Rh6DG223500

Regulated-SNARE-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011845
Physical Location & Seq
Forward (+)
40020369 .. 40053349
32981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG223500.1

Sequence Viewer

Length: 474 bp
ATGGACGCAGAAGAGCTAGGGCTAGCGATGCTTTTCTTCGACTTTAAGCGGTCCCACGGTCACAACTCACATGAGGGACCAAAGCTAACAGAGCACATGAAGTACTGTGTGGATCATCTTGAAGAGATAAACAAGCTTGCAAAAGTGAAGGCTCACGTTACTGAGGTCAAGGGTGTTATGATGGAAAATAGTGAGAAGGTTCTTGATCGTGGTGAGAAGATTGAGCAGTTGGTGGATAAGACTGATAACCCAGTAACAAAGAGAATACGAGATATCAGAAGAGAGCAAAGTGGTGCGAGACCGATAGGCCAGAGCAATGCAGCAACTCTTGATGAAGCGTCGTGGCCGTCCCCACCAAATACAACGCCGTCAGGACTCAATTCCGGCGTCTCAGTGAGCCCATACTCTTTTCGGAGAACGAGATATGGAAGGCGAGACAGGCTGAGGACAATTCTGGCCAAGCTGGAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

157

Amino Acids

17.8

Weight (kDa)

