Rh1CG272000

RAVE protein 1 C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003397
Physical Location & Seq
Reverse (-)
53862275 .. 53876553
14279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG272000.1

Sequence Viewer

Length: 7461 bp
ATGGTAGACTGGGACACACCCACCTATCATCTACTTCCTCCAACTCAAACTCACCATTCCGGACTCTCCCCTATCACCATGGACCCCACTCCCCATCTTCCTCTCCAGATCCTTAGATCGGACCCGACCCCTTCCGCCCCTAACCGATCCGACCCGCCCGGATCCGCCATCGACTGGCTCCCGGACTTCCTCGACCTCTCATGGGTCTCCTACGGCGCCTCCTCCCTCCTCGTCATCTCCCACTTCCCTTCTCGGCTCTCCGAAAACGAAACCGTGATCGGTCCCATTTTCCGGCAGGTTTTCGAGCTCTCCGGCGACCCCTCCTCGGCCGTCAGAGCCGTGTCTTGGTCGCCGGTCATGCCGTCTCTCGGCGAGGTTGCGGCCGCGGCTGAGAATTGCGTTTGGGTGTTCTCGCATGATTCGGCGAGCTCCAAAGGTTCTTTTTGTTGGAGCCAGAATGCAGTGCTTGTAAACTCTGCAAAGGTGGAGGCAATTGGATGGACGGGCTCAGGGGATGGAATAATTGCCAGTGGCGTTGAGGTTGTTCTGTGGAAAAGAAGTGGTAGGTCATGGGAAATTGCTTGGAAGTTTAAAGCAGACCAACCCCAGAGTTTGGTCTCTGCAACTTGGTCTGTTGAAGGACCTTTTGCAACTGCTGCTTATCAGAGTAAATGGCTGATTGAAGGTTTGTTGACTAAGGAGGCTAGCAAATGTGTGCTAGTAAGCCAGCGAGATGGCAAATCTGAGTTTGTTAAATCTGAGCTACGACATCCTCTTCCTGTCTCAATGATTCAGTGGAGGCCATTAACTGGAAAACCTTTAAGCAGAGATGCAAAACATCCATCAAGGCATGTGCTACTCACTAGTTGCTTAGATGGAACTGTCAGGTTATGGTGCGAGGTTGATGACGGACGGGCAAGAAAAGTTGCTAAGGACATCAATGATCACAAAACCACGAGGTGGTCATTTTCTGTAGCCGCTGTAATTGAGATAAACCAGGCACTGAATGGGATTCTGGGAATTGATATTTATGTGACGTGGGCCATAGAAACTGGGGGTCTAAACAAAGCTAGTGTGGGGGCTAAAAAATTATTCTCTGCAAACGGTTATGAACATGACCAGCTCGGTAATTGTGAGTGGTTAGTTGGGTTCGGTCCTGGAATGCTAGTGAAGTTTTGGGCTTTACACTGTCTCGATGATGTCTCCCCAGTAAGATTTCCCCGGGTTACATTATGGAAGACACAAGAACTACAAGTCCTGGAAGGGGGACATGTTCATAGGACTGGACTTTCAAATTATAATGATAGAATACCACTTAATAAAGTTGTGATCTTGAGGAACTGCATGTCTGGTCCACCAGTGGTCTGCTCTTTGATTCAGTTGTTACCTTGCAATTCATTGGTTTGGTCACTGTTGTATACTCAATCTTCAAATAATGTAGAGGATCTTACCCTCAATAAACCTGGTATGGAGAACACTTTATCATGCTCAGCTGATGGGATCTTGAATTTGGATGGTCATGCTGGAAGAATCTTACAAGTAGCAGTGCATCCTTATAGTTGTGAGGTTGAACTTGCTGTCTCCTTAGATTCTGATGGATTGCTTCTTTTTTGGTTTTTCTCTACCATTTCCAACTATATTTTGGGCCGTCCAACACTAGTGCCAACATGGGATATCTGTGGGAAGCTTGCAACTCAAAGTTCATCTTCCAGGTATTCAAGCGTGAGGTGGGCACCAGCAATACTAAATGAAGTAGCTGTTCTTCTTATGGGACATGCTGGAGGAATTGATTGCTTTATTGTAAAAATTCATCAGGATGAGGAAGAAATTATAGAATGTCACTACTTATGCACTATACCTTTTACAGGTCATGGTCCTTATGAGGATGGGCCCAACAGTATCTCTACAATTCCTTTTCCTCCGACTTGTCATAAAACTCTAAGATGTAGTAAATTTATGCTTTTGGGTGTATGGATGAATGGGTTTCAAGCTCTTTCATGGGAAATCACTTTACATACTTTTGATTTATCCGGAAGCTACTGTGACTGTGATTTTGAGACGGGAAATGGTCCTGAAAGTATGTGGGGATTTGAAGGTACTTTCACCAATGTAAGATATTGTCTAAAAGTAAACGCTTGCTCATCGCAGATACCTGATCCATACATCCATGATGAGGTTACAAGTTTTGCTGTGGTCTGTCAGGGTAGTTTGATGTGTATAGAAAAAAATTTGGGTCCTACTATTGATCAATGCTCTAGTTGCCCTGCTTATTTAATGGCTACAGGCTGCTCTGATGGTTCTTTGAAATTGTGGAGGAGTAGAATTGCTAAACTATCAACTCCTAACATTCCATGGGAACTTGTGGGTATGTTTGTGGCACATAAAGGCCCTATTAGCACTGTATGCTTGTCTGAATGTGGTAGGAAGATTGCAACCATCTGCAAGGATTTTTCTTCAAATGCAGTTGGCACTCTTCGGATATGGGATCCTATACATCTTGCTGGTGCAGGGTCATTTGTGTTAGAAGATACAATATCCTTTGATCAAGAACTTGTTGCTTTGAATTGGCTTCCTTTGGGAAATGGTCAGTTACTACTGGGAGTTTGCATGCTGAATCAGTTTCAAGTATATTCTCTTGAGCGTTGTGGTGGTCAGACTTTGTTAAACCCTGAAAAATCAGTGAAAAAGAACATCTGGGTCTGCATTGCTTCTACTCATACTTTCCCTCCCATTTGTGACTTCTTTTGGGGACCCAGAGCAACAGCTGTCTTTGTTCATAAGAGTTACTTTTGTGTAAATAGTCAGTGGTTGTTCCTTGTAGATAAGAAACATCTTGCTAATGGTCAGTCAAATGACATGGCAAAAAATTGCATGGATTCTGCAGGTGGAATGAAAGAAGACACTAGTTCGATTTTCTTTGATTGTGAACTTCAACAATTTGATAAAACATTACTTGACGAGAGTAGAAGAGATTGCAAGTCTGGTACACCATTCAAGACTGATTTGAAAAAGGACTATCTATTGAGCAGCTTGTTTGTAGCAAGGTCTCAATTGGATTGTGCTTGGGGTACCAAGCTAGGTTTATGGACAATGCTTGAAGTGGTGGAGAATTTAAGTGGATCTCTGCCAGTTTACCACCCAGAGGCCCTCTTTATGAATATCTATTCAGGAAATTGGAAGCGCGCGTATATTGCATTGAGGCATCTCAATGATTTTCTCTCTTCTGAGTCTTCTTCTGGGAGCAAGCATTACCCTTCAAAGTCGAGCATTTTCGTTCCACAGATTTTGTTGTCAACTTTTCTTGATGGGATTATCTCAATAGATTCAAACGATAAAGGATTTCAATGGAGTGGTGATGCAGTCACTTCATCTTCTCAGTTGCAGAGAGACTCTGGTCAGTTTACATATGACTCGTATGCTTCCAATAATTTGTTCAGTTCATCTTCAACAAAGTATGGACTTGTTGACTTTGTTGAGCATCTGGAGAAGTTGTATGAATTAGCAGCTATAACCAACATTGAGAGGATGCAGATTCTTGCTATTTTTGATCTTCTTAATGAAATGACTAATTCAAACTCTGGATCTGCGTATGAAAGTCTCGATGAACCTGGACAGAGGTTCTGGATTGCATTGAGGTTTCAGCAACTACATTTTGTCCGGAAGTTTGGTAGATCAGTATCCGTGGAGGAATTGGTTGTGGACTCTAAGTTGATTGGATGGGCCTACCACTCTGATTGCCAGGAAAATTTGTTTGGATCTTTCCTACCCAATGAACCGTCTTGGCAAGAAATGCGGAATTTGGGTGTAGGATTTTGGTTTACAAATACAGCACAATTGCGTTCAAGGATGGAGAAACTAGCAAGGTTGCAATATCTGAAGAGGAGGGATCCTAAGGATTGTGCATTGCTATATATTGCACTGAATAGAATTCAAGTTCTGACCGGACTTTTCAAAATTAGCAAGGATGAGAAGGACAAGCCCTTAGTCGCATTTCTTTCACGCAATTTTCAGGAAGAGAAAAATAAGGCAGCTGCCTTGAAGAACGCATATGTCTTGATGGGTAGGCATCAACTGGAGTTGGCTGTTGCTTTCTTTCTGCTTGGAGGTGATACTTCTTCTGCTGTCAGTATTTGTGCAAAGAACCTTGGGGATGAACAGCTTGCAGTTGTAATTTGCCGGCTTACTGAAGGGCGTGGTGGACCATTAGAGAGACACCTAATTTCAAAGTCTCTACTACCATTTGCAATTGAGAGGGGTGACTCCTGGTTAGCAAGCCTTCTGGAGTGGGAGCTAGGGAACTACTGTCAATCTTTTATAAGCATGCTTGGACTCCAGATAAATTCTGCAACTGAGATGTATGCAACTTTGTCCAGTGGTGTTACTTTTTCAGACCCAAATGTTGGTCTCTATTGTATATTGCTAGCAACCAAGAACAGCATGAGGAATGCTGTAGGGGAGCGGAATACTGCAATCCTGAGTAGGTGGGCATTATTCATGACAGCCACTGCCTTAAAGAGATGTGGACTTCCACTTGAAGCTTTGGAGTACCTTTCATCTCCCACAACCATTCTTGGAGACACAGATCTAGGGACTGTATCAGATATTGGGGATTTTGAGAAGCTAAATGGAATTCTGAATCCTTCTCCAAATAATTCTTCAAATTGGTTATCAAGTACTGTGGCATTCCGTGTGGAGTTCCTTGCCAGATTAGATTTGGCACTTCAATACTTATCAACATTGGTAAGAGAGCATCCAAGTTGGGCAGATACTGTTGTTGCACCTTCCAGAGCTATTTCACATATCAATGAGTGCGAGAATCATGAACATGTAAAAGTTCTTGGGAGTTTCCGCCAGAAATTATACACAGCACTTCATCATTTGGAGCAGAAGTTTTCAGTGGTTCCTTTTCATCTCATCAGCATGGTCTTGCTCTCACTGTACAATCGTGGGCTATGGTTTGTTGGGTACGAAATATTACATTCATATACTTCTCAAGATCAAGATCTCAATGAAAGCCAAATAGTTAGATCCCTATTGCATCCTCTTATGCATAAGCAACTTTTGAAGGCTACCAGAGAAACTTCTCTTTTATTTTCACGCGTTATTGCTGCCTGCAGTATAACTTGCTCTAAACTGAAGTCAGATTGTTTGGAAAACAATGTGTCTGGTGAAAGTGGATCTTCAAGCTCAAACGCTTGGGAGTATTACTTTCAAGGTCTTATCCTGTCACTTCGGAGTTTAAGGAGTGCTTTGCAAATTATTTCTGTCTCATCTACTGAAGACCTCATCATGAAACCCCTTATCATCATTGATTGGATTGAGTATTATGTACAGTTTGCTTGTGCATGGCTTCAAAAAAACTCAAAAGTTCTCTTCCTATTAATGCAACCACTTCTGATCACATTTACCAATGGGCATACACCTTATGAAGTTGATTTGATGGATCTGAAGAAAATCCTCCCCCAGATTGCAGAATCGGTGACCCAAAATTCTTTGATTGATAATGTATGCACAGGCCTTCAGGATTCTCAAGGTACAGATGTAGCACATTTAATTCCAGAAGATGAAAGATGGCAGATCACAGGGGCTTGTTTATGGCAACACATATCCAGACTAATGAAACATAAGATAGGTATGTTATCTTATAAACTTGATGAAGGCTGTATTTCTGGTGTTCCCGATGGAAAACATTTCTCATCGGTGCCTTGCTCTGAAAATTTGGGTCCTGATGACAACAGAACTGAAGAAGTGATTGGGTTGGTTTCATTGTCCTTGGTTAAATTATTAAAGACCACACTTGCACATGTTTCTTCTTATCATGTGATGCGTCTGGCATCACATCTGCAGCATAAGATGGATAATGGGTTGCATGTAATGACTCTTGTTTGGTTAGGAGAATATAACCAGTCTCAGACCAGAGGCCTCAATCAGCATTTGAATCAGGACATGCTCAAATTGGAGACAATAGGTGAAAAACATGGATCTGACATATTATGGGATATTTGTGCAGATCCTAAAATAATATCTGAGAGTTTCACCCAGGAAAAAATAAACTGGTCTCACTCCCTTGATCATAAACCGTCTAAAGGATGGAATAACATATATAGAGGCGTCACAATTGTACATGAGACTGAGGAAACTCATAACCATGAACTTACACCTAAGAGTACTTCTGCCAATAGTGAAGCTGGATTACCTTCTAGAAGTCTATTTCACAGTGGCCACTCTTTCCTTAGCGGATGGCAGAAAGATACAACCGCTACAAAGGAGGTCTCACCTTTCCTGAACCCAAAAGAAATATATAAGAGAAATGGGGAGCTTTTGGAGGCATTGTGTCTCAACTCTGTTAATCAGAAACAAGCTGCTCTTGCAAGCAACCGAAAGGGCATCATATTTTTTAATTGGAAAGATGATATGCCTGATAGAGATCATTCAGACTATATCTGGTCAGAGGCTGATTGGCCACTGAACGGGTGGGCTGGTTCTGAATCAACGCCAGCTCCAACGTATGTATCTCCTGGTGTTGGTCTTGGATTCAAACAAGGCACACACGTTGGACTGGGTGGAGCAACTGTTGGTGTAGGATCTTTGGCAAGGTCAGCAAGAGACTTGAGAGGTGGAGGAGCATTTGGGAATCAAGGTTATCCTGGAATGCGTGTGTCTGGTTTAGGTTGGGAGACTCGAGAGGACTTTGAGGAAGTTGTTGATCCACCACCTACTGTTGAGAATGCAAACACAAGGGTTTTTTCCAGTCACCCATCAAGACCTTTCTTCTTGGTTGGTTCTAGCAATACACACATTTACTTATGGGAGTTTGGCAAAGACAAAGCTACTGCAACTTATGGAGTTCTGCCTGCTGCAAATGTCCCTCCACCTTATGCTCTGGCATCGATATCTGCTTTGCAGTTTGATCACTGTGGACACAGATTTGCTACCGCTGCCTTAGATGGAACTGTATGCACATGGCAGCTGGAGGTGGGAGGAAGGAGTAATATTCGTCCTACAGAATCATCTCTATGCTTTAACAGTCATGCATCGGATGTTGCTTATGTTACATCAAGTGGATCAATCATTGCTGTGGCAGGATATAGCTCCAGTAGTGTTAATGTGGTCATATGGGATACATTGGCTCCACCTACTACCTCTCGAGCTTCCATTATTTGTCATGAAGGTGGGGCGCGCTCTCTTTCTGTTTTCGATAATGACATAGGAAGTGGATCTATCTCACCTCTTATTGTGACTGGTGGTAAAGGTGGTGATGTTGGACTTCACGACTTCCGGTATATTGCTACTGGAAGGAGTAAAAGGCACAGGCATACTGATAAAGGTGAACAAGCTGTGAAGACATCATCAAATATTGATCATCATCCTCCGGATGGTAACAGATTTGGGGAACAAAATCAGAATGGGATGCTGTGGTATATACCAAAAGCTCACTCAGGTATAACCTGCTGCAATTAA

Protein Analysis

2486

Amino Acids

275.17

Weight (kDa)

6.04

