Transposase family tnp2
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1278 |
| Acc65I | GGTACC | 1 cut(s) | 138 |
| AccB1I | GGYRCC | 2 cut(s) | 138, 415 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 244, 355 |
| AccI | GTMKAC | 2 cut(s) | 904, 1208 |
| AclWI | GGATC | 4 cut(s) | 124, 404, 521, 967 |
| AcsI | RAATTY | 1 cut(s) | 947 |
| AfaI | GTAC | 4 cut(s) | 52, 140, 818, 1095 |
| AfeI | AGCGCT | 1 cut(s) | 1006 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 29, 244, 355, 1175, 1462 |
| AflIII | ACRYGT | 1 cut(s) | 36 |
| AgsI | TTSAA | 4 cut(s) | 425, 498, 1327, 1382 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 96 |
| AhlI | ACTAGT | 1 cut(s) | 400 |
| AjiI | CACGTC | 1 cut(s) | 1105 |
| AjnI | CCWGG | 1 cut(s) | 1033 |
| AluBI | AGCT | 8 cut(s) | 353, 371, 434, 449, 604, 1046, 1201, 1259 |
| AluI | AGCT | 8 cut(s) | 353, 371, 434, 449, 604, 1046, 1201, 1259 |
| Alw26I | GTCTC | 3 cut(s) | 83, 652, 1054 |
| AlwI | GGATC | 4 cut(s) | 124, 404, 521, 967 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 482 |
| Ama87I | CYCGRG | 1 cut(s) | 921 |
| Aor51HI | AGCGCT | 1 cut(s) | 1006 |
| AoxI | GGCC | 2 cut(s) | 779, 1023 |
| ApoI | RAATTY | 1 cut(s) | 947 |
| AseI | ATTAAT | 1 cut(s) | 1158 |
| Asp718I | GGTACC | 1 cut(s) | 138 |
| AspLEI | GCGC | 3 cut(s) | 85, 237, 1007 |
| AspS9I | GGNCC | 2 cut(s) | 779, 1024 |
| AsuHPI | GGTGA | 1 cut(s) | 926 |
| AvaI | CYCGRG | 1 cut(s) | 921 |
| BanI | GGYRCC | 2 cut(s) | 138, 415 |
| BauI | CACGAG | 1 cut(s) | 1435 |
| BbvCI | CCTCAGC | 1 cut(s) | 641 |
| BccI | CCATC | 5 cut(s) | 302, 386, 479, 789, 986 |
| BciT130I | CCWGG | 1 cut(s) | 1035 |
| BciVI | GTATCC | 2 cut(s) | 813, 1363 |
| BcoDI | GTCTC | 3 cut(s) | 83, 652, 1054 |
| BcuI | ACTAGT | 1 cut(s) | 400 |
| BfaI | CTAG | 3 cut(s) | 96, 381, 401 |
| BfoI | RGCGCY | 1 cut(s) | 1008 |
| BfuI | GTATCC | 2 cut(s) | 813, 1363 |
| Bme1390I | CCNGG | 1 cut(s) | 1035 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 96 |
| BmeT110I | CYCGRG | 1 cut(s) | 921 |
| BmgBI | CACGTC | 1 cut(s) | 1105 |
| BmgT120I | GGNCC | 2 cut(s) | 779, 1024 |
| BmiI | GGNNCC | 4 cut(s) | 140, 186, 417, 1180 |
| BmrFI | CCNGG | 1 cut(s) | 1035 |
| BmsI | GCATC | 2 cut(s) | 243, 907 |
| Bpu10I | CCTNAGC | 1 cut(s) | 641 |
| BsaBI | GATNNNNATC | 1 cut(s) | 257 |
| BsaI | GGTCTC | 1 cut(s) | 652 |
| BsaJI | CCNNGG | 2 cut(s) | 1072, 1169 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 29, 244, 355, 1175, 1462 |
| Bse1I | ACTGG | 1 cut(s) | 380 |
| Bse8I | GATNNNNATC | 1 cut(s) | 257 |
| BseBI | CCWGG | 1 cut(s) | 1035 |
| BseDI | CCNNGG | 2 cut(s) | 1072, 1169 |
| BseGI | GGATG | 4 cut(s) | 253, 397, 804, 934 |
| BseJI | GATNNNNATC | 1 cut(s) | 257 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 29, 244, 355, 1175, 1462 |
| BseMII | CTCAG | 2 cut(s) | 632, 1379 |
| BseNI | ACTGG | 1 cut(s) | 380 |
| BseRI | GAGGAG | 1 cut(s) | 401 |
