Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001835

Physical Location & Seq

Genomic Coordinates Reverse (-)

68385399 .. 68389614

Gene Structure 4216 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0557300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGAAGCACTACCAAGCCAATCTTCATGATCATGAGCTTCCCTCTGGGAAATACCAGGCACTTATACTAAAGCCTCCCAAAAAAATTGTTCTTCTAGTCTTTGCCCTACTTCTTCTTACAATAATCCCATTCTATTACTCTTCATTAAGCAACTCTTTTTTCCTTAGAAAAACTATTCCAAACCCACCATCACTACCCTCTTCTCCATCATATGATGAAGAAGCTCATGAGGAGTTGCCAACCATAACTAATAATCTAAAGTGTGACATATTTAGTGGAGAATGGATTCCAAATCCAGAGGCTCCCTATTACACAAACACGACATGTTGGGCGATTCACGAGCATCAGAACTGCCAGAAATATGGAAGACCAGACTCAGAGTTCATGAAATGGAAGTGGAAGCCCGACGGGTGTGAACTGCCCGTTTTCAACCCGGCTCAGTTCCTTGAATTGCTCCGAGGGAAATCATTGGCCTTCGTTGGAGATTCTGTGGGTAGAAATCAAATGCAGTCACTGATATGCCTTCTTTCTAGGGTGGAATATCCGATAGATGTTTCATACACAAAGGATGAGAAATACAAACGCTGGAAATACACATCCTACAACTTCACCATGGCCTCATTTTGGACGCCACATTTGATCAAATCCAAAGAAGAAGACAGTCATGGTCCAACCAACACTGGTATTTTCGGTCTCTACCTCGACGAACTCGATGAGGCATGGACAAGCCAAGTTGAGGACTTTGATTACCTCATTATTTCCGGAGGGCATTGGTTCATGCGATCCATGGTGTTCCATGAAAATAACAAAATCTCAGGTTGTCACTATTGCCTCTTAGACAATGTGCCTGACTTGGGAATGTTCTACGGCTACAGAAAGGCGTTTAGGACAGCTTTTAAGGCCATCAACAGCTTGAAAAATTACAAGGGTATAACGTACCTTAGGACTTTTGCACCATCACATTTTGAGAATGGGATGTGGAACAAAGGAGGGAATTGTGTGAGAACGAAGCCGTTTAGGAGCAATGAGACCCAATTGGAGGGTGCCAGTTTGGAGTTTTACATGATTCAATTAGAGGAATTTAGGACTGCTGAGAAGGAAGGGAGAAAGAAGGGGTTAAAATATAGGTTATTGGACACAACACAAGCCACGTTACTAAGGCCAGATGGTCATCCTAGTAGATATGGGCATTGGCCAGAGGAAAATGTGACTTTGTATAATGACTGTGTGCATTGGTGCCTGCCAGGACCTATTGATACTTGGGGTGATTTCTTGCTTGAGATGTTGAAGATGGAGGGTATCAGATCAGCTGAGGAGAAGCGCTATTCAAGAGATAGAAGAATGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0080090 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

449

Amino Acids

52.42

Weight (kDa)

6.97

Isoelectric Point (pI)

