Transposase family tnp2
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1479 |
| Acc65I | GGTACC | 1 cut(s) | 1460 |
| AccB1I | GGYRCC | 1 cut(s) | 1460 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 80 |
| AccI | GTMKAC | 1 cut(s) | 1409 |
| AciI | CCGC | 2 cut(s) | 388, 740 |
| AclWI | GGATC | 1 cut(s) | 1494 |
| AcsI | RAATTY | 1 cut(s) | 652 |
| AfaI | GTAC | 4 cut(s) | 52, 828, 1376, 1462 |
| AfeI | AGCGCT | 1 cut(s) | 1210 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 29, 80, 317, 745, 1002 |
| AflII | CTTAAG | 1 cut(s) | 830 |
| AflIII | ACRYGT | 2 cut(s) | 125, 507 |
| AgsI | TTSAA | 8 cut(s) | 260, 268, 416, 628, 808, 1151, 1181, 1559 |
| AhlI | ACTAGT | 1 cut(s) | 553 |
| AjnI | CCWGG | 3 cut(s) | 310, 1237, 1356 |
| AluBI | AGCT | 9 cut(s) | 602, 793, 850, 908, 947, 965, 1018, 1250, 1402 |
| AluI | AGCT | 9 cut(s) | 602, 793, 850, 908, 947, 965, 1018, 1250, 1402 |
| Alw26I | GTCTC | 3 cut(s) | 205, 841, 1344 |
| AlwI | GGATC | 1 cut(s) | 1494 |
| Aor51HI | AGCGCT | 1 cut(s) | 1210 |
| AoxI | GGCC | 1 cut(s) | 530 |
| ApeKI | GCWGC | 1 cut(s) | 1250 |
| ApoI | RAATTY | 1 cut(s) | 652 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 422, 454 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 255 |
| Asp718I | GGTACC | 1 cut(s) | 1460 |
| AspLEI | GCGC | 2 cut(s) | 821, 1211 |
| AspS9I | GGNCC | 1 cut(s) | 968 |
| AsuHPI | GGTGA | 3 cut(s) | 679, 760, 920 |
| AsuNHI | GCTAGC | 1 cut(s) | 1549 |
| AvaII | GGWCC | 1 cut(s) | 968 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1444, 1477 |
| BanI | GGYRCC | 1 cut(s) | 1460 |
| BbsI | GAAGAC | 1 cut(s) | 232 |
| BbvI | GCAGC | 1 cut(s) | 1237 |
| BccI | CCATC | 5 cut(s) | 238, 455, 539, 632, 1004 |
| BceAI | ACGGC | 1 cut(s) | 349 |
| BciT130I | CCWGG | 3 cut(s) | 312, 1239, 1358 |
| BciVI | GTATCC | 2 cut(s) | 1002, 1126 |
| BcoDI | GTCTC | 3 cut(s) | 205, 841, 1344 |
| BcuI | ACTAGT | 1 cut(s) | 553 |
| BfaI | CTAG | 6 cut(s) | 65, 534, 554, 1173, 1550, 1629 |
| BfoI | RGCGCY | 1 cut(s) | 1212 |
| BfrI | CTTAAG | 1 cut(s) | 830 |
| BfuI | GTATCC | 2 cut(s) | 1002, 1126 |
| BisI | GCNGC | 2 cut(s) | 389, 1251 |
| BlpI | GCTNAGC | 1 cut(s) | 1122 |
| BlsI | GCNGC | 2 cut(s) | 390, 1252 |
| BmcAI | AGTACT | 1 cut(s) | 828 |
| Bme1390I | CCNGG | 3 cut(s) | 312, 1239, 1358 |
| Bme18I | GGWCC | 1 cut(s) | 968 |
| BmgT120I | GGNCC | 1 cut(s) | 968 |
| BmiI | GGNNCC | 3 cut(s) | 633, 1462, 1665 |
| BmrFI | CCNGG | 3 cut(s) | 312, 1239, 1358 |
