Rroxscaffold_4G00296380

RAVE protein 1 C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003397
Physical Location & Seq
Forward (+)
16436011 .. 16452035
16025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296380.1

Sequence Viewer

Length: 6888 bp
ATGGACCCCACTCACCACCTTCCTCTCCAAATCCTGAGATCCGACCCGACCCCTTCCGCCCCTAACCGATCCGACCCGCCCGGATCCGCCATCGATTGGCTACCGGACTTCCTCGACCTCTCATGGGTCTCCTATGGCGCCTCCTCCCTCCTCGTGATCTCGCACTTCCCTTCTCCGCTCTCCGAAAACGAAACCGTGATCGGTCCCATTTTCCGGCAGGTTTTCGAGCTCTCCGGCGACCCCTCCTCGGCCGTCAGAGCCGTGGCTTGGTCGCCGGTAATGCCGTCTCTCGGTGAGGTTGCGGCCGCGGCTGAGAACTGCGTTTGGGTGTTCTCGCATGATTCGGCGAGCTCCAAAGGTTCTTTTTGTTGGAGCCAGAATGCAGTGCTTGTAAACTCTGCAAAGGTGGAGGCAATTGGATGGACGGGCTCAGGGGATGGAATAATTGCCAGTGGCGTTGAGGTTGTTTTGTGGAAAAGAAGTGGTAGGTCATGGGAAATTGCTTGGAAGTTTAAAGCAGACCAACCCCAGAGTTTGGTCTCTGCAACTTGGTCTGTTGAAGGACCTTTTGCAACTGCTGCTTATCAGAGTAAATGGCTGATTGAAGGATTGTTGACTGAGGAGGCCAGCAAATGTGTGCTAGTAAGCCAGCAAGATGGCAAATCTGAGTTTGTTAAATCTGAGCTACAACATCCTCTTCCTGTCTCAATGATTCAATGGAGGCCATTAACAGGAAAGCCTTTAAGCAGAGATGCAAAACATCCATCAAGGCATGTGCTACTCACTAGTTGCTTAGATGGAACTGTCAGGTTATGGTGCGAGGTTGATGACGGACGGGCAAGAAAAGTTGCTAAGGACATTAATGATCACAAAACCACGAGGTGGTCATTTTCTGTAGCTGCTGTAATTGAGATAAACCAGGCACTGAATGGGATTCTGGGAATTGATATTTATGTGACGTGGGCCATCGAAACTGGGGGTCTAAACAAAGCTAGTGCGGGGGCTAAAAAATTATTGTCTGCAAACGGTTATGAACACGACCAGCTCGGTAATTGCGAGTGGTTAATTGGGTTCGGTCCTGGAATGCTAGTGAAGTTTTGGGCTTTACACTGTCTCGATGATGTCTCCCCAGTAAGATTTCCCCGGGTTACATTATGGAAGACACAAGAACTACAAGTCCTCGAAGGGGGACATGTTCGTAGGACTGGACTTTCAAATTATAATGATAGAATACCACTTAATAAAGTTGTGATCTTGAGGAACTGCATGTCTGGTCCACCAGTGGTCTGCTCTTTGATTCAGTTGTTACCTTGCAATTCATTGGTTTGGTCACTGTTGTATACCCAATCTTCAAATAATGTTGAGGATCTTACCCTCAATAAACCTGGTATGGAGAACACTTTATCATGTTCAGCTGATGGGATCTTGAATTTGGATGGTCATGCTGGAAGAATCTTTCAAGTAGCAGTACATCCTTATAGTTTGCCAACATGGGATATCTGTGGGAAGCTTGCAACTCAAAGTTCATCTTCCAGGTATACAAGCGTGAGGTGGGCACCAGCAATACTAAATGAAGTAGCTGTTCTTCTTATGGGACATGCTGGAGGAATTGATTGCTTTATTGTAAAAATTCATCAGGATGAGGAAGAAATTATAGAATGTCACTACTTATGCACTATACCTTTTACAGGTCATGGTCCTTATGAGGATGGGCCCAACAGTATCTCTACAATTCCTTTGCCTCTGACTTGTCATAAAACTCTAAGATGTAGTAAATTTATGCTTTTGGGTGTATGGATGAATGGGTTTCAAGCTCTTTCATGGGAAATCACTTTACATACTTTTGATTTATCCGGAAGCTACTGTGACTGTGATTTTGAGAGGGGAAATGGTCCTGAAAGTATGTGGGGATTTGAAGGTACTTTCACCAATGTAAGATATTGTCTAAAAGTAAACGCTTGCTCATCGCAGATACCTGATCCATACATCCATGATGAGGTTACAAGTTTTGCTGTGGTCTGTCAGGGTAGTTTGATGCGTATAGAAAAAAATTTGGGTCCTACTATTGCTCAATGCTCTAGTTGTCCTGCTTATTTAATGGCAACAGGCTGCTCTGATGGTTCTTTGAAATTGTGGAGGAGTAGAATTGCTAAACTATCAACTCCTAACATTCCATGGGAACTTGTGGGTATGTTTGTGGCACATAAAGGCCCTATTAGCACTGTATGCTTGTCTGAATGTGGTAGGAAGATTGCAACCATCTGCAAGGATTTTTCTTCAAATACAGTTGGCACTCTTCAGATATGGGATCCTATACATCTTGCAGGTGCAGGGTCATTTGTGTTAGAAGATACAATATCCTTTGATCAAGAACTTGTTGCTTTGAATTGGCTACCTTTGGGAAATGGTCAGTTACTACTGGGAATTTGCATGCTGAATCAGTTTCGAGTATATTCTATTGAGCGTTGTGGTGGTCAGACTTTGTTAAACCCTGAAAAATCAGTGAAAAAGAACATCTGGGTCTGCATTGCCTCTACTCATACTTTCGCTCCCATTTGTGACTTCTTTTGGGGACCCAGAGCAACAGCTATCTTTGTTCATAAGAGTTACTTTTGTGTAAATAGTCAGTGGTTGTTCCTTGTAGATAAGAAACATCTTGCTAATGGTCAGTCAAATGACATAGCAGAAAATTGCATGGATTCTGCAGGTGGAATGAAAGAAGACACTAGTTCAATTTTCTTTGATTGTGAACTTCAACAATTTGATAAAACATTACTTGACGGGAGTAGAAGAGATTGCAAGTCTGGTACACCATTCAAGACTGATTTGAAAAAGGACTATCTATTGAGCAGCTTGTTTGTAGCAAGGTCTCAATTGGATTGTGCTTGGGGTACCAAGCTAGGTTTATGGACAATGCTTGAAGTGGTGGAGAATTTAAGTGGATCTCTGCCAGTTTACCACCCAGAGGCCCTCTTTATGAATATCTATTCAGGAAATTGGAAGCGCGCGTATATTGCATTGAGGCATCTTAATGATTTTCTCTCTTCTGAGTCTTCTTCTGGGAGCAAGCATTACCCTTCAAAGTCAAGCATTTTCGTTCCACAGATTTTGTTGTCAACTTTTCTTGATGGGATTATCTCAATAGATTCAAACGATAAAGGATTTCAATGGAGTGGTGATGCAGTCACTTCATCTTCTCAGTTGCAGAGAGACTCTGGCCAGTTTACATACGACTCGTATGCTTCCAATAATTTGTTCAGTTCATCTTCAACAAAATATGGACTTGTTGACTTTGTTGAGCATCTGGAGAAGTTGTATGAATTAGCAGCTATAACCAACATTGAGAGGATGCAGATTCTTGCTATTTTTGATCTTCTCAATGAAATGACTAATTCGAACTCTGGTTCTGCATATGAAAGTCTCGATGAACCTGGACGGAGGATGCACATTAGAAAGCTTGATGAGATTTTCCAATGGTTGAAAAGAGGTTCAATGATGGAGAAACTAGCAAGGTTGCAATATCTGAAGAGGAGGGATCCTAAGGATTGTGCGTTGCTATATATTGCACTGAATAGAATTCAAGTTCTAACCGGACTTTTCAAAATTAGCAAGGATGAGAAGGACAAGCCCTTAGTCGCATTTCTTTCACGCAATTTTCAGGAAGAGAAAAATAAGGCAGCTGCCTTGAAGAATGCATATGTCTTGATGGGTAGGCATCAACTGGAGTTGGCTGTTGCTTTCTTTCTGCTTGGAGGTGATACTTCTTCTGCTGTCAGTATTTGTGCAAAGAACCTTGGGGATGAACAGCTTGCAGTTGTAATTTGCCGGCTTACTGAAGGGCGTGGTGGACCATTAGAGAGACACCTAATTTCAAAGTCTCTACTACCATTTGCAGTTGAGAGGGGTGACTCCTGGTTAGCAAGCCTTCTGGAGTGGGAGCTAGGGAACTACTGTCAATCTTTTATAAGCATGCTTGGACTCCAGATAAATTCTGCAACTGAGATGTATGCAACTTTGTCCAGTGGTGTTGCTTTTTCAGACCCAAATGTTGGTCTCTATTGTCTATTGCTAGCAACCAAGAACAGCATGAGGAATGCTGTAGGGGAGCGGAATACTGCAATCCTGAGTAGGTGGGCAGTATTCATGACAGCCACTGCCGTAAAGAGATGTGGACTTCCACTTGAAGCTTTGGAGTACCTTTCATCTCCCACAACCATTCTTGGAGACACAGATATAGGGACTGTATCAGATATCGGGGATTTTGAGAAGCTAAATGGAATTCTGAATCCTTCTCCAAAGAATTCTTCAAATTGGTTTTCAAGTAATGTGGCATTCTGTGTGGAGTTCCTTGCCAGATTAGATTTGGCACTTCAATACTTATCAACATTGGTAAGAGAGCATCCAAGTTGGGCAGATACTGTTGTTGCACCTTCCAGAGCTATTTCACATATAAATGAGTGCGAGAATCAGGACCATGTAAAAGTTCTTGGGAGTTTCCACCAGAAATTATACACAGCACTTCATCATTTGGAGCAGAAGTTTTCAGTGGTTCCTTTTCATCTCATCAGCATGGTCTTGCTCTCACTGTACAATCGTGGGCTATGGTTTGTTGGGTACGAAATATTACATGCATATACTTCTCAAGATCAAGATCTCAATAAAAGCCAAATAGTTAGATCCCTATTTATAACTTGCTCTAAACTTAAGTCAGATTGTTTAGAAAACAATGTGTTTGGTGAAAGTGGATCTTCAAGCTCAAACGCTTGGGAGTATTACTTTCAAGGCCTTCAGGGTTCTCAAGGTACAGATGTAGCACATTTAATTCCAGAAGATGAAAGATGGCAGATCACAGGGGCTTGTTTATGGCAACACATATCCAGACTAATGAAACATAAGCTAGTTATGTTATCTTATAAACTTGATGAAGGCTGTGTTTCTGGTGTTCCCGATGGAAAACATTTCTCATCGGTGCCTTGCTCTGAAAATTTGGGTCCTGATGACAACAGAACTGAAGAAGTGATTGGGTTGGTTTCATTGTCCTTGGTTAAATTATTAAAGACCACACTTGCACATGTTTCTTCTTATCATGTGATGCGTCTAGCATCACATCTGCAGCAGAAGATGGATAATGGGTTGCATGTAATGACTCTTGTTTGGTTAGAAGAATATAACCAGTCTCAGACCAGAGGCCTCAATCAGCATTTGAATCAGGACATGCTCAAATTGGAGACGGTAGGTGAAAAACATGGATCAGACATATTATGGGATATTTGTGCAGATCCTAAAATAATATCTGAGAGTTTCACCCAGGAAAAAATAAACTGGTCTCACTCCCTTGATCATAAACCGTCTAAAGGATGGAATAACATATATAGAGGCGTCACAACTGTAGATGAGACTGAGGAAACTCGTAACCATGAACTTACACCTAAGAGTACTTCTGCCAATAGTGAAGCTGGATTACCTTCTAGAAGTCTATTTCGCAGTGGCCACTCTTTCCTTAGCGGATGGCAGAAAGATACAACTGCTACAAAGGAGGTCTCACCTTTCCTGAACCCAAAAGAAATATATAAGAGAAATGGGGAGCTTTTGGAGGCCTTGTGTCTCAACTCTGTTAATCAGAAACAAGCTGCTCTTGCAAGCAACCGAAAGGGCATCATATTTTTTAATTGGAAAGATGATATGCCTGATAGAGATCATTCAGATTATATCTGGTCAGAGGCTGATTGGCCACTGAACGGGTGGGCTGGTTCTGAATCAACACCAGCTCCAACATATGTATCTCCTGCAAGAGACTTGAGAGGTGGAGGAGCATTTGGGAATCAAGGTTATCCTGGAATGCGTGTGTCTGGTTTAGGTTGGGAGACTCGAGAGGACTTTGAGGAAGTTGTTGATACACCACCTACTGTTGAGAATGCAAACACAAGGGTTTTTTCCAGTCACCCATCAAGACCTTTTTTCTTGGTTGGTTCTAGCAATACACACATTTACTTATGGGAGTTTGGCAAAGACAAAGCTACTGCAACTTATGGAGTTCTGCCTGCTGCAAATGTCCCTCCACCTTATGCTCTGGCATCGATATCTGCTTTGCAGTTTGATCACTGCGGACACAGATTTGCTACCGCTGCCTTAGATGGAACTGTATGCACATGGCAGCTGGAGGTGGGAGGAAGGAGTAATATTCGTCCTACAGAATCATCTCTATGCTTTAACAGTCATGCATCGCTTTGTATAATTGTTTATCTTGACATGTCTCACTATTTCGCTGTCCTTGTGGACCTGTGGGATGTTGCTTATGTTACATCAAGTGGATCAATCATTGCTGTGGCTGGATATAGCTCCAGTAGTGTTAATGTGGTCATATGGGATACATTGGCTCCACCTACTACCTCACGAGCTTCCATTATTTGTCATGAAGGTGGGGCGCGCTCTCTTTCTGTTTTCGATAATGACATAGGAAGTGGATCTATCTCACCCCTTATTGTGACTGGTGGTAAAGGTGGTGATGTTGGACTTCACGACTTCCGGTATATTGCTACTGGAAGGAGTAAAAGGCACAGGCACACTGATAAAGGTGAACAAGCTGTGAAGACATCTTCAAATATTGATCATCATCCTGCGGATGGTAACAGATTTGGGGAACAAAATCAGAATGGGATGCTGTGGTATATACCAAAAGCTCACTCAGGGAGCGTCACCAAGATATCCACAATTCCAAATACCAGTTTGTTCTTAACGGGAAGCAAAGATGGAGATGTTAAGCTTTGGGATGCCAAGAGAGCTAAGTTGGCTGCTGTCACCGACATAAAAGTTGTTTCACAAGGTTTTCTTACATGTGGTGGAGATGGAACTGTAAAGCTGGTTCACCTCAAAGACCACCAATATTAG

Protein Analysis

2295

Amino Acids

253.7

Weight (kDa)

6.05

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rav1p_C PF12234 1155 - 1325 7.5e-48 RAVE protein 1 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1221, 3965, 4688, 4914
AarI CACCTGC 2 cut(s) 2315, 2696
Acc36I ACCTGC 3 cut(s) 208, 2315, 2696
Acc65I GGTACC 1 cut(s) 2891
AccB1I GGYRCC 4 cut(s) 137, 1555, 2891, 4969
AccB7I CCANNNNNTGG 4 cut(s) 96, 535, 882, 4049
AccBSI CCGCTC 2 cut(s) 178, 4108
AccI GTMKAC 2 cut(s) 1340, 1538
AccII CGCG 4 cut(s) 308, 3006, 3008, 6427
AccIII TCCGGA 1 cut(s) 1853
AcoI YGGCCR 5 cut(s) 249, 303, 3217, 5488, 5729
AcuI CTGAAG 5 cut(s) 2282, 3545, 3855, 4772, 5031
AcyI GRCGYC 2 cut(s) 138, 5379
AdeI CACNNNGTG 1 cut(s) 882
AfaI GTAC 9 cut(s) 1470, 1921, 2809, 2893, 4196, 4589, 4616, 4804, 5437
AflII CTTAAG 1 cut(s) 4703
AflIII ACRYGT 4 cut(s) 1192, 5071, 6249, 6833
AhlI ACTAGT 2 cut(s) 785, 2726
AjiI CACGTC 1 cut(s) 960
AjnI CCWGG 8 cut(s) 918, 1078, 1384, 1532, 3430, 3911, 5307, 5833
AjuI GAANNNNNNNTTGG 6 cut(s) 3239, 3271, 4288, 4320, 5004, 5036
AloI GAACNNNNNNTCC 2 cut(s) 1161, 1193
Alw21I GWGCWC 2 cut(s) 231, 353
AlwNI CAGNNNCTG 4 cut(s) 925, 4154, 4418, 5723
Ama87I CYCGRG 2 cut(s) 1143, 5868
Aor13HI TCCGGA 1 cut(s) 1853
ApaI GGGCCC 1 cut(s) 1716
ArsI GACNNNNNNTTYG 2 cut(s) 1859, 1891
AseI ATTAAT 1 cut(s) 861
Asp718I GGTACC 1 cut(s) 2891
AspLEI GCGC 5 cut(s) 140, 3006, 3008, 6427, 6429
AsuC2I CCSGG 3 cut(s) 81, 1144, 1145
AsuII TTCGAA 1 cut(s) 3395
AsuNHI GCTAGC 1 cut(s) 4069
AvaI CYCGRG 2 cut(s) 1143, 5868
AxyI CCTNAGG 1 cut(s) 3540
BaeGI GKGCMC 2 cut(s) 1558, 1716
BaeI ACNNNNGTAYC 6 cut(s) 4407, 4440, 4794, 4827, 5763, 5796
BalI TGGCCA 3 cut(s) 3219, 5490, 5731
BamHI GGATCC 3 cut(s) 83, 2308, 3535
BanI GGYRCC 4 cut(s) 137, 1555, 2891, 4969
BanII GRGCYC 4 cut(s) 231, 353, 431, 1716
BarI GAAGNNNNNNTAC 4 cut(s) 1569, 1601, 5445, 5477
BauI CACGAG 3 cut(s) 152, 877, 6393
BbsI GAAGAC 4 cut(s) 1166, 2727, 3045, 6596
Bbv12I GWGCWC 2 cut(s) 231, 353
BceAI ACGGC 4 cut(s) 236, 245, 268, 4142
BcgI CGANNNNNNTGC 2 cut(s) 3221, 3255
BciT130I CCWGG 8 cut(s) 920, 1080, 1386, 1534, 3432, 3913, 5309, 5835
BciVI GTATCC 1 cut(s) 6361
BclI TGATCA 5 cut(s) 865, 2365, 5338, 6097, 6607
BcnI CCSGG 3 cut(s) 81, 1144, 1145
BcuI ACTAGT 2 cut(s) 785, 2726
BfmI CTRYAG 6 cut(s) 894, 2703, 4098, 5111, 5388, 6189
BfoI RGCGCY 1 cut(s) 141
BfrI CTTAAG 1 cut(s) 4703
BfuAI ACCTGC 3 cut(s) 208, 2315, 2696
BfuI GTATCC 1 cut(s) 6361
BglII AGATCT 1 cut(s) 4651
BmcAI AGTACT 1 cut(s) 5437
BmeT110I CYCGRG 2 cut(s) 1143, 5868
BmgBI CACGTC 1 cut(s) 960
BmrI ACTGGG 3 cut(s) 984, 1124, 2429
BmtI GCTAGC 1 cut(s) 4073
BmuI ACTGGG 3 cut(s) 984, 1124, 2429
BpiI GAAGAC 4 cut(s) 1166, 2727, 3045, 6596
BpmI CTGGAG 7 cut(s) 1623, 3326, 3743, 3950, 3965, 6179, 6325
Bpu10I CCTNAGC 3 cut(s) 430, 852, 5501
Bpu14I TTCGAA 1 cut(s) 3395
BpuEI CTTGAG 4 cut(s) 1276, 4626, 4782, 5818
BpuMI CCSGG 3 cut(s) 81, 1144, 1145
Bsa29I ATCGAT 2 cut(s) 93, 6077
BsaBI GATNNNNATC 3 cut(s) 4650, 5694, 6612
BsaHI GRCGYC 2 cut(s) 138, 5379
BsaI GGTCTC 6 cut(s) 133, 544, 2874, 4058, 5331, 5545
BsaJI CCNNGG 9 cut(s) 246, 261, 306, 1142, 1143, 2174, 3793, 5040, 5307
BsaWI WCCGGW 4 cut(s) 103, 1853, 3590, 6525
Bse118I RCCGGY 2 cut(s) 274, 3825
Bse21I CCTNAGG 1 cut(s) 3540
Bse3DI GCAATG 2 cut(s) 2526, 6318
Bse8I GATNNNNATC 3 cut(s) 4650, 5694, 6612
