Transposase family tnp2
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1419 |
| AccB1I | GGYRCC | 2 cut(s) | 138, 568 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 397, 508 |
| AccI | GTMKAC | 3 cut(s) | 1108, 1254, 1349 |
| AciI | CCGC | 2 cut(s) | 740, 1332 |
| AclI | AACGTT | 1 cut(s) | 1575 |
| AclWI | GGATC | 2 cut(s) | 674, 1171 |
| AcsI | RAATTY | 1 cut(s) | 1150 |
| AcuI | CTGAAG | 1 cut(s) | 242 |
| AfaI | GTAC | 3 cut(s) | 52, 828, 1007 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 29, 317, 397, 508, 1002, 1657 |
| AflIII | ACRYGT | 1 cut(s) | 36 |
| AgsI | TTSAA | 8 cut(s) | 268, 483, 578, 628, 1001, 1468, 1523, 1583 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 96 |
| AhlI | ACTAGT | 1 cut(s) | 553 |
| AjiI | CACGTC | 1 cut(s) | 1309 |
| AjnI | CCWGG | 2 cut(s) | 1237, 1506 |
| AluBI | AGCT | 7 cut(s) | 506, 524, 587, 602, 793, 1250, 1400 |
| AluI | AGCT | 7 cut(s) | 506, 524, 587, 602, 793, 1250, 1400 |
| Alw21I | GWGCWC | 2 cut(s) | 220, 1213 |
| Alw26I | GTCTC | 2 cut(s) | 83, 724 |
| Alw44I | GTGCAC | 1 cut(s) | 216 |
| AlwI | GGATC | 2 cut(s) | 674, 1171 |
| AoxI | GGCC | 2 cut(s) | 530, 1227 |
| ApaLI | GTGCAC | 1 cut(s) | 216 |
| ApoI | RAATTY | 1 cut(s) | 1150 |
| AspLEI | GCGC | 1 cut(s) | 390 |
| AspS9I | GGNCC | 1 cut(s) | 1228 |
| AsuHPI | GGTGA | 2 cut(s) | 248, 1130 |
| BaeGI | GKGCMC | 1 cut(s) | 220 |
| BanI | GGYRCC | 2 cut(s) | 138, 568 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 240, 272 |
| BbsI | GAAGAC | 3 cut(s) | 232, 275, 380 |
| Bbv12I | GWGCWC | 2 cut(s) | 220, 1213 |
| BccI | CCATC | 5 cut(s) | 455, 539, 632, 978, 1190 |
| BciT130I | CCWGG | 2 cut(s) | 1239, 1508 |
| BciVI | GTATCC | 1 cut(s) | 1002 |
| BcoDI | GTCTC | 2 cut(s) | 83, 724 |
| BcuI | ACTAGT | 1 cut(s) | 553 |
| BfaI | CTAG | 3 cut(s) | 312, 534, 554 |
| BfuI | GTATCC | 1 cut(s) | 1002 |
| BmcAI | AGTACT | 1 cut(s) | 828 |
| Bme1390I | CCNGG | 2 cut(s) | 1239, 1508 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 96 |
| BmgBI | CACGTC | 1 cut(s) | 1309 |
| BmgT120I | GGNCC | 1 cut(s) | 1228 |
| BmiI | GGNNCC | 4 cut(s) | 140, 186, 570, 1289 |
| BmrFI | CCNGG | 2 cut(s) | 1239, 1508 |
| BmsI | GCATC | 2 cut(s) | 396, 1502 |
| BpiI | GAAGAC | 3 cut(s) | 232, 275, 380 |
| BsaBI | GATNNNNATC | 1 cut(s) | 410 |
| BsaJI | CCNNGG | 2 cut(s) | 1276, 1506 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 29, 317, 397, 508, 1002, 1657 |
| Bse1I | ACTGG | 2 cut(s) | 374, 533 |
| Bse8I | GATNNNNATC | 1 cut(s) | 410 |
| BseBI | CCWGG | 2 cut(s) | 1239, 1508 |
| BseDI | CCNNGG | 2 cut(s) | 1276, 1506 |
| BseGI | GGATG | 5 cut(s) | 406, 550, 993, 1138, 1493 |
| BseJI | GATNNNNATC | 1 cut(s) | 410 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 29, 317, 397, 508, 1002, 1657 |