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rav1p_C PF12234 782 - 1442 1e-72 RAVE protein 1 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1299, 4316, 5646
AarI CACCTGC 1 cut(s) 2873
Acc36I ACCTGC 3 cut(s) 286, 2873, 7457
Acc65I GGTACC 1 cut(s) 3068
AccB1I GGYRCC 4 cut(s) 215, 1732, 3068, 5701
AccB7I CCANNNNNTGG 5 cut(s) 174, 613, 809, 960, 4400
AccBSI CCGCTC 1 cut(s) 4459
AccI GTMKAC 2 cut(s) 6, 1418
AccII CGCG 5 cut(s) 386, 3183, 3185, 5100, 7180
AccIII TCCGGA 4 cut(s) 59, 2030, 3657, 7372
AcoI YGGCCR 4 cut(s) 327, 381, 6220, 6461
AcuI CTGAAG 7 cut(s) 3896, 4206, 5156, 5298, 5468, 5504, 5763
AcyI GRCGYC 2 cut(s) 216, 6111
AdeI CACNNNGTG 1 cut(s) 960
AflIII ACRYGT 5 cut(s) 1270, 4825, 5098, 5803, 6550
AhlI ACTAGT 3 cut(s) 863, 1657, 2903
AjiI CACGTC 1 cut(s) 1038
AjuI GAANNNNNNNTTGG 8 cut(s) 3416, 3448, 3735, 3767, 4639, 4671, 5736, 5768
AloI GAACNNNNNNTCC 2 cut(s) 1239, 1271
Alw21I GWGCWC 2 cut(s) 309, 431
AlwNI CAGNNNCTG 4 cut(s) 1003, 4505, 4769, 6455
Ama87I CYCGRG 3 cut(s) 1221, 6681, 7146
Aor13HI TCCGGA 4 cut(s) 59, 2030, 3657, 7372
ApaI GGGCCC 1 cut(s) 1893
ArsI GACNNNNNNTTYG 2 cut(s) 2036, 2068
AseI ATTAAT 1 cut(s) 5381
Asp718I GGTACC 1 cut(s) 3068
AspLEI GCGC 5 cut(s) 218, 3183, 3185, 7180, 7182
AsuC2I CCSGG 4 cut(s) 159, 182, 1222, 1223
AsuNHI GCTAGC 2 cut(s) 704, 4420
AvaI CYCGRG 3 cut(s) 1221, 6681, 7146
AxyI CCTNAGG 1 cut(s) 3891
BaeGI GKGCMC 2 cut(s) 1735, 1893
BaeI ACNNNNGTAYC 6 cut(s) 4758, 4791, 5526, 5559, 6495, 6528
BalI TGGCCA 2 cut(s) 6222, 6463
BamHI GGATCC 3 cut(s) 161, 2485, 3886
BanI GGYRCC 4 cut(s) 215, 1732, 3068, 5701
BanII GRGCYC 4 cut(s) 309, 431, 509, 1893
BarI GAAGNNNNNNTAC 4 cut(s) 1746, 1778, 6177, 6209
BauI CACGAG 1 cut(s) 955
BbsI GAAGAC 5 cut(s) 1244, 2904, 3222, 5286, 7349
Bbv12I GWGCWC 2 cut(s) 309, 431
BceAI ACGGC 5 cut(s) 229, 314, 323, 346, 1632
BciVI GTATCC 2 cut(s) 3688, 7114
BclI TGATCA 7 cut(s) 943, 2245, 2542, 5397, 6070, 6910, 7360
BcnI CCSGG 4 cut(s) 159, 182, 1222, 1223
BcuI ACTAGT 3 cut(s) 863, 1657, 2903
BfmI CTRYAG 7 cut(s) 972, 2280, 2880, 4449, 5113, 5843, 7002
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 3 cut(s) 286, 2873, 7457
BfuI GTATCC 2 cut(s) 3688, 7114
BglII AGATCT 2 cut(s) 4582, 5002
BlpI GCTNAGC 1 cut(s) 1489
BmcAI AGTACT 2 cut(s) 4675, 6169
BmeT110I CYCGRG 3 cut(s) 1221, 6681, 7146
BmgBI CACGTC 1 cut(s) 1038
BmrI ACTGGG 5 cut(s) 19, 1062, 1202, 2606, 6569
BmtI GCTAGC 2 cut(s) 708, 4424
BmuI ACTGGG 5 cut(s) 19, 1062, 1202, 2606, 6569
BoxI GACNNNNGTC 1 cut(s) 3393
BpiI GAAGAC 5 cut(s) 1244, 2904, 3222, 5286, 7349
BpmI CTGGAG 8 cut(s) 89, 1800, 3503, 4094, 4301, 4316, 6992, 7078
