Rroxscaffold_6G00388270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009732
Physical Location & Seq
Forward (+)
1148674 .. 1174351
25678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00388270.1

Sequence Viewer

Length: 795 bp
ATGGTGCTCATGGAGACTCCTCCACAAGGGGTCATTTCCAAGCAAGAAGTATTGAATACTATATTTAAACTGTTCAAAGAGTTTTGGCCAAGTGGGTATGCTTTAATTTTGTTTGCCATTGTGGAACTGCTTAGGGGCTCAAGCTTAATTAACTATGCTTCAATGTTGGTTTCTACGGTGAGAAGGATGCTCGGATGTGTATATACGATGCTTCTTGGGATACTCATTTTGGCTTTTGCTGTGATGTTAATGATGGAACTTCCTAGAAGCTTGCAACGTCCATTACACAAATTCAATCAAACAAGACTTCCTCAAGCAATGTTTGATGCCATTCTAACGCCCTACGGCGAATCCCAAACCACACTCATTTCCTCCCCACTAGGAGCCTTTTTGTCATCGCATGACATCAAGCACGTACCCGAAATTCAAGGAGTGCAATGCCTAAGGTGGAAGGGTACTGCTAGAGGAGGAGTCCAAGCTGTGCCTCTACAGCTGCTCCCATTCTCACTCGTGCTCGTGCTTGAGGCAGAACATGCATTGCATCAGAACCAATTAATTGAGGTTGGTTTGCCTATTCATTTTTCACTTACTCATACCTCATCTACATTTTTACATGTATCCGACTTAGGCATCAAGGAGTCGAAGGCCGGCAACCCCGTTTTTCCCTCTAACCTGTATTCATGGTTGATAGGTATGGAACTCGAAGATGGTTGCTCTCACGGTGATTCTCACAGAAATAGTAACAAGCACCACAGAGGGATCAAAATGTTGCGACCATGGGAGGACGCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

29.51

Weight (kDa)