38.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	87 - 139	5.5e-21	PMR5 N terminal Domain
PC-Esterase	PF13839	140 - 428	2.4e-94	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001835)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14720 AT5G14720
fragaria_vesca	FvH4_4g34360 FvH4_4g34360 FvH4_6g50820 FvH4_6g50820 FvH4_6g50820 FvH4_6g50820 FvH4_6g50820
malus_domestica	MD09G1031900.v1.1 MD09G1032000.v1.1 MD16G1093600.v1.1 MD17G1033700.v1.1
prunus_persica	Prupe.1G252900_v2.0.a1 Prupe.1G252900_v2.0.a1 Prupe.1G252900_v2.0.a1 Prupe.1G252900_v2.0.a1 Prupe.1G252900_v2.0.a1 Prupe.3G286000_v2.0.a1 Prupe.3G286000_v2.0.a1 Prupe.3G286000_v2.0.a1 Prupe.3G286000_v2.0.a1
pyrus_communis	pycom111g02520 pycom16g07960 pycom17g03080
rosa_chinensis	RchiOBHm_Chr2g0172211 RchiOBHm_Chr4g0443771
rosa_laevigata	RLG00000005891 RLG00000022093
rosa_multiflora	Rmu_co8491651.1_g000001 Rmu_ssc0000252.1_g000052
rosa_roxburghii	Rroxscaffold_2G00080080 Rroxscaffold_5G00384340
rosa_rugosa	Rorug02G0557300 Rorug02G0557400 Rorug04G0347100
rosa_samantha	Rh2AG632700 Rh2BG646300 Rh2CG612900 Rh2DG653800 Rh2DG660900 Rh4AG407400 Rh4BG418300 Rh4CG432400 Rh4DG413600
rosa_wichuraiana	Rw2G052380 Rw4G034990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1043, 1236
AccIII	TCCGGA	1 cut(s)	761
AclWI	GGATC	1 cut(s)	777
AcoI	YGGCCR	1 cut(s)	1193
AcsI	RAATTY	1 cut(s)	1079
AcyI	GRCGYC	1 cut(s)	629
AfaI	GTAC	1 cut(s)	938
AfeI	AGCGCT	1 cut(s)	1322
AfiI	CCNNNNNNNGG	4 cut(s)	624, 736, 854, 1039
AflIII	ACRYGT	1 cut(s)	323
AgsI	TTSAA	6 cut(s)	430, 449, 916, 1070, 1288, 1329
AjnI	CCWGG	2 cut(s)	54, 1243
AjuI	GAANNNNNNNTTGG	1 cut(s)	38
AluBI	AGCT	5 cut(s)	37, 224, 893, 912, 1310
AluI	AGCT	5 cut(s)	37, 224, 893, 912, 1310
Alw26I	GTCTC	2 cut(s)	698, 1022
AlwI	GGATC	1 cut(s)	777
AlwNI	CAGNNNCTG	1 cut(s)	514
Aor13HI	TCCGGA	1 cut(s)	761
Aor51HI	AGCGCT	1 cut(s)	1322
AoxI	GGCC	5 cut(s)	471, 615, 900, 1160, 1193
ApoI	RAATTY	1 cut(s)	1079
ArsI	GACNNNNNNTTYG	2 cut(s)	619, 651
Asp700I	GAANNNNTTC	1 cut(s)	285
AspLEI	GCGC	1 cut(s)	1323
AspS9I	GGNCC	2 cut(s)	668, 1247
