Rorug01G0124300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015744
Physical Location & Seq
Reverse (-)
21369163 .. 21372927
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0124300.1

Sequence Viewer

Length: 939 bp
ATGGTAGGTGCCAAGGGTCCTCAAGAGTCAAGACTCAAGAGGTTTAGTCTACCTGGCACGCTTCCACCTCAACTGGTGAAACTACATTACCTTAGAGAAATTGATTTGGTTTACAATTATCTTAGTGGTACAATACCAGCAGAATGGGGTTCAATGCAGTTAACTTCAATGAGGATTAGTGATATAAATGGAACAGAGCAGGATTTTCCTATGTTGAAAAACATGACTGGCCTAGTCAAATTGATACTGAGAAACTGTAACATTTCTGGAGAGATCCCTCCTTACCTATGGACACTGCAGAAACTGGAAATGCTGGATCTGAGTTTTAACAAGTTGATAGGAGAAATTCCAACAACAATCAGCTTAAAGCGTTTGAGATTCCTATTCTTAACTGGCAACTCTCTGAGAGGAAGTGTACCAGACTCAATCTTCATAGATGGAAGCAATGTGTTGAGGACTAGCAAAAGAAAAATCAAAAGGCCAATAAAGTACATTAACTACCGGGATTATACACGGGCAAATCACATAGTTCCAGATGGAACTTTGTTTTGCTTCTTTCTTTTGCCTCTGCCAAAATTCATGATTTGCCTTAAGAAAATCTGTGTTAGTTATTATGGTATAGTGGAGATATGTAGGATGCTATTGATAGCGTATCAAAGGCTTCTCAAACAAGTGACTTTTGGCGACGGCAAAAATCTTCCGACGCCGTTGGTGGCGTCGCCAATGGTATTTGCCACGGCAATTCGCCGTCGCAAAATTATTGCGACGGCAAACGTTTTGCCGTCGCTAATAGTCTTGCGACGGCGAATTGCCGTGGCAAATACCATTGGCGACGCCACCAACGGCGTCGGAAGAATTGCCGTCGCAGAGCCGTCGCCAATGCTCTTTTGCGACGGCAAAAGAGGCTTTTGCTACGGCATTGCGCCGTGGCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

35.14

Weight (kDa)