BseAI TCCGGA 1 cut(s) 1853
BseBI CCWGG 8 cut(s) 920, 1080, 1386, 1534, 3432, 3913, 5309, 5835
BseCI ATCGAT 2 cut(s) 93, 6077
BseDI CCNNGG 9 cut(s) 246, 261, 306, 1142, 1143, 2174, 3793, 5040, 5307
BseJI GATNNNNATC 3 cut(s) 4650, 5694, 6612
BseMI GCAATG 2 cut(s) 2526, 6318
BsePI GCGCGC 2 cut(s) 3004, 6425
BseRI GAGGAG 7 cut(s) 133, 140, 235, 635, 2152, 3544, 5823
BseSI GKGCMC 2 cut(s) 1558, 1716
BseX3I CGGCCG 2 cut(s) 249, 303
BsgI GTGCAG 2 cut(s) 2349, 5295
Bsh1236I CGCG 4 cut(s) 308, 3006, 3008, 6427
Bsh1285I CGRYCG 2 cut(s) 252, 306
BshNI GGYRCC 4 cut(s) 137, 1555, 2891, 4969
BshVI ATCGAT 2 cut(s) 93, 6077
BsiEI CGRYCG 2 cut(s) 252, 306
BsiHKAI GWGCWC 2 cut(s) 231, 353
BsiHKCI CYCGRG 2 cut(s) 1143, 5868
BslFI GGGAC 6 cut(s) 189, 1203, 1608, 2586, 4252, 6038
BsmBI CGTCTC 2 cut(s) 291, 5222
BsmFI GGGAC 6 cut(s) 189, 1203, 1608, 2586, 4252, 6038
BsmI GAATGC 7 cut(s) 385, 1089, 3697, 4099, 4331, 5844, 5920
Bso31I GGTCTC 6 cut(s) 133, 544, 2874, 4058, 5331, 5545
BsoBI CYCGRG 2 cut(s) 1143, 5868
Bsp119I TTCGAA 1 cut(s) 3395
Bsp120I GGGCCC 1 cut(s) 1712
Bsp1286I GDGCHC 5 cut(s) 231, 353, 431, 1558, 1716
Bsp13I TCCGGA 1 cut(s) 1853
Bsp1407I TGTACA 1 cut(s) 4587
Bsp19I CCATGG 1 cut(s) 2174
BspDI ATCGAT 2 cut(s) 93, 6077
BspEI TCCGGA 1 cut(s) 1853
BspFNI CGCG 4 cut(s) 308, 3006, 3008, 6427
BspHI TCATGA 2 cut(s) 4143, 6412
BspMAI CTGCAG 2 cut(s) 2707, 5115
BspMI ACCTGC 3 cut(s) 208, 2315, 2696
BspOI GCTAGC 1 cut(s) 4073
BspT104I TTCGAA 1 cut(s) 3395
BspT107I GGYRCC 4 cut(s) 137, 1555, 2891, 4969
BspTI CTTAAG 1 cut(s) 4703
BspTNI GGTCTC 6 cut(s) 133, 544, 2874, 4058, 5331, 5545
BsrBI CCGCTC 2 cut(s) 178, 4108
BsrDI GCAATG 2 cut(s) 2526, 6318
BsrFI RCCGGY 2 cut(s) 274, 3825
BsrGI TGTACA 1 cut(s) 4587
BssAI RCCGGY 2 cut(s) 274, 3825
BssECI CCNNGG 9 cut(s) 246, 261, 306, 1142, 1143, 2174, 3793, 5040, 5307
BssHII GCGCGC 2 cut(s) 3004, 6425
BssNAI GTATAC 2 cut(s) 1341, 1539
BssNI GRCGYC 2 cut(s) 138, 5379
BssSI CACGAG 3 cut(s) 152, 877, 6393
BssT1I CCWWGG 3 cut(s) 2174, 3793, 5040
Bst1107I GTATAC 2 cut(s) 1341, 1539
Bst2BI CACGAG 3 cut(s) 152, 877, 6393
Bst2UI CCWGG 8 cut(s) 920, 1080, 1386, 1534, 3432, 3913, 5309, 5835
Bst6I CTCTTC 6 cut(s) 702, 2301, 2785, 3049, 3521, 3657
BstACI GRCGYC 2 cut(s) 138, 5379
BstAFI CTTAAG 1 cut(s) 4703
BstAPI GCANNNNNTGC 2 cut(s) 578, 2226
BstAUI TGTACA 1 cut(s) 4587
BstBI TTCGAA 1 cut(s) 3395
BstDSI CCRYGG 3 cut(s) 261, 306, 2174
BstENI CCTNNNNNAGG 1 cut(s) 1686
BstFNI CGCG 4 cut(s) 308, 3006, 3008, 6427
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 5 cut(s) 140, 3006, 3008, 6427, 6429
BstMCI CGRYCG 2 cut(s) 252, 306
