No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 360 |
| AarI | CACCTGC | 1 cut(s) | 1504 |
| Acc16I | TGCGCA | 1 cut(s) | 587 |
| Acc36I | ACCTGC | 1 cut(s) | 1504 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1215 |
| AccBSI | CCGCTC | 1 cut(s) | 904 |
| AccI | GTMKAC | 1 cut(s) | 697 |
| AccII | CGCG | 1 cut(s) | 302 |
| AciI | CCGC | 4 cut(s) | 300, 904, 982, 1031 |
| AclI | AACGTT | 1 cut(s) | 1079 |
| AclWI | GGATC | 4 cut(s) | 177, 650, 775, 922 |
| AcsI | RAATTY | 2 cut(s) | 58, 383 |
| AcuI | CTGAAG | 2 cut(s) | 501, 939 |
| AfaI | GTAC | 3 cut(s) | 709, 871, 1339 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1037, 1058, 1215, 1415 |
| AflII | CTTAAG | 1 cut(s) | 329 |
| AgsI | TTSAA | 7 cut(s) | 57, 112, 265, 547, 691, 1084, 1375 |
| AjiI | CACGTC | 1 cut(s) | 344 |
| AjnI | CCWGG | 2 cut(s) | 802, 1590 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1398, 1430 |
| Alw26I | GTCTC | 6 cut(s) | 187, 334, 608, 997, 1161, 1484 |
| AlwI | GGATC | 4 cut(s) | 177, 650, 775, 922 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 443, 1103 |
| Ama87I | CYCGRG | 1 cut(s) | 147 |
| ApeKI | GCWGC | 3 cut(s) | 391, 797, 896 |
| ApoI | RAATTY | 2 cut(s) | 58, 383 |
| AspA2I | CCTAGG | 1 cut(s) | 1052 |
| AspLEI | GCGC | 2 cut(s) | 588, 719 |
| AspS9I | GGNCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| AsuC2I | CCSGG | 2 cut(s) | 349, 1366 |
| AsuHPI | GGTGA | 1 cut(s) | 1339 |
| AvaI | CYCGRG | 1 cut(s) | 147 |
| AvaII | GGWCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| AvrII | CCTAGG | 1 cut(s) | 1052 |
| AxyI | CCTNAGG | 1 cut(s) | 102 |
| BanII | GRGCYC | 1 cut(s) | 475 |
| BbvI | GCAGC | 3 cut(s) | 403, 784, 908 |
| BccI | CCATC | 8 cut(s) | 194, 212, 418, 472, 874, 1277, 1448, 1545 |
| BciT130I | CCWGG | 2 cut(s) | 804, 1592 |
| BcnI | CCSGG | 2 cut(s) | 349, 1366 |
| BcoDI | GTCTC | 6 cut(s) | 187, 334, 608, 997, 1161, 1484 |
| BfaI | CTAG | 5 cut(s) | 131, 782, 1053, 1316, 1344 |
| BfmI | CTRYAG | 1 cut(s) | 568 |
| BfoI | RGCGCY | 1 cut(s) | 720 |
| BfrI | CTTAAG | 1 cut(s) | 329 |
| BfuAI | ACCTGC | 1 cut(s) | 1504 |
| BisI | GCNGC | 4 cut(s) | 392, 798, 897, 1031 |
| BlnI | CCTAGG | 1 cut(s) | 1052 |
| BlsI | GCNGC | 4 cut(s) | 393, 799, 898, 1032 |
| BmcAI | AGTACT | 1 cut(s) | 871 |
| Bme1390I | CCNGG | 4 cut(s) | 349, 804, 1366, 1592 |
| Bme18I | GGWCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| BmeT110I | CYCGRG | 1 cut(s) | 147 |
| BmgBI | CACGTC | 1 cut(s) | 344 |
| BmgT120I | GGNCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| BmiI | GGNNCC | 4 cut(s) | 223, 462, 678, 1596 |
| BmrFI | CCNGG | 4 cut(s) | 349, 804, 1366, 1592 |
| BmsI | GCATC | 4 cut(s) | 627, 837, 883, 1103 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 977, 1009 |
| BpmI | CTGGAG | 3 cut(s) | 695, 786, 1574 |
