Rroxscaffold_3G00245180

Src homology 3 domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004165
Physical Location & Seq
Forward (+)
38012746 .. 38015508
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245180.1

Sequence Viewer

Length: 540 bp
ATGATTCTGGAGAAGCTACCACAAGAATTTTCATTAGAATCAGAGAAGGTGCATCCAAATGCTAGTTCAAAGAGATCCGATGATCATGAACAACTTACTCGAGATGGCAATTTTTTCATTGCAAGAGCTATACACCCATTTGATGCTCAAGCAGATGGAGAGTTGAATCTATCAATTGATGATTATGTTGTGGTTCGTGAGGTGGGCCCTAATGGATGGTCTGGAGGAGAATGCAAGGGCAAGGCCGGGTGGTTTCCAGCAGGAAAAAGCACCAGTGAGCAAATTATTCTTGGATTTTCAGCAAAATTAGTGAAAGAAAATAAGGAAATTGGGTCTGCACCGAGGCTCGAGGATGAAATGAGCAGAGCCAAAGCAGCTAAGAGGGTGGAAATGGATAATGAGACCATGGCTGAGACCCGAGATGGATTTGAGCTGAATGCGGGTTTGGGGGGTTTCGGTGAGAGAGGCAATTGCATCTTAAAGGTGAGTTATATCTACGGTTTGAAGGGTAGAAAGCAGAAGGAGAAAAGTAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
SH3_9

Protein Analysis

179

Amino Acids

19.8

Weight (kDa)