Bpu10I CCTNAGC 3 cut(s) 508, 930, 6233
Bpu1102I GCTNAGC 1 cut(s) 1489
BpuEI CTTGAG 5 cut(s) 1354, 2657, 4977, 5514, 6631
BpuMI CCSGG 4 cut(s) 159, 182, 1222, 1223
Bsa29I ATCGAT 1 cut(s) 6890
BsaBI GATNNNNATC 4 cut(s) 3676, 5001, 6426, 7365
BsaHI GRCGYC 2 cut(s) 216, 6111
BsaI GGTCTC 6 cut(s) 211, 622, 3051, 4409, 6063, 6277
BsaWI WCCGGW 6 cut(s) 59, 2030, 3657, 3941, 7278, 7372
Bse118I RCCGGY 2 cut(s) 352, 4176
Bse21I CCTNAGG 1 cut(s) 3891
Bse3DI GCAATG 3 cut(s) 2703, 3902, 7071
Bse8I GATNNNNATC 4 cut(s) 3676, 5001, 6426, 7365
BseAI TCCGGA 4 cut(s) 59, 2030, 3657, 7372
BseCI ATCGAT 1 cut(s) 6890
BseJI GATNNNNATC 4 cut(s) 3676, 5001, 6426, 7365
BseMI GCAATG 3 cut(s) 2703, 3902, 7071
BsePI GCGCGC 2 cut(s) 3181, 7178
BseRI GAGGAG 6 cut(s) 211, 218, 313, 2329, 3895, 6636
BseSI GKGCMC 2 cut(s) 1735, 1893
BseX3I CGGCCG 2 cut(s) 327, 381
BsgI GTGCAG 2 cut(s) 2526, 6027
Bsh1236I CGCG 5 cut(s) 386, 3183, 3185, 5100, 7180
Bsh1285I CGRYCG 2 cut(s) 330, 384
BshNI GGYRCC 4 cut(s) 215, 1732, 3068, 5701
BshVI ATCGAT 1 cut(s) 6890
BsiEI CGRYCG 2 cut(s) 330, 384
BsiHKAI GWGCWC 2 cut(s) 309, 431
BsiHKCI CYCGRG 3 cut(s) 1221, 6681, 7146
BslFI GGGAC 7 cut(s) 26, 267, 1281, 1785, 2763, 4603, 6851
BsmBI CGTCTC 2 cut(s) 369, 2051
BsmFI GGGAC 7 cut(s) 26, 267, 1281, 1785, 2763, 4603, 6851
BsmI GAATGC 6 cut(s) 463, 1167, 4450, 4682, 6657, 6733
Bso31I GGTCTC 6 cut(s) 211, 622, 3051, 4409, 6063, 6277
BsoBI CYCGRG 3 cut(s) 1221, 6681, 7146
Bsp120I GGGCCC 1 cut(s) 1889
Bsp1286I GDGCHC 5 cut(s) 309, 431, 509, 1735, 1893
Bsp13I TCCGGA 4 cut(s) 59, 2030, 3657, 7372
Bsp1407I TGTACA 3 cut(s) 4938, 5329, 6121
Bsp1720I GCTNAGC 1 cut(s) 1489
Bsp19I CCATGG 2 cut(s) 78, 2351
BspDI ATCGAT 1 cut(s) 6890
BspEI TCCGGA 4 cut(s) 59, 2030, 3657, 7372
BspFNI CGCG 5 cut(s) 386, 3183, 3185, 5100, 7180
BspHI TCATGA 4 cut(s) 4494, 4819, 5289, 7165
BspMAI CTGCAG 3 cut(s) 2884, 5117, 5847
BspMI ACCTGC 3 cut(s) 286, 2873, 7457
BspOI GCTAGC 2 cut(s) 708, 4424
BspT107I GGYRCC 4 cut(s) 215, 1732, 3068, 5701
BspTNI GGTCTC 6 cut(s) 211, 622, 3051, 4409, 6063, 6277
BsrBI CCGCTC 1 cut(s) 4459
BsrDI GCAATG 3 cut(s) 2703, 3902, 7071
BsrFI RCCGGY 2 cut(s) 352, 4176
BsrGI TGTACA 3 cut(s) 4938, 5329, 6121
BssAI RCCGGY 2 cut(s) 352, 4176
BssHII GCGCGC 2 cut(s) 3181, 7178
BssNAI GTATAC 1 cut(s) 1419
BssNI GRCGYC 2 cut(s) 216, 6111
BssSI CACGAG 1 cut(s) 955
BssT1I CCWWGG 4 cut(s) 78, 2351, 4144, 5772
Bst1107I GTATAC 1 cut(s) 1419
Bst2BI CACGAG 1 cut(s) 955
Bst6I CTCTTC 7 cut(s) 780, 2478, 2962, 3226, 3872, 4008, 5378
BstACI GRCGYC 2 cut(s) 216, 6111
BstAPI GCANNNNNTGC 3 cut(s) 656, 2403, 3790
BstAUI TGTACA 3 cut(s) 4938, 5329, 6121
