Rorug04G0170400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000450
Physical Location & Seq
Forward (+)
29998117 .. 30015092
16976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0170400.1

Sequence Viewer

Length: 3441 bp
ATGGGTACTGAAGCATCATTAAGACTTGTTAGCAAACTTATAATTCTATGCCTCATCTTTACTCTTTGGCAGCTTGGCTCTGAATTCAAATATCAGAGTACAGCTCAACCAATGCCCGATGAAGAAGTGAGAGCTCTTAAAGAAATAGCATATAAGTTGGAGTTAGAAGGACGAATGTTCGATGAAGGCTCACTTTGCAATGGAAGCAGCGATGTTGGCGTCTACTGTGACTGCAGTTCCGGCACCATTTGCCACGTCAAATATATTAACCTACCTGCTCGCGGTTTGTCTGGAGTTATACCTGAAGAATTTGCCAATCTCACATATCTGCGAAGCTTGTATCTCAATAATAATCAATTGACTGGTCCCATACCTGCTAGCTTGGGGAAATTATCTTTGCTCTTCAGGCTGGATCTTTCGAATAATCAATTGACCGGGTCCATTCCAGCGAGTTTGGGAAATATGAAATTTCAAGCATTGTCCGAGGGATGGTCGCTTGATCGCCGCGTGGGTATTGAATTCACACTGGATCTTTCAGGCAATCAATTGAGTGGATCCATACCAGCCACTTTAGGGAATTTGACCCTCTCTTCGGTGATATCTGATCCAGAAGCCTACCGACTGATCGTCAAGCTTAAGCAATATGACAGTGCTTATGTAACCTCATCGTTCCACATGAGTCTTTCAAACAATCAACTGACTGGGCCTATACCAAAGAGCTTAGGGAATTTGACTTTCGCCGATACTATTGATCTGCATGGCAACTTTCTCAACGCCTCAATACCAGCATCTTTGGGTGCAGTGACTTTTCTTAGGTCTCTCACGCTTGGATCCAACAGTATCACTGGGACATTACCAAAAAGTTTTGCCAACCTTACAAGTCTGAAAACATTTTCTGTATCTGGTAACTATTTATCTGGCCCTTTACCCGACTTCATTGCCAACTGGACTTCAATCACTGAACTTTATCTGAACGGAAACAATTTCCAGGGGAAGATACCTGCCGGAATTTTTAATTTATCACATCTAGGAATTTTGGCAATAAGTGATGTGGAAGACTCTCATTTCCAGTTCCCACCATCTACACAAGTGACGAGCAGCTTTCGTATTTTGATACTGAGAAACTGCTCGATCACTGGTCAAATTCCTTATTACATTGGAAATATGTCATCATTATCGAACCTAGACCTGAGCTTCAACAGCTTAACGGGTAGTATCCCAGATTCTTTGAAAAACTTAGATTTAAGTTACTTGTCCTTTTCAAATAATATGCTTACTGGGGAAGTTCCTAATTGGATTCAAAATGCAGTTTGGAGTAAGATGGATCTTTCATACAATAATTTTTCAAAACTTACCTTTAAACCGTCAACCAAGTTAGACTTGAATTTGTTTTCCTGTTGCTGCAACTCCTCAACATGTCTACCGGATACGATGGACCCAACTCGAATAAAGGAGAAGTATTGTCCCCCGGAAAAACCTAATTACCATTCATTGTTTATAAATTGTGGTGGTGGGCAAACATTTGACATCGTTGATGGAAATGTTTATGATCAAGATAATGATACATCGCAGTTTTACCTGAGTCCAAAAGGAAATTGGGCTCGGAGTAGTGCTGGAAGCACCGTCGACCAAAACTATGCTTCCAATTCTAGTATATTCTTAAAAAGCGTGAGATGTGGGCTTTCCTCTGAAGCACATTTATACGATAGAGCTCGCACTTCTCCTGTCTCTCTAAAATATTACGGATTCTGTTTACGTGGAGGAAAATACAGTGTGACACTTCACTTTGCTGAAATCATTGATGAGGATAATGATCACAGAAGTACAAATAAACGCGTATTTGATGTATACATTCAGGGTGAGAGGAAACTAAAGGATTTCAACATTATGGACAAGGCAGGAGGTCCGAATAACGTATATCAAGAAAATTTCACGGCTGTTAATGTAAATGATAGTACATTAGAGATCCACTTCTACTCGGCTGGAGAAGGGCTTGATCAGGGACCTCTCATATCTGCTATATCCGTAACTCCAGAGTACAAGCTACACAAACAACTATCACCTCTACACATAGCGCTCATTACTGTGGCTTCAATCATAGTTTTTGTGTTGCTTTTATTGCTTTTTGCCTGGATGATGGGATGGCTGGGTACAGATCACTTACAAGAAATAGATATAGGTCTTGAAAAGCCCGTCACCCTCAAACAATTAAAAGATGCTACTCGGAATTTTAGCCAGAGGAACGAGATTGGTCAAGGGGGTTTTGGAACCGTTTACAAGGCTGAAGTGCAAGGGAAAATTGTAGCTGTGAAGAAACTTTCCTCTCATTCAGAGGAAAGGATCATTCAATTGAAAAATGAGTTTTATAACTTAAAATCAATGAGTCAAGAGAACCTTGTTCAGTTGCTGGACGTTTACAACACAAAAGGCCTGCATTTGCTCATCTATGAATATATGCAAAATAAGTCCCTTGCACACGCCTTATTTGACCCAAAGTCCAAACTGAAACTTGATTGGGAAGCTAGGTTTAACATTTGCTTGGGAATAGCTAGGGGATTGGTGTATCTACATGAGCATCCCAGGCTGAAGATGGTTCACAGGGACATTAAATCAGCTAATATTCTTCTCGATGGAAACCTCAAGGCTAAAATATCAGACTTTGGATTGGCAAGCCTTTACACCGAAGATGATCAATTCAAGTTCATCAAAGTAGAAATGCCAGAGGGATATATGGCACCTGAGTATGTTCGAGGAGTTGTGACATCCAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGAAAAATGCAGGACACAAGCGAGATAGCCAGGAAACTGAATTTCTTTTAGACACGGCTTGTGATTTACAGCGAAAAGGAAAGCTGGTGGACTTGGTTGATAAAACCTTGTCTAATAAGTATGATGCAAAACAAGCCATCATCATCTTGAATTTAGCAGTAAAGTGCATCAATATATCGCCAACTCTGAGGCCTACTATGTCTGAAGTTGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTTCCTCTGCTTTGATTGAGGACATCAACGACCCCCACGTGAAGGAGATCGCGGAGTTCGCGGTGTCGGAGTACAACAAGAAATCCGGGAAGAAGCTGGAGTTACAGAGCTTGGTGAAGGGTGAGACTCAAGTCGTCCCCGGCGAGAATTACAAGCTCGTCATCGCCGTCACGGATAACTCATCGGTGGCCAAGTACGAGGGCGTTGTGTATGAGAGGATTTGGGAACATACTAGGGAATTGCTCTCCTTCAATAGTCACATTGCTCAAGTTGATTCCTCTGTTTCTATGGAAGTAACCTCAAGAGTATCCACATCATCGAATTTGATCAAAGGGGAAGATGAAACAGAACACATTTCTGAGTGTACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

1146

Amino Acids

127.09

Weight (kDa)

5.9

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 85 - 144 1.1e-07 Leucine rich repeat
LRR_8 PF13855 271 - 329 6.1e-08 Leucine rich repeat
Malectin PF11721 497 - 674 7.2e-30 Malectin domain
Pkinase PF00069 745 - 1010 1.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 746 - 1012 4.4e-42 Protein tyrosine and serine/threonine kinase
SQAPI PF16845 1028 - 1107 1e-28 Aspartic acid proteinase inhibitor
Cystatin PF00031 1028 - 1093 7.9e-12 Cystatin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000450)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20080 FvH4_1g23272 FvH4_1g24190 FvH4_1g24191 FvH4_2g16992 FvH4_4g20600 FvH4_5g37430 FvH4_6g13850 FvH4_6g23331 FvH4_7g07732
prunus_persica Prupe.2G062800_v2.0.a1 Prupe.7G020400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0159341 RchiOBHm_Chr3g0461151 RchiOBHm_Chr3g0471341 RchiOBHm_Chr5g0079051
rosa_laevigata RLG00000013771 RLG00000016694 RLG00000019616 RLG00000020143 RLG00000022642 RLG00000030165
rosa_multiflora Rmu_sc0000058.1_g000010 Rmu_sc0000898.1_g000045 Rmu_sc0001200.1_g000007 Rmu_sc0001200.1_g000008 Rmu_sc0001323.1_g000001 Rmu_sc0002194.1_g000008 Rmu_sc0002206.1_g000017 Rmu_sc0002230.1_g000001 Rmu_sc0002280.1_g000001 Rmu_sc0003047.1_g000022 Rmu_sc0003623.1_g000007 Rmu_sc0003835.1_g000012
