Rroxscaffold_6G00409300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010700
Physical Location & Seq
Forward (+)
31827173 .. 31830656
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409300.1

Sequence Viewer

Length: 849 bp
ATGGCGAACAAGATGAAGTTTTGGGGTGTTGAGGTAAAGAGTGGAGAGTCTATTGTTGTTGAACCTGGTATTACCCTTGTCCATCTTTCACAGGCCTGTCTGGATGAGGTCAAGAAAGGAAAGGGAAGTGATTCTATTTCACTCTTTGTGAAAACCGGTGATCAGAAGCTTGTTATTGGACATCTTTATGCTGATAAACTACCTCAGATATATTTTGATATTGTGTTTAATAAAAAATTTGTGCTATCTCATAATGGGAAAAGTGGGAGCGTCCACTTCCATGGATATATTGCTAATACGGGATATCCTTTTTCTGAACCAAAGGTGAGTCTTGAGGAAGCTAAGAAAGAAATTAAATCTGATCAGAAGGATTCTAGTAGTGATGATGATGATGATGAAGAATCTGAAGATGAAGAGTTTGATCAGGTCAAGAGTGTGGAAGATGGCAGTGAAGATGATGAAGAAAGTAGTGATGATGATGATAGTGAAGATGAAGATGATGACAGCGAGGAAGAGACTCCAAATAAGTCTGATTCCTTGACAATGAAATTGTTCAAGGTTGATGGGAGCAACAAACGTGCTGCTGAGAGTAGTAAAACACCTGTTCAAAAGAAGGCAAAGTTTGTTACTCCTGAAAAAACTAGTGGCAAGAAAGGAGCTGTCCACACAGCAACTCCTTACCCTTCAAAACAGGCTCTCAAAAGACCAGCTAATACTGACCAGACAAAGCTGCAGAGCTCAAAATCAAGTGGTGGTGGTACATTCCGTTGTGATCCCTGCAATAGGTCGTTTAACTCAGATGGTGCACTGCAGAGTCATACAAAGGCAAAGCATGGAGCTGCAAAGTGA

Protein Analysis

282

Amino Acids

30.86

Weight (kDa)