| BmsI | GCATC | 1 cut(s) | 1206 |
| BmtI | GCTAGC | 1 cut(s) | 1553 |
| BpiI | GAAGAC | 1 cut(s) | 232 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 202, 234 |
| BpmI | CTGGAG | 2 cut(s) | 696, 888 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1122 |
| BpuEI | CTTGAG | 2 cut(s) | 1518, 1551 |
| BsaBI | GATNNNNATC | 1 cut(s) | 151 |
| BsaI | GGTCTC | 1 cut(s) | 841 |
| BsaJI | CCNNGG | 3 cut(s) | 311, 1276, 1370 |
| BsaWI | WCCGGW | 1 cut(s) | 659 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 228, 258, 1326, 1356 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 29, 80, 317, 745, 1002 |
| Bse1I | ACTGG | 3 cut(s) | 338, 858, 1462 |
| Bse8I | GATNNNNATC | 1 cut(s) | 151 |
| BseBI | CCWGG | 3 cut(s) | 312, 1239, 1358 |
| BseDI | CCNNGG | 3 cut(s) | 311, 1276, 1370 |
| BseGI | GGATG | 5 cut(s) | 406, 550, 993, 1272, 1366 |
| BseJI | GATNNNNATC | 1 cut(s) | 151 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 29, 80, 317, 745, 1002 |
| BseMII | CTCAG | 2 cut(s) | 1113, 1226 |
| BseNI | ACTGG | 3 cut(s) | 338, 858, 1462 |
| BseRI | GAGGAG | 2 cut(s) | 1100, 1673 |
| BseXI | GCAGC | 1 cut(s) | 1237 |
| BseYI | CCCAGC | 1 cut(s) | 565 |
| BshFI | GGCC | 1 cut(s) | 532 |
| BshNI | GGYRCC | 1 cut(s) | 1460 |
| BsiSI | CCGG | 1 cut(s) | 660 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 29, 80, 317, 745, 1002 |
| BsmAI | GTCTC | 3 cut(s) | 205, 841, 1344 |
| BsnI | GGCC | 1 cut(s) | 532 |
| Bso31I | GGTCTC | 1 cut(s) | 841 |
| Bsp143I | GATC | 3 cut(s) | 160, 496, 1486 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1122 |
| Bsp19I | CCATGG | 1 cut(s) | 1370 |
| BspACI | CCGC | 2 cut(s) | 388, 740 |
| BspANI | GGCC | 1 cut(s) | 532 |
| BspCNI | CTCAG | 2 cut(s) | 1114, 1225 |
| BspHI | TCATGA | 2 cut(s) | 163, 706 |
| BspLI | GGNNCC | 3 cut(s) | 633, 1462, 1665 |
| BspOI | GCTAGC | 1 cut(s) | 1553 |
| BspPI | GGATC | 1 cut(s) | 1494 |
| BspT107I | GGYRCC | 1 cut(s) | 1460 |
| BspTI | CTTAAG | 1 cut(s) | 830 |
| BspTNI | GGTCTC | 1 cut(s) | 841 |
| BsrI | ACTGG | 3 cut(s) | 338, 858, 1462 |
| BssECI | CCNNGG | 3 cut(s) | 311, 1276, 1370 |
| BssMI | GATC | 3 cut(s) | 160, 496, 1486 |
| BssNAI | GTATAC | 1 cut(s) | 1410 |
| BssT1I | CCWWGG | 1 cut(s) | 1370 |
| Bst1107I | GTATAC | 1 cut(s) | 1410 |
| Bst2UI | CCWGG | 3 cut(s) | 312, 1239, 1358 |
| Bst4CI | ACNGT | 2 cut(s) | 381, 1331 |
| Bst6I | CTCTTC | 1 cut(s) | 1107 |
| BstAFI | CTTAAG | 1 cut(s) | 830 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 575 |