rosa_roxburghii Rroxscaffold_1G00019590 Rroxscaffold_1G00029010 Rroxscaffold_1G00029250 Rroxscaffold_1G00031650 Rroxscaffold_1G00031660 Rroxscaffold_1G00052850 Rroxscaffold_2G00144000 Rroxscaffold_3G00231810 Rroxscaffold_3G00245070 Rroxscaffold_3G00268690 Rroxscaffold_4G00312920 Rroxscaffold_5G00350060 Rroxscaffold_6G00412590 Rroxscaffold_6G00412600 Rroxscaffold_6G00420320 Rroxscaffold_7G00192970 Rroxscaffold_7G00197810 Rroxscaffold_7G00208250
rosa_rugosa Rorug01G0074000 Rorug01G0273600.1 Rorug01G0273700 Rorug03G0275700 Rorug04G0060600 Rorug04G0063100 Rorug04G0170400 Rorug05G0185600 Rorug05G0224200 Rorug05G0234600
rosa_samantha Rh1CG227300 Rh1DG133300 Rh1DG133400 Rh2AG004600 Rh2BG038900 Rh3BG045400 Rh3BG359100 Rh3BG359200 Rh3CG044000 Rh3CG355700 Rh4CG188000 Rh4CG217400 Rh4DG018500 Rh4DG092800 Rh4DG128600 Rh4DG141700 Rh5CG305800 Rh5DG275100 Rh5DG430600 Rh6DG316100
rosa_wichuraiana Rw0G005510 Rw0G010440 Rw0G010450 Rw1G018670 Rw3G028280 Rw4G010530 Rw4G010930 Rw4G012110 Rw4G012720 Rw5G007170 Rw7G023020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 41, 1499, 2367
Acc36I ACCTGC 3 cut(s) 283, 382, 1009
AccB1I GGYRCC 2 cut(s) 242, 2734
AccI GTMKAC 5 cut(s) 222, 1420, 1626, 1848, 2778
AccII CGCG 5 cut(s) 282, 507, 1836, 3119, 3128
AciI CCGC 4 cut(s) 282, 505, 3119, 3128
AcoI YGGCCR 1 cut(s) 3255
AcuI CTGAAG 7 cut(s) 30, 324, 388, 1710, 2304, 2606, 3042
AcvI CACGTG 1 cut(s) 3106
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 9 cut(s) 7, 100, 1825, 1957, 2039, 2152, 3140, 3263, 3433
AfeI AGCGCT 1 cut(s) 2076
AfiI CCNNNNNNNGG 5 cut(s) 281, 489, 573, 592, 3109
AflII CTTAAG 1 cut(s) 635
AflIII ACRYGT 2 cut(s) 1415, 1834
AhdI GACNNNNNGTC 2 cut(s) 626, 2494
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 4 cut(s) 987, 2129, 2579, 2847
Alw21I GWGCWC 2 cut(s) 136, 1714
Alw26I GTCTC 3 cut(s) 822, 1732, 3185
AlwNI CAGNNNCTG 1 cut(s) 2407
Aor51HI AGCGCT 1 cut(s) 2076
AoxI GGCC 5 cut(s) 704, 919, 2428, 3008, 3255
ApeKI GCWGC 4 cut(s) 70, 207, 1098, 1401
ArsI GACNNNNNNTTYG 4 cut(s) 574, 606, 2760, 2792
AspLEI GCGC 1 cut(s) 2077
AspS9I GGNCC 7 cut(s) 365, 438, 704, 920, 1435, 1904, 2003
AsuC2I CCSGG 4 cut(s) 436, 1469, 3154, 3207
AsuHPI GGTGA 6 cut(s) 607, 1871, 2052, 2188, 3193, 3200
AsuII TTCGAA 1 cut(s) 419
AsuNHI GCTAGC 1 cut(s) 377
AvaII GGWCC 5 cut(s) 365, 438, 1435, 1904, 2003
BalI TGGCCA 1 cut(s) 3257
BamHI GGATCC 2 cut(s) 554, 830
BanI GGYRCC 2 cut(s) 242, 2734
BanII GRGCYC 3 cut(s) 136, 1603, 1714
