Rroxscaffold_3G00255780

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013743
Physical Location & Seq
Forward (+)
50189191 .. 50199414
10224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255780.1

Sequence Viewer

Length: 471 bp
ATGTATCACGAGGAGAGTAGTTTTATGAAGTTTTGTTCTTTGATTCCAAGGCCTTCTGGAGAGTTCAAATTGAGGCAGCCTTTGGATTGGCCGATACATCATCTGAGGGTACACAATTCGGTTGAAGTAGCTTTGCAGAGAATCAATGACATGTTTAGGCCATCCGCGGCGTTGCTTCAGAACCTGGCTGATGTGGTGTTTGGTCCCAGATTGGTCAAGGAAGCGGATAAGGAGAGGCAGAAGAGGGAGTCCAAAAAAATGGGAGATGCCATGAAATCTTTGGAGAACAGAACCTTGGATTCGAAATGGGAGATTGATATCCTTGCCACTTTGGAAGAGATGAAGTCTATGAAGATTAGCAGGTTGCATGTTGAGGAAATCGGTGACTTAAGAAAGTTGCTCCTGGGAGAGCCAAGTCATGGGAAGGCTTCTGAAGAGGAAGCCAGTAATCTTAAGAGTCAACTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

156

Amino Acids

17.99

Weight (kDa)