| BstC8I | GCNNGC | 2 cut(s) | 1248, 1551 |
| BstDEI | CTNAG | 5 cut(s) | 724, 943, 1122, 1212, 1314 |
| BstDSI | CCRYGG | 2 cut(s) | 1276, 1370 |
| BstF5I | GGATG | 5 cut(s) | 406, 550, 993, 1272, 1366 |
| BstH2I | RGCGCY | 1 cut(s) | 1212 |
| BstHHI | GCGC | 2 cut(s) | 821, 1211 |
| BstKTI | GATC | 3 cut(s) | 163, 499, 1489 |
| BstMAI | GTCTC | 3 cut(s) | 205, 841, 1344 |
| BstMBI | GATC | 3 cut(s) | 160, 496, 1486 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 441, 575, 953, 962, 1546, 1559 |
| BstNI | CCWGG | 3 cut(s) | 312, 1239, 1358 |
| BstNSI | RCATGY | 6 cut(s) | 72, 129, 218, 511, 1130, 1706 |
| BstSCI | CCNGG | 3 cut(s) | 310, 1237, 1356 |
| BstV1I | GCAGC | 1 cut(s) | 1237 |
| BstV2I | GAAGAC | 1 cut(s) | 232 |
| BstZ17I | GTATAC | 1 cut(s) | 1410 |
| BsuI | GTATCC | 2 cut(s) | 1002, 1126 |
| BsuRI | GGCC | 1 cut(s) | 532 |
| BtgI | CCRYGG | 2 cut(s) | 1276, 1370 |
| BtsCI | GGATG | 5 cut(s) | 406, 550, 993, 1272, 1366 |
| BtsIMutI | CAGTG | 3 cut(s) | 377, 1336, 1455 |
| Cac8I | GCNNGC | 2 cut(s) | 1248, 1551 |
| CciI | TCATGA | 2 cut(s) | 163, 706 |
| CfoI | GCGC | 2 cut(s) | 821, 1211 |
| Cfr13I | GGNCC | 1 cut(s) | 968 |
| Csp6I | GTAC | 4 cut(s) | 51, 827, 1375, 1461 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 965, 1000 |
| CviQI | GTAC | 4 cut(s) | 51, 827, 1375, 1461 |
| DdeI | CTNAG | 5 cut(s) | 724, 943, 1122, 1212, 1314 |
| DpnI | GATC | 3 cut(s) | 162, 498, 1488 |
| DpnII | GATC | 3 cut(s) | 160, 496, 1486 |
| DraI | TTTAAA | 1 cut(s) | 483 |
| Eam1104I | CTCTTC | 1 cut(s) | 1107 |
| EarI | CTCTTC | 1 cut(s) | 1107 |
| Eco130I | CCWWGG | 1 cut(s) | 1370 |
| Eco31I | GGTCTC | 1 cut(s) | 841 |
| Eco32I | GATATC | 1 cut(s) | 595 |
| Eco47I | GGWCC | 1 cut(s) | 968 |
| Eco47III | AGCGCT | 1 cut(s) | 1210 |
| EcoRII | CCWGG | 3 cut(s) | 310, 1237, 1356 |
| EcoRV | GATATC | 1 cut(s) | 595 |
| EcoT14I | CCWWGG | 1 cut(s) | 1370 |
| ErhI | CCWWGG | 1 cut(s) | 1370 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1509, 1541 |
| FauI | CCCGC | 1 cut(s) | 747 |
| FauNDI | CATATG | 2 cut(s) | 28, 280 |
| FblI | GTMKAC | 1 cut(s) | 1409 |
| Fnu4HI | GCNGC | 2 cut(s) | 389, 1251 |
| FokI | GGATG | 5 cut(s) | 413, 557, 1000, 1259, 1373 |
| Fsp4HI | GCNGC | 2 cut(s) | 389, 1251 |
| FspBI | CTAG | 6 cut(s) | 65, 534, 554, 1173, 1550, 1629 |
| GlaI | GCGC | 2 cut(s) | 820, 1210 |
| GluI | GCNGC | 2 cut(s) | 389, 1251 |
| GsaI | CCCAGC | 1 cut(s) | 569 |
| GsuI | CTGGAG | 2 cut(s) | 696, 888 |