BstDSI CCRYGG 4 cut(s) 78, 384, 2351, 3681
BstEII GGTNACC 1 cut(s) 5479
BstENI CCTNNNNNAGG 1 cut(s) 1863
BstFNI CGCG 5 cut(s) 386, 3183, 3185, 5100, 7180
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 5 cut(s) 218, 3183, 3185, 7180, 7182
BstMCI CGRYCG 2 cut(s) 330, 384
BstPAI GACNNNNGTC 1 cut(s) 3393
BstPI GGTNACC 1 cut(s) 5479
BstSFI CTRYAG 7 cut(s) 972, 2280, 2880, 4449, 5113, 5843, 7002
BstSLI GKGCMC 2 cut(s) 1735, 1893
BstUI CGCG 5 cut(s) 386, 3183, 3185, 5100, 7180
BstV2I GAAGAC 5 cut(s) 1244, 2904, 3222, 5286, 7349
BstXI CCANNNNNNTGG 1 cut(s) 734
BstZ17I GTATAC 1 cut(s) 1419
BstZI CGGCCG 2 cut(s) 327, 381
Bsu15I ATCGAT 1 cut(s) 6890
Bsu36I CCTNAGG 1 cut(s) 3891
BsuI GTATCC 2 cut(s) 3688, 7114
BsuTUI ATCGAT 1 cut(s) 6890
BtgI CCRYGG 4 cut(s) 78, 384, 2351, 3681
BtgZI GCGATG 1 cut(s) 2127
BtrI CACGTC 1 cut(s) 1038
BtsI GCAGTG 3 cut(s) 468, 1551, 4503
BveI ACCTGC 3 cut(s) 286, 2873, 7457
CaiI CAGNNNCTG 4 cut(s) 1003, 4505, 4769, 6455
CciI TCATGA 4 cut(s) 4494, 4819, 5289, 7165
CciNI GCGGCCGC 1 cut(s) 381
CfoI GCGC 5 cut(s) 218, 3183, 3185, 7180, 7182
Cfr10I RCCGGY 2 cut(s) 352, 4176
Cfr42I CCGCGG 1 cut(s) 387
Cfr9I CCCGGG 1 cut(s) 1221
ClaI ATCGAT 1 cut(s) 6890
CseI GACGC 2 cut(s) 5816, 6100
CsiI ACCWGGT 1 cut(s) 1462
CspCI CAANNNNNGTGG 6 cut(s) 3267, 3302, 3716, 3751, 4659, 4694
DinI GGCGCC 1 cut(s) 217
DraI TTTAAA 1 cut(s) 592
DraIII CACNNNGTG 1 cut(s) 960
EaeI YGGCCR 4 cut(s) 327, 381, 6220, 6461
EagI CGGCCG 2 cut(s) 327, 381
Eam1104I CTCTTC 7 cut(s) 780, 2478, 2962, 3226, 3872, 4008, 5378
EarI CTCTTC 7 cut(s) 780, 2478, 2962, 3226, 3872, 4008, 5378
EciI GGCGGA 3 cut(s) 124, 154, 4838
Ecl136II GAGCTC 2 cut(s) 307, 429
EclXI CGGCCG 2 cut(s) 327, 381
Eco130I CCWWGG 4 cut(s) 78, 2351, 4144, 5772
Eco147I AGGCCT 2 cut(s) 5517, 5922
Eco24I GRGCYC 4 cut(s) 309, 431, 509, 1893
Eco31I GGTCTC 6 cut(s) 211, 622, 3051, 4409, 6063, 6277
Eco32I GATATC 2 cut(s) 1675, 6894
Eco52I CGGCCG 2 cut(s) 327, 381
Eco53kI GAGCTC 2 cut(s) 307, 429
Eco57I CTGAAG 7 cut(s) 3896, 4206, 5156, 5298, 5468, 5504, 5763
Eco81I CCTNAGG 1 cut(s) 3891
Eco88I CYCGRG 3 cut(s) 1221, 6681, 7146
Eco91I GGTNACC 1 cut(s) 5479
EcoICRI GAGCTC 2 cut(s) 307, 429
EcoNI CCTNNNNNAGG 1 cut(s) 1863
EcoO109I RGGNCCY 6 cut(s) 641, 2234, 2387, 2750, 3145, 5723
EcoO65I GGTNACC 1 cut(s) 5479
EcoRI GAATTC 2 cut(s) 3927, 4629
EcoRV GATATC 2 cut(s) 1675, 6894
EcoT14I CCWWGG 4 cut(s) 78, 2351, 4144, 5772
EcoT22I ATGCAT 2 cut(s) 5052, 7036
EcoT38I GRGCYC 4 cut(s) 309, 431, 509, 1893
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
ErhI CCWWGG 4 cut(s) 78, 2351, 4144, 5772
Esp3I CGTCTC 2 cut(s) 369, 2051