| BsgI | GTGCAG | 1 cut(s) | 806 |
| BshFI | GGCC | 2 cut(s) | 781, 1025 |
| BshNI | GGYRCC | 2 cut(s) | 138, 415 |
| BsiHKCI | CYCGRG | 1 cut(s) | 921 |
| BsiSI | CCGG | 1 cut(s) | 413 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 29, 244, 355, 1175, 1462 |
| BsmAI | GTCTC | 3 cut(s) | 83, 652, 1054 |
| BsnI | GGCC | 2 cut(s) | 781, 1025 |
| Bso31I | GGTCTC | 1 cut(s) | 652 |
| BsoBI | CYCGRG | 1 cut(s) | 921 |
| Bsp1407I | TGTACA | 1 cut(s) | 1093 |
| Bsp143I | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| Bsp19I | CCATGG | 1 cut(s) | 1072 |
| BspANI | GGCC | 2 cut(s) | 781, 1025 |
| BspCNI | CTCAG | 2 cut(s) | 633, 1378 |
| BspHI | TCATGA | 2 cut(s) | 163, 553 |
| BspLI | GGNNCC | 4 cut(s) | 140, 186, 417, 1180 |
| BspPI | GGATC | 4 cut(s) | 124, 404, 521, 967 |
| BspT107I | GGYRCC | 2 cut(s) | 138, 415 |
| BspTNI | GGTCTC | 1 cut(s) | 652 |
| BsrGI | TGTACA | 1 cut(s) | 1093 |
| BsrI | ACTGG | 1 cut(s) | 380 |
| BssECI | CCNNGG | 2 cut(s) | 1072, 1169 |
| BssMI | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| BssNAI | GTATAC | 2 cut(s) | 905, 1209 |
| BssSI | CACGAG | 1 cut(s) | 1435 |
| BssT1I | CCWWGG | 2 cut(s) | 1072, 1169 |
| Bst1107I | GTATAC | 2 cut(s) | 905, 1209 |
| Bst2BI | CACGAG | 1 cut(s) | 1435 |
| Bst2UI | CCWGG | 1 cut(s) | 1035 |
| Bst4CI | ACNGT | 5 cut(s) | 143, 478, 1093, 1241, 1457 |
| Bst6I | CTCTTC | 2 cut(s) | 201, 542 |
| BstAUI | TGTACA | 1 cut(s) | 1093 |
| BstC8I | GCNNGC | 1 cut(s) | 1044 |
| BstDEI | CTNAG | 3 cut(s) | 641, 966, 1365 |
| BstDSI | CCRYGG | 1 cut(s) | 1072 |
| BstF5I | GGATG | 4 cut(s) | 253, 397, 804, 934 |
| BstH2I | RGCGCY | 1 cut(s) | 1008 |
| BstHHI | GCGC | 3 cut(s) | 85, 237, 1007 |
| BstKTI | GATC | 7 cut(s) | 119, 163, 346, 412, 516, 975, 1288 |
| BstMAI | GTCTC | 3 cut(s) | 83, 652, 1054 |
| BstMBI | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 764, 1345 |
| BstNI | CCWGG | 1 cut(s) | 1035 |
| BstNSI | RCATGY | 1 cut(s) | 40 |
| BstSCI | CCNGG | 1 cut(s) | 1033 |
| BstX2I | RGATCY | 1 cut(s) | 972 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1034 |
| BstYI | RGATCY | 1 cut(s) | 972 |
| BstZ17I | GTATAC | 2 cut(s) | 905, 1209 |
| BsuI | GTATCC | 2 cut(s) | 813, 1363 |
| BsuRI | GGCC | 2 cut(s) | 781, 1025 |
| BtgI | CCRYGG | 1 cut(s) | 1072 |
| BtrI | CACGTC | 1 cut(s) | 1105 |
| BtsCI | GGATG | 4 cut(s) | 253, 397, 804, 934 |
| BtsIMutI | CAGTG | 1 cut(s) | 1237 |
| Cac8I | GCNNGC | 1 cut(s) | 1044 |
| CaiI | CAGNNNCTG | 1 cut(s) | 482 |
| CciI | TCATGA | 2 cut(s) | 163, 553 |
| CfoI | GCGC | 3 cut(s) | 85, 237, 1007 |
| Cfr13I | GGNCC | 2 cut(s) | 779, 1024 |
| CseI | GACGC | 1 cut(s) | 537 |
| Csp6I | GTAC | 4 cut(s) | 51, 139, 817, 1094 |
| CviQI | GTAC | 4 cut(s) | 51, 139, 817, 1094 |
| DdeI | CTNAG | 3 cut(s) | 641, 966, 1365 |
| DpnI | GATC | 7 cut(s) | 118, 162, 345, 411, 515, 974, 1287 |