| HaeII | RGCGCY | 1 cut(s) | 1212 |
| HaeIII | GGCC | 1 cut(s) | 532 |
| HapII | CCGG | 1 cut(s) | 660 |
| HhaI | GCGC | 2 cut(s) | 821, 1211 |
| Hin6I | GCGC | 2 cut(s) | 819, 1209 |
| HinP1I | GCGC | 2 cut(s) | 819, 1209 |
| HincII | GTYRAC | 1 cut(s) | 1510 |
| HindII | GTYRAC | 1 cut(s) | 1510 |
| HinfI | GANTC | 3 cut(s) | 476, 1310, 1587 |
| HpaI | GTTAAC | 1 cut(s) | 1510 |
| HpaII | CCGG | 1 cut(s) | 660 |
| HphI | GGTGA | 3 cut(s) | 679, 760, 920 |
| Hpy166II | GTNNAC | 5 cut(s) | 6, 551, 928, 1410, 1510 |
| Hpy188I | TCNGA | 4 cut(s) | 160, 841, 1215, 1689 |
| Hpy188III | TCNNGA | 8 cut(s) | 164, 234, 494, 635, 707, 1000, 1535, 1673 |
| Hpy8I | GTNNAC | 5 cut(s) | 6, 551, 928, 1410, 1510 |
| Hpy99I | CGWCG | 1 cut(s) | 147 |
| HpyAV | CCTTC | 4 cut(s) | 262, 283, 622, 1061 |
| HpyCH4III | ACNGT | 2 cut(s) | 381, 1331 |
| HpyCH4IV | ACGT | 1 cut(s) | 1136 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 441, 575, 953, 962, 1546, 1559 |
| HpyF3I | CTNAG | 5 cut(s) | 724, 943, 1122, 1212, 1314 |
| HpySE526I | ACGT | 1 cut(s) | 1136 |
| HspAI | GCGC | 2 cut(s) | 819, 1209 |
| KpnI | GGTACC | 1 cut(s) | 1464 |
| KspAI | GTTAAC | 1 cut(s) | 1510 |
| Kzo9I | GATC | 3 cut(s) | 160, 496, 1486 |
| LmnI | GCTCC | 4 cut(s) | 223, 1087, 1300, 1605 |
| Lsp1109I | GCAGC | 1 cut(s) | 1237 |
| LweI | GCATC | 1 cut(s) | 1206 |
| MaeI | CTAG | 6 cut(s) | 65, 534, 554, 1173, 1550, 1629 |
| MaeII | ACGT | 1 cut(s) | 1136 |
| MaeIII | GTNAC | 2 cut(s) | 615, 1265 |
| MalI | GATC | 3 cut(s) | 162, 498, 1488 |
| MboI | GATC | 3 cut(s) | 160, 496, 1486 |
| MlyI | GAGTC | 1 cut(s) | 1319 |
| MmeI | TCCRAC | 2 cut(s) | 26, 867 |
| MnlI | CCTC | 9 cut(s) | 532, 671, 772, 912, 996, 1078, 1576, 1651, 1654 |
| MroXI | GAANNNNTTC | 1 cut(s) | 255 |
| MseI | TTAA | 9 cut(s) | 482, 501, 831, 1076, 1344, 1437, 1502, 1509, 1527 |
| MslI | CAYNNNNRTG | 3 cut(s) | 73, 449, 1701 |
| MspCI | CTTAAG | 1 cut(s) | 830 |
| MspI | CCGG | 1 cut(s) | 660 |
| MspR9I | CCNGG | 3 cut(s) | 312, 1239, 1358 |
| MvaI | CCWGG | 3 cut(s) | 312, 1239, 1358 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 441, 575, 953, 962, 1546, 1559 |
| NcoI | CCATGG | 1 cut(s) | 1370 |
| NdeI | CATATG | 2 cut(s) | 28, 280 |
| NdeII | GATC | 3 cut(s) | 160, 496, 1486 |
| NheI | GCTAGC | 1 cut(s) | 1549 |
| NlaIV | GGNNCC | 3 cut(s) | 633, 1462, 1665 |
| NspI | RCATGY | 6 cut(s) | 72, 129, 218, 511, 1130, 1706 |
| PagI | TCATGA | 2 cut(s) | 163, 706 |
| PciI | ACATGT | 2 cut(s) | 125, 507 |
