Rroxscaffold_6G00411450

Regulated-SNARE-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011845
Physical Location & Seq
Reverse (-)
33974271 .. 33977646
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00411450.1

Sequence Viewer

Length: 429 bp
ATGAGTGTCTATCCCAAACAAAAGCTAACCCAGCGCACTCTTCTAGAGTTGAACTTCTGCCCAGTTTCGAAAATTCAAACTTGTTTCTATGAATCTAAAGAACTTGGAACTGATTCAGCTCATGATGAGAGTCCATCCGAAACCGAATCAATAAGGATGAGATCAGTGAGGTGCCAGATTTATATCTTGGTGCAAGCATTCATCTCTCAAGAGACCCTAATAATCTTAAGGATCCCAATTCTACCCAGACTAAAGCTGAAGGAGCACATGAAGTACTGTGTGGATCATCCTGAAGAGATCAACAAGCTAGCAAAATTGAAGGCTCACGTTACTGAGGTCAAGGGTGTTATGATGGAAAATATTGAGAAGGTTCTTGACCGTGGTGAGAAGATTGAGCTATTGGTGGATAAGACTGATAACCCAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

142

Amino Acids

16.41

Weight (kDa)

6.9

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Synaptobrevin PF00957 102 - 140 2.1e-12 Synaptobrevin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 171
AclWI GGATC 3 cut(s) 226, 239, 291
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 2 cut(s) 278, 312
AfaI GTAC 1 cut(s) 275
AflII CTTAAG 1 cut(s) 226
AgsI TTSAA 3 cut(s) 52, 77, 319
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 5 cut(s) 25, 119, 256, 307, 397
AluI AGCT 5 cut(s) 25, 119, 256, 307, 397
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 3 cut(s) 226, 239, 291
ApoI RAATTY 1 cut(s) 72
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 36
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 68
AsuNHI GCTAGC 1 cut(s) 307
BamHI GGATCC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 267
BccI CCATC 2 cut(s) 142, 346
BciT130I CCWGG 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 44, 308
BfrI CTTAAG 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 423
BmiI GGNNCC 2 cut(s) 173, 233
BmrFI CCNGG 1 cut(s) 423
BmrI ACTGGG 1 cut(s) 56
BmtI GCTAGC 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 379, 421
Bse1I ACTGG 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 182
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 379, 421
BseGI GGATG 3 cut(s) 134, 162, 286
BseJI GATNNNNATC 1 cut(s) 182
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 4 cut(s) 161, 231, 283, 297
BspCNI CTCAG 1 cut(s) 325
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 173, 233
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 3 cut(s) 226, 239, 291
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 171
BspTI CTTAAG 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 206
BsrI ACTGG 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 379, 421
BssMI GATC 4 cut(s) 161, 231, 283, 297
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 2 cut(s) 278, 380
Bst6I CTCTTC 2 cut(s) 45, 288
BstAFI CTTAAG 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 195, 309
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 3 cut(s) 134, 162, 286
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 4 cut(s) 164, 234, 286, 300
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 4 cut(s) 161, 231, 283, 297
BstMWI GCNNNNNNNGC 2 cut(s) 31, 262
BstNI CCWGG 1 cut(s) 423
BstSCI CCNGG 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 3 cut(s) 134, 162, 286
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 2 cut(s) 195, 309
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 274
CviAII CATG 2 cut(s) 122, 268
CviJI RGCY 7 cut(s) 25, 119, 256, 307, 323, 397, 426
CviKI_1 RGCY 7 cut(s) 25, 119, 256, 307, 323, 397, 426
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 333
DpnI GATC 4 cut(s) 163, 233, 285, 299
DpnII GATC 4 cut(s) 161, 231, 283, 297
Eam1104I CTCTTC 2 cut(s) 45, 288
EarI CTCTTC 2 cut(s) 45, 288
Eco31I GGTCTC 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 278, 312
EcoRII CCWGG 1 cut(s) 421
FaeI CATG 2 cut(s) 125, 271
FaiI YATR 5 cut(s) 90, 123, 183, 269, 350
FatI CATG 2 cut(s) 121, 267
FokI GGATG 3 cut(s) 121, 169, 273
FspBI CTAG 2 cut(s) 44, 308
GlaI GCGC 1 cut(s) 35
GsaI CCCAGC 1 cut(s) 34
HhaI GCGC 1 cut(s) 36
Hin1II CATG 2 cut(s) 125, 271
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HinfI GANTC 4 cut(s) 92, 113, 130, 146
HphI GGTGA 1 cut(s) 395
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 5 cut(s) 44, 122, 209, 290, 374
HpyAV CCTTC 3 cut(s) 253, 313, 361
HpyCH4III ACNGT 2 cut(s) 278, 380
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 193
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 262
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 2 cut(s) 125, 271
HspAI GCGC 1 cut(s) 34
Kzo9I GATC 4 cut(s) 161, 231, 283, 297
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 6 cut(s) 44, 75, 188, 259, 303, 408
MaeI CTAG 2 cut(s) 44, 308
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 328
MalI GATC 4 cut(s) 163, 233, 285, 299
MboI GATC 4 cut(s) 161, 231, 283, 297
MboII GAAGA 3 cut(s) 32, 305, 400
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 3 cut(s) 72, 237, 314
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 2 cut(s) 162, 328
MroXI GAANNNNTTC 1 cut(s) 112
MseI TTAA 1 cut(s) 227
MspCI CTTAAG 1 cut(s) 226
MspR9I CCNGG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 2 cut(s) 31, 262
NdeII GATC 4 cut(s) 161, 231, 283, 297
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 125, 271
NlaIV GGNNCC 2 cut(s) 173, 233
NspV TTCGAA 1 cut(s) 68
PagI TCATGA 1 cut(s) 121
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 92, 113, 146
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 173, 233
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 227
Sau3AI GATC 4 cut(s) 161, 231, 283, 297
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 27, 121, 173, 258, 309, 330, 339, 372, 399
SfuI TTCGAA 1 cut(s) 68
SmlI CTYRAG 2 cut(s) 207, 226
SmoI CTYRAG 2 cut(s) 207, 226
Sse9I AATT 3 cut(s) 72, 237, 314
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 44, 308
StyD4I CCNGG 1 cut(s) 421
TaaI ACNGT 2 cut(s) 278, 380
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 68
TasI AATT 3 cut(s) 72, 237, 314
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 3 cut(s) 92, 113, 146
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 1 cut(s) 171
TspDTI ATGAA 3 cut(s) 105, 190, 284
TspRI CASTG 1 cut(s) 171
Vha464I CTTAAG 1 cut(s) 226
XapI RAATTY 1 cut(s) 72
XbaI TCTAGA 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 2 cut(s) 44, 308
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.