BbrPI CACGTG 1 cut(s) 3106
BbsI GAAGAC 1 cut(s) 1062
Bbv12I GWGCWC 2 cut(s) 136, 1714
BbvI GCAGC 4 cut(s) 82, 219, 1110, 1388
BceAI ACGGC 3 cut(s) 1950, 2889, 3218
BcgI CGANNNNNNTGC 2 cut(s) 920, 954
BciT130I CCWGG 4 cut(s) 989, 2131, 2581, 2849
BciVI GTATCC 3 cut(s) 1226, 1420, 3385
BclI TGATCA 5 cut(s) 1549, 1813, 1996, 2689, 3393
BcnI CCSGG 4 cut(s) 436, 1469, 3154, 3207
BcoDI GTCTC 3 cut(s) 822, 1732, 3185
BfaI CTAG 8 cut(s) 378, 1028, 1184, 1650, 2523, 2550, 3300, 3439
BfmI CTRYAG 2 cut(s) 232, 2779
BfoI RGCGCY 1 cut(s) 2078
BfrI CTTAAG 1 cut(s) 635
BfuAI ACCTGC 3 cut(s) 283, 382, 1009
BfuI GTATCC 3 cut(s) 1226, 1420, 3385
BisI GCNGC 5 cut(s) 71, 208, 505, 1099, 1402
BlsI GCNGC 5 cut(s) 72, 209, 506, 1100, 1403
Bme1390I CCNGG 8 cut(s) 436, 989, 1469, 2131, 2581, 2849, 3154, 3207
Bme18I GGWCC 5 cut(s) 365, 438, 1435, 1904, 2003
BmeRI GACNNNNNGTC 2 cut(s) 626, 2494
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 7 cut(s) 365, 438, 704, 920, 1435, 1904, 2003
BmiI GGNNCC 9 cut(s) 244, 367, 439, 556, 832, 1437, 2004, 2269, 2736
BmrFI CCNGG 8 cut(s) 436, 989, 1469, 2131, 2581, 2849, 3154, 3207
BmrI ACTGGG 3 cut(s) 711, 855, 1287
BmsI GCATC 6 cut(s) 23, 797, 2206, 2584, 2932, 2994
BmtI GCTAGC 1 cut(s) 381
BmuI ACTGGG 3 cut(s) 711, 855, 1287
BoxI GACNNNNGTC 1 cut(s) 3197
BpiI GAAGAC 1 cut(s) 1062
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpmI CTGGAG 4 cut(s) 312, 2004, 2016, 3185
Bpu10I CCTNAGC 2 cut(s) 721, 1190
Bpu14I TTCGAA 1 cut(s) 419
BpuEI CTTGAG 4 cut(s) 2624, 3180, 3318, 3352
BpuMI CCSGG 4 cut(s) 436, 1469, 3154, 3207
BsaAI YACGTR 2 cut(s) 1757, 3106
BsaBI GATNNNNATC 1 cut(s) 1812
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 822
BsaJI CCNNGG 5 cut(s) 483, 988, 1467, 2579, 3205
BsaWI WCCGGW 1 cut(s) 1423
Bsc4I CCNNNNNNNGG 5 cut(s) 281, 489, 573, 592, 3109
Bse1I ACTGG 8 cut(s) 367, 531, 706, 850, 950, 1069, 1141, 1282
Bse3DI GCAATG 3 cut(s) 205, 936, 3327
Bse8I GATNNNNATC 1 cut(s) 1812
BseBI CCWGG 4 cut(s) 989, 2131, 2581, 2849
BseDI CCNNGG 5 cut(s) 483, 988, 1467, 2579, 3205
BseGI GGATG 5 cut(s) 494, 2139, 2147, 2575, 2762
BseJI GATNNNNATC 1 cut(s) 1812
BseLI CCNNNNNNNGG 5 cut(s) 281, 489, 573, 592, 3109
BseMI GCAATG 3 cut(s) 205, 936, 3327
BseMII CTCAG 6 cut(s) 1109, 1181, 1571, 2730, 2996, 3417
BseNI ACTGG 8 cut(s) 367, 531, 706, 850, 950, 1069, 1141, 1282
BseRI GAGGAG 3 cut(s) 1399, 2766, 3074
BseXI GCAGC 4 cut(s) 82, 219, 1110, 1388
BseYI CCCAGC 1 cut(s) 2146
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 5 cut(s) 282, 507, 1836, 3119, 3128