BstNI CCWGG 8 cut(s) 920, 1080, 1386, 1534, 3432, 3913, 5309, 5835
BstSFI CTRYAG 6 cut(s) 894, 2703, 4098, 5111, 5388, 6189
BstSLI GKGCMC 2 cut(s) 1558, 1716
BstUI CGCG 4 cut(s) 308, 3006, 3008, 6427
BstV2I GAAGAC 4 cut(s) 1166, 2727, 3045, 6596
BstXI CCANNNNNNTGG 1 cut(s) 656
BstZ17I GTATAC 2 cut(s) 1341, 1539
BstZI CGGCCG 2 cut(s) 249, 303
Bsu15I ATCGAT 2 cut(s) 93, 6077
Bsu36I CCTNAGG 1 cut(s) 3540
BsuI GTATCC 1 cut(s) 6361
BsuTUI ATCGAT 2 cut(s) 93, 6077
BtgI CCRYGG 3 cut(s) 261, 306, 2174
BtgZI GCGATG 2 cut(s) 1950, 6207
BtrI CACGTC 1 cut(s) 960
BtsI GCAGTG 4 cut(s) 390, 4152, 5491, 6100
BveI ACCTGC 3 cut(s) 208, 2315, 2696
CaiI CAGNNNCTG 4 cut(s) 925, 4154, 4418, 5723
CciI TCATGA 2 cut(s) 4143, 6412
CciNI GCGGCCGC 1 cut(s) 303
CfoI GCGC 5 cut(s) 140, 3006, 3008, 6427, 6429
Cfr10I RCCGGY 2 cut(s) 274, 3825
Cfr42I CCGCGG 1 cut(s) 309
Cfr9I CCCGGG 1 cut(s) 1143
ClaI ATCGAT 2 cut(s) 93, 6077
CseI GACGC 3 cut(s) 5084, 5368, 6682
CsiI ACCWGGT 1 cut(s) 1384
Csp6I GTAC 9 cut(s) 1469, 1920, 2808, 2892, 4195, 4588, 4615, 4803, 5436
CspCI CAANNNNNGTGG 4 cut(s) 3090, 3125, 4308, 4343
CviQI GTAC 9 cut(s) 1469, 1920, 2808, 2892, 4195, 4588, 4615, 4803, 5436
DinI GGCGCC 1 cut(s) 139
DraI TTTAAA 1 cut(s) 514
DraIII CACNNNGTG 1 cut(s) 882
EaeI YGGCCR 5 cut(s) 249, 303, 3217, 5488, 5729
EagI CGGCCG 2 cut(s) 249, 303
Eam1104I CTCTTC 6 cut(s) 702, 2301, 2785, 3049, 3521, 3657
EarI CTCTTC 6 cut(s) 702, 2301, 2785, 3049, 3521, 3657
EciI GGCGGA 2 cut(s) 46, 76
Ecl136II GAGCTC 2 cut(s) 229, 351
EclXI CGGCCG 2 cut(s) 249, 303
Eco130I CCWWGG 3 cut(s) 2174, 3793, 5040
Eco147I AGGCCT 3 cut(s) 4785, 5190, 5597
Eco24I GRGCYC 4 cut(s) 231, 353, 431, 1716
Eco31I GGTCTC 6 cut(s) 133, 544, 2874, 4058, 5331, 5545
Eco32I GATATC 4 cut(s) 1498, 4252, 6081, 6705
Eco52I CGGCCG 2 cut(s) 249, 303
Eco53kI GAGCTC 2 cut(s) 229, 351
Eco57I CTGAAG 5 cut(s) 2282, 3545, 3855, 4772, 5031
Eco81I CCTNAGG 1 cut(s) 3540
Eco88I CYCGRG 2 cut(s) 1143, 5868
EcoICRI GAGCTC 2 cut(s) 229, 351
EcoNI CCTNNNNNAGG 1 cut(s) 1686
EcoO109I RGGNCCY 6 cut(s) 563, 2057, 2210, 2573, 2968, 4991
EcoRI GAATTC 3 cut(s) 3576, 4278, 4300
EcoRII CCWGG 8 cut(s) 918, 1078, 1384, 1532, 3430, 3911, 5307, 5833
EcoRV GATATC 4 cut(s) 1498, 4252, 6081, 6705
EcoT14I CCWWGG 3 cut(s) 2174, 3793, 5040
EcoT22I ATGCAT 3 cut(s) 3697, 4633, 6223
EcoT38I GRGCYC 4 cut(s) 231, 353, 431, 1716
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
ErhI CCWWGG 3 cut(s) 2174, 3793, 5040
Esp3I CGTCTC 2 cut(s) 291, 5222
FaqI GGGAC 6 cut(s) 189, 1203, 1608, 2586, 4252, 6038
FauI CCCGC 2 cut(s) 84, 991
FauNDI CATATG 4 cut(s) 3412, 3697, 5776, 6362
FbaI TGATCA 5 cut(s) 865, 2365, 5338, 6097, 6607