| BpuEI | CTTGAG | 2 cut(s) | 302, 696 |
| BpuMI | CCSGG | 2 cut(s) | 349, 1366 |
| BsaI | GGTCTC | 1 cut(s) | 187 |
| BsaJI | CCNNGG | 2 cut(s) | 1052, 1365 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 411, 441 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1037, 1058, 1215, 1415 |
| Bse1I | ACTGG | 1 cut(s) | 1004 |
| Bse21I | CCTNAGG | 1 cut(s) | 102 |
| Bse3DI | GCAATG | 1 cut(s) | 1541 |
| BseBI | CCWGG | 2 cut(s) | 804, 1592 |
| BseDI | CCNNGG | 2 cut(s) | 1052, 1365 |
| BseGI | GGATG | 3 cut(s) | 519, 847, 1537 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1037, 1058, 1215, 1415 |
| BseMI | GCAATG | 1 cut(s) | 1541 |
| BseMII | CTCAG | 2 cut(s) | 116, 296 |
| BseNI | ACTGG | 1 cut(s) | 1004 |
| BseRI | GAGGAG | 3 cut(s) | 90, 352, 1583 |
| BseXI | GCAGC | 3 cut(s) | 403, 784, 908 |
| Bsh1236I | CGCG | 1 cut(s) | 302 |
| BsiHKCI | CYCGRG | 1 cut(s) | 147 |
| BsiSI | CCGG | 3 cut(s) | 348, 680, 1365 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1037, 1058, 1215, 1415 |
| BsmAI | GTCTC | 6 cut(s) | 187, 334, 608, 997, 1161, 1484 |
| BsmBI | CGTCTC | 1 cut(s) | 334 |
| BsmI | GAATGC | 2 cut(s) | 583, 1033 |
| Bso31I | GGTCTC | 1 cut(s) | 187 |
| BsoBI | CYCGRG | 1 cut(s) | 147 |
| Bsp1286I | GDGCHC | 1 cut(s) | 475 |
| Bsp143I | GATC | 4 cut(s) | 182, 655, 767, 914 |
| BspACI | CCGC | 4 cut(s) | 300, 904, 982, 1031 |
| BspCNI | CTCAG | 2 cut(s) | 115, 295 |
| BspFNI | CGCG | 1 cut(s) | 302 |
| BspLI | GGNNCC | 4 cut(s) | 223, 462, 678, 1596 |
| BspMI | ACCTGC | 1 cut(s) | 1504 |
| BspPI | GGATC | 4 cut(s) | 177, 650, 775, 922 |
| BspTI | CTTAAG | 1 cut(s) | 329 |
| BspTNI | GGTCTC | 1 cut(s) | 187 |
| BsrBI | CCGCTC | 1 cut(s) | 904 |
| BsrDI | GCAATG | 1 cut(s) | 1541 |
| BsrI | ACTGG | 1 cut(s) | 1004 |
| BssECI | CCNNGG | 2 cut(s) | 1052, 1365 |
| BssMI | GATC | 4 cut(s) | 182, 655, 767, 914 |
| BssT1I | CCWWGG | 1 cut(s) | 1052 |
| Bst2UI | CCWGG | 2 cut(s) | 804, 1592 |
| Bst4CI | ACNGT | 6 cut(s) | 68, 374, 524, 702, 943, 1477 |
| Bst6I | CTCTTC | 3 cut(s) | 477, 500, 606 |
| BstAFI | CTTAAG | 1 cut(s) | 329 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1100 |
| BstC8I | GCNNGC | 3 cut(s) | 642, 826, 1609 |
| BstDEI | CTNAG | 4 cut(s) | 102, 282, 499, 947 |
| BstF5I | GGATG | 3 cut(s) | 519, 847, 1537 |
| BstFNI | CGCG | 1 cut(s) | 302 |
| BstH2I | RGCGCY | 1 cut(s) | 720 |
| BstHHI | GCGC | 2 cut(s) | 588, 719 |
| BstKTI | GATC | 4 cut(s) | 185, 658, 770, 917 |
| BstMAI | GTCTC | 6 cut(s) | 187, 334, 608, 997, 1161, 1484 |
| BstMBI | GATC | 4 cut(s) | 182, 655, 767, 914 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 20, 821, 1100 |
| BstNI | CCWGG | 2 cut(s) | 804, 1592 |
| BstNSI | RCATGY | 1 cut(s) | 398 |
| BstSCI | CCNGG | 4 cut(s) | 347, 802, 1364, 1590 |
| BstSFI | CTRYAG | 1 cut(s) | 568 |
| BstUI | CGCG | 1 cut(s) | 302 |