5.1

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPL PF17800 7 - 97 9.8e-15 Nucleoplasmin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010700)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03740
fragaria_vesca FvH4_6g17790
malus_domestica MD12G1017000.v1.1 MD14G1014900.v1.1
prunus_persica Prupe.7G118500_v2.0.a1 Prupe.7G118500_v2.0.a1
pyrus_communis pycom12g01380 pycom14g01240
rosa_chinensis RchiOBHm_Chr3g0472041
rosa_laevigata RLG00000024110
rosa_multiflora Rmu_sc0002532.1_g000010
rosa_roxburghii Rroxscaffold_6G00409300
rosa_rugosa Rorug03G0121800
rosa_samantha Rh3AG173400 Rh3AG173500 Rh3BG198900 Rh3CG190000 Rh3CG197400 Rh3DG175600
rosa_wichuraiana Rw3G016710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 767
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 1 cut(s) 760
AfiI CCNNNNNNNGG 1 cut(s) 783
AgeI ACCGGT 1 cut(s) 155
AgsI TTSAA 4 cut(s) 62, 556, 608, 687
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 7 cut(s) 169, 341, 659, 710, 730, 738, 839
AluI AGCT 7 cut(s) 169, 341, 659, 710, 730, 738, 839
Alw21I GWGCWC 2 cut(s) 740, 808
Alw26I GTCTC 1 cut(s) 509
Alw44I GTGCAC 1 cut(s) 804
AlwI GGATC 1 cut(s) 767
AoxI GGCC 1 cut(s) 93
ApaLI GTGCAC 1 cut(s) 804
ApeKI GCWGC 3 cut(s) 581, 730, 839
ApoI RAATTY 1 cut(s) 236
AsiGI ACCGGT 1 cut(s) 155
Asp700I GAANNNNTTC 2 cut(s) 130, 551
AsuHPI GGTGA 2 cut(s) 170, 337
BaeGI GKGCMC 1 cut(s) 808
BanII GRGCYC 1 cut(s) 740
Bbv12I GWGCWC 2 cut(s) 740, 808
BbvI GCAGC 3 cut(s) 568, 717, 826
BccI CCATC 4 cut(s) 90, 437, 557, 794
BciT130I CCWGG 1 cut(s) 66
BclI TGATCA 3 cut(s) 160, 361, 421
BcoDI GTCTC 1 cut(s) 509
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 2 cut(s) 375, 642
BfmI CTRYAG 2 cut(s) 731, 809
BisI GCNGC 3 cut(s) 582, 731, 840
BlsI GCNGC 3 cut(s) 583, 732, 841
Bme1390I CCNGG 1 cut(s) 66
BmrFI CCNGG 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 353
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 1 cut(s) 783
Bse118I RCCGGY 1 cut(s) 155
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 1 cut(s) 783
BseMII CTCAG 3 cut(s) 218, 576, 810
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 3 cut(s) 568, 717, 826
BshFI GGCC 1 cut(s) 95
BshTI ACCGGT 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 740, 808
BsiSI CCGG 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 783
BsmAI GTCTC 1 cut(s) 509
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 740, 808
Bsp143I GATC 4 cut(s) 160, 361, 421, 772
Bsp19I CCATGG 1 cut(s) 280
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 3 cut(s) 217, 577, 809
BspMAI CTGCAG 2 cut(s) 735, 813
BspPI GGATC 1 cut(s) 767
BsrFI RCCGGY 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 4 cut(s) 160, 361, 421, 772
BssT1I CCWWGG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 66
Bst6I CTCTTC 2 cut(s) 408, 507
BstDEI CTNAG 4 cut(s) 204, 342, 585, 796
BstDSI CCRYGG 1 cut(s) 280
BstENI CCTNNNNNAGG 1 cut(s) 781
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 4 cut(s) 163, 364, 424, 775
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 4 cut(s) 160, 361, 421, 772
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 2 cut(s) 731, 809
BstSLI GKGCMC 1 cut(s) 808
BstV1I GCAGC 3 cut(s) 568, 717, 826
BstXI CCANNNNNNTGG 1 cut(s) 281
BsuRI GGCC 1 cut(s) 95
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 2 cut(s) 454, 806
BtsIMutI CAGTG 2 cut(s) 454, 806
Cfr10I RCCGGY 1 cut(s) 155
CseI GACGC 1 cut(s) 259
CsiI ACCWGGT 1 cut(s) 64
Csp6I GTAC 1 cut(s) 759
CspAI ACCGGT 1 cut(s) 155
CviAII CATG 2 cut(s) 281, 833
CviJI RGCY 9 cut(s) 95, 169, 341, 659, 695, 710, 730, 738, 839
CviKI_1 RGCY 9 cut(s) 95, 169, 341, 659, 695, 710, 730, 738, 839
CviQI GTAC 1 cut(s) 759
DdeI CTNAG 4 cut(s) 204, 342, 585, 796
DpnI GATC 4 cut(s) 162, 363, 423, 774
