Rroxscaffold_3G00255750

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013743
Physical Location & Seq
Forward (+)
50148418 .. 50151863
3446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255750.1

Sequence Viewer

Length: 864 bp
ATGGCATTCACATCCTTGTTTTGTATCCAACTCACAGGCGTCATGCAACTGGACAGCAACCGGAGATCTTTTGTGTATAATGATGGGGTTGCTGGGAAGAGTTTAGGCCGGAAAATCATGACTGATGGGAATCTGAAGGGTAAGGCAATGCTTGAAATGCAGGCTACTGAAGGAGAACCATCACTGCCCTGGGATGTATCTAGTCATGTTTTTGATCTTGGATTGAATACCTTGCTGAAATTCATTTTAATCAACCTGTACAGATGTGGGTACATTAATCAGTTGGAGGAGGACAGAGATGTTGTTGCTCAGCACAAGCAATTGCAATGCTTGAATCCTTACCTCAGCTTCCATTTTCTGTTGTTAATGCTCTGCATCATTGACTCCAAGGTATTATCAGATTCATCGCCTTCTCAAGAGTTAGAATTGAGGCAGCCTTTGGATTGGCTAATACATCATCTGAGGTACCACAATTCGGTTATAGGAGCTTTGCAGAGCGTCAATGGCATGTTTAGGGCATCCGCGGCATTGCTTCATAACCGGGCTGATGTGGTTTTTGATCCCAGATTGGTCAAGGAAGCGGATAAGGAGAAGCAGAAGAGGGAGTCCGAAAAAATAGGAGATGCCATGAAATCTTTGGAGAACAGAACCTTGGATTCGAAATGGGAGATTGATATCCTTGCCGCTTTGGATGAGATGAAGTCCATGAAGGGAGAGACAAGTCTGAGGAAGGCTGCTGAAGAGGAAGCCAGTAATCTTAAGAGTCAACTGACTCAATGGAAAAGGTCAAAGGCAGCTGGAAATTCAGAGAGCTTAAAGCTTCGTAAGATGCTGAAAGATGGGGCACATCAGAAGATGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

287

Amino Acids

32.56

Weight (kDa)