7.84

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 767
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 2 cut(s) 290, 423
AfaI GTAC 2 cut(s) 417, 457
AfiI CCNNNNNNNGG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 613
AgsI TTSAA 5 cut(s) 55, 76, 162, 295, 428
AluBI AGCT 4 cut(s) 144, 270, 479, 493
AluI AGCT 4 cut(s) 144, 270, 479, 493
Alw21I GWGCWC 2 cut(s) 9, 516
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 1 cut(s) 767
AoxI GGCC 2 cut(s) 86, 645
ApeKI GCWGC 1 cut(s) 493
ApoI RAATTY 2 cut(s) 290, 423
AseI ATTAAT 1 cut(s) 554
AsuHPI GGTGA 2 cut(s) 190, 734
AxyI CCTNAGG 1 cut(s) 443
BalI TGGCCA 1 cut(s) 88
BanII GRGCYC 1 cut(s) 140
BauI CACGAG 2 cut(s) 509, 515
Bbv12I GWGCWC 2 cut(s) 9, 516
BbvI GCAGC 1 cut(s) 480
BccI CCATC 2 cut(s) 247, 701
BceAI ACGGC 1 cut(s) 361
BciVI GTATCC 2 cut(s) 213, 628
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 3 cut(s) 264, 380, 462
BfmI CTRYAG 1 cut(s) 488
BfuI GTATCC 2 cut(s) 213, 628
BisI GCNGC 1 cut(s) 494
BlsI GCNGC 1 cut(s) 495
BmiI GGNNCC 1 cut(s) 385
BmsI GCATC 5 cut(s) 177, 198, 316, 550, 639
Bpu10I CCTNAGC 1 cut(s) 131
BpuEI CTTGAG 3 cut(s) 124, 297, 542
BsaAI YACGTR 1 cut(s) 415
BsaJI CCNNGG 1 cut(s) 776
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 647
Bse21I CCTNAGG 1 cut(s) 443
Bse3DI GCAATG 3 cut(s) 324, 443, 536
BseDI CCNNGG 1 cut(s) 776
BseGI GGATG 2 cut(s) 192, 200
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 3 cut(s) 324, 443, 536
BseRI GAGGAG 3 cut(s) 9, 480, 483
BseXI GCAGC 1 cut(s) 480
BshFI GGCC 2 cut(s) 88, 647
BsiHKAI GWGCWC 2 cut(s) 9, 516
BsiSI CCGG 1 cut(s) 648
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 8
BsnI GGCC 2 cut(s) 88, 647
Bsp1286I GDGCHC 3 cut(s) 9, 140, 516
Bsp143I GATC 1 cut(s) 759
Bsp19I CCATGG 1 cut(s) 776
BspANI GGCC 2 cut(s) 88, 647
BspLI GGNNCC 1 cut(s) 385
BspPI GGATC 1 cut(s) 767
BsrDI GCAATG 3 cut(s) 324, 443, 536
BsrFI RCCGGY 1 cut(s) 647
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 1 cut(s) 776
BssMI GATC 1 cut(s) 759
BssSI CACGAG 2 cut(s) 509, 515
BssT1I CCWWGG 1 cut(s) 776
Bst2BI CACGAG 2 cut(s) 509, 515
Bst4CI ACNGT 3 cut(s) 72, 178, 722
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 272, 649
BstDEI CTNAG 3 cut(s) 131, 443, 625
BstDSI CCRYGG 1 cut(s) 776
BstENI CCTNNNNNAGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 192, 200
BstKTI GATC 1 cut(s) 762
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 1 cut(s) 759
BstMWI GCNNNNNNNGC 2 cut(s) 490, 533
BstNSI RCATGY 2 cut(s) 536, 617
BstSFI CTRYAG 1 cut(s) 488
BstV1I GCAGC 1 cut(s) 480
Bsu36I CCTNAGG 1 cut(s) 443
BsuI GTATCC 2 cut(s) 213, 628
BsuRI GGCC 2 cut(s) 88, 647
BtgI CCRYGG 1 cut(s) 776
BtgZI GCGATG 1 cut(s) 381
BtsCI GGATG 2 cut(s) 192, 200
Cac8I GCNNGC 2 cut(s) 272, 649
Cfr10I RCCGGY 1 cut(s) 647
Csp6I GTAC 2 cut(s) 416, 456
CviAII CATG 6 cut(s) 10, 401, 533, 614, 681, 777
CviJI RGCY 9 cut(s) 88, 138, 144, 233, 270, 386, 479, 493, 647
CviKI_1 RGCY 9 cut(s) 88, 138, 144, 233, 270, 386, 479, 493, 647
CviQI GTAC 2 cut(s) 416, 456
DdeI CTNAG 3 cut(s) 131, 443, 625
DpnI GATC 1 cut(s) 761
DpnII GATC 1 cut(s) 759
DraI TTTAAA 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 776
Eco24I GRGCYC 1 cut(s) 140
Eco81I CCTNAGG 1 cut(s) 443
EcoNI CCTNNNNNAGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 776
EcoT22I ATGCAT 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 776
