Rorug07G0173600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002100
Physical Location & Seq
Forward (+)
14225098 .. 14227125
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0173600.1

Sequence Viewer

Length: 492 bp
ATGGTTCTCAAGACTGAGCTTTGCCGCTTCAGTGGCGCCAAGATCTACCCTGGCAGGGGCATCAGATTCATCCGCTCTGATTCTCAGGTTTTCCTGTTCGTGAATTCCAAGTGCAAGAGGTACTTCCACAACCGCCTCAAGCCGTCCAAGATCACATGGACTGCCATGTTCAGAAAGCAGCACAAGAAGGACTCTGCTCAAGAGTCTGTGAAGAAGAAGCGCCGTGCCACCAAGAAGCCTTACTCAAGGTCCATTGTTGGTGCCACTTTGGAGGTCATCCAGAAGAGGAGGACCGAGAAGCCTGAGGTCCGTGACGCTGCTCGCGAGGCCGCTCTCCGTGAGATTAAGGAGAGAATCAAGAAGACCAAGGATGAGAAGAAGGCAAAGAAAGCAGAAATCACAGCCAAGTCAAGCAAGCCTACCAAGGGTAATCTGTCCAAGGCTGCAGCCCCCAAGGGCCCCAAGCTTGGTGGTGGAGGTGGAAAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.5

Weight (kDa)