8.54

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 192 - 264 5.3e-08 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 465
AccB1I GGYRCC 1 cut(s) 465
AccII CGCG 1 cut(s) 524
AciI CCGC 4 cut(s) 522, 524, 581, 684
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 2 cut(s) 239, 802
AcuI CTGAAG 3 cut(s) 155, 189, 759
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 260, 272, 467
AfiI CCNNNNNNNGG 1 cut(s) 475
AflII CTTAAG 1 cut(s) 758
AgsI TTSAA 3 cut(s) 155, 226, 334
AjnI CCWGG 1 cut(s) 188
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AluBI AGCT 5 cut(s) 348, 488, 797, 813, 820
AluI AGCT 5 cut(s) 348, 488, 797, 813, 820
Alw26I GTCTC 1 cut(s) 710
AlwI GGATC 1 cut(s) 554
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 3 cut(s) 433, 734, 794
ApoI RAATTY 2 cut(s) 239, 802
AseI ATTAAT 1 cut(s) 276
Asp718I GGTACC 1 cut(s) 465
AsuC2I CCSGG 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 659
BaeGI GKGCMC 1 cut(s) 847
BanI GGYRCC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 344
BbvI GCAGC 3 cut(s) 445, 721, 806
BccI CCATC 4 cut(s) 77, 119, 187, 833
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 1 cut(s) 35
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 710
BfaI CTAG 1 cut(s) 201
BfrI CTTAAG 1 cut(s) 758
BfuI GTATCC 1 cut(s) 35
BglII AGATCT 1 cut(s) 65
BisI GCNGC 5 cut(s) 434, 525, 684, 735, 795
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 5 cut(s) 435, 526, 685, 736, 796
Bme1390I CCNGG 2 cut(s) 190, 542
BmiI GGNNCC 1 cut(s) 467
BmrFI CCNGG 2 cut(s) 190, 542
BmsI GCATC 4 cut(s) 384, 527, 613, 819
Bpu10I CCTNAGC 1 cut(s) 344
Bpu1102I GCTNAGC 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 399
BpuMI CCSGG 1 cut(s) 542
BsaBI GATNNNNATC 2 cut(s) 129, 674
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 5 cut(s) 188, 189, 387, 522, 651
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 2 cut(s) 54, 750
Bse3DI GCAATG 3 cut(s) 153, 332, 527
Bse8I GATNNNNATC 2 cut(s) 129, 674
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 5 cut(s) 188, 189, 387, 522, 651
BseGI GGATG 4 cut(s) 11, 199, 518, 697
BseJI GATNNNNATC 2 cut(s) 129, 674
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 3 cut(s) 153, 332, 527
BseMII CTCAG 4 cut(s) 323, 358, 452, 716
BseNI ACTGG 2 cut(s) 54, 750
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 3 cut(s) 445, 721, 806
BseYI CCCAGC 1 cut(s) 92
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 1 cut(s) 108
BshNI GGYRCC 1 cut(s) 465
BsiSI CCGG 3 cut(s) 61, 109, 541
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 710
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 1 cut(s) 847
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 3 cut(s) 65, 214, 559
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 4 cut(s) 522, 524, 581, 684
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 4 cut(s) 322, 357, 453, 717
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 467
BspPI GGATC 1 cut(s) 554
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 465
BspTI CTTAAG 1 cut(s) 758
BsrDI GCAATG 3 cut(s) 153, 332, 527
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 54, 750
BssECI CCNNGG 5 cut(s) 188, 189, 387, 522, 651
BssMI GATC 3 cut(s) 65, 214, 559
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 387, 651
Bst2UI CCWGG 1 cut(s) 190
Bst6I CTCTTC 3 cut(s) 92, 593, 735
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 758
BstAUI TGTACA 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 4 cut(s) 309, 344, 461, 725
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 11, 199, 518, 697
BstFNI CGCG 1 cut(s) 524
BstKTI GATC 3 cut(s) 68, 217, 562
BstMAI GTCTC 1 cut(s) 710
BstMBI GATC 3 cut(s) 65, 214, 559
BstMWI GCNNNNNNNGC 3 cut(s) 157, 504, 524
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 188, 540
BstSLI GKGCMC 1 cut(s) 847
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 445, 721, 806
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuI GTATCC 1 cut(s) 35
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 522
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 4 cut(s) 11, 199, 518, 697
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 162
CciI TCATGA 1 cut(s) 117
Cfr42I CCGCGG 1 cut(s) 525
CseI GACGC 2 cut(s) 28, 487
Csp6I GTAC 3 cut(s) 259, 271, 466