AsuC2I	CCSGG	1 cut(s)	434
AsuHPI	GGTGA	2 cut(s)	601, 1277
AvaII	GGWCC	2 cut(s)	668, 1247
AxyI	CCTNAGG	1 cut(s)	941
BalI	TGGCCA	1 cut(s)	1195
BanI	GGYRCC	2 cut(s)	1043, 1236
BauI	CACGAG	1 cut(s)	338
BbsI	GAAGAC	2 cut(s)	373, 663
BbvCI	CCTCAGC	1 cut(s)	1311
BccI	CCATC	6 cut(s)	196, 214, 911, 964, 1160, 1285
BceAI	ACGGC	2 cut(s)	883, 997
BciT130I	CCWGG	2 cut(s)	56, 1245
BclI	TGATCA	2 cut(s)	28, 639
BcnI	CCSGG	1 cut(s)	434
BcoDI	GTCTC	2 cut(s)	698, 1022
BfaI	CTAG	3 cut(s)	95, 531, 1176
BfmI	CTRYAG	1 cut(s)	871
BfoI	RGCGCY	1 cut(s)	1324
Bme1390I	CCNGG	3 cut(s)	56, 434, 1245
Bme18I	GGWCC	2 cut(s)	668, 1247
BmgT120I	GGNCC	2 cut(s)	668, 1247
BmiI	GGNNCC	3 cut(s)	303, 1045, 1238
BmrFI	CCNGG	3 cut(s)	56, 434, 1245
BmsI	GCATC	1 cut(s)	352
BpiI	GAAGAC	2 cut(s)	373, 663
BplI	GAGNNNNNCTC	2 cut(s)	26, 58
Bpu10I	CCTNAGC	1 cut(s)	1311
BpuEI	CTTGAG	1 cut(s)	1298
BpuMI	CCSGG	1 cut(s)	434
BsaBI	GATNNNNATC	1 cut(s)	1170
BsaHI	GRCGYC	1 cut(s)	629
BsaI	GGTCTC	2 cut(s)	698, 1022
BsaJI	CCNNGG	3 cut(s)	457, 612, 786
BsaWI	WCCGGW	1 cut(s)	761
BsaXI	ACNNNNNCTCC	2 cut(s)	474, 504
Bsc4I	CCNNNNNNNGG	4 cut(s)	624, 736, 854, 1039
Bse1I	ACTGG	2 cut(s)	685, 1047
Bse21I	CCTNAGG	1 cut(s)	941
Bse3DI	GCAATG	1 cut(s)	1030
Bse8I	GATNNNNATC	1 cut(s)	1170
BseAI	TCCGGA	1 cut(s)	761
BseBI	CCWGG	2 cut(s)	56, 1245
BseDI	CCNNGG	3 cut(s)	457, 612, 786
BseGI	GGATG	4 cut(s)	574, 596, 981, 1171
BseJI	GATNNNNATC	1 cut(s)	1170
BseLI	CCNNNNNNNGG	4 cut(s)	624, 736, 854, 1039
BseMI	GCAATG	1 cut(s)	1030
BseMII	CTCAG	5 cut(s)	390, 452, 828, 1083, 1302
BseNI	ACTGG	2 cut(s)	685, 1047
BseRI	GAGGAG	2 cut(s)	245, 1328
BshFI	GGCC	5 cut(s)	473, 617, 902, 1162, 1195
BshNI	GGYRCC	2 cut(s)	1043, 1236
BsiSI	CCGG	2 cut(s)	434, 762
BslI	CCNNNNNNNGG	4 cut(s)	624, 736, 854, 1039
BsmAI	GTCTC	2 cut(s)	698, 1022
BsmI	GAATGC	1 cut(s)	1347
BsnI	GGCC	5 cut(s)	473, 617, 902, 1162, 1195
Bso31I	GGTCTC	2 cut(s)	698, 1022
Bsp13I	TCCGGA	1 cut(s)	761
Bsp143I	GATC	4 cut(s)	28, 639, 782, 1304
Bsp19I	CCATGG	2 cut(s)	612, 786
BspANI	GGCC	5 cut(s)	473, 617, 902, 1162, 1195
BspCNI	CTCAG	5 cut(s)	389, 451, 827, 1084, 1303
