) 495, 582, 650, 1957
DraI TTTAAA 1 cut(s) 1794
Eam1104I CTCTTC 4 cut(s) 276, 801, 810, 1223
EarI CTCTTC 4 cut(s) 276, 801, 810, 1223
Eco130I CCWWGG 1 cut(s) 1123
Eco31I GGTCTC 1 cut(s) 498
Eco32I GATATC 1 cut(s) 1615
Eco47I GGWCC 3 cut(s) 1031, 1120, 1461
Eco57I CTGAAG 4 cut(s) 234, 315, 825, 1676
Eco81I CCTNAGG 2 cut(s) 582, 1957
Eco91I GGTNACC 1 cut(s) 1684
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoO65I GGTNACC 1 cut(s) 1684
EcoRI GAATTC 1 cut(s) 1751
EcoRII CCWGG 1 cut(s) 913
EcoRV GATATC 1 cut(s) 1615
EcoT14I CCWWGG 1 cut(s) 1123
ErhI CCWWGG 1 cut(s) 1123
FaeI CATG 8 cut(s) 24, 107, 351, 711, 727, 860, 988, 1127
FaqI GGGAC 4 cut(s) 110, 372, 1031, 1044
FatI CATG 8 cut(s) 20, 103, 347, 707, 723, 856, 984, 1123
FauNDI CATATG 1 cut(s) 930
FbaI TGATCA 2 cut(s) 276, 1893
Fnu4HI GCNGC 3 cut(s) 75, 134, 210
FokI GGATG 3 cut(s) 611, 975, 1534
Fsp4HI GCNGC 3 cut(s) 75, 134, 210
FspBI CTAG 3 cut(s) 245, 1371, 1836
GlaI GCGC 1 cut(s) 1008
GluI GCNGC 3 cut(s) 75, 134, 210
GsaI CCCAGC 1 cut(s) 1941
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 4 cut(s) 1040, 1289, 1607, 2113
HapII CCGG 1 cut(s) 389
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 1009
Hin1II CATG 8 cut(s) 24, 107, 351, 711, 727, 860, 988, 1127
Hin6I GCGC 1 cut(s) 1007
HinP1I GCGC 1 cut(s) 1007
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 8 cut(s) 203, 248, 360, 1566, 1673, 1911, 2078, 2093
HpaII CCGG 1 cut(s) 389
HphI GGTGA 5 cut(s) 20, 157, 203, 1691, 1814
Hpy166II GTNNAC 1 cut(s) 410
Hpy188I TCNGA 8 cut(s) 276, 558, 607, 1471, 1552, 1576, 1592, 2083
Hpy188III TCNNGA 6 cut(s) 461, 581, 1302, 1424, 1464, 1743
Hpy8I GTNNAC 1 cut(s) 410
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 7 cut(s) 814, 857, 1030, 1111, 1279, 1700, 2040
HpyCH4III ACNGT 3 cut(s) 70, 401, 414
HpyCH4IV ACGT 1 cut(s) 1949
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 4 cut(s) 495, 582, 650, 1957
HpySE526I ACGT 1 cut(s) 1949
Hsp92II CATG 8 cut(s) 24, 107, 351, 711, 727, 860, 988, 1127
HspAI GCGC 1 cut(s) 1007
KpnI GGTACC 1 cut(s) 400
Ksp22I TGATCA 2 cut(s) 276, 1893
LmnI GCTCC 4 cut(s) 90, 125, 746, 1420
Lsp1109I GCAGC 3 cut(s) 61, 120, 196
LweI GCATC 2 cut(s) 1318, 1891
MaeI CTAG 3 cut(s) 245, 1371, 1836
MaeII ACGT 1 cut(s) 1949
MaeIII GTNAC 5 cut(s) 240, 588, 974, 1450, 1684
MflI RGATCY 4 cut(s) 367, 513, 683, 1052
MlyI GAGTC 3 cut(s) 354, 1575, 1920
MslI CAYNNNNRTG 3 cut(s) 94, 189, 1128
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 1154