BshFI GGCC 5 cut(s) 706, 921, 2430, 3010, 3257
BshNI GGYRCC 2 cut(s) 242, 2734
BsiHKAI GWGCWC 2 cut(s) 136, 1714
BsiSI CCGG 7 cut(s) 240, 435, 1005, 1424, 1469, 3153, 3207
BslFI GGGAC 7 cut(s) 351, 862, 1449, 2016, 2452, 2615, 3188
BslI CCNNNNNNNGG 5 cut(s) 281, 489, 573, 592, 3109
BsmAI GTCTC 3 cut(s) 822, 1732, 3185
BsmFI GGGAC 7 cut(s) 351, 862, 1449, 2016, 2452, 2615, 3188
BsnI GGCC 5 cut(s) 706, 921, 2430, 3010, 3257
Bso31I GGTCTC 1 cut(s) 822
Bsp119I TTCGAA 1 cut(s) 419
Bsp1286I GDGCHC 3 cut(s) 136, 1603, 1714
BspACI CCGC 4 cut(s) 282, 505, 3119, 3128
BspANI GGCC 5 cut(s) 706, 921, 2430, 3010, 3257
BspCNI CTCAG 6 cut(s) 1110, 1182, 1572, 2731, 2997, 3418
BspFNI CGCG 5 cut(s) 282, 507, 1836, 3119, 3128
BspLI GGNNCC 9 cut(s) 244, 367, 439, 556, 832, 1437, 2004, 2269, 2736
BspMAI CTGCAG 1 cut(s) 236
BspMI ACCTGC 3 cut(s) 283, 382, 1009
BspOI GCTAGC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 419
BspT107I GGYRCC 2 cut(s) 242, 2734
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 822
BsrDI GCAATG 3 cut(s) 205, 936, 3327
BsrI ACTGG 8 cut(s) 367, 531, 706, 850, 950, 1069, 1141, 1282
BssECI CCNNGG 5 cut(s) 483, 988, 1467, 2579, 3205
BssNAI GTATAC 1 cut(s) 1849
BssNI GRCGYC 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 1849
Bst2UI CCWGG 4 cut(s) 989, 2131, 2581, 2849
Bst6I CTCTTC 2 cut(s) 407, 595
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 1 cut(s) 249
BstBAI YACGTR 2 cut(s) 1757, 3106
BstBI TTCGAA 1 cut(s) 419
BstC8I GCNNGC 5 cut(s) 280, 379, 1714, 2432, 2671
BstDEI CTNAG 9 cut(s) 721, 812, 1118, 1190, 1237, 1580, 2739, 3005, 3426
BstF5I GGATG 5 cut(s) 494, 2139, 2147, 2575, 2762
BstFNI CGCG 5 cut(s) 282, 507, 1836, 3119, 3128
BstH2I RGCGCY 1 cut(s) 2078
BstHHI GCGC 1 cut(s) 2077
BstMAI GTCTC 3 cut(s) 822, 1732, 3185
BstNI CCWGG 4 cut(s) 989, 2131, 2581, 2849
BstNSI RCATGY 1 cut(s) 1419
BstPAI GACNNNNGTC 1 cut(s) 3197
BstSCI CCNGG 8 cut(s) 434, 987, 1467, 2129, 2579, 2847, 3152, 3205
BstSFI CTRYAG 2 cut(s) 232, 2779
BstUI CGCG 5 cut(s) 282, 507, 1836, 3119, 3128
BstV1I GCAGC 4 cut(s) 82, 219, 1110, 1388
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 6 cut(s) 412, 529, 554, 830, 1324, 1965
BstYI RGATCY 6 cut(s) 412, 529, 554, 830, 1324, 1965
BstZ17I GTATAC 1 cut(s) 1849
BsuI GTATCC 3 cut(s) 1226, 1420, 3385
BsuRI GGCC 5 cut(s) 706, 921, 2430, 3010, 3257
BtgZI GCGATG 3 cut(s) 225, 1551, 3214
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 5 cut(s) 494, 2139, 2147, 2575, 2762