CviAII CATG 6 cut(s) 43, 118, 206, 508, 628, 706
CviQI GTAC 3 cut(s) 259, 271, 466
DdeI CTNAG 4 cut(s) 309, 344, 461, 725
DpnI GATC 3 cut(s) 67, 216, 561
DpnII GATC 3 cut(s) 65, 214, 559
Eam1104I CTCTTC 3 cut(s) 92, 593, 735
EarI CTCTTC 3 cut(s) 92, 593, 735
Eco130I CCWWGG 2 cut(s) 387, 651
Eco32I GATATC 1 cut(s) 676
Eco57I CTGAAG 3 cut(s) 155, 189, 759
EcoRII CCWGG 1 cut(s) 188
EcoRV GATATC 1 cut(s) 676
EcoT14I CCWWGG 2 cut(s) 387, 651
ErhI CCWWGG 2 cut(s) 387, 651
FaeI CATG 6 cut(s) 46, 121, 209, 511, 631, 709
FaiI YATR 9 cut(s) 44, 78, 119, 207, 482, 509, 537, 629, 707
FatI CATG 6 cut(s) 42, 117, 205, 507, 627, 705
Fnu4HI GCNGC 5 cut(s) 434, 525, 684, 735, 795
FokI GGATG 3 cut(s) 206, 505, 704
Fsp4HI GCNGC 5 cut(s) 434, 525, 684, 735, 795
FspBI CTAG 1 cut(s) 201
GluI GCNGC 5 cut(s) 434, 525, 684, 735, 795
GsaI CCCAGC 1 cut(s) 96
HaeIII GGCC 1 cut(s) 108
HapII CCGG 3 cut(s) 61, 109, 541
HgaI GACGC 2 cut(s) 28, 487
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 46, 121, 209, 511, 631, 709
HincII GTYRAC 1 cut(s) 767
HindII GTYRAC 1 cut(s) 767
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 8 cut(s) 130, 334, 383, 401, 605, 656, 763, 772
HpaII CCGG 3 cut(s) 61, 109, 541
Hpy166II GTNNAC 1 cut(s) 767
Hpy188I TCNGA 7 cut(s) 135, 400, 462, 610, 726, 808, 852
Hpy188III TCNNGA 2 cut(s) 118, 416
Hpy8I GTNNAC 1 cut(s) 767
HpyAV CCTTC 5 cut(s) 130, 164, 420, 703, 724
HpyCH4V TGCA 5 cut(s) 46, 160, 325, 375, 493
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 504, 524
HpyF3I CTNAG 4 cut(s) 309, 344, 461, 725
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 46, 121, 209, 511, 631, 709
KpnI GGTACC 1 cut(s) 469
KspI CCGCGG 1 cut(s) 525
Kzo9I GATC 3 cut(s) 65, 214, 559
LmnI GCTCC 1 cut(s) 485
Lsp1109I GCAGC 3 cut(s) 445, 721, 806
LweI GCATC 4 cut(s) 384, 527, 613, 819
MaeI CTAG 1 cut(s) 201
MalI GATC 3 cut(s) 67, 216, 561
MboI GATC 3 cut(s) 65, 214, 559
MboII GAAGA 3 cut(s) 109, 610, 752
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 1 cut(s) 847
MluCI AATT 5 cut(s) 239, 320, 425, 472, 802
MlyI GAGTC 4 cut(s) 377, 614, 766, 772
MmeI TCCRAC 2 cut(s) 52, 264
MnlI CCTC 8 cut(s) 280, 283, 353, 423, 456, 594, 720, 736
MseI TTAA 5 cut(s) 248, 276, 365, 759, 815
MspA1I CMGCKG 2 cut(s) 524, 797
MspCI CTTAAG 1 cut(s) 758
MspI CCGG 3 cut(s) 61, 109, 541
MspR9I CCNGG 2 cut(s) 190, 542
MunI CAATTG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 157, 504, 524
NciI CCSGG 1 cut(s) 542
NdeII GATC 3 cut(s) 65, 214, 559
NlaIII CATG 6 cut(s) 46, 121, 209, 511, 631, 709
NlaIV GGNNCC 1 cut(s) 467
NspI RCATGY 1 cut(s) 511
NspV TTCGAA 1 cut(s) 659
PagI TCATGA 1 cut(s) 117
PasI CCCWGGG 1 cut(s) 189
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 4 cut(s) 130, 334, 401, 656
PkrI GCNGC 5 cut(s) 435, 526, 685, 736, 796
PleI GAGTC 4 cut(s) 377, 613, 766, 771
PpsI GAGTC 4 cut(s) 377, 613, 766, 771
PshBI ATTAAT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 467
PsuI RGATCY 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 3 cut(s) 260, 272, 467
RsaNI GTAC 3 cut(s) 259, 271, 466
SacII CCGCGG 1 cut(s) 525
SaqAI TTAA 5 cut(s) 248, 276, 365, 759, 815
SatI GCNGC 5 cut(s) 434, 525, 684, 735, 795
Sau3AI GATC 3 cut(s) 65, 214, 559
SchI GAGTC 4 cut(s) 377, 614, 766, 772
ScrFI CCNGG 2 cut(s) 190, 542
SduI GDGCHC 1 cut(s) 847
SfaNI GCATC 4 cut(s) 384, 527, 613, 819
Sfr303I CCGCGG 1 cut(s) 525
SfuI TTCGAA 1 cut(s) 659
SgrBI CCGCGG 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 414, 758
SmoI CTYRAG 2 cut(s) 414, 758
Sse9I AATT 5 cut(s) 239, 320, 425, 472, 802
SsiI CCGC 4 cut(s) 522, 524, 581, 684
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 188, 540
StyI CCWWGG 2 cut(s) 387, 651
TaqI TCGA 1 cut(s) 659
TasI AATT 5 cut(s) 239, 320, 425, 472, 802
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 527, 686
TfiI GAWTC 4 cut(s) 130, 334, 401, 656
Tru1I TTAA 5 cut(s) 248, 276, 365, 759, 815
Tru9I TTAA 5 cut(s) 248, 276, 365, 759, 815
TscAI CASTG 1 cut(s) 189
TseI GCWGC 3 cut(s) 433, 734, 794
TspDTI ATGAA 6 cut(s) 232, 393, 524, 644, 713, 722
TspRI CASTG 1 cut(s) 189
Vha464I CTTAAG 1 cut(s) 758
VspI ATTAAT 1 cut(s) 276
XapI RAATTY 2 cut(s) 239, 802
XceI RCATGY 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 2 cut(s) 186, 634
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.