DpnII GATC 4 cut(s) 160, 361, 421, 772
Eam1104I CTCTTC 2 cut(s) 408, 507
EarI CTCTTC 2 cut(s) 408, 507
Ecl136II GAGCTC 1 cut(s) 738
Eco130I CCWWGG 1 cut(s) 280
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 740
Eco32I GATATC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 738
Eco57I CTGAAG 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 738
EcoNI CCTNNNNNAGG 1 cut(s) 781
EcoRII CCWGG 1 cut(s) 64
EcoRV GATATC 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 2 cut(s) 284, 836
FaiI YATR 7 cut(s) 189, 211, 252, 282, 288, 819, 834
FatI CATG 2 cut(s) 280, 832
FbaI TGATCA 3 cut(s) 160, 361, 421
Fnu4HI GCNGC 3 cut(s) 582, 731, 840
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 3 cut(s) 582, 731, 840
FspBI CTAG 2 cut(s) 375, 642
GluI GCNGC 3 cut(s) 582, 731, 840
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 259
Hin1II CATG 2 cut(s) 284, 836
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 8 cut(s) 47, 131, 328, 371, 401, 517, 533, 814
HpaII CCGG 1 cut(s) 156
HphI GGTGA 2 cut(s) 170, 337
Hpy166II GTNNAC 3 cut(s) 274, 664, 806
Hpy188I TCNGA 8 cut(s) 165, 207, 316, 361, 366, 406, 532, 799
Hpy188III TCNNGA 5 cut(s) 101, 112, 332, 430, 632
Hpy8I GTNNAC 3 cut(s) 274, 664, 806
HpyAV CCTTC 3 cut(s) 361, 607, 693
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 5 cut(s) 733, 780, 806, 811, 842
HpyF3I CTNAG 4 cut(s) 204, 342, 585, 796
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 2 cut(s) 284, 836
Ksp22I TGATCA 3 cut(s) 160, 361, 421
Kzo9I GATC 4 cut(s) 160, 361, 421, 772
LmnI GCTCC 4 cut(s) 267, 567, 656, 836
Lsp1109I GCAGC 3 cut(s) 568, 717, 826
MabI ACCWGGT 1 cut(s) 64
MaeI CTAG 2 cut(s) 375, 642
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 1 cut(s) 625
MalI GATC 4 cut(s) 162, 363, 423, 774
MboI GATC 4 cut(s) 160, 361, 421, 772
MboII GAAGA 9 cut(s) 410, 419, 425, 452, 464, 473, 500, 506, 524
MhlI GDGCHC 2 cut(s) 740, 808
MluCI AATT 3 cut(s) 236, 351, 548
MlyI GAGTC 4 cut(s) 56, 337, 511, 823
MnlI CCTC 5 cut(s) 25, 100, 213, 328, 502
MroXI GAANNNNTTC 2 cut(s) 130, 551
MseI TTAA 3 cut(s) 228, 354, 792
MslI CAYNNNNRTG 2 cut(s) 186, 279
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 280
NdeII GATC 4 cut(s) 160, 361, 421, 772
NlaIII CATG 2 cut(s) 284, 836
PceI AGGCCT 1 cut(s) 95
PdmI GAANNNNTTC 2 cut(s) 130, 551
PfeI GAWTC 4 cut(s) 131, 371, 401, 533
PinAI ACCGGT 1 cut(s) 155
PkrI GCNGC 3 cut(s) 583, 732, 841
PleI GAGTC 4 cut(s) 55, 336, 511, 822
PpsI GAGTC 4 cut(s) 55, 336, 511, 822
Psp124BI GAGCTC 1 cut(s) 740
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PstI CTGCAG 2 cut(s) 735, 813
RsaI GTAC 1 cut(s) 760
RsaNI GTAC 1 cut(s) 759
RseI CAYNNNNRTG 2 cut(s) 186, 279
SacI GAGCTC 1 cut(s) 740
SaqAI TTAA 3 cut(s) 228, 354, 792
SatI GCNGC 3 cut(s) 582, 731, 840
Sau3AI GATC 4 cut(s) 160, 361, 421, 772
SchI GAGTC 4 cut(s) 56, 337, 511, 823
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 2 cut(s) 740, 808
SexAI ACCWGGT 1 cut(s) 64
SfcI CTRYAG 2 cut(s) 731, 809
SmiMI CAYNNNNRTG 2 cut(s) 186, 279
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 3 cut(s) 236, 351, 548
SseBI AGGCCT 1 cut(s) 95
SspMI CTAG 2 cut(s) 375, 642
SstI GAGCTC 1 cut(s) 740
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 280
TaiI ACGT 1 cut(s) 580
TasI AATT 3 cut(s) 236, 351, 548
TfiI GAWTC 4 cut(s) 131, 371, 401, 533
Tru1I TTAA 3 cut(s) 228, 354, 792
Tru9I TTAA 3 cut(s) 228, 354, 792
TscAI CASTG 2 cut(s) 454, 813
TseI GCWGC 3 cut(s) 581, 730, 839
TspDTI ATGAA 6 cut(s) 29, 411, 426, 474, 507, 560
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 2 cut(s) 454, 813
VneI GTGCAC 1 cut(s) 804
XagI CCTNNNNNAGG 1 cut(s) 781
XapI RAATTY 1 cut(s) 236
XmnI GAANNNNTTC 2 cut(s) 130, 551
XspI CTAG 2 cut(s) 375, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.