| BstV1I | GCAGC | 3 cut(s) | 403, 784, 908 |
| BstX2I | RGATCY | 3 cut(s) | 182, 655, 914 |
| BstYI | RGATCY | 3 cut(s) | 182, 655, 914 |
| Bsu36I | CCTNAGG | 1 cut(s) | 102 |
| BtrI | CACGTC | 1 cut(s) | 344 |
| BtsCI | GGATG | 3 cut(s) | 519, 847, 1537 |
| BtsI | GCAGTG | 2 cut(s) | 581, 595 |
| BtsIMutI | CAGTG | 5 cut(s) | 581, 595, 936, 1011, 1278 |
| BveI | ACCTGC | 1 cut(s) | 1504 |
| Cac8I | GCNNGC | 3 cut(s) | 642, 826, 1609 |
| CaiI | CAGNNNCTG | 2 cut(s) | 443, 1103 |
| CfoI | GCGC | 2 cut(s) | 588, 719 |
| Cfr13I | GGNCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| Csp6I | GTAC | 3 cut(s) | 708, 870, 1338 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1159, 1194 |
| CviAII | CATG | 3 cut(s) | 395, 433, 468 |
| CviQI | GTAC | 3 cut(s) | 708, 870, 1338 |
| DdeI | CTNAG | 4 cut(s) | 102, 282, 499, 947 |
| DpnI | GATC | 4 cut(s) | 184, 657, 769, 916 |
| DpnII | GATC | 4 cut(s) | 182, 655, 767, 914 |
| Eam1104I | CTCTTC | 3 cut(s) | 477, 500, 606 |
| EarI | CTCTTC | 3 cut(s) | 477, 500, 606 |
| Eco130I | CCWWGG | 1 cut(s) | 1052 |
| Eco24I | GRGCYC | 1 cut(s) | 475 |
| Eco31I | GGTCTC | 1 cut(s) | 187 |
| Eco47I | GGWCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| Eco57I | CTGAAG | 2 cut(s) | 501, 939 |
| Eco81I | CCTNAGG | 1 cut(s) | 102 |
| Eco88I | CYCGRG | 1 cut(s) | 147 |
| EcoO109I | RGGNCCY | 2 cut(s) | 222, 1594 |
| EcoRII | CCWGG | 2 cut(s) | 802, 1590 |
| EcoT14I | CCWWGG | 1 cut(s) | 1052 |
| EcoT22I | ATGCAT | 1 cut(s) | 1096 |
| EcoT38I | GRGCYC | 1 cut(s) | 475 |
| ErhI | CCWWGG | 1 cut(s) | 1052 |
| Esp3I | CGTCTC | 1 cut(s) | 334 |
| FaeI | CATG | 3 cut(s) | 398, 436, 471 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 474, 506, 705, 737 |
| FatI | CATG | 3 cut(s) | 394, 432, 467 |
| FauI | CCCGC | 1 cut(s) | 975 |
| FauNDI | CATATG | 1 cut(s) | 529 |
| FblI | GTMKAC | 1 cut(s) | 697 |
| Fnu4HI | GCNGC | 4 cut(s) | 392, 798, 897, 1031 |
| FokI | GGATG | 3 cut(s) | 526, 854, 1524 |
| FriOI | GRGCYC | 1 cut(s) | 475 |
| Fsp4HI | GCNGC | 4 cut(s) | 392, 798, 897, 1031 |
| FspBI | CTAG | 5 cut(s) | 131, 782, 1053, 1316, 1344 |
| FspI | TGCGCA | 1 cut(s) | 587 |
| GlaI | GCGC | 2 cut(s) | 587, 718 |
| GluI | GCNGC | 4 cut(s) | 392, 798, 897, 1031 |
| GsuI | CTGGAG | 3 cut(s) | 695, 786, 1574 |
| HaeII | RGCGCY | 1 cut(s) | 720 |
| HapII | CCGG | 3 cut(s) | 348, 680, 1365 |
| HhaI | GCGC | 2 cut(s) | 588, 719 |
| Hin1II | CATG | 3 cut(s) | 398, 436, 471 |
| Hin6I | GCGC | 2 cut(s) | 586, 717 |
| HinP1I | GCGC | 2 cut(s) | 586, 717 |
| HindIII | AAGCTT | 1 cut(s) | 326 |
| HinfI | GANTC | 7 cut(s) | 352, 487, 694, 1000, 1046, 1429, 1528 |
| HpaII | CCGG | 3 cut(s) | 348, 680, 1365 |
| HphI | GGTGA | 1 cut(s) | 1339 |
| Hpy166II | GTNNAC | 4 cut(s) | 593, 698, 953, 1330 |
| Hpy188I | TCNGA | 3 cut(s) | 919, 1453, 1560 |
