Rh6BG143800

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0014822
Physical Location & Seq
Reverse (-)
23020714 .. 23024853
4140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG143800.1

Sequence Viewer

Length: 660 bp
ATGTCTCTACTGAAATCCAATGCCTTCGTCAAAGCATCAAACTCGGGTTCATTGAAGGAAAATTCGGCATTAAAGGAAGGGTCTAACGGTAGCAGCTGTAATCACATTGCAGCACATACATTTACATTTCGCGAATTGGCAGCTGCAACAAAGAATTTTAGAGCAGATTGTTTTGTGGGTGAAGGAGGTTTTGGTCAAGTATATAGAGGGCACCTGGAGAGTTCTAATCAGGTACGGCTTCCATTTTCTTCTTTGAGGCCTATTTTTCGTGCAAAAACTGTATCAGATGCGCCACCATTTGACCCGAGCAATATTGTGTCACTGCAGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGAATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGTTTATCCTAAACTAATGCAAACTATTTGTTTACTGCATCTTAGTACTCTACTGCTGTTTACATGTCACAAACTGACTCTTGCAATTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

219

Amino Acids

24.29

Weight (kDa)

9.58

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 75 - 133 9.9e-07 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 210
AccII CGCG 1 cut(s) 132
AciI CCGC 1 cut(s) 523
AclWI GGATC 1 cut(s) 422
AcsI RAATTY 3 cut(s) 61, 154, 386
AfaI GTAC 3 cut(s) 234, 450, 613
AflIII ACRYGT 2 cut(s) 451, 629
AgsI TTSAA 6 cut(s) 55, 347, 405, 529, 559, 565
AjnI CCWGG 3 cut(s) 213, 440, 487
AjuI GAANNNNNNNTTGG 4 cut(s) 174, 206, 521, 553
AluBI AGCT 4 cut(s) 96, 143, 328, 394
AluI AGCT 4 cut(s) 96, 143, 328, 394
Alw26I GTCTC 2 cut(s) 9, 478
AlwI GGATC 1 cut(s) 422
Ama87I CYCGRG 2 cut(s) 43, 304
AoxI GGCC 2 cut(s) 257, 476
ApeKI GCWGC 5 cut(s) 93, 110, 140, 143, 325
ApoI RAATTY 3 cut(s) 61, 154, 386
AspLEI GCGC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 191
AvaI CYCGRG 2 cut(s) 43, 304
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 1 cut(s) 210
BbvI GCAGC 5 cut(s) 105, 122, 130, 152, 337
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 2 cut(s) 24, 58
BciT130I CCWGG 3 cut(s) 215, 442, 489
BcoDI GTCTC 2 cut(s) 9, 478
BfmI CTRYAG 2 cut(s) 323, 462
BisI GCNGC 5 cut(s) 94, 111, 141, 144, 326
BlsI GCNGC 5 cut(s) 95, 112, 142, 145, 327
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 3 cut(s) 215, 442, 489
BmeT110I CYCGRG 2 cut(s) 43, 304
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 3 cut(s) 215, 442, 489
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 3 cut(s) 44, 277, 613
BmuI ACTGGG 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 236
BsaAI YACGTR 1 cut(s) 454
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 1 cut(s) 440
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 3 cut(s) 215, 442, 489
BseDI CCNNGG 1 cut(s) 440
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 5 cut(s) 105, 122, 130, 152, 337
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 259, 478
BshNI GGYRCC 1 cut(s) 210
BsiHKCI CYCGRG 2 cut(s) 43, 304
BsmAI GTCTC 2 cut(s) 9, 478
BsnI GGCC 2 cut(s) 259, 478
Bso31I GGTCTC 1 cut(s) 478
BsoBI CYCGRG 2 cut(s) 43, 304
Bsp1286I GDGCHC 1 cut(s) 213
Bsp1407I TGTACA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 427, 496
Bsp68I TCGCGA 1 cut(s) 132
BspACI CCGC 1 cut(s) 523
BspANI GGCC 2 cut(s) 259, 478
BspCNI CTCAG 1 cut(s) 472
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 212
BspMAI CTGCAG 2 cut(s) 327, 466
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 105
BsrGI TGTACA 1 cut(s) 448
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 440
BssMI GATC 2 cut(s) 427, 496
Bst2UI CCWGG 3 cut(s) 215, 442, 489
Bst4CI ACNGT 2 cut(s) 89, 280
BstAUI TGTACA 1 cut(s) 448
BstBAI YACGTR 1 cut(s) 454
BstDEI CTNAG 3 cut(s) 480, 500, 608
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 2 cut(s) 430, 499
BstMAI GTCTC 2 cut(s) 9, 478
BstMBI GATC 2 cut(s) 427, 496
BstNI CCWGG 3 cut(s) 215, 442, 489
BstNSI RCATGY 1 cut(s) 633
BstSCI CCNGG 3 cut(s) 213, 440, 487
BstSFI CTRYAG 2 cut(s) 323, 462
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 5 cut(s) 105, 122, 130, 152, 337
