Rorug02G0523600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007432
Physical Location & Seq
Forward (+)
65439111 .. 65440811
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0523600.1

Sequence Viewer

Length: 501 bp
ATGGACGAGGTGATGACGGCTGCCGATGCGACTTCCGGCGGCGCCACGACGTCGTCACCGGCATGGATCCCGGCCAACCTGGGTCTCATCTCCGCTTTCCTCGCCTGCGCCGTCGCTCAGTTCCTCAAGCTCTTCACGTCCTGGTATAAAGATAGGAGATGGGATTCGAAACGAATGCTGGATTCTGGAGGAATGCCGTCGTCGCATTCGGCGACGGTGACGGCGCTGACGGTGGCCGTTGGGCTAGACCAGGGCACCGGAGGAGCGGCTTTTGCTCTTGCCCTGGTCTTAGCGTTAATTGTAATGTATGATGCCACAGGAGTGAGACTTCATGCAGGTCGCCAAGCCGAATTGCTGAACCAAATTGTGTGTGAGCTTCCTCCGGAGCATCCTTTGTCCGCTGTTAGACCCTTGCGTGATTCGCTTGGACATACTCCAGTACAGGTTGTTGCTGGTGCTATTTTGGGATGCATAGTAGCATATCTAATGAGGGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

17.33

Weight (kDa)

