Rh1DG133300

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000450
Physical Location & Seq
Reverse (-)
27945200 .. 27957602
12403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG133300.1

Sequence Viewer

Length: 453 bp
ATGGTTGAAAAATGGCTGAAACCAAATTGGCAGGAAAGCTCGTACCAAAACTCGATAAATCATGGGAAGTCTGACATGGTGCATACAACCGGCTCAGTTCCAATGGCGAAATATATCAAAGATGAGCCTGTCATCCCAGGATACACACCACTATATGACACACTAATAGCTGATGAGGTTCTTAGAATGGGACTCATATGGTGGGAGTCAAGACATGGCTTAATTGACTGGCAGGTTGGGATCACCCGCAATCGAGGACCTTTGCTGTTTTTGGGACAGGGCTGGGATAGATTTTTCAGGGGGAACAAGCTGGCTGATAAGCGGTTTAATTTGTTCACACACAAGGGTGGTTTACATTTCTCTGTCCTTGTATTCCATAAAAGGAGGGGTGCCCAGATGATGAAGGTCAGCCAGTTCATCATGCTGCCCCCAGTTCAATCAGTTCCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

150

Amino Acids

17.43

Weight (kDa)

9.74

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000450)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20080 FvH4_1g23272 FvH4_1g24190 FvH4_1g24191 FvH4_2g16992 FvH4_4g20600 FvH4_5g37430 FvH4_6g13850 FvH4_6g23331 FvH4_7g07732
prunus_persica Prupe.2G062800_v2.0.a1 Prupe.7G020400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0159341 RchiOBHm_Chr3g0461151 RchiOBHm_Chr3g0471341 RchiOBHm_Chr5g0079051
rosa_laevigata RLG00000013771 RLG00000016694 RLG00000019616 RLG00000020143 RLG00000022642 RLG00000030165
rosa_multiflora Rmu_sc0000058.1_g000010 Rmu_sc0000898.1_g000045 Rmu_sc0001200.1_g000007 Rmu_sc0001200.1_g000008 Rmu_sc0001323.1_g000001 Rmu_sc0002194.1_g000008 Rmu_sc0002206.1_g000017 Rmu_sc0002230.1_g000001 Rmu_sc0002280.1_g000001 Rmu_sc0003047.1_g000022 Rmu_sc0003623.1_g000007 Rmu_sc0003835.1_g000012
rosa_roxburghii Rroxscaffold_1G00019590 Rroxscaffold_1G00029010 Rroxscaffold_1G00029250 Rroxscaffold_1G00031650 Rroxscaffold_1G00031660 Rroxscaffold_1G00052850 Rroxscaffold_2G00144000 Rroxscaffold_3G00231810 Rroxscaffold_3G00245070 Rroxscaffold_3G00268690 Rroxscaffold_4G00312920 Rroxscaffold_5G00350060 Rroxscaffold_6G00412590 Rroxscaffold_6G00412600 Rroxscaffold_6G00420320 Rroxscaffold_7G00192970 Rroxscaffold_7G00197810 Rroxscaffold_7G00208250
rosa_rugosa Rorug01G0074000 Rorug01G0273600.1 Rorug01G0273700 Rorug03G0275700 Rorug04G0060600 Rorug04G0063100 Rorug04G0170400 Rorug05G0185600 Rorug05G0224200 Rorug05G0234600
rosa_samantha Rh1CG227300 Rh1DG133300 Rh1DG133400 Rh2AG004600 Rh2BG038900 Rh3BG045400 Rh3BG359100 Rh3BG359200 Rh3CG044000 Rh3CG355700 Rh4CG188000 Rh4CG217400 Rh4DG018500 Rh4DG092800 Rh4DG128600 Rh4DG141700 Rh5CG305800 Rh5DG275100 Rh5DG430600 Rh6DG316100
rosa_wichuraiana Rw0G005510 Rw0G010440 Rw0G010450 Rw1G018670 Rw3G028280 Rw4G010530 Rw4G010930 Rw4G012110 Rw4G012720 Rw5G007170 Rw7G023020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 223
AccB1I GGYRCC 1 cut(s) 389
AciI CCGC 2 cut(s) 247, 322
AclWI GGATC 1 cut(s) 248
AfaI GTAC 1 cut(s) 44
AgsI TTSAA 2 cut(s) 8, 437
AjnI CCWGG 1 cut(s) 136
AleI CACNNNNGTG 1 cut(s) 345
AluBI AGCT 3 cut(s) 39, 170, 310
AluI AGCT 3 cut(s) 39, 170, 310
AlwI GGATC 1 cut(s) 248
ApeKI GCWGC 1 cut(s) 424
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 411
BciT130I CCWGG 1 cut(s) 138
BciVI GTATCC 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 223
BfuI GTATCC 1 cut(s) 134