| PdmI | GAANNNNTTC | 1 cut(s) | 255 |
| PfeI | GAWTC | 2 cut(s) | 476, 1587 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 80 |
| PkrI | GCNGC | 2 cut(s) | 390, 1252 |
| PleI | GAGTC | 1 cut(s) | 1318 |
| PpsI | GAGTC | 1 cut(s) | 1318 |
| PscI | ACATGT | 2 cut(s) | 125, 507 |
| PsiI | TTATAA | 1 cut(s) | 1479 |
| Psp6I | CCWGG | 3 cut(s) | 310, 1237, 1356 |
| PspFI | CCCAGC | 1 cut(s) | 565 |
| PspGI | CCWGG | 3 cut(s) | 310, 1237, 1356 |
| PspN4I | GGNNCC | 3 cut(s) | 633, 1462, 1665 |
| PspPI | GGNCC | 1 cut(s) | 968 |
| RsaI | GTAC | 4 cut(s) | 52, 828, 1376, 1462 |
| RsaNI | GTAC | 4 cut(s) | 51, 827, 1375, 1461 |
| RseI | CAYNNNNRTG | 3 cut(s) | 73, 449, 1701 |
| SaqAI | TTAA | 9 cut(s) | 482, 501, 831, 1076, 1344, 1437, 1502, 1509, 1527 |
| SatI | GCNGC | 2 cut(s) | 389, 1251 |
| Sau3AI | GATC | 3 cut(s) | 160, 496, 1486 |
| Sau96I | GGNCC | 1 cut(s) | 968 |
| ScaI | AGTACT | 1 cut(s) | 828 |
| SchI | GAGTC | 1 cut(s) | 1319 |
| ScrFI | CCNGG | 3 cut(s) | 312, 1239, 1358 |
| SfaNI | GCATC | 1 cut(s) | 1206 |
| SinI | GGWCC | 1 cut(s) | 968 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 73, 449, 1701 |
| SmlI | CTYRAG | 3 cut(s) | 830, 1533, 1566 |
| SmoI | CTYRAG | 3 cut(s) | 830, 1533, 1566 |
| SpeI | ACTAGT | 1 cut(s) | 553 |
| SsiI | CCGC | 2 cut(s) | 388, 740 |
| SspI | AATATT | 2 cut(s) | 424, 897 |
| SspMI | CTAG | 6 cut(s) | 65, 534, 554, 1173, 1550, 1629 |
| StyD4I | CCNGG | 3 cut(s) | 310, 1237, 1356 |
| StyI | CCWWGG | 1 cut(s) | 1370 |
| TaaI | ACNGT | 2 cut(s) | 381, 1331 |
| TaiI | ACGT | 1 cut(s) | 1139 |
| TaqI | TCGA | 3 cut(s) | 145, 915, 999 |
| TatI | WGTACW | 1 cut(s) | 826 |
| TauI | GCSGC | 1 cut(s) | 391 |
| TfiI | GAWTC | 2 cut(s) | 476, 1587 |
| Tru1I | TTAA | 9 cut(s) | 482, 501, 831, 1076, 1344, 1437, 1502, 1509, 1527 |
| Tru9I | TTAA | 9 cut(s) | 482, 501, 831, 1076, 1344, 1437, 1502, 1509, 1527 |
| TscAI | CASTG | 3 cut(s) | 384, 1336, 1462 |
| TseI | GCWGC | 1 cut(s) | 1250 |
| TspDTI | ATGAA | 7 cut(s) | 180, 467, 713, 1022, 1280, 1656, 1731 |
| TspRI | CASTG | 3 cut(s) | 384, 1336, 1462 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 80 |
| Vha464I | CTTAAG | 1 cut(s) | 830 |
| VpaK11BI | GGWCC | 1 cut(s) | 968 |
| XapI | RAATTY | 1 cut(s) | 652 |
| XceI | RCATGY | 6 cut(s) | 72, 129, 218, 511, 1130, 1706 |
| XmiI | GTMKAC | 1 cut(s) | 1409 |
| XmnI | GAANNNNTTC | 1 cut(s) | 255 |
| XspI | CTAG | 6 cut(s) | 65, 534, 554, 1173, 1550, 1629 |
| ZrmI | AGTACT | 1 cut(s) | 828 |