9.97

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 77 - 116 7.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 49
AclI AACGTT 1 cut(s) 774
AclWI GGATC 2 cut(s) 268, 324
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 2 cut(s) 345, 575
AcyI GRCGYC 4 cut(s) 704, 716, 834, 846
AfaI GTAC 3 cut(s) 130, 417, 491
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 3 cut(s) 153, 168, 217
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 1 cut(s) 363
AluI AGCT 1 cut(s) 363
AlwI GGATC 2 cut(s) 268, 324
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 3 cut(s) 229, 479, 929
ApoI RAATTY 2 cut(s) 345, 575
AspLEI GCGC 1 cut(s) 925
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 1 cut(s) 88
AvaII GGWCC 1 cut(s) 17
BalI TGGCCA 1 cut(s) 931
BanI GGYRCC 1 cut(s) 8
BccI CCATC 2 cut(s) 431, 530
BcgI CGANNNNNNTGC 2 cut(s) 855, 889
BciT130I CCWGG 1 cut(s) 54
BcnI CCSGG 1 cut(s) 503
BfaI CTAG 2 cut(s) 233, 459
BfmI CTRYAG 1 cut(s) 296
BfrI CTTAAG 1 cut(s) 590
Bme1390I CCNGG 2 cut(s) 54, 503
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 10, 18
BmrFI CCNGG 2 cut(s) 54, 503
BmsI GCATC 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 6, 20
BpuMI CCSGG 1 cut(s) 503
BsaHI GRCGYC 4 cut(s) 704, 716, 834, 846
BsaJI CCNNGG 4 cut(s) 12, 735, 813, 926
Bse1I ACTGG 4 cut(s) 78, 232, 309, 397
Bse3DI GCAATG 2 cut(s) 451, 918
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 4 cut(s) 12, 735, 813, 926
BseGI GGATG 1 cut(s) 642
BseMI GCAATG 2 cut(s) 451, 918
BseMII CTCAG 3 cut(s) 239, 311, 395
BseNI ACTGG 4 cut(s) 78, 232, 309, 397
BshFI GGCC 3 cut(s) 231, 481, 931
BshNI GGYRCC 1 cut(s) 8
BsiSI CCGG 1 cut(s) 502
BsnI GGCC 3 cut(s) 231, 481, 931
Bsp143I GATC 2 cut(s) 273, 316
BspANI GGCC 3 cut(s) 231, 481, 931
BspCNI CTCAG 3 cut(s) 240, 312, 396
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 2 cut(s) 10, 18
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 2 cut(s) 268, 324
BspT107I GGYRCC 1 cut(s) 8
BspTI CTTAAG 1 cut(s) 590
BsrDI GCAATG 2 cut(s) 451, 918
BsrI ACTGG 4 cut(s) 78, 232, 309, 397
BssECI CCNNGG 4 cut(s) 12, 735, 813, 926
BssMI GATC 2 cut(s) 273, 316
BssNI GRCGYC 4 cut(s) 704, 716, 834, 846
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 257
BstACI GRCGYC 4 cut(s) 704, 716, 834, 846
BstAFI CTTAAG 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 5 cut(s) 92, 122, 248, 320, 404
BstDSI CCRYGG 3 cut(s) 735, 813, 926
BstF5I GGATG 1 cut(s) 642
BstHHI GCGC 1 cut(s) 925
BstKTI GATC 2 cut(s) 276, 319
BstMBI GATC 2 cut(s) 273, 316
BstMWI GCNNNNNNNGC 1 cut(s) 903
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 2 cut(s) 52, 501
BstSFI CTRYAG 1 cut(s) 296
BstX2I RGATCY 2 cut(s) 273, 316
BstXI CCANNNNNNTGG 1 cut(s) 144
BstYI RGATCY 2 cut(s) 273, 316
BsuRI GGCC 3 cut(s) 231, 481, 931
BtgI CCRYGG 3 cut(s) 735, 813, 926
BtsCI GGATG 1 cut(s) 642
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 59
CaiI CAGNNNCTG 1 cut(s) 304
CciI TCATGA 1 cut(s) 579
CfoI GCGC 1 cut(s) 925
Cfr13I GGNCC 1 cut(s) 17
CseI GACGC 4 cut(s) 705, 712, 835, 842
Csp6I GTAC 3 cut(s) 129, 416, 490
CviAII CATG 2 cut(s) 223, 580
CviJI RGCY 7 cut(s) 231, 363, 481, 661, 871, 906, 931
CviKI_1 RGCY 7 cut(s) 231, 363, 481, 661, 871, 906, 931
CviQI GTAC 3 cut(s) 129, 416, 490
DdeI CTNAG 5 cut(s) 92, 122, 248, 320, 404
DpnI GATC 2 cut(s) 275, 318
DpnII GATC 2 cut(s) 273, 316
EaeI YGGCCR 1 cut(s) 929
Eco130I CCWWGG 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 2 cut(s) 226, 583
FatI CATG 2 cut(s) 222, 579
FblI GTMKAC 1 cut(s) 49
FokI GGATG 1 cut(s) 649
FspBI CTAG 2 cut(s) 233, 459