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 2 cut(s) 259, 478
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 1 cut(s) 320
BtuMI TCGCGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 233, 449, 612
CviAII CATG 1 cut(s) 630
CviJI RGCY 8 cut(s) 96, 143, 238, 259, 328, 394, 459, 478
CviKI_1 RGCY 8 cut(s) 96, 143, 238, 259, 328, 394, 459, 478
CviQI GTAC 3 cut(s) 233, 449, 612
DdeI CTNAG 3 cut(s) 480, 500, 608
DpnI GATC 2 cut(s) 429, 498
DpnII GATC 2 cut(s) 427, 496
Eco147I AGGCCT 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 478
Eco88I CYCGRG 2 cut(s) 43, 304
EcoRII CCWGG 3 cut(s) 213, 440, 487
FaeI CATG 1 cut(s) 633
FaiI YATR 8 cut(s) 117, 202, 204, 332, 351, 410, 434, 631
FatI CATG 1 cut(s) 629
Fnu4HI GCNGC 5 cut(s) 94, 111, 141, 144, 326
Fsp4HI GCNGC 5 cut(s) 94, 111, 141, 144, 326
GlaI GCGC 1 cut(s) 291
GluI GCNGC 5 cut(s) 94, 111, 141, 144, 326
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 2 cut(s) 259, 478
HhaI GCGC 1 cut(s) 292
Hin1II CATG 1 cut(s) 633
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HinfI GANTC 1 cut(s) 643
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 4 cut(s) 451, 506, 599, 627
Hpy188I TCNGA 2 cut(s) 286, 423
Hpy188III TCNNGA 2 cut(s) 131, 550
Hpy8I GTNNAC 4 cut(s) 451, 506, 599, 627
HpyAV CCTTC 6 cut(s) 34, 49, 71, 176, 374, 559
HpyCH4III ACNGT 2 cut(s) 89, 280
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 8 cut(s) 110, 146, 272, 325, 464, 586, 604, 650
HpyF3I CTNAG 3 cut(s) 480, 500, 608
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 1 cut(s) 633
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 2 cut(s) 427, 496
LmnI GCTCC 1 cut(s) 399
Lsp1109I GCAGC 5 cut(s) 105, 122, 130, 152, 337
LweI GCATC 3 cut(s) 44, 277, 613
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 318, 469, 632
MalI GATC 2 cut(s) 429, 498
MboI GATC 2 cut(s) 427, 496
MboII GAAGA 1 cut(s) 240
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 7 cut(s) 61, 134, 154, 386, 536, 560, 651
MlyI GAGTC 1 cut(s) 637
MnlI CCTC 4 cut(s) 179, 200, 249, 522
MseI TTAA 3 cut(s) 71, 390, 654
MspA1I CMGCKG 2 cut(s) 96, 143
MspR9I CCNGG 3 cut(s) 215, 442, 489
MvaI CCWGG 3 cut(s) 215, 442, 489
MvnI CGCG 1 cut(s) 132
NdeII GATC 2 cut(s) 427, 496
NlaIII CATG 1 cut(s) 633
NlaIV GGNNCC 1 cut(s) 212
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 2 cut(s) 318, 632
NruI TCGCGA 1 cut(s) 132
NspI RCATGY 1 cut(s) 633
PceI AGGCCT 1 cut(s) 259
PciI ACATGT 1 cut(s) 629
PkrI GCNGC 5 cut(s) 95, 112, 142, 145, 327
PleI GAGTC 1 cut(s) 637
PpsI GAGTC 1 cut(s) 637
Ppu21I YACGTR 1 cut(s) 454
PscI ACATGT 1 cut(s) 629
Psp6I CCWGG 3 cut(s) 213, 440, 487
PspGI CCWGG 3 cut(s) 213, 440, 487
PspN4I GGNNCC 1 cut(s) 212
PstI CTGCAG 2 cut(s) 327, 466
PsuI RGATCY 1 cut(s) 427
PvuII CAGCTG 2 cut(s) 96, 143
RruI TCGCGA 1 cut(s) 132
RsaI GTAC 3 cut(s) 234, 450, 613
RsaNI GTAC 3 cut(s) 233, 449, 612
SaqAI TTAA 3 cut(s) 71, 390, 654
SatI GCNGC 5 cut(s) 94, 111, 141, 144, 326
Sau3AI GATC 2 cut(s) 427, 496
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 1 cut(s) 637
ScrFI CCNGG 3 cut(s) 215, 442, 489
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 3 cut(s) 44, 277, 613
SfcI CTRYAG 2 cut(s) 323, 462
Sse9I AATT 7 cut(s) 61, 134, 154, 386, 536, 560, 651
SseBI AGGCCT 1 cut(s) 259
SsiI CCGC 1 cut(s) 523
SspI AATATT 1 cut(s) 313
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 3 cut(s) 213, 440, 487
TaaI ACNGT 2 cut(s) 89, 280
TaiI ACGT 1 cut(s) 456
TasI AATT 7 cut(s) 61, 134, 154, 386, 536, 560, 651
TatI WGTACW 2 cut(s) 448, 611
Tru1I TTAA 3 cut(s) 71, 390, 654
Tru9I TTAA 3 cut(s) 71, 390, 654
TscAI CASTG 1 cut(s) 327
TseFI GTSAC 2 cut(s) 318, 632
TseI GCWGC 5 cut(s) 93, 110, 140, 143, 325
Tsp45I GTSAC 2 cut(s) 318, 632
TspDTI ATGAA 1 cut(s) 39
TspRI CASTG 1 cut(s) 327
XapI RAATTY 3 cut(s) 61, 154, 386
XceI RCATGY 1 cut(s) 633
ZrmI AGTACT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.