FaeI CATG 6 cut(s) 13, 404, 536, 617, 684, 780
FatI CATG 6 cut(s) 9, 400, 532, 613, 680, 776
Fnu4HI GCNGC 1 cut(s) 494
FokI GGATG 2 cut(s) 199, 207
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 494
FspBI CTAG 3 cut(s) 264, 380, 462
GluI GCNGC 1 cut(s) 494
HaeIII GGCC 2 cut(s) 88, 647
HapII CCGG 1 cut(s) 648
Hin1II CATG 6 cut(s) 13, 404, 536, 617, 684, 780
HindIII AAGCTT 2 cut(s) 142, 268
HinfI GANTC 5 cut(s) 16, 350, 471, 638, 725
HpaII CCGG 1 cut(s) 648
HphI GGTGA 2 cut(s) 190, 734
Hpy188I TCNGA 3 cut(s) 194, 546, 622
HpyAV CCTTC 3 cut(s) 177, 445, 637
HpyCH4III ACNGT 3 cut(s) 72, 178, 722
HpyCH4IV ACGT 2 cut(s) 277, 414
HpyCH4V TGCA 4 cut(s) 274, 436, 536, 541
HpyF10VI GCNNNNNNNGC 2 cut(s) 490, 533
HpyF3I CTNAG 3 cut(s) 131, 443, 625
HpySE526I ACGT 2 cut(s) 277, 414
Hsp92II CATG 6 cut(s) 13, 404, 536, 617, 684, 780
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
Kzo9I GATC 1 cut(s) 759
LmnI GCTCC 2 cut(s) 383, 501
LpnPI CCDG 2 cut(s) 661, 686
Lsp1109I GCAGC 1 cut(s) 480
LweI GCATC 5 cut(s) 177, 198, 316, 550, 639
MaeI CTAG 3 cut(s) 264, 380, 462
MaeII ACGT 2 cut(s) 277, 414
MaeIII GTNAC 1 cut(s) 740
MalI GATC 1 cut(s) 761
MboI GATC 1 cut(s) 759
MboII GAAGA 1 cut(s) 716
MhlI GDGCHC 3 cut(s) 9, 140, 516
MlsI TGGCCA 1 cut(s) 88
MluCI AATT 6 cut(s) 105, 147, 290, 423, 551, 555
MluNI TGGCCA 1 cut(s) 88
MlyI GAGTC 3 cut(s) 10, 480, 647
MmeI TCCRAC 1 cut(s) 645
Mox20I TGGCCA 1 cut(s) 88
Mph1103I ATGCAT 1 cut(s) 538
MroNI GCCGGC 1 cut(s) 647
MscI TGGCCA 1 cut(s) 88
MseI TTAA 6 cut(s) 66, 104, 146, 150, 248, 554
Msp20I TGGCCA 1 cut(s) 88
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 1 cut(s) 648
MwoI GCNNNNNNNGC 2 cut(s) 490, 533
NaeI GCCGGC 1 cut(s) 649
NcoI CCATGG 1 cut(s) 776
NdeII GATC 1 cut(s) 759
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 6 cut(s) 13, 404, 536, 617, 684, 780
NlaIV GGNNCC 1 cut(s) 385
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 2 cut(s) 536, 617
PacI TTAATTAA 1 cut(s) 150
PciI ACATGT 1 cut(s) 613
PdiI GCCGGC 1 cut(s) 649
PfeI GAWTC 2 cut(s) 350, 725
PflFI GACNNNGTC 1 cut(s) 788
PkrI GCNGC 1 cut(s) 495
PleI GAGTC 3 cut(s) 10, 479, 646
PpsI GAGTC 3 cut(s) 10, 479, 646
Ppu21I YACGTR 1 cut(s) 415
PscI ACATGT 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 554
PspN4I GGNNCC 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 2 cut(s) 417, 457
RsaNI GTAC 2 cut(s) 416, 456
SaqAI TTAA 6 cut(s) 66, 104, 146, 150, 248, 554
SatI GCNGC 1 cut(s) 494
Sau3AI GATC 1 cut(s) 759
SchI GAGTC 3 cut(s) 10, 480, 647
SduI GDGCHC 3 cut(s) 9, 140, 516
SfaNI GCATC 5 cut(s) 177, 198, 316, 550, 639
SfcI CTRYAG 1 cut(s) 488
SmlI CTYRAG 3 cut(s) 139, 312, 521
SmoI CTYRAG 3 cut(s) 139, 312, 521
Sse9I AATT 6 cut(s) 105, 147, 290, 423, 551, 555
SspMI CTAG 3 cut(s) 264, 380, 462
StyI CCWWGG 1 cut(s) 776
TaaI ACNGT 3 cut(s) 72, 178, 722
TaiI ACGT 2 cut(s) 280, 417
TaqI TCGA 2 cut(s) 641, 702
TasI AATT 6 cut(s) 105, 147, 290, 423, 551, 555
TfiI GAWTC 2 cut(s) 350, 725
Tru1I TTAA 6 cut(s) 66, 104, 146, 150, 248, 554
Tru9I TTAA 6 cut(s) 66, 104, 146, 150, 248, 554
TseI GCWGC 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 566, 669
Tth111I GACNNNGTC 1 cut(s) 788
VspI ATTAAT 1 cut(s) 554
XagI CCTNNNNNAGG 1 cut(s) 24
XapI RAATTY 2 cut(s) 290, 423
XceI RCATGY 2 cut(s) 536, 617
XspI CTAG 3 cut(s) 264, 380, 462
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.