| DpnII | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| DraI | TTTAAA | 1 cut(s) | 330 |
| DriI | GACNNNNNGTC | 1 cut(s) | 96 |
| Eam1104I | CTCTTC | 2 cut(s) | 201, 542 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 96 |
| EarI | CTCTTC | 2 cut(s) | 201, 542 |
| Eco130I | CCWWGG | 2 cut(s) | 1072, 1169 |
| Eco31I | GGTCTC | 1 cut(s) | 652 |
| Eco47III | AGCGCT | 1 cut(s) | 1006 |
| Eco88I | CYCGRG | 1 cut(s) | 921 |
| EcoRII | CCWGG | 1 cut(s) | 1033 |
| EcoT14I | CCWWGG | 2 cut(s) | 1072, 1169 |
| EcoT22I | ATGCAT | 1 cut(s) | 258 |
| ErhI | CCWWGG | 2 cut(s) | 1072, 1169 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 355, 387, 882, 914 |
| FauNDI | CATATG | 2 cut(s) | 28, 1447 |
| FblI | GTMKAC | 2 cut(s) | 904, 1208 |
| FokI | GGATG | 4 cut(s) | 260, 404, 811, 941 |
| FspBI | CTAG | 3 cut(s) | 96, 381, 401 |
| GlaI | GCGC | 3 cut(s) | 84, 236, 1006 |
| HaeII | RGCGCY | 1 cut(s) | 1008 |
| HaeIII | GGCC | 2 cut(s) | 781, 1025 |
| HapII | CCGG | 1 cut(s) | 413 |
| HgaI | GACGC | 1 cut(s) | 537 |
| HhaI | GCGC | 3 cut(s) | 85, 237, 1007 |
| Hin6I | GCGC | 3 cut(s) | 83, 235, 1005 |
| HinP1I | GCGC | 3 cut(s) | 83, 235, 1005 |
| HincII | GTYRAC | 1 cut(s) | 1309 |
| HindII | GTYRAC | 1 cut(s) | 1309 |
| HindIII | AAGCTT | 1 cut(s) | 369 |
| HinfI | GANTC | 4 cut(s) | 323, 550, 1083, 1438 |
| HpaII | CCGG | 1 cut(s) | 413 |
| HphI | GGTGA | 1 cut(s) | 926 |
| Hpy166II | GTNNAC | 6 cut(s) | 6, 53, 176, 905, 1209, 1309 |
| Hpy188I | TCNGA | 1 cut(s) | 652 |
| Hpy188III | TCNNGA | 5 cut(s) | 164, 381, 482, 554, 665 |
| Hpy8I | GTNNAC | 6 cut(s) | 6, 53, 176, 905, 1209, 1309 |
| Hpy99I | CGWCG | 2 cut(s) | 147, 531 |
| HpyAV | CCTTC | 1 cut(s) | 857 |
| HpyCH4III | ACNGT | 5 cut(s) | 143, 478, 1093, 1241, 1457 |
| HpyCH4IV | ACGT | 3 cut(s) | 282, 1104, 1191 |
| HpyCH4V | TGCA | 9 cut(s) | 26, 218, 256, 693, 719, 787, 823, 937, 1451 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 764, 1345 |
| HpyF3I | CTNAG | 3 cut(s) | 641, 966, 1365 |
| HpySE526I | ACGT | 3 cut(s) | 282, 1104, 1191 |
| HspAI | GCGC | 3 cut(s) | 83, 235, 1005 |
| KpnI | GGTACC | 1 cut(s) | 142 |
| Kzo9I | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| LweI | GCATC | 2 cut(s) | 243, 907 |
| MaeI | CTAG | 3 cut(s) | 96, 381, 401 |
| MaeII | ACGT | 3 cut(s) | 282, 1104, 1191 |
| MaeIII | GTNAC | 8 cut(s) | 32, 462, 566, 1061, 1105, 1214, 1439, 1457 |
| MalI | GATC | 7 cut(s) | 118, 162, 345, 411, 515, 974, 1287 |
| MboI | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| MboII | GAAGA | 8 cut(s) | 218, 559, 890, 890, 893, 982, 1123, 1295 |
| MfeI | CAATTG | 1 cut(s) | 980 |
| MflI | RGATCY | 1 cut(s) | 972 |
| MlyI | GAGTC | 2 cut(s) | 559, 1447 |
| MmeI | TCCRAC | 4 cut(s) | 26, 491, 678, 1000 |
| MnlI | CCTC | 6 cut(s) | 129, 379, 382, 636, 723, 1456 |
| Mph1103I | ATGCAT | 1 cut(s) | 258 |
| MseI | TTAA | 5 cut(s) | 329, 810, 830, 1158, 1301 |
| MslI | CAYNNNNRTG | 5 cut(s) | 73, 296, 812, 915, 1446 |