6.39

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 26 - 159 4.6e-50 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 326
AccB1I GGYRCC 2 cut(s) 41, 254
AccBSI CCGCTC 1 cut(s) 266
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 4 cut(s) 39, 93, 266, 399
AclWI GGATC 2 cut(s) 61, 74
AcoI YGGCCR 2 cut(s) 72, 234
AcyI GRCGYC 2 cut(s) 42, 50
AfaI GTAC 1 cut(s) 441
AjiI CACGTC 1 cut(s) 138
AjnI CCWGG 4 cut(s) 78, 140, 249, 282
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 2 cut(s) 130, 376
AluI AGCT 2 cut(s) 130, 376
Alw26I GTCTC 2 cut(s) 89, 319
AlwI GGATC 2 cut(s) 61, 74
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 2 cut(s) 72, 234
ApeKI GCWGC 2 cut(s) 20, 494
AspLEI GCGC 3 cut(s) 44, 110, 226
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 3 cut(s) 22, 48, 229
AsuII TTCGAA 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 257
BamHI GGATCC 1 cut(s) 66
BanI GGYRCC 2 cut(s) 41, 254
BbvI GCAGC 2 cut(s) 7, 481
BccI CCATC 1 cut(s) 153
BceAI ACGGC 5 cut(s) 33, 95, 181, 221, 237
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 4 cut(s) 80, 142, 251, 284
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 2 cut(s) 89, 319
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 2 cut(s) 45, 227
BfuAI ACCTGC 1 cut(s) 326
BisI GCNGC 4 cut(s) 21, 40, 267, 495
BlsI GCNGC 4 cut(s) 22, 41, 268, 496
Bme1390I CCNGG 5 cut(s) 71, 80, 142, 251, 284
BmgBI CACGTC 1 cut(s) 138
BmiI GGNNCC 3 cut(s) 43, 68, 256
BmrFI CCNGG 5 cut(s) 71, 80, 142, 251, 284
BmsI GCATC 4 cut(s) 16, 301, 397, 458
BpmI CTGGAG 2 cut(s) 207, 420
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 71
BsaHI GRCGYC 2 cut(s) 42, 50
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 79, 250, 282
BsaWI WCCGGW 2 cut(s) 257, 382
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 437
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 4 cut(s) 80, 142, 251, 284
BseDI CCNNGG 3 cut(s) 79, 250, 282
BseGI GGATG 2 cut(s) 388, 473
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 2 cut(s) 7, 481
BshFI GGCC 2 cut(s) 74, 236
BshNI GGYRCC 2 cut(s) 41, 254
BsiSI CCGG 5 cut(s) 36, 59, 71, 258, 383
BsmAI GTCTC 2 cut(s) 89, 319
BsmI GAATGC 3 cut(s) 180, 198, 205
BsnI GGCC 2 cut(s) 74, 236
Bso31I GGTCTC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 257
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 4 cut(s) 39, 93, 266, 399
BspANI GGCC 2 cut(s) 74, 236
BspCNI CTCAG 1 cut(s) 130
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 43, 68, 256
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 2 cut(s) 61, 74
BspQI GCTCTTC 1 cut(s) 137
BspT104I TTCGAA 1 cut(s) 167
BspT107I GGYRCC 2 cut(s) 41, 254
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 1 cut(s) 437
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 3 cut(s) 79, 250, 282
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 2 cut(s) 42, 50
Bst2UI CCWGG 4 cut(s) 80, 142, 251, 284
Bst4CI ACNGT 2 cut(s) 217, 232
Bst6I CTCTTC 1 cut(s) 137
BstACI GRCGYC 2 cut(s) 42, 50
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 3 cut(s) 117, 289, 498
BstF5I GGATG 2 cut(s) 388, 473
BstH2I RGCGCY 2 cut(s) 45, 227
BstHHI GCGC 3 cut(s) 44, 110, 226
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 2 cut(s) 89, 319
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 5 cut(s) 26, 101, 202, 272, 421
BstNI CCWGG 4 cut(s) 80, 142, 251, 284
BstSCI CCNGG 5 cut(s) 69, 78, 140, 249, 282
BstSLI GKGCMC 1 cut(s) 257
BstV1I GCAGC 2 cut(s) 7, 481
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 2 cut(s) 74, 236
BtrI CACGTC 1 cut(s) 138
BtsCI GGATG 2 cut(s) 388, 473
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 3 cut(s) 44, 110, 226
Cfr10I RCCGGY 1 cut(s) 58
Csp6I GTAC 1 cut(s) 440
CviAII CATG 2 cut(s) 63, 332
CviJI RGCY 9 cut(s) 20, 74, 130, 236, 244, 269, 347, 376, 494
CviKI_1 RGCY 9 cut(s) 20, 74, 130, 236, 244, 269, 347, 376, 494
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 3 cut(s) 117, 289, 498
DinI GGCGCC 1 cut(s) 43