| BseMII | CTCAG | 3 cut(s) | 697, 852, 1640 |
| BseNI | ACTGG | 2 cut(s) | 374, 533 |
| BseRI | GAGGAG | 1 cut(s) | 554 |
| BseSI | GKGCMC | 1 cut(s) | 220 |
| BsgI | GTGCAG | 1 cut(s) | 995 |
| BshFI | GGCC | 2 cut(s) | 532, 1229 |
| BshNI | GGYRCC | 2 cut(s) | 138, 568 |
| BsiHKAI | GWGCWC | 2 cut(s) | 220, 1213 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 29, 317, 397, 508, 1002, 1657 |
| BsmAI | GTCTC | 2 cut(s) | 83, 724 |
| BsnI | GGCC | 2 cut(s) | 532, 1229 |
| Bsp1286I | GDGCHC | 2 cut(s) | 220, 1213 |
| Bsp143I | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| Bsp19I | CCATGG | 1 cut(s) | 1276 |
| BspACI | CCGC | 2 cut(s) | 740, 1332 |
| BspANI | GGCC | 2 cut(s) | 532, 1229 |
| BspCNI | CTCAG | 3 cut(s) | 696, 851, 1639 |
| BspHI | TCATGA | 1 cut(s) | 163 |
| BspLI | GGNNCC | 4 cut(s) | 140, 186, 570, 1289 |
| BspPI | GGATC | 2 cut(s) | 674, 1171 |
| BspT107I | GGYRCC | 2 cut(s) | 138, 568 |
| BsrI | ACTGG | 2 cut(s) | 374, 533 |
| BssECI | CCNNGG | 2 cut(s) | 1276, 1506 |
| BssMI | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| BssNAI | GTATAC | 2 cut(s) | 1109, 1350 |
| BssT1I | CCWWGG | 1 cut(s) | 1276 |
| Bst1107I | GTATAC | 2 cut(s) | 1109, 1350 |
| Bst2UI | CCWGG | 2 cut(s) | 1239, 1508 |
| Bst4CI | ACNGT | 5 cut(s) | 143, 274, 1382, 1538, 1652 |
| Bst6I | CTCTTC | 2 cut(s) | 201, 695 |
| BstC8I | GCNNGC | 1 cut(s) | 1248 |
| BstDEI | CTNAG | 6 cut(s) | 683, 724, 838, 1170, 1442, 1626 |
| BstDSI | CCRYGG | 1 cut(s) | 1276 |
| BstF5I | GGATG | 5 cut(s) | 406, 550, 993, 1138, 1493 |
| BstHHI | GCGC | 1 cut(s) | 390 |
| BstKTI | GATC | 8 cut(s) | 163, 499, 565, 669, 1126, 1179, 1357, 1429 |
| BstMAI | GTCTC | 2 cut(s) | 83, 724 |
| BstMBI | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 748, 953, 1499 |
| BstNI | CCWGG | 2 cut(s) | 1239, 1508 |
| BstNSI | RCATGY | 2 cut(s) | 40, 72 |
| BstSCI | CCNGG | 2 cut(s) | 1237, 1506 |
| BstSLI | GKGCMC | 1 cut(s) | 220 |
| BstV2I | GAAGAC | 3 cut(s) | 232, 275, 380 |
| BstX2I | RGATCY | 1 cut(s) | 1176 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1238 |
| BstYI | RGATCY | 1 cut(s) | 1176 |
| BstZ17I | GTATAC | 2 cut(s) | 1109, 1350 |
| BsuI | GTATCC | 1 cut(s) | 1002 |
| BsuRI | GGCC | 2 cut(s) | 532, 1229 |
| BtgI | CCRYGG | 1 cut(s) | 1276 |
| BtrI | CACGTC | 1 cut(s) | 1309 |
| BtsCI | GGATG | 5 cut(s) | 406, 550, 993, 1138, 1493 |
| BtsIMutI | CAGTG | 2 cut(s) | 1378, 1543 |
| Cac8I | GCNNGC | 1 cut(s) | 1248 |
| CciI | TCATGA | 1 cut(s) | 163 |
| CfoI | GCGC | 1 cut(s) | 390 |
| Cfr13I | GGNCC | 1 cut(s) | 1228 |
| Csp6I | GTAC | 3 cut(s) | 51, 827, 1006 |
| CviQI | GTAC | 3 cut(s) | 51, 827, 1006 |
| DdeI | CTNAG | 6 cut(s) | 683, 724, 838, 1170, 1442, 1626 |
| DpnI | GATC | 8 cut(s) | 162, 498, 564, 668, 1125, 1178, 1356, 1428 |