| MspA1I | CMGCKG | 1 cut(s) | 434 |
| MspI | CCGG | 1 cut(s) | 413 |
| MspR9I | CCNGG | 1 cut(s) | 1035 |
| MunI | CAATTG | 1 cut(s) | 980 |
| MvaI | CCWGG | 1 cut(s) | 1035 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 764, 1345 |
| NcoI | CCATGG | 1 cut(s) | 1072 |
| NdeI | CATATG | 2 cut(s) | 28, 1447 |
| NdeII | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| NlaIV | GGNNCC | 4 cut(s) | 140, 186, 417, 1180 |
| NmuCI | GTSAC | 3 cut(s) | 1105, 1214, 1439 |
| NsiI | ATGCAT | 1 cut(s) | 258 |
| NspI | RCATGY | 1 cut(s) | 40 |
| PagI | TCATGA | 2 cut(s) | 163, 553 |
| PciI | ACATGT | 1 cut(s) | 36 |
| PfeI | GAWTC | 2 cut(s) | 323, 1083 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 244, 355 |
| PfoI | TCCNGGA | 1 cut(s) | 1033 |
| PleI | GAGTC | 2 cut(s) | 558, 1446 |
| PpsI | GAGTC | 2 cut(s) | 558, 1446 |
| PscI | ACATGT | 1 cut(s) | 36 |
| PshBI | ATTAAT | 1 cut(s) | 1158 |
| PsiI | TTATAA | 1 cut(s) | 1278 |
| Psp6I | CCWGG | 1 cut(s) | 1033 |
| PspGI | CCWGG | 1 cut(s) | 1033 |
| PspN4I | GGNNCC | 4 cut(s) | 140, 186, 417, 1180 |
| PspPI | GGNCC | 2 cut(s) | 779, 1024 |
| PstNI | CAGNNNCTG | 1 cut(s) | 482 |
| PsuI | RGATCY | 1 cut(s) | 972 |
| PvuII | CAGCTG | 1 cut(s) | 434 |
| RsaI | GTAC | 4 cut(s) | 52, 140, 818, 1095 |
| RsaNI | GTAC | 4 cut(s) | 51, 139, 817, 1094 |
| RseI | CAYNNNNRTG | 5 cut(s) | 73, 296, 812, 915, 1446 |
| SaqAI | TTAA | 5 cut(s) | 329, 810, 830, 1158, 1301 |
| Sau3AI | GATC | 7 cut(s) | 116, 160, 343, 409, 513, 972, 1285 |
| Sau96I | GGNCC | 2 cut(s) | 779, 1024 |
| SchI | GAGTC | 2 cut(s) | 559, 1447 |
| ScrFI | CCNGG | 1 cut(s) | 1035 |
| SfaNI | GCATC | 2 cut(s) | 243, 907 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 73, 296, 812, 915, 1446 |
| SpeI | ACTAGT | 1 cut(s) | 400 |
| SspI | AATATT | 2 cut(s) | 128, 271 |
| SspMI | CTAG | 3 cut(s) | 96, 381, 401 |
| StyD4I | CCNGG | 1 cut(s) | 1033 |
| StyI | CCWWGG | 2 cut(s) | 1072, 1169 |
| TaaI | ACNGT | 5 cut(s) | 143, 478, 1093, 1241, 1457 |
| TaiI | ACGT | 3 cut(s) | 285, 1107, 1194 |
| TaqI | TCGA | 4 cut(s) | 145, 261, 276, 1081 |
| TatI | WGTACW | 3 cut(s) | 50, 816, 1093 |
| TfiI | GAWTC | 2 cut(s) | 323, 1083 |
| Tru1I | TTAA | 5 cut(s) | 329, 810, 830, 1158, 1301 |
| Tru9I | TTAA | 5 cut(s) | 329, 810, 830, 1158, 1301 |
| TscAI | CASTG | 1 cut(s) | 1244 |
| TseFI | GTSAC | 3 cut(s) | 1105, 1214, 1439 |
| Tsp45I | GTSAC | 3 cut(s) | 1105, 1214, 1439 |
| TspDTI | ATGAA | 4 cut(s) | 180, 314, 560, 728 |
| TspRI | CASTG | 1 cut(s) | 1244 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 244, 355 |
| VspI | ATTAAT | 1 cut(s) | 1158 |
| XapI | RAATTY | 1 cut(s) | 947 |
| XbaI | TCTAGA | 1 cut(s) | 380 |
| XceI | RCATGY | 1 cut(s) | 40 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 165 |
| XmiI | GTMKAC | 2 cut(s) | 904, 1208 |
| XspI | CTAG | 3 cut(s) | 96, 381, 401 |
| Zsp2I | ATGCAT | 1 cut(s) | 258 |