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
EaeI YGGCCR 2 cut(s) 72, 234
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 89
EcoRII CCWGG 4 cut(s) 78, 140, 249, 282
EcoT22I ATGCAT 1 cut(s) 473
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
FaeI CATG 2 cut(s) 66, 335
FaiI YATR 7 cut(s) 64, 147, 309, 333, 432, 473, 481
FatI CATG 2 cut(s) 62, 331
Fnu4HI GCNGC 4 cut(s) 21, 40, 267, 495
FokI GGATG 2 cut(s) 375, 480
Fsp4HI GCNGC 4 cut(s) 21, 40, 267, 495
FspBI CTAG 1 cut(s) 245
GlaI GCGC 3 cut(s) 43, 109, 225
GluI GCNGC 4 cut(s) 21, 40, 267, 495
GsuI CTGGAG 2 cut(s) 207, 420
HaeII RGCGCY 2 cut(s) 45, 227
HaeIII GGCC 2 cut(s) 74, 236
HapII CCGG 5 cut(s) 36, 59, 71, 258, 383
HhaI GCGC 3 cut(s) 44, 110, 226
Hin1I GRCGYC 2 cut(s) 42, 50
Hin1II CATG 2 cut(s) 66, 335
Hin6I GCGC 3 cut(s) 42, 108, 224
HinP1I GCGC 3 cut(s) 42, 108, 224
HinfI GANTC 3 cut(s) 164, 182, 419
HpaII CCGG 5 cut(s) 36, 59, 71, 258, 383
HphI GGTGA 3 cut(s) 22, 48, 229
Hpy188III TCNNGA 2 cut(s) 186, 383
Hpy99I CGWCG 6 cut(s) 52, 55, 116, 202, 205, 217
HpyCH4III ACNGT 2 cut(s) 217, 232
HpyCH4IV ACGT 2 cut(s) 50, 137
HpyCH4V TGCA 2 cut(s) 335, 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 101, 202, 272, 421
HpyF3I CTNAG 3 cut(s) 117, 289, 498
HpySE526I ACGT 2 cut(s) 50, 137
Hsp92I GRCGYC 2 cut(s) 42, 50
Hsp92II CATG 2 cut(s) 66, 335
HspAI GCGC 3 cut(s) 42, 108, 224
KasI GGCGCC 1 cut(s) 41
Kpn2I TCCGGA 1 cut(s) 382
Kzo9I GATC 1 cut(s) 66
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 263, 385
Lsp1109I GCAGC 2 cut(s) 7, 481
LweI GCATC 4 cut(s) 16, 301, 397, 458
MaeI CTAG 1 cut(s) 245
MaeII ACGT 2 cut(s) 50, 137
MaeIII GTNAC 2 cut(s) 54, 217
MalI GATC 1 cut(s) 68
MbiI CCGCTC 1 cut(s) 266
MboI GATC 1 cut(s) 66
MboII GAAGA 1 cut(s) 124
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 3 cut(s) 297, 350, 363
Mly113I GGCGCC 1 cut(s) 42
MnlI CCTC 6 cut(s) 110, 134, 182, 254, 390, 483
Mph1103I ATGCAT 1 cut(s) 473
MroI TCCGGA 1 cut(s) 382
MseI TTAA 1 cut(s) 296
MslI CAYNNNNRTG 2 cut(s) 61, 320
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 5 cut(s) 36, 59, 71, 258, 383
MspR9I CCNGG 5 cut(s) 71, 80, 142, 251, 284
Mva1269I GAATGC 3 cut(s) 180, 198, 205
MvaI CCWGG 4 cut(s) 80, 142, 251, 284
MwoI GCNNNNNNNGC 5 cut(s) 26, 101, 202, 272, 421
NarI GGCGCC 1 cut(s) 42
NciI CCSGG 1 cut(s) 71
NdeII GATC 1 cut(s) 66
NlaIII CATG 2 cut(s) 66, 335
NlaIV GGNNCC 3 cut(s) 43, 68, 256
NmuCI GTSAC 2 cut(s) 54, 217
NsiI ATGCAT 1 cut(s) 473
NspV TTCGAA 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 209
PctI GAATGC 3 cut(s) 180, 198, 205
PfeI GAWTC 3 cut(s) 164, 182, 419
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 4 cut(s) 22, 41, 268, 496
PluTI GGCGCC 1 cut(s) 45
Psp6I CCWGG 4 cut(s) 78, 140, 249, 282
PspGI CCWGG 4 cut(s) 78, 140, 249, 282
PspN4I GGNNCC 3 cut(s) 43, 68, 256
PsuI RGATCY 1 cut(s) 66
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
RseI CAYNNNNRTG 2 cut(s) 61, 320
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 4 cut(s) 21, 40, 267, 495
Sau3AI GATC 1 cut(s) 66
ScrFI CCNGG 5 cut(s) 71, 80, 142, 251, 284
SduI GDGCHC 1 cut(s) 257
SetI ASST 8 cut(s) 12, 53, 81, 132, 140, 340, 378, 447
SfaNI GCATC 4 cut(s) 16, 301, 397, 458
SfoI GGCGCC 1 cut(s) 43
SfuI TTCGAA 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 61, 320
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 3 cut(s) 297, 350, 363
SsiI CCGC 4 cut(s) 39, 93, 266, 399
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 5 cut(s) 69, 78, 140, 249, 282
TaaI ACNGT 2 cut(s) 217, 232
TaiI ACGT 2 cut(s) 53, 140
TaqI TCGA 1 cut(s) 167
TasI AATT 3 cut(s) 297, 350, 363
TatI WGTACW 1 cut(s) 439
TauI GCSGC 2 cut(s) 42, 269
TfiI GAWTC 3 cut(s) 164, 182, 419
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TseFI GTSAC 2 cut(s) 54, 217
TseI GCWGC 2 cut(s) 20, 494
Tsp45I GTSAC 2 cut(s) 54, 217
TspDTI ATGAA 1 cut(s) 320
Tth111I GACNNNGTC 1 cut(s) 52
XspI CTAG 1 cut(s) 245
ZraI GACGTC 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.