BspEI	TCCGGA	1 cut(s)	761
BspHI	TCATGA	4 cut(s)	25, 31, 226, 384
BspLI	GGNNCC	3 cut(s)	303, 1045, 1238
BspPI	GGATC	1 cut(s)	777
BspT107I	GGYRCC	2 cut(s)	1043, 1236
BspTNI	GGTCTC	2 cut(s)	698, 1022
BsrDI	GCAATG	1 cut(s)	1030
BsrI	ACTGG	2 cut(s)	685, 1047
BssECI	CCNNGG	3 cut(s)	457, 612, 786
BssMI	GATC	4 cut(s)	28, 639, 782, 1304
BssNI	GRCGYC	1 cut(s)	629
BssSI	CACGAG	1 cut(s)	338
BssT1I	CCWWGG	2 cut(s)	612, 786
Bst2BI	CACGAG	1 cut(s)	338
Bst2UI	CCWGG	2 cut(s)	56, 1245
Bst4CI	ACNGT	2 cut(s)	662, 1226
Bst6I	CTCTTC	2 cut(s)	145, 205
BstACI	GRCGYC	1 cut(s)	629
BstC8I	GCNNGC	1 cut(s)	1241
BstDEI	CTNAG	9 cut(s)	164, 376, 438, 814, 835, 941, 1092, 1157, 1311
BstDSI	CCRYGG	2 cut(s)	612, 786
BstF5I	GGATG	4 cut(s)	574, 596, 981, 1171
BstH2I	RGCGCY	1 cut(s)	1324
BstHHI	GCGC	1 cut(s)	1323
BstKTI	GATC	4 cut(s)	31, 642, 785, 1307
BstMAI	GTCTC	2 cut(s)	698, 1022
BstMBI	GATC	4 cut(s)	28, 639, 782, 1304
BstMWI	GCNNNNNNNGC	1 cut(s)	899
BstNI	CCWGG	2 cut(s)	56, 1245
BstNSI	RCATGY	1 cut(s)	327
BstSCI	CCNGG	3 cut(s)	54, 432, 1243
BstSFI	CTRYAG	1 cut(s)	871
BstV2I	GAAGAC	2 cut(s)	373, 663
BstXI	CCANNNNNNTGG	1 cut(s)	362
Bsu36I	CCTNAGG	1 cut(s)	941
BsuRI	GGCC	5 cut(s)	473, 617, 902, 1162, 1195
BtgI	CCRYGG	2 cut(s)	612, 786
BtsCI	GGATG	4 cut(s)	574, 596, 981, 1171
BtsIMutI	CAGTG	2 cut(s)	512, 678
Cac8I	GCNNGC	1 cut(s)	1241
CaiI	CAGNNNCTG	1 cut(s)	514
CciI	TCATGA	4 cut(s)	25, 31, 226, 384
CfoI	GCGC	1 cut(s)	1323
Cfr13I	GGNCC	2 cut(s)	668, 1247
CseI	GACGC	1 cut(s)	637
Csp6I	GTAC	1 cut(s)	937
CviQI	GTAC	1 cut(s)	937
DdeI	CTNAG	9 cut(s)	164, 376, 438, 814, 835, 941, 1092, 1157, 1311
DpnI	GATC	4 cut(s)	30, 641, 784, 1306
DpnII	GATC	4 cut(s)	28, 639, 782, 1304
EaeI	YGGCCR	1 cut(s)	1193
Eam1104I	CTCTTC	2 cut(s)	145, 205
EarI	CTCTTC	2 cut(s)	145, 205
Eco130I	CCWWGG	2 cut(s)	612, 786
Eco31I	GGTCTC	2 cut(s)	698, 1022
Eco47I	GGWCC	2 cut(s)	668, 1247
Eco47III	AGCGCT	1 cut(s)	1322
Eco81I	CCTNAGG	1 cut(s)	941
EcoO109I	RGGNCCY	1 cut(s)	1247
EcoRII	CCWGG	2 cut(s)	54, 1243
EcoT14I	CCWWGG	2 cut(s)	612, 786
EcoT22I	ATGCAT	1 cut(s)	1347
ErhI	CCWWGG	2 cut(s)	612, 786