| Hpy188III | TCNNGA | 8 cut(s) | 190, 377, 674, 691, 1014, 1166, 1265, 1532 |
| Hpy8I | GTNNAC | 4 cut(s) | 593, 698, 953, 1330 |
| HpyAV | CCTTC | 5 cut(s) | 39, 914, 1102, 1172, 1187 |
| HpyCH4III | ACNGT | 6 cut(s) | 68, 374, 524, 702, 943, 1477 |
| HpyCH4IV | ACGT | 3 cut(s) | 343, 1079, 1387 |
| HpyCH4V | TGCA | 9 cut(s) | 23, 173, 527, 581, 896, 1094, 1349, 1611, 1631 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 20, 821, 1100 |
| HpyF3I | CTNAG | 4 cut(s) | 102, 282, 499, 947 |
| HpySE526I | ACGT | 3 cut(s) | 343, 1079, 1387 |
| Hsp92II | CATG | 3 cut(s) | 398, 436, 471 |
| HspAI | GCGC | 2 cut(s) | 586, 717 |
| Kzo9I | GATC | 4 cut(s) | 182, 655, 767, 914 |
| LmnI | GCTCC | 2 cut(s) | 676, 805 |
| Lsp1109I | GCAGC | 3 cut(s) | 403, 784, 908 |
| LweI | GCATC | 4 cut(s) | 627, 837, 883, 1103 |
| MaeI | CTAG | 5 cut(s) | 131, 782, 1053, 1316, 1344 |
| MaeII | ACGT | 3 cut(s) | 343, 1079, 1387 |
| MaeIII | GTNAC | 3 cut(s) | 68, 906, 1471 |
| MalI | GATC | 4 cut(s) | 184, 657, 769, 916 |
| MbiI | CCGCTC | 1 cut(s) | 904 |
| MboI | GATC | 4 cut(s) | 182, 655, 767, 914 |
| MboII | GAAGA | 7 cut(s) | 24, 482, 487, 494, 623, 665, 1438 |
| MfeI | CAATTG | 2 cut(s) | 741, 855 |
| MflI | RGATCY | 3 cut(s) | 182, 655, 914 |
| MhlI | GDGCHC | 1 cut(s) | 475 |
| MluCI | AATT | 7 cut(s) | 58, 383, 741, 759, 855, 1116, 1197 |
| MlyI | GAGTC | 2 cut(s) | 361, 703 |
| MmeI | TCCRAC | 4 cut(s) | 397, 745, 1137, 1200 |
| Mph1103I | ATGCAT | 1 cut(s) | 1096 |
| MseI | TTAA | 6 cut(s) | 164, 330, 977, 1119, 1127, 1143 |
| MspA1I | CMGCKG | 2 cut(s) | 776, 797 |
| MspCI | CTTAAG | 1 cut(s) | 329 |
| MspI | CCGG | 3 cut(s) | 348, 680, 1365 |
| MspR9I | CCNGG | 4 cut(s) | 349, 804, 1366, 1592 |
| MunI | CAATTG | 2 cut(s) | 741, 855 |
| Mva1269I | GAATGC | 2 cut(s) | 583, 1033 |
| MvaI | CCWGG | 2 cut(s) | 804, 1592 |
| MvnI | CGCG | 1 cut(s) | 302 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 20, 821, 1100 |
| NciI | CCSGG | 2 cut(s) | 349, 1366 |
| NdeI | CATATG | 1 cut(s) | 529 |
| NdeII | GATC | 4 cut(s) | 182, 655, 767, 914 |
| NlaIII | CATG | 3 cut(s) | 398, 436, 471 |
| NlaIV | GGNNCC | 4 cut(s) | 223, 462, 678, 1596 |
| NsbI | TGCGCA | 1 cut(s) | 587 |
| NsiI | ATGCAT | 1 cut(s) | 1096 |
| NspI | RCATGY | 1 cut(s) | 398 |
| PaeR7I | CTCGAG | 1 cut(s) | 147 |
| PaqCI | CACCTGC | 1 cut(s) | 1504 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1261 |
| PctI | GAATGC | 2 cut(s) | 583, 1033 |
| PfeI | GAWTC | 5 cut(s) | 487, 1000, 1046, 1429, 1528 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1215 |
| PfoI | TCCNGGA | 2 cut(s) | 802, 1590 |
| PkrI | GCNGC | 4 cut(s) | 393, 799, 898, 1032 |
| PleI | GAGTC | 2 cut(s) | 360, 702 |
| PpsI | GAGTC | 2 cut(s) | 360, 702 |
| PpuMI | RGGWCCY | 2 cut(s) | 222, 1594 |
| PsiI | TTATAA | 1 cut(s) | 360 |
| Psp1406I | AACGTT | 1 cut(s) | 1079 |