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 7 cut(s) 524, 655, 807, 843, 957, 1134, 1777
BveI ACCTGC 3 cut(s) 283, 382, 1009
Cac8I GCNNGC 5 cut(s) 280, 379, 1714, 2432, 2671
CaiI CAGNNNCTG 1 cut(s) 2407
CfoI GCGC 1 cut(s) 2077
Cfr13I GGNCC 7 cut(s) 365, 438, 704, 920, 1435, 1904, 2003
CseI GACGC 1 cut(s) 208
Csp6I GTAC 9 cut(s) 6, 99, 1824, 1956, 2038, 2151, 3139, 3262, 3432
CviAII CATG 4 cut(s) 676, 758, 1416, 2570
CviQI GTAC 9 cut(s) 6, 99, 1824, 1956, 2038, 2151, 3139, 3262, 3432
DdeI CTNAG 9 cut(s) 721, 812, 1118, 1190, 1237, 1580, 2739, 3005, 3426
DraI TTTAAA 1 cut(s) 1360
DriI GACNNNNNGTC 2 cut(s) 626, 2494
EaeI YGGCCR 1 cut(s) 3255
Eam1104I CTCTTC 2 cut(s) 407, 595
Eam1105I GACNNNNNGTC 2 cut(s) 626, 2494
EarI CTCTTC 2 cut(s) 407, 595
Ecl136II GAGCTC 2 cut(s) 134, 1712
Eco147I AGGCCT 2 cut(s) 2430, 3010
Eco24I GRGCYC 3 cut(s) 136, 1603, 1714
Eco31I GGTCTC 1 cut(s) 822
Eco32I GATATC 1 cut(s) 600
Eco47I GGWCC 5 cut(s) 365, 438, 1435, 1904, 2003
Eco47III AGCGCT 1 cut(s) 2076
Eco53kI GAGCTC 2 cut(s) 134, 1712
Eco57I CTGAAG 7 cut(s) 30, 324, 388, 1710, 2304, 2606, 3042
Eco72I CACGTG 1 cut(s) 3106
EcoICRI GAGCTC 2 cut(s) 134, 1712
EcoO109I RGGNCCY 1 cut(s) 2003
EcoRI GAATTC 2 cut(s) 83, 518
EcoRII CCWGG 4 cut(s) 987, 2129, 2579, 2847
EcoRV GATATC 1 cut(s) 600
EcoT38I GRGCYC 3 cut(s) 136, 1603, 1714
FaeI CATG 4 cut(s) 679, 761, 1419, 2573
FalI AAGNNNNNCTT 6 cut(s) 177, 209, 1364, 1396, 2379, 2411
FaqI GGGAC 7 cut(s) 351, 862, 1449, 2016, 2452, 2615, 3188
FatI CATG 4 cut(s) 675, 757, 1415, 2569
FbaI TGATCA 5 cut(s) 1549, 1813, 1996, 2689, 3393
FblI GTMKAC 5 cut(s) 222, 1420, 1626, 1848, 2778
Fnu4HI GCNGC 5 cut(s) 71, 208, 505, 1099, 1402
FokI GGATG 5 cut(s) 501, 2146, 2154, 2562, 2749
FriOI GRGCYC 3 cut(s) 136, 1603, 1714
Fsp4HI GCNGC 5 cut(s) 71, 208, 505, 1099, 1402
FspBI CTAG 8 cut(s) 378, 1028, 1184, 1650, 2523, 2550, 3300, 3439
GlaI GCGC 1 cut(s) 2076
GluI GCNGC 5 cut(s) 71, 208, 505, 1099, 1402
GsaI CCCAGC 1 cut(s) 2150
GsuI CTGGAG 4 cut(s) 312, 2004, 2016, 3185
HaeII RGCGCY 1 cut(s) 2078
HaeIII GGCC 5 cut(s) 706, 921, 2430, 3010, 3257
HapII CCGG 7 cut(s) 240, 435, 1005, 1424, 1469, 3153, 3207
HgaI GACGC 1 cut(s) 208
HhaI GCGC 1 cut(s) 2077
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 679, 761, 1419, 2573
Hin6I GCGC 1 cut(s) 2075
HinP1I GCGC 1 cut(s) 2075
HincII GTYRAC 2 cut(s) 1368, 1627
HindII GTYRAC 2 cut(s) 1368, 1627
HindIII AAGCTT 2 cut(s) 334, 632
HinfI GANTC 9 cut(s) 679, 1058, 1223, 1297, 1582, 1746, 2383, 3193, 3341