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 915
MvaI CCWGG 1 cut(s) 915
MvnI CGCG 1 cut(s) 1164
MwoI GCNNNNNNNGC 1 cut(s) 71
NcoI CCATGG 1 cut(s) 1123
NdeI CATATG 1 cut(s) 930
NlaIII CATG 8 cut(s) 24, 107, 351, 711, 727, 860, 988, 1127
NlaIV GGNNCC 9 cut(s) 121, 225, 398, 702, 748, 1032, 1416, 1538, 1876
NmuCI GTSAC 1 cut(s) 974
NspI RCATGY 4 cut(s) 24, 107, 860, 988
PaeI GCATGC 1 cut(s) 107
PciI ACATGT 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 5 cut(s) 203, 248, 1673, 2078, 2093
PkrI GCNGC 3 cut(s) 76, 135, 211
PleI GAGTC 3 cut(s) 354, 1574, 1919
PpsI GAGTC 3 cut(s) 354, 1574, 1919
PscI ACATGT 1 cut(s) 20
PsiI TTATAA 2 cut(s) 888, 2039
Psp6I CCWGG 1 cut(s) 913
PspEI GGTNACC 1 cut(s) 1684
PspFI CCCAGC 1 cut(s) 1937
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 9 cut(s) 121, 225, 398, 702, 748, 1032, 1416, 1538, 1876
PspPI GGNCC 3 cut(s) 1031, 1120, 1461
PstI CTGCAG 1 cut(s) 2053
PsuI RGATCY 4 cut(s) 367, 513, 683, 1052
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 7 cut(s) 398, 480, 922, 967, 1258, 1404, 2145
RsaNI GTAC 7 cut(s) 397, 479, 921, 966, 1257, 1403, 2144
RseI CAYNNNNRTG 3 cut(s) 94, 189, 1128
SatI GCNGC 3 cut(s) 75, 134, 210
Sau96I GGNCC 3 cut(s) 1031, 1120, 1461
ScaI AGTACT 1 cut(s) 480
SchI GAGTC 3 cut(s) 354, 1575, 1920
ScrFI CCNGG 1 cut(s) 915
SfaNI GCATC 2 cut(s) 1318, 1891
SfcI CTRYAG 2 cut(s) 1047, 2049
SinI GGWCC 3 cut(s) 1031, 1120, 1461
SmiMI CAYNNNNRTG 3 cut(s) 94, 189, 1128
SmlI CTYRAG 4 cut(s) 892, 1154, 1424, 1741
SmoI CTYRAG 4 cut(s) 892, 1154, 1424, 1741
SphI GCATGC 1 cut(s) 107
SsiI CCGC 1 cut(s) 1164
SspI AATATT 1 cut(s) 789
SspMI CTAG 3 cut(s) 245, 1371, 1836
StyD4I CCNGG 1 cut(s) 913
StyI CCWWGG 1 cut(s) 1123
TaaI ACNGT 3 cut(s) 70, 401, 414
TaiI ACGT 1 cut(s) 1952
TaqI TCGA 2 cut(s) 1621, 1644
TaqII GACCGA 2 cut(s) 1048, 1523
TatI WGTACW 5 cut(s) 478, 920, 965, 1256, 1402
TfiI GAWTC 5 cut(s) 203, 248, 1673, 2078, 2093
TscAI CASTG 3 cut(s) 503, 529, 1615
TseFI GTSAC 1 cut(s) 974
TseI GCWGC 3 cut(s) 74, 133, 209
Tsp45I GTSAC 1 cut(s) 974
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 3 cut(s) 503, 529, 1615
Vha464I CTTAAG 1 cut(s) 1154
VpaK11BI GGWCC 3 cut(s) 1031, 1120, 1461
XagI CCTNNNNNAGG 1 cut(s) 232
XapI RAATTY 3 cut(s) 717, 1710, 1751
XceI RCATGY 4 cut(s) 24, 107, 860, 988
XcmI CCANNNNNNNNNTGG 1 cut(s) 1796
XspI CTAG 3 cut(s) 245, 1371, 1836
ZrmI AGTACT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.