FalI	AAGNNNNNCTT	3 cut(s)	38, 139, 171
FauNDI	CATATG	1 cut(s)	211
FbaI	TGATCA	2 cut(s)	28, 639
FokI	GGATG	4 cut(s)	581, 583, 988, 1158
FspBI	CTAG	3 cut(s)	95, 531, 1176
GlaI	GCGC	1 cut(s)	1322
HaeII	RGCGCY	1 cut(s)	1324
HaeIII	GGCC	5 cut(s)	473, 617, 902, 1162, 1195
HapII	CCGG	2 cut(s)	434, 762
HgaI	GACGC	1 cut(s)	637
HhaI	GCGC	1 cut(s)	1323
Hin1I	GRCGYC	1 cut(s)	629
Hin6I	GCGC	1 cut(s)	1321
HinP1I	GCGC	1 cut(s)	1321
HinfI	GANTC	5 cut(s)	286, 334, 374, 485, 1066
HpaII	CCGG	2 cut(s)	434, 762
HphI	GGTGA	2 cut(s)	601, 1277
Hpy166II	GTNNAC	1 cut(s)	416
Hpy188I	TCNGA	5 cut(s)	348, 379, 458, 546, 1304
Hpy188III	TCNNGA	8 cut(s)	26, 32, 227, 296, 338, 385, 762, 1329
Hpy8I	GTNNAC	1 cut(s)	416
Hpy99I	CGWCG	2 cut(s)	410, 707
HpyAV	CCTTC	5 cut(s)	484, 533, 1090, 1094, 1105
HpyCH4III	ACNGT	2 cut(s)	662, 1226
HpyCH4IV	ACGT	2 cut(s)	935, 1151
HpyCH4V	TGCA	4 cut(s)	508, 953, 1231, 1345
HpyF10VI	GCNNNNNNNGC	1 cut(s)	899
HpyF3I	CTNAG	9 cut(s)	164, 376, 438, 814, 835, 941, 1092, 1157, 1311
HpySE526I	ACGT	2 cut(s)	935, 1151
Hsp92I	GRCGYC	1 cut(s)	629
HspAI	GCGC	1 cut(s)	1321
Kpn2I	TCCGGA	1 cut(s)	761
Ksp22I	TGATCA	2 cut(s)	28, 639
Kzo9I	GATC	4 cut(s)	28, 639, 782, 1304
LmnI	GCTCC	3 cut(s)	307, 459, 1020
LweI	GCATC	1 cut(s)	352
MaeI	CTAG	3 cut(s)	95, 531, 1176
MaeII	ACGT	2 cut(s)	935, 1151
MaeIII	GTNAC	5 cut(s)	263, 510, 821, 1152, 1207
MalI	GATC	4 cut(s)	30, 641, 784, 1306
MboI	GATC	4 cut(s)	28, 639, 782, 1304
MfeI	CAATTG	1 cut(s)	1034
MlsI	TGGCCA	1 cut(s)	1195
MluCI	AATT	7 cut(s)	84, 449, 919, 994, 1034, 1070, 1079
MluNI	TGGCCA	1 cut(s)	1195
MlyI	GAGTC	1 cut(s)	368
MmeI	TCCRAC	2 cut(s)	460, 695
Mox20I	TGGCCA	1 cut(s)	1195
Mph1103I	ATGCAT	1 cut(s)	1347
MroI	TCCGGA	1 cut(s)	761
MroXI	GAANNNNTTC	1 cut(s)	285
MscI	TGGCCA	1 cut(s)	1195
MseI	TTAA	3 cut(s)	146, 897, 1118
MslI	CAYNNNNRTG	2 cut(s)	30, 517
Msp20I	TGGCCA	1 cut(s)	1195
MspA1I	CMGCKG	1 cut(s)	1310
MspI	CCGG	2 cut(s)	434, 762
MspR9I	CCNGG	3 cut(s)	56, 434, 1245
MunI	CAATTG	1 cut(s)	1034
Mva1269I	GAATGC	1 cut(s)	1347
MvaI	CCWGG	2 cut(s)	56, 1245
MwoI	GCNNNNNNNGC	1 cut(s)	899
