Rorug02G0020600

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014822
Physical Location & Seq
Forward (+)
1657910 .. 1658293
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0020600.1

Sequence Viewer

Length: 384 bp
ATGGCAATGAAAATTGTAGCATTATCTGCACTGCTACTAGTGGCATTACTCACCACCTTGCTATCCCAACCAGGAATAGCCAATGCGCAGTCGCCACAGAGTCCTCCTTCGGCGGTTCCAGGCCTGCTTCCACCAGGAACTCCGAATGATGTCCTCAAGTGTTGGTCATCTCTTACCGGCATTAGTGGATGTGTTTTGGAAATCTTTCGATCTGTTTTCAGCTTGCAATTAGGCACCATTGGTGCCGACTGTTGCAAGGCCTTCCTTTCTATCGAGGAAAGCTGTTGGCCCAAAATGTTTCCTCTGACTCCTTTCTTCCCTCCCTTGCTCAAGAACATTTGTGAGCTCCAAGGAAGAGGTCCTGCGCCCCCAGCATTCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

127

Amino Acids

13.37

Weight (kDa)

7.55

Isoelectric Point (pI)

71.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 53 - 114 9e-17 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 87
AccB1I GGYRCC 2 cut(s) 233, 242
AciI CCGC 1 cut(s) 113
AhlI ACTAGT 1 cut(s) 37
AjnI CCWGG 3 cut(s) 70, 118, 133
AluBI AGCT 3 cut(s) 222, 282, 346
AluI AGCT 3 cut(s) 222, 282, 346
Alw21I GWGCWC 1 cut(s) 348
AoxI GGCC 3 cut(s) 121, 258, 287
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 2 cut(s) 88, 367
AspS9I GGNCC 2 cut(s) 288, 359
AsuHPI GGTGA 1 cut(s) 43
AvaII GGWCC 1 cut(s) 359
BanI GGYRCC 2 cut(s) 233, 242
BanII GRGCYC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 348
BciT130I CCWGG 3 cut(s) 72, 120, 135
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 1 cut(s) 38
Bme1390I CCNGG 3 cut(s) 72, 120, 135
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 2 cut(s) 288, 359
BmiI GGNNCC 3 cut(s) 117, 235, 244
BmrFI CCNGG 3 cut(s) 72, 120, 135
BpuEI CTTGAG 2 cut(s) 140, 314
BsaJI CCNNGG 1 cut(s) 349
Bse118I RCCGGY 1 cut(s) 176
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 3 cut(s) 72, 120, 135
BseDI CCNNGG 1 cut(s) 349
BseGI GGATG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 12
BseYI CCCAGC 1 cut(s) 370
BsgI GTGCAG 1 cut(s) 12
BshFI GGCC 3 cut(s) 123, 260, 289
BshNI GGYRCC 2 cut(s) 233, 242
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 1 cut(s) 177
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 3 cut(s) 123, 260, 289
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 1 cut(s) 113
BspANI GGCC 3 cut(s) 123, 260, 289
BspLI GGNNCC 3 cut(s) 117, 235, 244
BspT107I GGYRCC 2 cut(s) 233, 242
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 3 cut(s) 72, 120, 135
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 349
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 125, 224
BstF5I GGATG 1 cut(s) 194
BstHHI GCGC 2 cut(s) 88, 367
BstKTI GATC 1 cut(s) 212
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 2 cut(s) 26, 371
BstNI CCWGG 3 cut(s) 72, 120, 135
BstSCI CCNGG 3 cut(s) 70, 118, 133
BsuRI GGCC 3 cut(s) 123, 260, 289
BtsCI GGATG 1 cut(s) 194
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 2 cut(s) 125, 224
CfoI GCGC 2 cut(s) 88, 367
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 288, 359
CviJI RGCY 7 cut(s) 80, 123, 222, 260, 282, 289, 346
CviKI_1 RGCY 7 cut(s) 80, 123, 222, 260, 282, 289, 346
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 349
Eco147I AGGCCT 2 cut(s) 123, 260
Eco24I GRGCYC 1 cut(s) 348
Eco47I GGWCC 1 cut(s) 359
Eco53kI GAGCTC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 70, 118, 133
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 349
FokI GGATG 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 348
FspBI CTAG 1 cut(s) 38
FspI TGCGCA 1 cut(s) 87
GlaI GCGC 2 cut(s) 87, 366
GsaI CCCAGC 1 cut(s) 374
HaeIII GGCC 3 cut(s) 123, 260, 289
HapII CCGG 1 cut(s) 177
HhaI GCGC 2 cut(s) 88, 367
Hin6I GCGC 2 cut(s) 86, 365
HinP1I GCGC 2 cut(s) 86, 365
HinfI GANTC 2 cut(s) 100, 307
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 144, 306
Hpy188III TCNNGA 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 117, 271
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 3 cut(s) 29, 226, 255
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 371
HspAI GCGC 2 cut(s) 86, 365
Kzo9I GATC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 351
LpnPI CCDG 9 cut(s) 57, 84, 105, 120, 132, 137, 147, 190, 375
MaeI CTAG 1 cut(s) 38
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 2 cut(s) 307, 366
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 2 cut(s) 12, 227
MlyI GAGTC 2 cut(s) 109, 301
MnlI CCTC 6 cut(s) 114, 164, 268, 312, 330, 350
MroXI GAANNNNTTC 1 cut(s) 204
MslI CAYNNNNRTG 1 cut(s) 379
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 3 cut(s) 72, 120, 135
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 3 cut(s) 72, 120, 135
MwoI GCNNNNNNNGC 2 cut(s) 26, 371
NdeII GATC 1 cut(s) 209
NlaIV GGNNCC 3 cut(s) 117, 235, 244
NsbI TGCGCA 1 cut(s) 87
PceI AGGCCT 2 cut(s) 123, 260
PctI GAATGC 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 204
PleI GAGTC 2 cut(s) 108, 301
PpsI GAGTC 2 cut(s) 108, 301
PpuMI RGGWCCY 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 348
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 70, 118, 133
PspFI CCCAGC 1 cut(s) 370
PspGI CCWGG 3 cut(s) 70, 118, 133
PspN4I GGNNCC 3 cut(s) 117, 235, 244
PspPI GGNCC 2 cut(s) 288, 359
PspPPI RGGWCCY 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 379
SacI GAGCTC 1 cut(s) 348
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 2 cut(s) 288, 359
SchI GAGTC 2 cut(s) 109, 301
ScrFI CCNGG 3 cut(s) 72, 120, 135
SduI GDGCHC 1 cut(s) 348
SetI ASST 5 cut(s) 59, 224, 284, 348, 361
SinI GGWCC 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 379
SmlI CTYRAG 2 cut(s) 155, 329
SmoI CTYRAG 2 cut(s) 155, 329
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 2 cut(s) 12, 227
SseBI AGGCCT 2 cut(s) 123, 260
SsiI CCGC 1 cut(s) 113
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 348
StuI AGGCCT 2 cut(s) 123, 260
StyD4I CCNGG 3 cut(s) 70, 118, 133
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 251
TaqI TCGA 2 cut(s) 208, 273
TasI AATT 2 cut(s) 12, 227
TscAI CASTG 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.