HpaII CCGG 7 cut(s) 240, 435, 1005, 1424, 1469, 3153, 3207
HphI GGTGA 6 cut(s) 607, 1871, 2052, 2188, 3193, 3200
Hpy99I CGWCG 1 cut(s) 1628
HpyAV CCTTC 7 cut(s) 161, 179, 1982, 2811, 3103, 3178, 3325
HpyCH4IV ACGT 5 cut(s) 255, 1756, 1914, 2412, 3105
HpyF3I CTNAG 9 cut(s) 721, 812, 1118, 1190, 1237, 1580, 2739, 3005, 3426
HpySE526I ACGT 5 cut(s) 255, 1756, 1914, 2412, 3105
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 679, 761, 1419, 2573
HspAI GCGC 1 cut(s) 2075
Ksp22I TGATCA 5 cut(s) 1549, 1813, 1996, 2689, 3393
LguI GCTCTTC 1 cut(s) 407
Lsp1109I GCAGC 4 cut(s) 82, 219, 1110, 1388
LweI GCATC 6 cut(s) 23, 797, 2206, 2584, 2932, 2994
MaeI CTAG 8 cut(s) 378, 1028, 1184, 1650, 2523, 2550, 3300, 3439
MaeII ACGT 5 cut(s) 255, 1756, 1914, 2412, 3105
MfeI CAATTG 4 cut(s) 356, 428, 545, 2348
MflI RGATCY 6 cut(s) 412, 529, 554, 830, 1324, 1965
MhlI GDGCHC 3 cut(s) 136, 1603, 1714
MlsI TGGCCA 1 cut(s) 3257
MluI ACGCGT 1 cut(s) 1834
MluNI TGGCCA 1 cut(s) 3257
MlyI GAGTC 5 cut(s) 688, 1052, 1591, 2392, 3187
MmeI TCCRAC 3 cut(s) 138, 858, 3114
Mox20I TGGCCA 1 cut(s) 3257
MscI TGGCCA 1 cut(s) 3257
MslI CAYNNNNRTG 1 cut(s) 2084
Msp20I TGGCCA 1 cut(s) 3257
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 7 cut(s) 240, 435, 1005, 1424, 1469, 3153, 3207
MspR9I CCNGG 8 cut(s) 436, 989, 1469, 2131, 2581, 2849, 3154, 3207
MunI CAATTG 4 cut(s) 356, 428, 545, 2348
MvaI CCWGG 4 cut(s) 989, 2131, 2581, 2849
MvnI CGCG 5 cut(s) 282, 507, 1836, 3119, 3128
NciI CCSGG 4 cut(s) 436, 1469, 3154, 3207
NheI GCTAGC 1 cut(s) 377
NlaIII CATG 4 cut(s) 679, 761, 1419, 2573
NlaIV GGNNCC 9 cut(s) 244, 367, 439, 556, 832, 1437, 2004, 2269, 2736
NmeAIII GCCGAG 1 cut(s) 1958
NmuCI GTSAC 8 cut(s) 227, 802, 1090, 1774, 2194, 2758, 3235, 3323
NspI RCATGY 1 cut(s) 1419
NspV TTCGAA 1 cut(s) 419
PceI AGGCCT 2 cut(s) 2430, 3010
PciI ACATGT 1 cut(s) 1415
PciSI GCTCTTC 1 cut(s) 407
PcsI WCGNNNNNNNCGW 3 cut(s) 3102, 3207, 3231
PfeI GAWTC 4 cut(s) 1223, 1297, 1746, 3341
PflFI GACNNNGTC 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 3152
PkrI GCNGC 5 cut(s) 72, 209, 506, 1100, 1403
PleI GAGTC 5 cut(s) 687, 1052, 1590, 2391, 3187
PmaCI CACGTG 1 cut(s) 3106
PmlI CACGTG 1 cut(s) 3106
PpsI GAGTC 5 cut(s) 687, 1052, 1590, 2391, 3187
Ppu21I YACGTR 2 cut(s) 1757, 3106
PpuMI RGGWCCY 1 cut(s) 2003
PscI ACATGT 1 cut(s) 1415
PshAI GACNNNNGTC 1 cut(s) 3197
PsiI TTATAA 3 cut(s) 41, 1499, 2367
Psp124BI GAGCTC 2 cut(s) 136, 1714
Psp5II RGGWCCY 1 cut(s) 2003
Psp6I CCWGG 4 cut(s) 987, 2129, 2579, 2847
