Rorug04G0064300

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014822
Physical Location & Seq
Forward (+)
9985392 .. 9987569
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0064300.1

Sequence Viewer

Length: 1059 bp
ATGGGGAAAAAGATGAAGAATAAAACGGACAGGGAGACGATTGATTCTAATCCGATTATTGATTTGAAGTCTATTATCCATCAGCATTCCCAGTTCATGGACAAGCTAGTGGAGCTCATCCCTGCTAGGTTCTATTTACCCAATGACGACGACGAGAAGCCATGGTTTCAAGGCCTCAGCAACCCTGCAAAAGCTTCAGCTAAGAAGGAAACGAAGAAAAACATCAAGAAAGCTCGCAGAGATCGATTGGATCCAGAGAAGACTTCTACAACAACCCTTGATTTGCTAAAGCAAAGTTTGGAAAAGGAGAAGTCGAATGAGGATGAGAGTGATAGTGGTGAAATGGAGACTAAACCTGTGGTGCCTGGGCTGGAAGGTGACGACAGGTCAGTGACATATGAAGAACTCCAGCAACGGCTTCGTCGTAAAATTGAACAGTTTCAGTCTGGTCGCAATTGTGGAGTTTCAGAGAGAGCAAGGAAGAGGAGCGAAAGGAAGGAGAGATATGAGAAAGGAGCTGATGACAGGAAACGGAAGAGAGAAACTGCATCTGATGAAAAGAAACCTACTAGTAGTGAGTTGGTGGACAAGAAAGTTGAAGAAGACGTTGCAGAGGCTTCTAAAGGGCTCACATTCAGTCGTGTCAAACTTGTGGGTGATGATGAGGTTGGAAAGAAGAAGAGAAGACTGTCAAAGGCGAAGGAGCTTGAGAGGGCCAAACAGTTGGAAGAAGTGAAGAAAGATCCAGAGAAAGGCAAGTCTCTCTTGTGGAAAACAGCAACCAGTAGAGCTGCTGGGGTTAAGGTTCATGATGATCCAAAGTTGCTGAAACAAAGCATAAAAAAGGAGAACAAGAAGCATCAGAAGAATGCTGAGAAATGGAAGGAAAGGGTTGAAACCACACACAAGTTGAAAGCAGACAAACAGAAGAAGAGATCTGATAATATTTCAGCAAAACTTGATGAGAAGAAAAAGCGCAAAATCGCAAAGAGAGAGAAGAAACTCATGCGGCCAGGATTTGAAGGTCGCAAAGAAGGTTATGTCAATGAGGGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

352

Amino Acids

40.73

Weight (kDa)

9.77

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP14 PF15459 26 - 86 1.3e-20 60S ribosome biogenesis protein Rrp14
SURF6 PF04935 157 - 327 2.7e-19 Surfeit locus protein 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 97
AciI CCGC 1 cut(s) 1009
AclWI GGATC 4 cut(s) 245, 258, 737, 809
AcoI YGGCCR 1 cut(s) 1010
AcuI CTGAAG 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 97, 752
AgsI TTSAA 7 cut(s) 67, 170, 434, 599, 896, 913, 1022
AhdI GACNNNNNGTC 1 cut(s) 385
AhlI ACTAGT 1 cut(s) 569
AjnI CCWGG 2 cut(s) 364, 1012
AluBI AGCT 8 cut(s) 106, 115, 194, 200, 233, 518, 706, 791
AluI AGCT 8 cut(s) 106, 115, 194, 200, 233, 518, 706, 791
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 3 cut(s) 29, 341, 765
AlwI GGATC 4 cut(s) 245, 258, 737, 809
AoxI GGCC 3 cut(s) 172, 714, 1010
ApeKI GCWGC 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 438
AspLEI GCGC 1 cut(s) 978
AspS9I GGNCC 1 cut(s) 714
AsuHPI GGTGA 3 cut(s) 350, 389, 668
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 2 cut(s) 117, 630
BbsI GAAGAC 3 cut(s) 266, 609, 691
Bbv12I GWGCWC 1 cut(s) 117
BbvCI CCTCAGC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 778
BccI CCATC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 431
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 2 cut(s) 366, 1014
BcoDI GTCTC 3 cut(s) 29, 341, 765
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 3 cut(s) 107, 126, 570
BglII AGATCT 1 cut(s) 935
BisI GCNGC 2 cut(s) 792, 1010
BlsI GCNGC 2 cut(s) 793, 1011
Bme1390I CCNGG 2 cut(s) 366, 1014
BmeRI GACNNNNNGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 714
BmiI GGNNCC 2 cut(s) 252, 363
BmrFI CCNGG 2 cut(s) 366, 1014
BmrI ACTGGG 1 cut(s) 85
BmsI GCATC 2 cut(s) 557, 868
BmuI ACTGGG 1 cut(s) 85
BpiI GAAGAC 3 cut(s) 266, 609, 691
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 728
Bsa29I ATCGAT 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 161, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 752
Bse1I ACTGG 2 cut(s) 91, 783
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 2 cut(s) 366, 1014
BseCI ATCGAT 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 161, 365
BseGI GGATG 2 cut(s) 117, 328
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 97, 752
BseMII CTCAG 2 cut(s) 190, 864
BseNI ACTGG 2 cut(s) 91, 783
BseRI GAGGAG 1 cut(s) 499
BseXI GCAGC 1 cut(s) 778
BseYI CCCAGC 1 cut(s) 794
BshFI GGCC 3 cut(s) 174, 716, 1012
BshNI GGYRCC 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 97, 752
BsmAI GTCTC 3 cut(s) 29, 341, 765
BsmBI CGTCTC 1 cut(s) 29
BsmI GAATGC 2 cut(s) 85, 874
BsnI GGCC 3 cut(s) 174, 716, 1012
Bsp1286I GDGCHC 2 cut(s) 117, 630
Bsp143I GATC 5 cut(s) 241, 250, 742, 814, 935
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 1 cut(s) 1009
BspANI GGCC 3 cut(s) 174, 716, 1012
BspCNI CTCAG 2 cut(s) 189, 865
BspDI ATCGAT 1 cut(s) 244
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 2 cut(s) 252, 363
BspPI GGATC 4 cut(s) 245, 258, 737, 809
BspT107I GGYRCC 1 cut(s) 361
BsrI ACTGG 2 cut(s) 91, 783
BssECI CCNNGG 2 cut(s) 161, 365
BssMI GATC 5 cut(s) 241, 250, 742, 814, 935
BssT1I CCWWGG 1 cut(s) 161
Bst2UI CCWGG 2 cut(s) 366, 1014
Bst4CI ACNGT 3 cut(s) 438, 690, 723
Bst6I CTCTTC 4 cut(s) 476, 530, 674, 926
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 3 cut(s) 176, 201, 873
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 2 cut(s) 117, 328
BstHHI GCGC 1 cut(s) 978
BstKTI GATC 5 cut(s) 244, 253, 745, 817, 938
BstMAI GTCTC 3 cut(s) 29, 341, 765
BstMBI GATC 5 cut(s) 241, 250, 742, 814, 935
BstMWI GCNNNNNNNGC 1 cut(s) 112
BstNI CCWGG 2 cut(s) 366, 1014
BstSCI CCNGG 2 cut(s) 364, 1012
BstV1I GCAGC 1 cut(s) 778
BstV2I GAAGAC 3 cut(s) 266, 609, 691
BstX2I RGATCY 3 cut(s) 250, 742, 935
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 3 cut(s) 250, 742, 935
Bsu15I ATCGAT 1 cut(s) 244
BsuRI GGCC 3 cut(s) 174, 716, 1012
BsuTUI ATCGAT 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 2 cut(s) 117, 328
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 1 cut(s) 235
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 978
Cfr13I GGNCC 1 cut(s) 714
ClaI ATCGAT 1 cut(s) 244
CviAII CATG 4 cut(s) 97, 162, 809, 1006
DdeI CTNAG 3 cut(s) 176, 201, 873
DpnI GATC 5 cut(s) 243, 252, 744, 816, 937
DpnII GATC 5 cut(s) 241, 250, 742, 814, 935