FaqI GGGAC 7 cut(s) 26, 267, 1281, 1785, 2763, 4603, 6851
FauI CCCGC 1 cut(s) 162
FauNDI CATATG 3 cut(s) 3406, 4048, 7115
FbaI TGATCA 7 cut(s) 943, 2245, 2542, 5397, 6070, 6910, 7360
FblI GTMKAC 2 cut(s) 6, 1418
FriOI GRGCYC 4 cut(s) 309, 431, 509, 1893
GlaI GCGC 5 cut(s) 217, 3182, 3184, 7179, 7181
GsuI CTGGAG 8 cut(s) 89, 1800, 3503, 4094, 4301, 4316, 6992, 7078
HaeII RGCGCY 1 cut(s) 219
HgaI GACGC 2 cut(s) 5816, 6100
HhaI GCGC 5 cut(s) 218, 3183, 3185, 7180, 7182
Hin1I GRCGYC 2 cut(s) 216, 6111
Hin6I GCGC 5 cut(s) 216, 3181, 3183, 7178, 7180
HinP1I GCGC 5 cut(s) 216, 3181, 3183, 7178, 7180
HincII GTYRAC 3 cut(s) 693, 3294, 3466
HindII GTYRAC 3 cut(s) 693, 3294, 3466
HindIII AAGCTT 2 cut(s) 1685, 4536
HpyCH4IV ACGT 3 cut(s) 1037, 6506, 6552
HpySE526I ACGT 3 cut(s) 1037, 6506, 6552
Hsp92I GRCGYC 2 cut(s) 216, 6111
HspAI GCGC 5 cut(s) 216, 3181, 3183, 7178, 7180
KasI GGCGCC 1 cut(s) 215
KflI GGGWCCC 1 cut(s) 2750
Kpn2I TCCGGA 4 cut(s) 59, 2030, 3657, 7372
KpnI GGTACC 1 cut(s) 3072
KroI GCCGGC 1 cut(s) 4176
KroNI GCCGGC 1 cut(s) 4178
Ksp22I TGATCA 7 cut(s) 943, 2245, 2542, 5397, 6070, 6910, 7360
KspI CCGCGG 1 cut(s) 387
MabI ACCWGGT 1 cut(s) 1462
MaeII ACGT 3 cut(s) 1037, 6506, 6552
MbiI CCGCTC 1 cut(s) 4459
MfeI CAATTG 5 cut(s) 492, 3050, 3833, 4245, 6117
MhlI GDGCHC 5 cut(s) 309, 431, 509, 1735, 1893
MlsI TGGCCA 2 cut(s) 6222, 6463
MluI ACGCGT 1 cut(s) 5098
MluNI TGGCCA 2 cut(s) 6222, 6463
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 9 cut(s) 57, 3236, 3383, 3404, 3695, 4253, 4323, 5872, 6673
MmeI TCCRAC 9 cut(s) 65, 174, 428, 1656, 1676, 1946, 6527, 6535, 7243
Mox20I TGGCCA 2 cut(s) 6222, 6463
Mph1103I ATGCAT 2 cut(s) 5052, 7036
MroI TCCGGA 4 cut(s) 59, 2030, 3657, 7372
MroNI GCCGGC 1 cut(s) 4176
MscI TGGCCA 2 cut(s) 6222, 6463
MslI CAYNNNNRTG 9 cut(s) 1815, 3207, 4803, 4824, 4919, 6147, 7015, 7076, 7170
Msp20I TGGCCA 2 cut(s) 6222, 6463
MspA1I CMGCKG 7 cut(s) 386, 980, 1493, 2765, 4031, 6938, 6970
MunI CAATTG 5 cut(s) 492, 3050, 3833, 4245, 6117
Mva1269I GAATGC 6 cut(s) 463, 1167, 4450, 4682, 6657, 6733
MvnI CGCG 5 cut(s) 386, 3183, 3185, 5100, 7180
NaeI GCCGGC 1 cut(s) 4178
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 4 cut(s) 159, 182, 1222, 1223
NcoI CCATGG 2 cut(s) 78, 2351
NdeI CATATG 3 cut(s) 3406, 4048, 7115
NgoMIV GCCGGC 1 cut(s) 4176
NheI GCTAGC 2 cut(s) 704, 4420
NmeAIII GCCGAG 3 cut(s) 232, 305, 348
NotI GCGGCCGC 1 cut(s) 381
NsiI ATGCAT 2 cut(s) 5052, 7036
PaeI GCATGC 2 cut(s) 2611, 4324
PaeR7I CTCGAG 2 cut(s) 6681, 7146
PagI TCATGA 4 cut(s) 4494, 4819, 5289, 7165
PaqCI CACCTGC 1 cut(s) 2873
PauI GCGCGC 2 cut(s) 3181, 7178