| Psp5II | RGGWCCY | 2 cut(s) | 222, 1594 |
| Psp6I | CCWGG | 2 cut(s) | 802, 1590 |
| PspGI | CCWGG | 2 cut(s) | 802, 1590 |
| PspN4I | GGNNCC | 4 cut(s) | 223, 462, 678, 1596 |
| PspPI | GGNCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| PspPPI | RGGWCCY | 2 cut(s) | 222, 1594 |
| PspXI | VCTCGAGB | 1 cut(s) | 147 |
| PstNI | CAGNNNCTG | 2 cut(s) | 443, 1103 |
| PsuI | RGATCY | 3 cut(s) | 182, 655, 914 |
| PvuII | CAGCTG | 2 cut(s) | 776, 797 |
| RsaI | GTAC | 3 cut(s) | 709, 871, 1339 |
| RsaNI | GTAC | 3 cut(s) | 708, 870, 1338 |
| SaqAI | TTAA | 6 cut(s) | 164, 330, 977, 1119, 1127, 1143 |
| SatI | GCNGC | 4 cut(s) | 392, 798, 897, 1031 |
| Sau3AI | GATC | 4 cut(s) | 182, 655, 767, 914 |
| Sau96I | GGNCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| ScaI | AGTACT | 1 cut(s) | 871 |
| SchI | GAGTC | 2 cut(s) | 361, 703 |
| ScrFI | CCNGG | 4 cut(s) | 349, 804, 1366, 1592 |
| SduI | GDGCHC | 1 cut(s) | 475 |
| SfaNI | GCATC | 4 cut(s) | 627, 837, 883, 1103 |
| SfcI | CTRYAG | 1 cut(s) | 568 |
| Sfr274I | CTCGAG | 1 cut(s) | 147 |
| SinI | GGWCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| SlaI | CTCGAG | 1 cut(s) | 147 |
| SmlI | CTYRAG | 4 cut(s) | 147, 317, 329, 711 |
| SmoI | CTYRAG | 4 cut(s) | 147, 317, 329, 711 |
| Sse9I | AATT | 7 cut(s) | 58, 383, 741, 759, 855, 1116, 1197 |
| SsiI | CCGC | 4 cut(s) | 300, 904, 982, 1031 |
| SspI | AATATT | 1 cut(s) | 1359 |
| SspMI | CTAG | 5 cut(s) | 131, 782, 1053, 1316, 1344 |
| StyD4I | CCNGG | 4 cut(s) | 347, 802, 1364, 1590 |
| StyI | CCWWGG | 1 cut(s) | 1052 |
| TaaI | ACNGT | 6 cut(s) | 68, 374, 524, 702, 943, 1477 |
| TaiI | ACGT | 3 cut(s) | 346, 1082, 1390 |
| TaqI | TCGA | 6 cut(s) | 148, 227, 406, 667, 1165, 1264 |
| TaqII | GACCGA | 1 cut(s) | 211 |
| TasI | AATT | 7 cut(s) | 58, 383, 741, 759, 855, 1116, 1197 |
| TatI | WGTACW | 1 cut(s) | 869 |
| TauI | GCSGC | 1 cut(s) | 1033 |
| TfiI | GAWTC | 5 cut(s) | 487, 1000, 1046, 1429, 1528 |
| Tru1I | TTAA | 6 cut(s) | 164, 330, 977, 1119, 1127, 1143 |
| Tru9I | TTAA | 6 cut(s) | 164, 330, 977, 1119, 1127, 1143 |
| TscAI | CASTG | 5 cut(s) | 588, 595, 936, 1011, 1285 |
| TseI | GCWGC | 3 cut(s) | 391, 797, 896 |
| TspDTI | ATGAA | 1 cut(s) | 614 |
| TspGWI | ACGGA | 1 cut(s) | 271 |
| TspRI | CASTG | 5 cut(s) | 588, 595, 936, 1011, 1285 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1215 |
| Vha464I | CTTAAG | 1 cut(s) | 329 |
| VpaK11BI | GGWCC | 6 cut(s) | 222, 248, 809, 953, 1159, 1594 |
| XapI | RAATTY | 2 cut(s) | 58, 383 |
| XceI | RCATGY | 1 cut(s) | 398 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1216 |
| XhoI | CTCGAG | 1 cut(s) | 147 |
| XmaJI | CCTAGG | 1 cut(s) | 1052 |
| XmiI | GTMKAC | 1 cut(s) | 697 |
| XspI | CTAG | 5 cut(s) | 131, 782, 1053, 1316, 1344 |
| ZrmI | AGTACT | 1 cut(s) | 871 |
| Zsp2I | ATGCAT | 1 cut(s) | 1096 |