Rroxscaffold_3G00241340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002100
Physical Location & Seq
Reverse (-)
33601120 .. 33603904
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00241340.1

Sequence Viewer

Length: 660 bp
ATGGCAGGCAGAGAAGTTCGAGAATACACCAATCTCACAGACCCTAAAGATAAAAAATGGGGGAAAGGGAAGGATAAGGTGGACGATGAAGACGTCACCTTCCAACGCATGGTTGCAAAGTTTGAGTATACAGCCTTGGACAGTGATGACCTATTATATCTCAAGGAGCAGATGGAAGCTGAGGAGGATGCAGAACGCCTCCTTCGTCGCACTGAGAAGCGAGCATTTGCTGCATTTAAGATATCCTTTTATGAAATTGCTTCTAGTTCTCTTTCTTCTCCCTCTTCTATCTTAGTTTACTTAACCAATGCACACCAAGCTGCAAGTCTAGCAGATTCTTCACCTGCATCGGCTCCCTTGACACTTCGTGTTGAGCCCAAACAAAAGAGTGGGATTAGACAGCAAGATCTACTGAAAAAGGTGGTAGAAGTTAAACCCAAAAAGCAGAAAGTTGCAAGTCCATCTAATGCTAATCGGTTGCCCCTCTCCCAAAAGAATGTTGGTGCTTCTTCGACAAATCTTAAGCCTGAATCTGACCAGGACAGGGACAAGGTGCATTTGTCATCACCATCTTCCAAAATAGATGGTGAGTCCAAGGTGGAAACGACTGCCCAAAGCTTATTGGGCTTAGCATATGCAAGCTCTGACGATGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.26

Weight (kDa)