9.04

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Synaptobrevin PF00957 45 - 83 4.5e-11 Synaptobrevin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 49
AclWI GGATC 1 cut(s) 120
AcoI YGGCCR 2 cut(s) 344, 456
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 1 cut(s) 104
AgsI TTSAA 1 cut(s) 122
AjiI CACGTC 1 cut(s) 471
AluBI AGCT 4 cut(s) 16, 85, 136, 463
AluI AGCT 4 cut(s) 16, 85, 136, 463
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 4 cut(s) 292, 394, 429, 461
AlwI GGATC 1 cut(s) 120
AoxI GGCC 3 cut(s) 307, 344, 456
ApeKI GCWGC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 51, 77
AsuHPI GGTGA 1 cut(s) 224
AsuNHI GCTAGC 1 cut(s) 22
AvaII GGWCC 2 cut(s) 51, 77
BalI TGGCCA 1 cut(s) 458
BanII GRGCYC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 1 cut(s) 332
BccI CCATC 1 cut(s) 175
BceAI ACGGC 2 cut(s) 331, 352
BcoDI GTCTC 4 cut(s) 292, 394, 429, 461
BfaI CTAG 2 cut(s) 17, 23
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
BmcAI AGTACT 1 cut(s) 104
Bme18I GGWCC 2 cut(s) 51, 77
BmgBI CACGTC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 51, 77
BmiI GGNNCC 2 cut(s) 53, 78
BmrI ACTGGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 18
BmtI GCTAGC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 251
Bse3DI GCAATG 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 153, 405, 434
BseNI ACTGG 1 cut(s) 251
BseXI GCAGC 1 cut(s) 332
BshFI GGCC 3 cut(s) 309, 346, 458
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 384
BslFI GGGAC 3 cut(s) 37, 90, 334
BsmAI GTCTC 4 cut(s) 292, 394, 429, 461
BsmBI CGTCTC 2 cut(s) 394, 461
BsmFI GGGAC 3 cut(s) 37, 90, 334
BsnI GGCC 3 cut(s) 309, 346, 458
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 96, 401
Bsp143I GATC 2 cut(s) 112, 205
BspACI CCGC 1 cut(s) 49
BspANI GGCC 3 cut(s) 309, 346, 458
BspCNI CTCAG 3 cut(s) 154, 404, 435
BspLI GGNNCC 2 cut(s) 53, 78
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 120
BspQI GCTCTTC 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 322
BsrI ACTGG 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 2 cut(s) 112, 205
BssNI GRCGYC 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 59, 107
Bst6I CTCTTC 3 cut(s) 6, 117, 274
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 24, 138
BstDEI CTNAG 3 cut(s) 162, 391, 443
BstDSI CCRYGG 1 cut(s) 55
BstKTI GATC 2 cut(s) 115, 208
BstMAI GTCTC 4 cut(s) 292, 394, 429, 461
BstMBI GATC 2 cut(s) 112, 205
BstMWI GCNNNNNNNGC 3 cut(s) 28, 91, 439
BstV1I GCAGC 1 cut(s) 332
BsuRI GGCC 3 cut(s) 309, 346, 458
BtgI CCRYGG 1 cut(s) 55
BtgZI GCGATG 1 cut(s) 41
BtrI CACGTC 1 cut(s) 471
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 2 cut(s) 24, 138
Cfr13I GGNCC 2 cut(s) 51, 77
CseI GACGC 3 cut(s) 14, 327, 376
Csp6I GTAC 1 cut(s) 103
CviAII CATG 2 cut(s) 71, 97
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 3 cut(s) 162, 391, 443
DpnI GATC 2 cut(s) 114, 207
DpnII GATC 2 cut(s) 112, 205
EaeI YGGCCR 2 cut(s) 344, 456
Eam1104I CTCTTC 3 cut(s) 6, 117, 274
EarI CTCTTC 3 cut(s) 6, 117, 274
Eco24I GRGCYC 1 cut(s) 401
Eco31I GGTCTC 1 cut(s) 292
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 51, 77
EcoRV GATATC 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 401
Esp3I CGTCTC 2 cut(s) 394, 461
FaeI CATG 2 cut(s) 74, 100
FaiI YATR 5 cut(s) 72, 98, 179, 403, 426
FaqI GGGAC 3 cut(s) 37, 90, 334
FatI CATG 2 cut(s) 70, 96
Fnu4HI GCNGC 1 cut(s) 321
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 2 cut(s) 17, 23
GluI GCNGC 1 cut(s) 321
HaeIII GGCC 3 cut(s) 309, 346, 458
HapII CCGG 1 cut(s) 384
HgaI GACGC 3 cut(s) 14, 327, 376
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 2 cut(s) 74, 100
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 1 cut(s) 375
HpaII CCGG 1 cut(s) 384
HphI GGTGA 1 cut(s) 224
Hpy188I TCNGA 2 cut(s) 278, 414
Hpy188III TCNNGA 4 cut(s) 119, 203, 329, 372
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 3 cut(s) 142, 190, 423
HpyCH4III ACNGT 2 cut(s) 59, 107
HpyCH4IV ACGT 2 cut(s) 156, 470
HpyCH4V TGCA 2 cut(s) 140, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 91, 439
HpyF3I CTNAG 3 cut(s) 162, 391, 443
HpySE526I ACGT 2 cut(s) 156, 470
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 2 cut(s) 74, 100
Kzo9I GATC 2 cut(s) 112, 205
LguI GCTCTTC 1 cut(s) 6
LpnPI CCDG 7 cut(s) 264, 323, 357, 397, 424, 440, 449
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 1 cut(s) 18
MaeI CTAG 2 cut(s) 17, 23
MaeII ACGT 2 cut(s) 156, 470
MaeIII GTNAC 3 cut(s) 59, 157, 253
MalI GATC 2 cut(s) 114, 207
MboI GATC 2 cut(s) 112, 205
MboII GAAGA 5 cut(s) 23, 28, 134, 229, 291
MhlI GDGCHC 2 cut(s) 96, 401
MlsI TGGCCA 1 cut(s) 458
MluCI AATT 2 cut(s) 379, 450
MluNI TGGCCA 1 cut(s) 458
MlyI GAGTC 1 cut(s) 369
MnlI CCTC 3 cut(s) 67, 157, 438
Mox20I TGGCCA 1 cut(s) 458
MscI TGGCCA 1 cut(s) 458
MseI TTAA 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 458
MspI CCGG 1 cut(s) 384
MwoI GCNNNNNNNGC 3 cut(s) 28, 91, 439
NdeII GATC 2 cut(s) 112, 205
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 2 cut(s) 74, 100
NlaIV GGNNCC 2 cut(s) 53, 78
NmuCI GTSAC 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 6
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 53, 78
PspPI GGNCC 2 cut(s) 51, 77
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 2 cut(s) 112, 205
Sau96I GGNCC 2 cut(s) 51, 77
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 1 cut(s) 369
SduI GDGCHC 2 cut(s) 96, 401
SetI ASST 8 cut(s) 18, 87, 138, 159, 168, 201, 465, 473
SfaNI GCATC 1 cut(s) 18
SinI GGWCC 2 cut(s) 51, 77
Sse9I AATT 2 cut(s) 379, 450
SsiI CCGC 1 cut(s) 49
SspMI CTAG 2 cut(s) 17, 23
TaaI ACNGT 2 cut(s) 59, 107
TaiI ACGT 2 cut(s) 159, 473
TaqI TCGA 1 cut(s) 39
TaqII GACCGA 1 cut(s) 316
TasI AATT 2 cut(s) 379, 450
TatI WGTACW 1 cut(s) 102
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 2 cut(s) 113, 348
TspRI CASTG 1 cut(s) 399
VpaK11BI GGWCC 2 cut(s) 51, 77
XspI CTAG 2 cut(s) 17, 23
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.