10.86

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L24e PF01246 3 - 67 6.3e-28 Ribosomal protein L24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 35, 260
AccBSI CCGCTC 2 cut(s) 75, 332
AccII CGCG 1 cut(s) 324
AciI CCGC 4 cut(s) 25, 73, 133, 330
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 13
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 4 cut(s) 55, 56, 425, 467
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 2 cut(s) 19, 466
AluI AGCT 2 cut(s) 19, 466
AoxI GGCC 2 cut(s) 327, 457
ApaI GGGCCC 1 cut(s) 461
ApeKI GCWGC 4 cut(s) 178, 317, 443, 446
ApoI RAATTY 1 cut(s) 103
AspLEI GCGC 2 cut(s) 38, 222
AspS9I GGNCC 5 cut(s) 249, 291, 307, 457, 458
AvaII GGWCC 3 cut(s) 249, 291, 307
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 461
BanI GGYRCC 2 cut(s) 35, 260
BanII GRGCYC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 4 cut(s) 190, 304, 430, 458
BceAI ACGGC 2 cut(s) 127, 207
BciT130I CCWGG 1 cut(s) 51
BfmI CTRYAG 1 cut(s) 444
BfoI RGCGCY 2 cut(s) 39, 223
BglII AGATCT 1 cut(s) 42
BisI GCNGC 6 cut(s) 25, 179, 318, 330, 444, 447
BlsI GCNGC 6 cut(s) 26, 180, 319, 331, 445, 448
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 3 cut(s) 249, 291, 307
BmgT120I GGNCC 5 cut(s) 249, 291, 307, 457, 458
BmiI GGNNCC 4 cut(s) 37, 262, 459, 460
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 368
BpuEI CTTGAG 3 cut(s) 122, 183, 229
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 5 cut(s) 49, 366, 423, 438, 453
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 56, 425, 467
Bse21I CCTNAGG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 5 cut(s) 49, 366, 423, 438, 453
BseGI GGATG 3 cut(s) 69, 276, 376
BseLI CCNNNNNNNGG 4 cut(s) 55, 56, 425, 467
BseMII CTCAG 3 cut(s) 6, 98, 294
BseRI GAGGAG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 4 cut(s) 190, 304, 430, 458
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 2 cut(s) 329, 459
BshNI GGYRCC 2 cut(s) 35, 260
BslI CCNNNNNNNGG 4 cut(s) 55, 56, 425, 467
BsnI GGCC 2 cut(s) 329, 459
Bsp120I GGGCCC 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 2 cut(s) 42, 150
Bsp68I TCGCGA 1 cut(s) 324
BspACI CCGC 4 cut(s) 25, 73, 133, 330
BspANI GGCC 2 cut(s) 329, 459
BspCNI CTCAG 3 cut(s) 7, 97, 295
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 4 cut(s) 37, 262, 459, 460
BspMAI CTGCAG 1 cut(s) 448
BspT107I GGYRCC 2 cut(s) 35, 260
BsrBI CCGCTC 2 cut(s) 75, 332
BssECI CCNNGG 5 cut(s) 49, 366, 423, 438, 453
BssMI GATC 2 cut(s) 42, 150
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 4 cut(s) 366, 423, 438, 453
Bst2UI CCWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 278
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 322, 416
BstDEI CTNAG 3 cut(s) 15, 84, 303
BstF5I GGATG 3 cut(s) 69, 276, 376
BstFNI CGCG 1 cut(s) 324
BstH2I RGCGCY 2 cut(s) 39, 223
BstHHI GCGC 2 cut(s) 38, 222
BstKTI GATC 2 cut(s) 45, 153
BstMBI GATC 2 cut(s) 42, 150
BstMWI GCNNNNNNNGC 3 cut(s) 33, 326, 389
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 4 cut(s) 190, 304, 430, 458
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 2 cut(s) 329, 459
BtsCI GGATG 3 cut(s) 69, 276, 376
BtsIMutI CAGTG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 322, 416
CfoI GCGC 2 cut(s) 38, 222
Cfr13I GGNCC 5 cut(s) 249, 291, 307, 457, 458
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 121
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 2 cut(s) 156, 166
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 3 cut(s) 15, 84, 303
DinI GGCGCC 1 cut(s) 37
DpnI GATC 2 cut(s) 44, 152
DpnII GATC 2 cut(s) 42, 150
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco130I CCWWGG 4 cut(s) 366, 423, 438, 453
Eco24I GRGCYC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 249, 291, 307
Eco57I CTGAAG 1 cut(s) 13
Eco81I CCTNAGG 1 cut(s) 303
EcoO109I RGGNCCY 2 cut(s) 457, 458
EcoRI GAATTC 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 4 cut(s) 366, 423, 438, 453
EcoT38I GRGCYC 1 cut(s) 461
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 4 cut(s) 366, 423, 438, 453
FaeI CATG 2 cut(s) 159, 169
FaiI YATR 2 cut(s) 157, 167
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 2 cut(s) 155, 165
Fnu4HI GCNGC 6 cut(s) 25, 179, 318, 330, 444, 447
FokI GGATG 3 cut(s) 56, 263, 383
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 6 cut(s) 25, 179, 318, 330, 444, 447
GlaI GCGC 2 cut(s) 37, 221
GluI GCNGC 6 cut(s) 25, 179, 318, 330, 444, 447
HaeII RGCGCY 2 cut(s) 39, 223
HaeIII GGCC 2 cut(s) 329, 459
HgaI GACGC 1 cut(s) 323
HhaI GCGC 2 cut(s) 38, 222
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 2 cut(s) 159, 169
Hin6I GCGC 2 cut(s) 36, 220
HinP1I GCGC 2 cut(s) 36, 220
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 5 cut(s) 66, 80, 191, 203, 354
Hpy188I TCNGA 3 cut(s) 65, 79, 173
Hpy188III TCNNGA 6 cut(s) 10, 100, 200, 280, 323, 358
HpyAV CCTTC 2 cut(s) 181, 373
HpyCH4V TGCA 2 cut(s) 114, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 326, 389
HpyF3I CTNAG 3 cut(s) 15, 84, 303
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 2 cut(s) 159, 169
HspAI GCGC 2 cut(s) 36, 220
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 2 cut(s) 42, 150
LpnPI CCDG 7 cut(s) 36, 40, 63, 71, 107, 293, 315
Lsp1109I GCAGC 4 cut(s) 190, 304, 430, 458
LweI GCATC 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 44, 152
MbiI CCGCTC 2 cut(s) 75, 332
MboI GATC 2 cut(s) 42, 150
MboII GAAGA 5 cut(s) 223, 226, 295, 373, 388
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 1 cut(s) 103
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 2 cut(s) 185, 212
MnlI CCTC 8 cut(s) 111, 146, 265, 279, 282, 298, 319, 470
MseI TTAA 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 3 cut(s) 33, 326, 389
NarI GGCGCC 1 cut(s) 36
NdeII GATC 2 cut(s) 42, 150
NlaIII CATG 2 cut(s) 159, 169
NlaIV GGNNCC 4 cut(s) 37, 262, 459, 460
NmuCI GTSAC 1 cut(s) 311
NruI TCGCGA 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PfeI GAWTC 3 cut(s) 66, 80, 354
PkrI GCNGC 6 cut(s) 26, 180, 319, 331, 445, 448
PleI GAGTC 2 cut(s) 185, 211
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 2 cut(s) 185, 211
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 4 cut(s) 37, 262, 459, 460
PspOMI GGGCCC 1 cut(s) 457
PspPI GGNCC 5 cut(s) 249, 291, 307, 457, 458
PstI CTGCAG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 42
RruI TCGCGA 1 cut(s) 324
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 6 cut(s) 25, 179, 318, 330, 444, 447
Sau3AI GATC 2 cut(s) 42, 150
Sau96I GGNCC 5 cut(s) 249, 291, 307, 457, 458
SchI GAGTC 2 cut(s) 185, 212
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 1 cut(s) 461
SetI ASST 8 cut(s) 21, 90, 122, 251, 276, 309, 468, 481
SfaNI GCATC 1 cut(s) 69
SfcI CTRYAG 1 cut(s) 444
SfoI GGCGCC 1 cut(s) 37
SinI GGWCC 3 cut(s) 249, 291, 307
SmlI CTYRAG 4 cut(s) 8, 137, 198, 244
SmoI CTYRAG 4 cut(s) 8, 137, 198, 244
Sse9I AATT 1 cut(s) 103
SsiI CCGC 4 cut(s) 25, 73, 133, 330
SspDI GGCGCC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 4 cut(s) 366, 423, 438, 453
TaqII GACCGA 1 cut(s) 308
TasI AATT 1 cut(s) 103
TauI GCSGC 2 cut(s) 27, 332
TfiI GAWTC 3 cut(s) 66, 80, 354
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 4 cut(s) 178, 317, 443, 446
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 1 cut(s) 58
TspGWI ACGGA 2 cut(s) 299, 326
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 3 cut(s) 249, 291, 307
XapI RAATTY 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.