7.03

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 73 - 148 2.3e-10 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 419
AccII CGCG 1 cut(s) 167
AciI CCGC 3 cut(s) 165, 167, 224
AcoI YGGCCR 1 cut(s) 89
AcuI CTGAAG 2 cut(s) 161, 453
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 419
AflII CTTAAG 2 cut(s) 388, 452
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 2 cut(s) 67, 125
AjnI CCWGG 2 cut(s) 183, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 1 cut(s) 131
AluI AGCT 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 184
AoxI GGCC 3 cut(s) 50, 89, 158
ApeKI GCWGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 203
BauI CACGAG 1 cut(s) 8
BbvI GCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 169
BciT130I CCWGG 2 cut(s) 185, 404
BfrI CTTAAG 2 cut(s) 388, 452
BfuAI ACCTGC 1 cut(s) 351
BisI GCNGC 2 cut(s) 77, 168
BlsI GCNGC 2 cut(s) 78, 169
Bme1390I CCNGG 2 cut(s) 185, 404
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 2 cut(s) 185, 404
BmsI GCATC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 47, 165, 294, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 317
BseBI CCWGG 2 cut(s) 185, 404
BseDI CCNNGG 4 cut(s) 47, 165, 294, 403
BseGI GGATG 1 cut(s) 161
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 1 cut(s) 444
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 3 cut(s) 52, 91, 160
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 3 cut(s) 52, 91, 160
Bsp119I TTCGAA 1 cut(s) 302
BspACI CCGC 3 cut(s) 165, 167, 224
BspANI GGCC 3 cut(s) 52, 91, 160
BspCNI CTCAG 1 cut(s) 96
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 351
BspT104I TTCGAA 1 cut(s) 302
BspTI CTTAAG 2 cut(s) 388, 452
BsrI ACTGG 1 cut(s) 444
BssECI CCNNGG 4 cut(s) 47, 165, 294, 403
BssSI CACGAG 1 cut(s) 8
BssT1I CCWWGG 2 cut(s) 47, 294
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 2 cut(s) 185, 404
Bst6I CTCTTC 3 cut(s) 236, 330, 429
BstAFI CTTAAG 2 cut(s) 388, 452
BstBI TTCGAA 1 cut(s) 302
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 161
BstFNI CGCG 1 cut(s) 167
BstNI CCWGG 2 cut(s) 185, 404
BstNSI RCATGY 2 cut(s) 154, 371
BstSCI CCNGG 2 cut(s) 183, 402
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 3 cut(s) 52, 91, 160
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 1 cut(s) 161
BveI ACCTGC 1 cut(s) 351
CaiI CAGNNNCTG 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 203
Cfr42I CCGCGG 1 cut(s) 168
Csp6I GTAC 1 cut(s) 110
CviAII CATG 4 cut(s) 151, 271, 368, 419
CviJI RGCY 9 cut(s) 52, 79, 91, 131, 160, 188, 412, 428, 443
CviKI_1 RGCY 9 cut(s) 52, 79, 91, 131, 160, 188, 412, 428, 443
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 3 cut(s) 236, 330, 429
EarI CTCTTC 3 cut(s) 236, 330, 429
Eco130I CCWWGG 2 cut(s) 47, 294
Eco147I AGGCCT 1 cut(s) 52
Eco32I GATATC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 2 cut(s) 161, 453
EcoRII CCWGG 2 cut(s) 183, 402
EcoRV GATATC 1 cut(s) 319
EcoT14I CCWWGG 2 cut(s) 47, 294
ErhI CCWWGG 2 cut(s) 47, 294
FaeI CATG 4 cut(s) 154, 274, 371, 422
FaiI YATR 6 cut(s) 26, 152, 272, 350, 369, 420
FaqI GGGAC 1 cut(s) 189
FatI CATG 4 cut(s) 150, 270, 367, 418
Fnu4HI GCNGC 2 cut(s) 77, 168
FokI GGATG 1 cut(s) 148
Fsp4HI GCNGC 2 cut(s) 77, 168
GluI GCNGC 2 cut(s) 77, 168
GsuI CTGGAG 1 cut(s) 78
HaeIII GGCC 3 cut(s) 52, 91, 160
Hin1II CATG 4 cut(s) 154, 274, 371, 422
HincII GTYRAC 1 cut(s) 461
HindII GTYRAC 1 cut(s) 461
HinfI GANTC 5 cut(s) 43, 141, 248, 299, 457
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 112, 461
Hpy188I TCNGA 3 cut(s) 105, 180, 433
Hpy188III TCNNGA 2 cut(s) 8, 57
Hpy8I GTNNAC 2 cut(s) 112, 461
HpyAV CCTTC 2 cut(s) 63, 418
HpyCH4V TGCA 2 cut(s) 136, 367
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 4 cut(s) 154, 274, 371, 422
KspI CCGCGG 1 cut(s) 168
LmnI GCTCC 1 cut(s) 405
LpnPI CCDG 8 cut(s) 42, 170, 197, 220, 346, 389, 416, 457
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 1 cut(s) 256
MaeIII GTNAC 1 cut(s) 383
MboII GAAGA 4 cut(s) 253, 347, 364, 446
MluCI AATT 2 cut(s) 68, 115
MlyI GAGTC 2 cut(s) 257, 466
MnlI CCTC 7 cut(s) 4, 66, 99, 228, 237, 367, 430
MseI TTAA 3 cut(s) 389, 453, 469
MspA1I CMGCKG 1 cut(s) 167
MspCI CTTAAG 2 cut(s) 388, 452
MspR9I CCNGG 2 cut(s) 185, 404
MvaI CCWGG 2 cut(s) 185, 404
MvnI CGCG 1 cut(s) 167
NlaIII CATG 4 cut(s) 154, 274, 371, 422
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 2 cut(s) 154, 371
NspV TTCGAA 1 cut(s) 302
PceI AGGCCT 1 cut(s) 52
PciI ACATGT 1 cut(s) 150
PfeI GAWTC 3 cut(s) 43, 141, 299
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 2 cut(s) 78, 169
PleI GAGTC 2 cut(s) 256, 465
PpsI GAGTC 2 cut(s) 256, 465
PscI ACATGT 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 183, 402
PspGI CCWGG 2 cut(s) 183, 402
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 184
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SacII CCGCGG 1 cut(s) 168
SaqAI TTAA 3 cut(s) 389, 453, 469
SatI GCNGC 2 cut(s) 77, 168
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 257, 466
ScrFI CCNGG 2 cut(s) 185, 404
SetI ASST 4 cut(s) 133, 186, 296, 365
SfaNI GCATC 1 cut(s) 256
Sfr303I CCGCGG 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 302
SgrBI CCGCGG 1 cut(s) 168
SinI GGWCC 1 cut(s) 203
SmlI CTYRAG 2 cut(s) 388, 452
SmoI CTYRAG 2 cut(s) 388, 452
Sse9I AATT 2 cut(s) 68, 115
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 3 cut(s) 165, 167, 224
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 2 cut(s) 183, 402
StyI CCWWGG 2 cut(s) 47, 294
TaqI TCGA 1 cut(s) 302
TasI AATT 2 cut(s) 68, 115
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 3 cut(s) 43, 141, 299
Tru1I TTAA 3 cut(s) 389, 453, 469
Tru9I TTAA 3 cut(s) 389, 453, 469
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 4 cut(s) 41, 287, 356, 365
Van91I CCANNNNNTGG 1 cut(s) 419
Vha464I CTTAAG 2 cut(s) 388, 452
VpaK11BI GGWCC 1 cut(s) 203
XceI RCATGY 2 cut(s) 154, 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.