BisI GCNGC 1 cut(s) 425
BlsI GCNGC 1 cut(s) 426
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 136
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 3 cut(s) 233, 412, 431
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 3 cut(s) 233, 412, 431
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 1 cut(s) 411
BseYI CCCAGC 1 cut(s) 282
BshNI GGYRCC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 204, 288
BsmFI GGGAC 2 cut(s) 204, 288
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 1 cut(s) 240
BspACI CCGC 2 cut(s) 247, 322
BspCNI CTCAG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 391
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 3 cut(s) 233, 412, 431
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 2 cut(s) 94, 182
BstF5I GGATG 1 cut(s) 132
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 411
BsuI GTATCC 1 cut(s) 134
BtsCI GGATG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 312
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 43
CviAII CATG 4 cut(s) 62, 76, 215, 421
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 2 cut(s) 94, 182
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 136
FaeI CATG 4 cut(s) 65, 79, 218, 424
FaqI GGGAC 2 cut(s) 204, 288
FatI CATG 4 cut(s) 61, 75, 214, 420
FauI CCCGC 1 cut(s) 254
FauNDI CATATG 1 cut(s) 197
Fnu4HI GCNGC 1 cut(s) 425
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 1 cut(s) 425
GluI GCNGC 1 cut(s) 425
GsaI CCCAGC 1 cut(s) 286
HapII CCGG 1 cut(s) 90
Hin1II CATG 4 cut(s) 65, 79, 218, 424
HinfI GANTC 2 cut(s) 192, 206
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 336, 353
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 2 cut(s) 210, 446
Hpy8I GTNNAC 2 cut(s) 336, 353
HpyAV CCTTC 1 cut(s) 397
HpyCH4V TGCA 1 cut(s) 82
HpyF3I CTNAG 2 cut(s) 94, 182
Hsp92II CATG 4 cut(s) 65, 79, 218, 424
Kzo9I GATC 1 cut(s) 240
Lsp1109I GCAGC 1 cut(s) 411
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 3 cut(s) 25, 222, 328
MlyI GAGTC 2 cut(s) 186, 215
MnlI CCTC 3 cut(s) 169, 248, 378
MseI TTAA 2 cut(s) 221, 327
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 197
NdeII GATC 1 cut(s) 240
NlaIII CATG 4 cut(s) 65, 79, 218, 424
NlaIV GGNNCC 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 345
PkrI GCNGC 1 cut(s) 426
PleI GAGTC 2 cut(s) 186, 214
PpsI GAGTC 2 cut(s) 186, 214
PpuMI RGGWCCY 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 391
PspPI GGNCC 1 cut(s) 257
PspPPI RGGWCCY 1 cut(s) 257
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 221, 327
SatI GCNGC 1 cut(s) 425
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 2 cut(s) 186, 215
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 394
SetI ASST 7 cut(s) 41, 172, 180, 237, 262, 312, 408
SinI GGWCC 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 3 cut(s) 25, 222, 328
SsiI CCGC 2 cut(s) 247, 322
StyD4I CCNGG 1 cut(s) 136
TaqI TCGA 2 cut(s) 53, 253
TasI AATT 3 cut(s) 25, 222, 328
Tru1I TTAA 2 cut(s) 221, 327
Tru9I TTAA 2 cut(s) 221, 327
TseI GCWGC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 406, 416
VpaK11BI GGWCC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.