| DpnII | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| DriI | GACNNNNNGTC | 1 cut(s) | 96 |
| Eam1104I | CTCTTC | 2 cut(s) | 201, 695 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 96 |
| EarI | CTCTTC | 2 cut(s) | 201, 695 |
| Eco130I | CCWWGG | 1 cut(s) | 1276 |
| Eco57I | CTGAAG | 1 cut(s) | 242 |
| EcoRII | CCWGG | 2 cut(s) | 1237, 1506 |
| EcoT14I | CCWWGG | 1 cut(s) | 1276 |
| EcoT22I | ATGCAT | 1 cut(s) | 411 |
| ErhI | CCWWGG | 1 cut(s) | 1276 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 508, 540 |
| FauNDI | CATATG | 2 cut(s) | 28, 1642 |
| FblI | GTMKAC | 3 cut(s) | 1108, 1254, 1349 |
| FokI | GGATG | 5 cut(s) | 413, 557, 1000, 1145, 1480 |
| FspBI | CTAG | 3 cut(s) | 312, 534, 554 |
| GlaI | GCGC | 1 cut(s) | 389 |
| HaeIII | GGCC | 2 cut(s) | 532, 1229 |
| HhaI | GCGC | 1 cut(s) | 390 |
| Hin6I | GCGC | 1 cut(s) | 388 |
| HinP1I | GCGC | 1 cut(s) | 388 |
| HincII | GTYRAC | 1 cut(s) | 1450 |
| HindII | GTYRAC | 1 cut(s) | 1450 |
| HindIII | AAGCTT | 1 cut(s) | 522 |
| HinfI | GANTC | 6 cut(s) | 325, 476, 681, 703, 1622, 1633 |
| HphI | GGTGA | 2 cut(s) | 248, 1130 |
| Hpy166II | GTNNAC | 7 cut(s) | 6, 53, 218, 1109, 1255, 1350, 1450 |
| Hpy188I | TCNGA | 2 cut(s) | 841, 1629 |
| Hpy188III | TCNNGA | 6 cut(s) | 164, 494, 635, 707, 854, 1613 |
| Hpy8I | GTNNAC | 7 cut(s) | 6, 53, 218, 1109, 1255, 1350, 1450 |
| Hpy99I | CGWCG | 1 cut(s) | 147 |
| HpyAV | CCTTC | 2 cut(s) | 1061, 1517 |
| HpyCH4III | ACNGT | 5 cut(s) | 143, 274, 1382, 1538, 1652 |
| HpyCH4IV | ACGT | 3 cut(s) | 435, 1308, 1575 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 748, 953, 1499 |
| HpyF3I | CTNAG | 6 cut(s) | 683, 724, 838, 1170, 1442, 1626 |
| HpySE526I | ACGT | 3 cut(s) | 435, 1308, 1575 |
| HspAI | GCGC | 1 cut(s) | 388 |
| Kzo9I | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| LweI | GCATC | 2 cut(s) | 396, 1502 |
| MaeI | CTAG | 3 cut(s) | 312, 534, 554 |
| MaeII | ACGT | 3 cut(s) | 435, 1308, 1575 |
| MaeIII | GTNAC | 8 cut(s) | 32, 615, 704, 756, 1265, 1309, 1634, 1652 |
| MalI | GATC | 8 cut(s) | 162, 498, 564, 668, 1125, 1178, 1356, 1428 |
| MboI | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| MfeI | CAATTG | 3 cut(s) | 331, 359, 1184 |
| MflI | RGATCY | 1 cut(s) | 1176 |
| MhlI | GDGCHC | 2 cut(s) | 220, 1213 |
| MlyI | GAGTC | 4 cut(s) | 319, 675, 712, 1642 |
| MmeI | TCCRAC | 4 cut(s) | 26, 644, 867, 1204 |
| MnlI | CCTC | 5 cut(s) | 129, 232, 532, 535, 912 |
| Mph1103I | ATGCAT | 1 cut(s) | 411 |
| MseI | TTAA | 1 cut(s) | 1019 |
| MslI | CAYNNNNRTG | 3 cut(s) | 73, 1119, 1641 |
| MspA1I | CMGCKG | 1 cut(s) | 587 |
| MspR9I | CCNGG | 2 cut(s) | 1239, 1508 |
| MunI | CAATTG | 3 cut(s) | 331, 359, 1184 |