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 1010
Eam1104I CTCTTC 4 cut(s) 476, 530, 674, 926
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 4 cut(s) 476, 530, 674, 926
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 161
Eco147I AGGCCT 1 cut(s) 174
Eco24I GRGCYC 2 cut(s) 117, 630
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 364, 1012
EcoT14I CCWWGG 1 cut(s) 161
EcoT38I GRGCYC 2 cut(s) 117, 630
ErhI CCWWGG 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 4 cut(s) 100, 165, 812, 1009
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FatI CATG 4 cut(s) 96, 161, 808, 1005
FauNDI CATATG 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 792, 1010
FokI GGATG 2 cut(s) 104, 335
FriOI GRGCYC 2 cut(s) 117, 630
Fsp4HI GCNGC 2 cut(s) 792, 1010
FspBI CTAG 3 cut(s) 107, 126, 570
GlaI GCGC 1 cut(s) 977
GluI GCNGC 2 cut(s) 792, 1010
GsaI CCCAGC 1 cut(s) 798
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 3 cut(s) 174, 716, 1012
HhaI GCGC 1 cut(s) 978
Hin1II CATG 4 cut(s) 100, 165, 812, 1009
Hin6I GCGC 1 cut(s) 976
HinP1I GCGC 1 cut(s) 976
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 1 cut(s) 44
HphI GGTGA 3 cut(s) 350, 389, 668
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 5 cut(s) 54, 469, 553, 864, 940
Hpy188III TCNNGA 4 cut(s) 226, 254, 746, 809
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 3 cut(s) 152, 155, 426
HpyAV CCTTC 7 cut(s) 199, 368, 490, 694, 877, 1016, 1028
HpyCH4III ACNGT 3 cut(s) 438, 690, 723
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 4 cut(s) 188, 548, 611, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 112
HpyF3I CTNAG 3 cut(s) 176, 201, 873
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 4 cut(s) 100, 165, 812, 1009
HspAI GCGC 1 cut(s) 976
Kzo9I GATC 5 cut(s) 241, 250, 742, 814, 935
LmnI GCTCC 4 cut(s) 112, 486, 515, 703
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 2 cut(s) 557, 868
MaeI CTAG 3 cut(s) 107, 126, 570
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 2 cut(s) 377, 391
MalI GATC 5 cut(s) 243, 252, 744, 816, 937
MboI GATC 5 cut(s) 241, 250, 742, 814, 935
MfeI CAATTG 1 cut(s) 454
MflI RGATCY 3 cut(s) 250, 742, 935
MhlI GDGCHC 2 cut(s) 117, 630
MluCI AATT 2 cut(s) 429, 454
MmeI TCCRAC 2 cut(s) 649, 705
MnlI CCTC 7 cut(s) 185, 313, 477, 607, 658, 705, 1042
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 1 cut(s) 801
MspR9I CCNGG 2 cut(s) 366, 1014
MunI CAATTG 1 cut(s) 454
Mva1269I GAATGC 2 cut(s) 85, 874
MvaI CCWGG 2 cut(s) 366, 1014
MwoI GCNNNNNNNGC 1 cut(s) 112
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 397
NdeII GATC 5 cut(s) 241, 250, 742, 814, 935
NlaIII CATG 4 cut(s) 100, 165, 812, 1009
NlaIV GGNNCC 2 cut(s) 252, 363
NmuCI GTSAC 2 cut(s) 377, 391
PagI TCATGA 1 cut(s) 808
PceI AGGCCT 1 cut(s) 174
PcsI WCGNNNNNNNCGW 2 cut(s) 241, 421
PctI GAATGC 2 cut(s) 85, 874
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 2 cut(s) 793, 1011
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 364, 1012
PspFI CCCAGC 1 cut(s) 794
PspGI CCWGG 2 cut(s) 364, 1012
PspN4I GGNNCC 2 cut(s) 252, 363
PspPI GGNCC 1 cut(s) 714
PsuI RGATCY 3 cut(s) 250, 742, 935
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 2 cut(s) 792, 1010
Sau3AI GATC 5 cut(s) 241, 250, 742, 814, 935
Sau96I GGNCC 1 cut(s) 714
ScrFI CCNGG 2 cut(s) 366, 1014
SduI GDGCHC 2 cut(s) 117, 630
SfaNI GCATC 2 cut(s) 557, 868
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
SpeI ACTAGT 1 cut(s) 569
Sse9I AATT 2 cut(s) 429, 454
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 1 cut(s) 1009
SspI AATATT 1 cut(s) 946
SspMI CTAG 3 cut(s) 107, 126, 570
SstI GAGCTC 1 cut(s) 117
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 364, 1012
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 3 cut(s) 438, 690, 723
TaiI ACGT 1 cut(s) 609
TaqI TCGA 2 cut(s) 244, 314
TasI AATT 2 cut(s) 429, 454
TauI GCSGC 1 cut(s) 1012
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 2 cut(s) 377, 391
TseI GCWGC 1 cut(s) 791
Tsp45I GTSAC 2 cut(s) 377, 391
TspDTI ATGAA 5 cut(s) 29, 85, 414, 570, 797
TspGWI ACGGA 2 cut(s) 41, 547
TspRI CASTG 1 cut(s) 396
Van91I CCANNNNNTGG 1 cut(s) 97
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 107, 126, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.