FblI GTMKAC 2 cut(s) 1340, 1538
FriOI GRGCYC 4 cut(s) 231, 353, 431, 1716
GlaI GCGC 5 cut(s) 139, 3005, 3007, 6426, 6428
GsuI CTGGAG 7 cut(s) 1623, 3326, 3743, 3950, 3965, 6179, 6325
HaeII RGCGCY 1 cut(s) 141
HgaI GACGC 3 cut(s) 5084, 5368, 6682
HhaI GCGC 5 cut(s) 140, 3006, 3008, 6427, 6429
Hin1I GRCGYC 2 cut(s) 138, 5379
Hin6I GCGC 5 cut(s) 138, 3004, 3006, 6425, 6427
HinP1I GCGC 5 cut(s) 138, 3004, 3006, 6425, 6427
HincII GTYRAC 3 cut(s) 615, 3117, 3289
HindII GTYRAC 3 cut(s) 615, 3117, 3289
HindIII AAGCTT 4 cut(s) 1508, 3455, 4185, 6761
HpyCH4IV ACGT 1 cut(s) 959
HpySE526I ACGT 1 cut(s) 959
Hsp92I GRCGYC 2 cut(s) 138, 5379
HspAI GCGC 5 cut(s) 138, 3004, 3006, 6425, 6427
KasI GGCGCC 1 cut(s) 137
KflI GGGWCCC 1 cut(s) 2573
Kpn2I TCCGGA 1 cut(s) 1853
KpnI GGTACC 1 cut(s) 2895
KroI GCCGGC 1 cut(s) 3825
KroNI GCCGGC 1 cut(s) 3827
Ksp22I TGATCA 5 cut(s) 865, 2365, 5338, 6097, 6607
KspI CCGCGG 1 cut(s) 309
MabI ACCWGGT 1 cut(s) 1384
MaeII ACGT 1 cut(s) 959
MbiI CCGCTC 2 cut(s) 178, 4108
MfeI CAATTG 2 cut(s) 414, 2873
MhlI GDGCHC 5 cut(s) 231, 353, 431, 1558, 1716
MlsI TGGCCA 3 cut(s) 3219, 5490, 5731
MluNI TGGCCA 3 cut(s) 3219, 5490, 5731
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 7 cut(s) 3059, 3206, 3227, 3902, 3972, 5140, 5860
MmeI TCCRAC 5 cut(s) 66, 96, 350, 5795, 6490
Mox20I TGGCCA 3 cut(s) 3219, 5490, 5731
Mph1103I ATGCAT 3 cut(s) 3697, 4633, 6223
MroI TCCGGA 1 cut(s) 1853
MroNI GCCGGC 1 cut(s) 3825
MscI TGGCCA 3 cut(s) 3219, 5490, 5731
MslI CAYNNNNRTG 7 cut(s) 1638, 3030, 4452, 4568, 6202, 6323, 6417
Msp20I TGGCCA 3 cut(s) 3219, 5490, 5731
MspA1I CMGCKG 5 cut(s) 308, 1415, 3680, 6125, 6157
MspCI CTTAAG 1 cut(s) 4703
MunI CAATTG 2 cut(s) 414, 2873
Mva1269I GAATGC 7 cut(s) 385, 1089, 3697, 4099, 4331, 5844, 5920
MvaI CCWGG 8 cut(s) 920, 1080, 1386, 1534, 3432, 3913, 5309, 5835
MvnI CGCG 4 cut(s) 308, 3006, 3008, 6427
NaeI GCCGGC 1 cut(s) 3827
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 3 cut(s) 81, 1144, 1145
NcoI CCATGG 1 cut(s) 2174
NdeI CATATG 4 cut(s) 3412, 3697, 5776, 6362
NgoMIV GCCGGC 1 cut(s) 3825
NheI GCTAGC 1 cut(s) 4069
NmeAIII GCCGAG 1 cut(s) 227
NotI GCGGCCGC 1 cut(s) 303
NsiI ATGCAT 3 cut(s) 3697, 4633, 6223
NspV TTCGAA 1 cut(s) 3395
PaeI GCATGC 2 cut(s) 2434, 3973
PaeR7I CTCGAG 1 cut(s) 5868
PagI TCATGA 2 cut(s) 4143, 6412
PaqCI CACCTGC 2 cut(s) 2315, 2696
PauI GCGCGC 2 cut(s) 3004, 6425
PceI AGGCCT 3 cut(s) 4785, 5190, 5597
PciI ACATGT 4 cut(s) 1192, 5071, 6249, 6833
PcsI WCGNNNNNNNCGW 1 cut(s) 1053
PctI GAATGC 7 cut(s) 385, 1089, 3697, 4099, 4331, 5844, 5920
PdiI GCCGGC 1 cut(s) 3827