| MvaI | CCWGG | 2 cut(s) | 1239, 1508 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 748, 953, 1499 |
| NcoI | CCATGG | 1 cut(s) | 1276 |
| NdeI | CATATG | 2 cut(s) | 28, 1642 |
| NdeII | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| NlaIV | GGNNCC | 4 cut(s) | 140, 186, 570, 1289 |
| NmuCI | GTSAC | 3 cut(s) | 704, 1309, 1634 |
| NsiI | ATGCAT | 1 cut(s) | 411 |
| NspI | RCATGY | 2 cut(s) | 40, 72 |
| PagI | TCATGA | 1 cut(s) | 163 |
| PciI | ACATGT | 1 cut(s) | 36 |
| PfeI | GAWTC | 2 cut(s) | 476, 1622 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 397, 508 |
| PfoI | TCCNGGA | 1 cut(s) | 1237 |
| PleI | GAGTC | 4 cut(s) | 319, 675, 711, 1641 |
| PpsI | GAGTC | 4 cut(s) | 319, 675, 711, 1641 |
| PscI | ACATGT | 1 cut(s) | 36 |
| PsiI | TTATAA | 1 cut(s) | 1419 |
| Psp1406I | AACGTT | 1 cut(s) | 1575 |
| Psp6I | CCWGG | 2 cut(s) | 1237, 1506 |
| PspGI | CCWGG | 2 cut(s) | 1237, 1506 |
| PspN4I | GGNNCC | 4 cut(s) | 140, 186, 570, 1289 |
| PspPI | GGNCC | 1 cut(s) | 1228 |
| PsuI | RGATCY | 1 cut(s) | 1176 |
| PvuII | CAGCTG | 1 cut(s) | 587 |
| RsaI | GTAC | 3 cut(s) | 52, 828, 1007 |
| RsaNI | GTAC | 3 cut(s) | 51, 827, 1006 |
| RseI | CAYNNNNRTG | 3 cut(s) | 73, 1119, 1641 |
| SaqAI | TTAA | 1 cut(s) | 1019 |
| Sau3AI | GATC | 8 cut(s) | 160, 496, 562, 666, 1123, 1176, 1354, 1426 |
| Sau96I | GGNCC | 1 cut(s) | 1228 |
| ScaI | AGTACT | 1 cut(s) | 828 |
| SchI | GAGTC | 4 cut(s) | 319, 675, 712, 1642 |
| ScrFI | CCNGG | 2 cut(s) | 1239, 1508 |
| SduI | GDGCHC | 2 cut(s) | 220, 1213 |
| SfaNI | GCATC | 2 cut(s) | 396, 1502 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 73, 1119, 1641 |
| SpeI | ACTAGT | 1 cut(s) | 553 |
| SsiI | CCGC | 2 cut(s) | 740, 1332 |
| SspI | AATATT | 1 cut(s) | 424 |
| SspMI | CTAG | 3 cut(s) | 312, 534, 554 |
| StyD4I | CCNGG | 2 cut(s) | 1237, 1506 |
| StyI | CCWWGG | 1 cut(s) | 1276 |
| TaaI | ACNGT | 5 cut(s) | 143, 274, 1382, 1538, 1652 |
| TaiI | ACGT | 3 cut(s) | 438, 1311, 1578 |
| TaqI | TCGA | 3 cut(s) | 145, 414, 429 |
| TatI | WGTACW | 2 cut(s) | 50, 826 |
| TfiI | GAWTC | 2 cut(s) | 476, 1622 |
| Tru1I | TTAA | 1 cut(s) | 1019 |
| Tru9I | TTAA | 1 cut(s) | 1019 |
| TscAI | CASTG | 2 cut(s) | 1385, 1543 |
| TseFI | GTSAC | 3 cut(s) | 704, 1309, 1634 |
| Tsp45I | GTSAC | 3 cut(s) | 704, 1309, 1634 |
| TspDTI | ATGAA | 8 cut(s) | 180, 267, 713, 917, 1022, 1611, 1617, 1659 |
| TspRI | CASTG | 2 cut(s) | 1385, 1543 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 397, 508 |
| VneI | GTGCAC | 1 cut(s) | 216 |
| XapI | RAATTY | 1 cut(s) | 1150 |
| XceI | RCATGY | 2 cut(s) | 40, 72 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 165 |
| XmiI | GTMKAC | 3 cut(s) | 1108, 1254, 1349 |
| XspI | CTAG | 3 cut(s) | 312, 534, 554 |
| ZrmI | AGTACT | 1 cut(s) | 828 |
| Zsp2I | ATGCAT | 1 cut(s) | 411 |