5.87

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
AatII GACGTC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 109
AccI GTMKAC 1 cut(s) 128
AcyI GRCGYC 1 cut(s) 93
AdeI CACNNNGTG 1 cut(s) 368
AfiI CCNNNNNNNGG 2 cut(s) 109, 544
AflII CTTAAG 1 cut(s) 521
AjnI CCWGG 1 cut(s) 537
AluBI AGCT 4 cut(s) 179, 320, 618, 642
AluI AGCT 4 cut(s) 179, 320, 618, 642
ApeKI GCWGC 2 cut(s) 230, 320
AsuHPI GGTGA 4 cut(s) 88, 333, 558, 599
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 217, 307
BccI CCATC 4 cut(s) 166, 469, 577, 578
BciT130I CCWGG 1 cut(s) 539
BfaI CTAG 2 cut(s) 264, 329
BfrI CTTAAG 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 352
BglII AGATCT 1 cut(s) 406
BisI GCNGC 2 cut(s) 231, 321
BlpI GCTNAGC 1 cut(s) 628
BlsI GCNGC 2 cut(s) 232, 322
Bme1390I CCNGG 1 cut(s) 539
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 2 cut(s) 178, 356
BpiI GAAGAC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 180
Bpu1102I GCTNAGC 1 cut(s) 628
BpuEI CTTGAG 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 135, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 544
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 2 cut(s) 135, 594
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 2 cut(s) 109, 544
BseMII CTCAG 2 cut(s) 171, 204
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 217, 307
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 2 cut(s) 109, 544
BsmFI GGGAC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 406
Bsp1720I GCTNAGC 1 cut(s) 628
BspCNI CTCAG 2 cut(s) 172, 205
BspLI GGNNCC 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 352
BspTI CTTAAG 1 cut(s) 521
BssECI CCNNGG 2 cut(s) 135, 594
BssMI GATC 1 cut(s) 406
BssNAI GTATAC 1 cut(s) 129
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 135, 594
Bst1107I GTATAC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 521
BstAPI GCANNNNNTGC 1 cut(s) 230
BstC8I GCNNGC 3 cut(s) 7, 222, 640
BstDEI CTNAG 4 cut(s) 180, 213, 292, 628
BstF5I GGATG 1 cut(s) 193
BstKTI GATC 1 cut(s) 409
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 4 cut(s) 230, 317, 329, 624
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 537
BstV1I GCAGC 2 cut(s) 217, 307
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 129
BtsCI GGATG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 148, 210
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 3 cut(s) 7, 222, 640
CviAII CATG 1 cut(s) 109
CviJI RGCY 9 cut(s) 134, 179, 320, 353, 376, 526, 618, 627, 642
CviKI_1 RGCY 9 cut(s) 134, 179, 320, 353, 376, 526, 618, 627, 642
DdeI CTNAG 4 cut(s) 180, 213, 292, 628
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
DraIII CACNNNGTG 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 135, 594
Eco24I GRGCYC 1 cut(s) 378
Eco32I GATATC 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 2 cut(s) 135, 594
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 135, 594
FaeI CATG 1 cut(s) 112
FaiI YATR 6 cut(s) 110, 129, 157, 252, 634, 636
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 1 cut(s) 560
FatI CATG 1 cut(s) 108
FauNDI CATATG 1 cut(s) 634
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 2 cut(s) 231, 321
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 2 cut(s) 231, 321
FspBI CTAG 2 cut(s) 264, 329
GluI GCNGC 2 cut(s) 231, 321
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 616
HinfI GANTC 3 cut(s) 335, 530, 590
HphI GGTGA 4 cut(s) 88, 333, 558, 599
Hpy166II GTNNAC 3 cut(s) 82, 129, 298
Hpy188I TCNGA 2 cut(s) 535, 646
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 3 cut(s) 82, 129, 298
Hpy99I CGWCG 1 cut(s) 210
HpyAV CCTTC 3 cut(s) 64, 109, 212
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 9 cut(s) 116, 191, 233, 311, 323, 347, 455, 556, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 317, 329, 624
HpyF3I CTNAG 4 cut(s) 180, 213, 292, 628
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 1 cut(s) 112
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 2 cut(s) 166, 358
LpnPI CCDG 5 cut(s) 357, 524, 529, 540, 551
Lsp1109I GCAGC 2 cut(s) 217, 307
LweI GCATC 2 cut(s) 178, 356
MaeI CTAG 2 cut(s) 264, 329
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 94
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 6 cut(s) 101, 267, 276, 330, 501, 564
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 1 cut(s) 255
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 5 cut(s) 175, 178, 209, 292, 494
MseI TTAA 5 cut(s) 237, 302, 432, 522, 658
MspCI CTTAAG 1 cut(s) 521
MspR9I CCNGG 1 cut(s) 539
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 4 cut(s) 230, 317, 329, 624
NdeI CATATG 1 cut(s) 634
NdeII GATC 1 cut(s) 406
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 94
PaqCI CACCTGC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 202
PfeI GAWTC 2 cut(s) 335, 530
PflMI CCANNNNNTGG 1 cut(s) 109
PkrI GCNGC 2 cut(s) 232, 322
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 354
PsuI RGATCY 1 cut(s) 406
SaqAI TTAA 5 cut(s) 237, 302, 432, 522, 658
SatI GCNGC 2 cut(s) 231, 321
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 2 cut(s) 178, 356
SmlI CTYRAG 2 cut(s) 161, 521
SmoI CTYRAG 2 cut(s) 161, 521
Sse9I AATT 1 cut(s) 255
SspMI CTAG 2 cut(s) 264, 329
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 2 cut(s) 135, 594
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 1 cut(s) 96
TaqI TCGA 2 cut(s) 19, 512
TasI AATT 1 cut(s) 255
TfiI GAWTC 2 cut(s) 335, 530
Tru1I TTAA 5 cut(s) 237, 302, 432, 522, 658
Tru9I TTAA 5 cut(s) 237, 302, 432, 522, 658
TscAI CASTG 2 cut(s) 148, 217
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 230, 320
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 102, 267
TspRI CASTG 2 cut(s) 148, 217
Van91I CCANNNNNTGG 1 cut(s) 109
Vha464I CTTAAG 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 128
XspI CTAG 2 cut(s) 264, 329
ZraI GACGTC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.