PspCI CACGTG 1 cut(s) 3106
PspFI CCCAGC 1 cut(s) 2146
PspGI CCWGG 4 cut(s) 987, 2129, 2579, 2847
PspN4I GGNNCC 9 cut(s) 244, 367, 439, 556, 832, 1437, 2004, 2269, 2736
PspPI GGNCC 7 cut(s) 365, 438, 704, 920, 1435, 1904, 2003
PspPPI RGGWCCY 1 cut(s) 2003
PstI CTGCAG 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 2407
PsuI RGATCY 6 cut(s) 412, 529, 554, 830, 1324, 1965
PsyI GACNNNGTC 1 cut(s) 436
RsaI GTAC 9 cut(s) 7, 100, 1825, 1957, 2039, 2152, 3140, 3263, 3433
RsaNI GTAC 9 cut(s) 6, 99, 1824, 1956, 2038, 2151, 3139, 3262, 3432
RseI CAYNNNNRTG 1 cut(s) 2084
SacI GAGCTC 2 cut(s) 136, 1714
SalI GTCGAC 1 cut(s) 1625
SapI GCTCTTC 1 cut(s) 407
SatI GCNGC 5 cut(s) 71, 208, 505, 1099, 1402
Sau96I GGNCC 7 cut(s) 365, 438, 704, 920, 1435, 1904, 2003
SchI GAGTC 5 cut(s) 688, 1052, 1591, 2392, 3187
ScrFI CCNGG 8 cut(s) 436, 989, 1469, 2131, 2581, 2849, 3154, 3207
SduI GDGCHC 3 cut(s) 136, 1603, 1714
SfaNI GCATC 6 cut(s) 23, 797, 2206, 2584, 2932, 2994
SfcI CTRYAG 2 cut(s) 232, 2779
SfuI TTCGAA 1 cut(s) 419
SinI GGWCC 5 cut(s) 365, 438, 1435, 1904, 2003
SmiMI CAYNNNNRTG 1 cut(s) 2084
SmlI CTYRAG 5 cut(s) 635, 2639, 3195, 3333, 3367
SmoI CTYRAG 5 cut(s) 635, 2639, 3195, 3333, 3367
SseBI AGGCCT 2 cut(s) 2430, 3010
SsiI CCGC 4 cut(s) 282, 505, 3119, 3128
SspI AATATT 2 cut(s) 1739, 2620
SspMI CTAG 8 cut(s) 378, 1028, 1184, 1650, 2523, 2550, 3300, 3439
SstI GAGCTC 2 cut(s) 136, 1714
StuI AGGCCT 2 cut(s) 2430, 3010
StyD4I CCNGG 8 cut(s) 434, 987, 1467, 2129, 2579, 2847, 3152, 3205
TaiI ACGT 5 cut(s) 258, 1759, 1917, 2415, 3108
TaqI TCGA 9 cut(s) 180, 419, 1130, 1178, 1444, 1626, 2628, 2749, 3386
TatI WGTACW 5 cut(s) 98, 1823, 1955, 2037, 3138
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 4 cut(s) 1223, 1297, 1746, 3341
TscAI CASTG 7 cut(s) 531, 655, 807, 850, 964, 1141, 1777
TseFI GTSAC 8 cut(s) 227, 802, 1090, 1774, 2194, 2758, 3235, 3323
TseI GCWGC 4 cut(s) 70, 207, 1098, 1401
Tsp45I GTSAC 8 cut(s) 227, 802, 1090, 1774, 2194, 2758, 3235, 3323
TspDTI ATGAA 9 cut(s) 135, 198, 479, 925, 1320, 1479, 2463, 2692, 3423
TspGWI ACGGA 4 cut(s) 990, 1758, 2014, 3254
TspRI CASTG 7 cut(s) 531, 655, 807, 850, 964, 1141, 1777
Tth111I GACNNNGTC 1 cut(s) 436
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 5 cut(s) 365, 438, 1435, 1904, 2003
XceI RCATGY 1 cut(s) 1419
XcmI CCANNNNNNNNNTGG 2 cut(s) 1593, 2587
XmiI GTMKAC 5 cut(s) 222, 1420, 1626, 1848, 2778
XspI CTAG 8 cut(s) 378, 1028, 1184, 1650, 2523, 2550, 3300, 3439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.