NciI	CCSGG	1 cut(s)	434
NcoI	CCATGG	2 cut(s)	612, 786
NdeI	CATATG	1 cut(s)	211
NdeII	GATC	4 cut(s)	28, 639, 782, 1304
NlaIV	GGNNCC	3 cut(s)	303, 1045, 1238
NmuCI	GTSAC	4 cut(s)	263, 510, 821, 1207
NsiI	ATGCAT	1 cut(s)	1347
NspI	RCATGY	1 cut(s)	327
PagI	TCATGA	4 cut(s)	25, 31, 226, 384
PciI	ACATGT	1 cut(s)	323
PcsI	WCGNNNNNNNCGW	1 cut(s)	708
PctI	GAATGC	1 cut(s)	1347
PdmI	GAANNNNTTC	1 cut(s)	285
PfeI	GAWTC	4 cut(s)	286, 334, 485, 1066
PleI	GAGTC	1 cut(s)	368
PpsI	GAGTC	1 cut(s)	368
PpuMI	RGGWCCY	1 cut(s)	1247
PscI	ACATGT	1 cut(s)	323
Psp5II	RGGWCCY	1 cut(s)	1247
Psp6I	CCWGG	2 cut(s)	54, 1243
PspGI	CCWGG	2 cut(s)	54, 1243
PspN4I	GGNNCC	3 cut(s)	303, 1045, 1238
PspPI	GGNCC	2 cut(s)	668, 1247
PspPPI	RGGWCCY	1 cut(s)	1247
PstNI	CAGNNNCTG	1 cut(s)	514
PvuII	CAGCTG	1 cut(s)	1310
RsaI	GTAC	1 cut(s)	938
RsaNI	GTAC	1 cut(s)	937
RseI	CAYNNNNRTG	2 cut(s)	30, 517
SaqAI	TTAA	3 cut(s)	146, 897, 1118
Sau3AI	GATC	4 cut(s)	28, 639, 782, 1304
Sau96I	GGNCC	2 cut(s)	668, 1247
SchI	GAGTC	1 cut(s)	368
ScrFI	CCNGG	3 cut(s)	56, 434, 1245
SfaNI	GCATC	1 cut(s)	352
SfcI	CTRYAG	1 cut(s)	871
SinI	GGWCC	2 cut(s)	668, 1247
SmiMI	CAYNNNNRTG	2 cut(s)	30, 517
SmlI	CTYRAG	1 cut(s)	1277
SmoI	CTYRAG	1 cut(s)	1277
Sse9I	AATT	7 cut(s)	84, 449, 919, 994, 1034, 1070, 1079
SspMI	CTAG	3 cut(s)	95, 531, 1176
StyD4I	CCNGG	3 cut(s)	54, 432, 1243
StyI	CCWWGG	2 cut(s)	612, 786
TaaI	ACNGT	2 cut(s)	662, 1226
TaiI	ACGT	2 cut(s)	938, 1154
TaqI	TCGA	2 cut(s)	702, 711
TaqII	GACCGA	1 cut(s)	680
TasI	AATT	7 cut(s)	84, 449, 919, 994, 1034, 1070, 1079
TfiI	GAWTC	4 cut(s)	286, 334, 485, 1066
Tru1I	TTAA	3 cut(s)	146, 897, 1118
Tru9I	TTAA	3 cut(s)	146, 897, 1118
TscAI	CASTG	2 cut(s)	519, 685
TseFI	GTSAC	4 cut(s)	263, 510, 821, 1207
Tsp45I	GTSAC	4 cut(s)	263, 510, 821, 1207
TspDTI	ATGAA	9 cut(s)	14, 17, 132, 231, 373, 401, 546, 766, 813
TspRI	CASTG	2 cut(s)	519, 685
VpaK11BI	GGWCC	2 cut(s)	668, 1247
XapI	RAATTY	1 cut(s)	1079
XceI	RCATGY	1 cut(s)	327
XmnI	GAANNNNTTC	1 cut(s)	285
XspI	CTAG	3 cut(s)	95, 531, 1176
Zsp2I	ATGCAT	1 cut(s)	1347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.