GlaI GCGC 1 cut(s) 924
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 3 cut(s) 231, 481, 931
HapII CCGG 1 cut(s) 502
HgaI GACGC 4 cut(s) 705, 712, 835, 842
HhaI GCGC 1 cut(s) 925
Hin1I GRCGYC 4 cut(s) 704, 716, 834, 846
Hin1II CATG 2 cut(s) 226, 583
Hin6I GCGC 1 cut(s) 923
HinP1I GCGC 1 cut(s) 923
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 4 cut(s) 26, 33, 378, 422
HpaI GTTAAC 1 cut(s) 162
HpaII CCGG 1 cut(s) 502
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 4 cut(s) 50, 112, 162, 416
Hpy188I TCNGA 4 cut(s) 321, 405, 702, 851
Hpy188III TCNNGA 6 cut(s) 23, 30, 37, 267, 533, 580
Hpy8I GTNNAC 4 cut(s) 50, 112, 162, 416
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4IV ACGT 1 cut(s) 774
HpyCH4V TGCA 2 cut(s) 157, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 903
HpyF3I CTNAG 5 cut(s) 92, 122, 248, 320, 404
HpySE526I ACGT 1 cut(s) 774
Hsp92I GRCGYC 4 cut(s) 704, 716, 834, 846
Hsp92II CATG 2 cut(s) 226, 583
HspAI GCGC 1 cut(s) 923
KspAI GTTAAC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 273, 316
LweI GCATC 1 cut(s) 627
MaeI CTAG 2 cut(s) 233, 459
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 2 cut(s) 257, 673
MalI GATC 2 cut(s) 275, 318
MboI GATC 2 cut(s) 273, 316
MboII GAAGA 3 cut(s) 421, 689, 864
MflI RGATCY 2 cut(s) 273, 316
MlsI TGGCCA 1 cut(s) 931
MluCI AATT 9 cut(s) 99, 115, 239, 345, 575, 741, 756, 807, 855
MluNI TGGCCA 1 cut(s) 931
MlyI GAGTC 3 cut(s) 27, 35, 416
MmeI TCCRAC 3 cut(s) 374, 725, 829
MnlI CCTC 9 cut(s) 30, 33, 78, 165, 288, 401, 447, 576, 896
Mox20I TGGCCA 1 cut(s) 931
MscI TGGCCA 1 cut(s) 931
MseI TTAA 6 cut(s) 161, 327, 365, 389, 495, 591
Msp20I TGGCCA 1 cut(s) 931
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 1 cut(s) 502
MspR9I CCNGG 2 cut(s) 54, 503
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 903
NciI CCSGG 1 cut(s) 503
NdeII GATC 2 cut(s) 273, 316
NlaIII CATG 2 cut(s) 226, 583
NlaIV GGNNCC 2 cut(s) 10, 18
NmuCI GTSAC 1 cut(s) 673
PagI TCATGA 1 cut(s) 579
PfeI GAWTC 1 cut(s) 378
PleI GAGTC 3 cut(s) 27, 34, 416
PpsI GAGTC 3 cut(s) 27, 34, 416
PpuMI RGGWCCY 1 cut(s) 17
Psp1406I AACGTT 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 10, 18
PspPI GGNCC 1 cut(s) 17
PspPPI RGGWCCY 1 cut(s) 17
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 304
PsuI RGATCY 2 cut(s) 273, 316
RsaI GTAC 3 cut(s) 130, 417, 491
RsaNI GTAC 3 cut(s) 129, 416, 490
SaqAI TTAA 6 cut(s) 161, 327, 365, 389, 495, 591
Sau3AI GATC 2 cut(s) 273, 316
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 3 cut(s) 27, 35, 416
ScrFI CCNGG 2 cut(s) 54, 503
SetI ASST 8 cut(s) 10, 44, 55, 70, 93, 288, 365, 777
SfaNI GCATC 1 cut(s) 627
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 1 cut(s) 17
SmlI CTYRAG 3 cut(s) 21, 35, 590
SmoI CTYRAG 3 cut(s) 21, 35, 590
Sse9I AATT 9 cut(s) 99, 115, 239, 345, 575, 741, 756, 807, 855
SspMI CTAG 2 cut(s) 233, 459
StyD4I CCNGG 2 cut(s) 52, 501
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 257
TaiI ACGT 1 cut(s) 777
TasI AATT 9 cut(s) 99, 115, 239, 345, 575, 741, 756, 807, 855
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 6 cut(s) 161, 327, 365, 389, 495, 591
Tru9I TTAA 6 cut(s) 161, 327, 365, 389, 495, 591
TscAI CASTG 1 cut(s) 300
TseFI GTSAC 1 cut(s) 673
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 2 cut(s) 421, 568
TspRI CASTG 1 cut(s) 300
Vha464I CTTAAG 1 cut(s) 590
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 345, 575
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 2 cut(s) 233, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.