5.77

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 42 - 87 9e-08 SH3 domain
SH3_9 PF14604 43 - 87 6.7e-08 Variant SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 440
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 1 cut(s) 26
AgsI TTSAA 3 cut(s) 69, 166, 505
AjuI GAANNNNNNNTTGG 4 cut(s) 49, 81, 428, 460
AluBI AGCT 4 cut(s) 16, 128, 377, 433
AluI AGCT 4 cut(s) 16, 128, 377, 433
Alw26I GTCTC 2 cut(s) 395, 407
AlwI GGATC 1 cut(s) 69
Ama87I CYCGRG 3 cut(s) 99, 347, 417
AoxI GGCC 2 cut(s) 205, 243
ApaI GGGCCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 205, 206
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 2 cut(s) 470, 496
AvaI CYCGRG 3 cut(s) 99, 347, 417
BaeGI GKGCMC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 386
BccI CCATC 4 cut(s) 98, 149, 210, 416
BclI TGATCA 1 cut(s) 82
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 395, 407
BfaI CTAG 1 cut(s) 63
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 247
BmeT110I CYCGRG 3 cut(s) 99, 347, 417
BmgT120I GGNCC 2 cut(s) 205, 206
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 3 cut(s) 61, 133, 483
BpmI CTGGAG 2 cut(s) 29, 243
BpuEI CTTGAG 1 cut(s) 132
BpuMI CCSGG 1 cut(s) 247
BsaI GGTCTC 2 cut(s) 395, 407
BsaJI CCNNGG 2 cut(s) 341, 405
Bse1I ACTGG 1 cut(s) 273
Bse3DI GCAATG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 341, 405
BseGI GGATG 3 cut(s) 52, 221, 358
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 240
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 1 cut(s) 386
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 2 cut(s) 207, 245
BsiHKCI CYCGRG 3 cut(s) 99, 347, 417
BsiSI CCGG 1 cut(s) 246
BsmAI GTCTC 2 cut(s) 395, 407
BsmI GAATGC 2 cut(s) 236, 442
BsnI GGCC 2 cut(s) 207, 245
Bso31I GGTCTC 2 cut(s) 395, 407
BsoBI CYCGRG 3 cut(s) 99, 347, 417
Bsp120I GGGCCC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 2 cut(s) 74, 82
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 1 cut(s) 440
BspANI GGCC 2 cut(s) 207, 245
BspCNI CTCAG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 1 cut(s) 69
BspTNI GGTCTC 2 cut(s) 395, 407
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 1 cut(s) 273
BssECI CCNNGG 2 cut(s) 341, 405
BssMI GATC 2 cut(s) 74, 82
BssT1I CCWWGG 1 cut(s) 405
Bst4CI ACNGT 1 cut(s) 500
BstDEI CTNAG 2 cut(s) 378, 411
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 52, 221, 358
BstKTI GATC 2 cut(s) 77, 85
BstMAI GTCTC 2 cut(s) 395, 407
BstMBI GATC 2 cut(s) 74, 82
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 209
BstV1I GCAGC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BsuRI GGCC 2 cut(s) 207, 245
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 3 cut(s) 52, 221, 358
BtsIMutI CAGTG 1 cut(s) 280
CciI TCATGA 1 cut(s) 85
Cfr13I GGNCC 2 cut(s) 205, 206
CviAII CATG 2 cut(s) 86, 406
CviJI RGCY 9 cut(s) 16, 128, 207, 245, 346, 368, 377, 410, 433
CviKI_1 RGCY 9 cut(s) 16, 128, 207, 245, 346, 368, 377, 410, 433
DdeI CTNAG 2 cut(s) 378, 411
DpnI GATC 2 cut(s) 76, 84
DpnII GATC 2 cut(s) 74, 82
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 2 cut(s) 395, 407
Eco88I CYCGRG 3 cut(s) 99, 347, 417
EcoO109I RGGNCCY 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 2 cut(s) 89, 409
FaiI YATR 5 cut(s) 87, 131, 186, 407, 492
FatI CATG 2 cut(s) 85, 405
FauI CCCGC 1 cut(s) 433
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 3 cut(s) 39, 228, 365
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 1 cut(s) 63
GluI GCNGC 1 cut(s) 375
GsuI CTGGAG 2 cut(s) 29, 243
HaeIII GGCC 2 cut(s) 207, 245
HapII CCGG 1 cut(s) 246
Hin1II CATG 2 cut(s) 89, 409
HinfI GANTC 3 cut(s) 4, 38, 166
HpaII CCGG 1 cut(s) 246
HphI GGTGA 2 cut(s) 470, 496
Hpy188I TCNGA 2 cut(s) 43, 79
Hpy188III TCNNGA 5 cut(s) 8, 86, 101, 197, 222
HpyAV CCTTC 3 cut(s) 40, 499, 514
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4V TGCA 5 cut(s) 52, 122, 234, 338, 474
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 2 cut(s) 378, 411
Hsp92II CATG 2 cut(s) 89, 409
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 2 cut(s) 74, 82
LpnPI CCDG 5 cut(s) 207, 246, 259, 270, 286
Lsp1109I GCAGC 1 cut(s) 386
LweI GCATC 3 cut(s) 61, 133, 483
MaeI CTAG 1 cut(s) 63
MalI GATC 2 cut(s) 76, 84
MboI GATC 2 cut(s) 74, 82
MfeI CAATTG 2 cut(s) 174, 469
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 7 cut(s) 26, 109, 174, 282, 305, 327, 469
MnlI CCTC 6 cut(s) 193, 218, 336, 343, 375, 458
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 57
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 2 cut(s) 174, 469
Mva1269I GAATGC 2 cut(s) 236, 442
MwoI GCNNNNNNNGC 1 cut(s) 374
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 405
NdeII GATC 2 cut(s) 74, 82
NlaIII CATG 2 cut(s) 89, 409
NlaIV GGNNCC 1 cut(s) 207
PaeR7I CTCGAG 2 cut(s) 99, 347
PagI TCATGA 1 cut(s) 85
PctI GAATGC 2 cut(s) 236, 442
PfeI GAWTC 3 cut(s) 4, 38, 166
PkrI GCNGC 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 207
PspOMI GGGCCC 1 cut(s) 205
PspPI GGNCC 2 cut(s) 205, 206
PspXI VCTCGAGB 1 cut(s) 347
PsuI RGATCY 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 57
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 2 cut(s) 74, 82
Sau96I GGNCC 2 cut(s) 205, 206
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 1 cut(s) 209
SetI ASST 7 cut(s) 18, 51, 130, 204, 379, 435, 486
SfaNI GCATC 3 cut(s) 61, 133, 483
Sfr274I CTCGAG 2 cut(s) 99, 347
SlaI CTCGAG 2 cut(s) 99, 347
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 3 cut(s) 99, 147, 347
SmoI CTYRAG 3 cut(s) 99, 147, 347
Sse9I AATT 7 cut(s) 26, 109, 174, 282, 305, 327, 469
SsiI CCGC 1 cut(s) 440
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 500
TaqI TCGA 2 cut(s) 100, 348
TasI AATT 7 cut(s) 26, 109, 174, 282, 305, 327, 469
TfiI GAWTC 3 cut(s) 4, 38, 166
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 1 cut(s) 280
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 4 cut(s) 21, 102, 106, 369
TspRI CASTG 1 cut(s) 280
XapI RAATTY 1 cut(s) 26
XhoI CTCGAG 2 cut(s) 99, 347
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.