PceI AGGCCT 2 cut(s) 5517, 5922
PciI ACATGT 3 cut(s) 1270, 4825, 5803
PctI GAATGC 6 cut(s) 463, 1167, 4450, 4682, 6657, 6733
PdiI GCCGGC 1 cut(s) 4178
PflMI CCANNNNNTGG 5 cut(s) 174, 613, 809, 960, 4400
PfoI TCCNGGA 4 cut(s) 180, 1156, 1257, 6646
PleI GAGTC 9 cut(s) 57, 3235, 3383, 3404, 3695, 4253, 4323, 5872, 6673
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 9 cut(s) 57, 3235, 3383, 3404, 3695, 4253, 4323, 5872, 6673
PpuMI RGGWCCY 4 cut(s) 641, 2234, 2750, 5723
PscI ACATGT 3 cut(s) 1270, 4825, 5803
PshAI GACNNNNGTC 1 cut(s) 3393
PshBI ATTAAT 1 cut(s) 5381
PsiI TTATAA 3 cut(s) 1299, 4316, 5646
Psp124BI GAGCTC 2 cut(s) 309, 431
Psp5II RGGWCCY 4 cut(s) 641, 2234, 2750, 5723
PspEI GGTNACC 1 cut(s) 5479
PspOMI GGGCCC 1 cut(s) 1889
PspPPI RGGWCCY 4 cut(s) 641, 2234, 2750, 5723
PstI CTGCAG 3 cut(s) 2884, 5117, 5847
PstNI CAGNNNCTG 4 cut(s) 1003, 4505, 4769, 6455
PteI GCGCGC 2 cut(s) 3181, 7178
PvuII CAGCTG 4 cut(s) 1493, 2765, 4031, 6970
RseI CAYNNNNRTG 9 cut(s) 1815, 3207, 4803, 4824, 4919, 6147, 7015, 7076, 7170
SacI GAGCTC 2 cut(s) 309, 431
SacII CCGCGG 1 cut(s) 387
ScaI AGTACT 2 cut(s) 4675, 6169
SchI GAGTC 9 cut(s) 57, 3236, 3383, 3404, 3695, 4253, 4323, 5872, 6673
SduI GDGCHC 5 cut(s) 309, 431, 509, 1735, 1893
SexAI ACCWGGT 1 cut(s) 1462
SfcI CTRYAG 7 cut(s) 972, 2280, 2880, 4449, 5113, 5843, 7002
SfoI GGCGCC 1 cut(s) 217
Sfr274I CTCGAG 2 cut(s) 6681, 7146
Sfr303I CCGCGG 1 cut(s) 387
SgrBI CCGCGG 1 cut(s) 387
SlaI CTCGAG 2 cut(s) 6681, 7146
SmaI CCCGGG 1 cut(s) 1223
SmiMI CAYNNNNRTG 9 cut(s) 1815, 3207, 4803, 4824, 4919, 6147, 7015, 7076, 7170
SmlI CTYRAG 7 cut(s) 1333, 2636, 4992, 5529, 6610, 6681, 7146
SmoI CTYRAG 7 cut(s) 1333, 2636, 4992, 5529, 6610, 6681, 7146
SpeI ACTAGT 3 cut(s) 863, 1657, 2903
SphI GCATGC 2 cut(s) 2611, 4324
SseBI AGGCCT 2 cut(s) 5517, 5922
SspDI GGCGCC 1 cut(s) 215
SspI AATATT 3 cut(s) 4974, 6994, 7357
SstI GAGCTC 2 cut(s) 309, 431
StuI AGGCCT 2 cut(s) 5517, 5922
StyI CCWWGG 4 cut(s) 78, 2351, 4144, 5772
TaiI ACGT 3 cut(s) 1040, 6509, 6555
TaqII GACCGA 2 cut(s) 269, 1142
TatI WGTACW 5 cut(s) 4673, 4938, 5329, 6121, 6167
TauI GCSGC 4 cut(s) 383, 386, 389, 980
TspGWI ACGGA 3 cut(s) 924, 3670, 4676
TspMI CCCGGG 1 cut(s) 1221
Van91I CCANNNNNTGG 5 cut(s) 174, 613, 809, 960, 4400
VspI ATTAAT 1 cut(s) 5381
XagI CCTNNNNNAGG 1 cut(s) 1863
XbaI TCTAGA 1 cut(s) 6200
XcmI CCANNNNNNNNNTGG 4 cut(s) 1004, 1639, 4711, 6471
XhoI CTCGAG 2 cut(s) 6681, 7146
XmaI CCCGGG 1 cut(s) 1221
XmiI GTMKAC 2 cut(s) 6, 1418
ZrmI AGTACT 2 cut(s) 4675, 6169
Zsp2I ATGCAT 2 cut(s) 5052, 7036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.