PflMI CCANNNNNTGG 4 cut(s) 96, 535, 882, 4049
PfoI TCCNGGA 2 cut(s) 1078, 5833
PleI GAGTC 7 cut(s) 3058, 3206, 3227, 3902, 3972, 5140, 5860
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 7 cut(s) 3058, 3206, 3227, 3902, 3972, 5140, 5860
PpuMI RGGWCCY 4 cut(s) 563, 2057, 2573, 4991
PscI ACATGT 4 cut(s) 1192, 5071, 6249, 6833
PshBI ATTAAT 1 cut(s) 861
PsiI TTATAA 4 cut(s) 1221, 3965, 4688, 4914
Psp124BI GAGCTC 2 cut(s) 231, 353
Psp5II RGGWCCY 4 cut(s) 563, 2057, 2573, 4991
Psp6I CCWGG 8 cut(s) 918, 1078, 1384, 1532, 3430, 3911, 5307, 5833
PspGI CCWGG 8 cut(s) 918, 1078, 1384, 1532, 3430, 3911, 5307, 5833
PspOMI GGGCCC 1 cut(s) 1712
PspPPI RGGWCCY 4 cut(s) 563, 2057, 2573, 4991
PsrI GAACNNNNNNTAC 2 cut(s) 6846, 6878
PstI CTGCAG 2 cut(s) 2707, 5115
PstNI CAGNNNCTG 4 cut(s) 925, 4154, 4418, 5723
PteI GCGCGC 2 cut(s) 3004, 6425
PvuII CAGCTG 3 cut(s) 1415, 3680, 6157
RsaI GTAC 9 cut(s) 1470, 1921, 2809, 2893, 4196, 4589, 4616, 4804, 5437
RsaNI GTAC 9 cut(s) 1469, 1920, 2808, 2892, 4195, 4588, 4615, 4803, 5436
RseI CAYNNNNRTG 7 cut(s) 1638, 3030, 4452, 4568, 6202, 6323, 6417
SacI GAGCTC 2 cut(s) 231, 353
SacII CCGCGG 1 cut(s) 309
ScaI AGTACT 1 cut(s) 5437
SchI GAGTC 7 cut(s) 3059, 3206, 3227, 3902, 3972, 5140, 5860
SduI GDGCHC 5 cut(s) 231, 353, 431, 1558, 1716
SexAI ACCWGGT 1 cut(s) 1384
SfcI CTRYAG 6 cut(s) 894, 2703, 4098, 5111, 5388, 6189
SfoI GGCGCC 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 5868
Sfr303I CCGCGG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 3395
SgrBI CCGCGG 1 cut(s) 309
SlaI CTCGAG 1 cut(s) 5868
SmaI CCCGGG 1 cut(s) 1145
SmiMI CAYNNNNRTG 7 cut(s) 1638, 3030, 4452, 4568, 6202, 6323, 6417
SmlI CTYRAG 6 cut(s) 1255, 4641, 4703, 4797, 5797, 5868
SmoI CTYRAG 6 cut(s) 1255, 4641, 4703, 4797, 5797, 5868
SpeI ACTAGT 2 cut(s) 785, 2726
SphI GCATGC 2 cut(s) 2434, 3973
SseBI AGGCCT 3 cut(s) 4785, 5190, 5597
SspDI GGCGCC 1 cut(s) 137
SspI AATATT 4 cut(s) 4623, 6181, 6604, 6884
SstI GAGCTC 2 cut(s) 231, 353
StuI AGGCCT 3 cut(s) 4785, 5190, 5597
StyI CCWWGG 3 cut(s) 2174, 3793, 5040
TaiI ACGT 1 cut(s) 962
TaqII GACCGA 2 cut(s) 191, 1064
TatI WGTACW 3 cut(s) 1468, 4587, 5435
TauI GCSGC 3 cut(s) 305, 308, 311
TspGWI ACGGA 2 cut(s) 846, 3451
TspMI CCCGGG 1 cut(s) 1143
Van91I CCANNNNNTGG 4 cut(s) 96, 535, 882, 4049
Vha464I CTTAAG 1 cut(s) 4703
VspI ATTAAT 1 cut(s) 861
XagI CCTNNNNNAGG 1 cut(s) 1686
XbaI TCTAGA 1 cut(s) 5468
XcmI CCANNNNNNNNNTGG 3 cut(s) 926, 4360, 5739
XhoI CTCGAG 1 cut(s) 5868
XmaI CCCGGG 1 cut(s) 1143
XmiI GTMKAC 2 cut(s) 1340, 1538
ZrmI AGTACT 1 cut(s) 5437
Zsp2I ATGCAT 3 cut(s) 3697, 4633, 6223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.