Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0001626

Physical Location & Seq

Genomic Coordinates Forward (+)

4666828 .. 4673786

Gene Structure 6959 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0062800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2028 bp

ATGGCTAGCATGGCTCGGGCGAGCTCGGGGCTCCAATATCCTGAGCGGTTCTACGCGGCGGCCTCCTACGCCGGCTTCGACGGATCTCCTCGCTCCTCTTCTAAAGCCGTTCGCGCCAAGTTCAACTCCGAGTCCGCTCTCATCCTCTACGCTCTCTACCAGCAGGCTACTGTAGGACCGTGTAACACACCAGAACCGAGTGCTTGGAATGCAGTTGAGAAAAGCAAATGGAGCAGCTGGAAGAAGCTTGCGGACATGGCCTCCACTGAAGCAATGCGTCTTTTTGTGAAGATATTGGAGGAAGAAGATCCAGGTTGGTATTCAAGAGCATCGAACTCTGTTTCTGAGCCTGTGGTAGATGTACAAATGAATCAAAATTCAAAAGTTGGGCCAGTTGTTGAGAATGGGAACTCTTATCCAAAGACAAAGACAATCTCCGCTGAAAATGGGAGCATACAGGAAGCACAGGATAAAGATGTTGTCTCCGAAGGCATTGGTTCTGTTGGTGTTTACGATCAGTGGATTGCACCTCCAGTATCTGGCCAACGTCCGAAAGCCCGATATGAGCATGGAGCAGCAGTTATAAAAGACAAGATGTATATATATGGTGGAAACCACAATGGTCGTTACCTAAATGATCTCCATGTCTTCGATTTAAGGAGTTGGACTTGGTCAAAGATTGAGGCCAAAGTTGGGGCTGAATCACACGACTCGCCGTCTCCAGTAACAATAAGCCCATGTGCTGGTCATTCCTTGATACCATGGGGCAATAAACTTATCTCAATTGCTGGACATACAAAGGATCCTACGGAGTCTATTCAAGTAAAGGTGTTTGATCTACACACTTCTACTTGGTCGTTCTTAAAGACTTATGGGAAACCACCGACATCACGCGGAGGTCAATCCGTCACCCTTATTGGAGGTAGCTTGGTGATTTTTGGTGGACAGGATGCGAAGAGAACTTTGTTAAATGATCTCCACATTCTTGACCTAGAAACAATGACATGGGATGAGATTGATGCTGTAGGGGTGTCCCCTTCTCCAAGGTCTGATCATGCTGCTGCTGTTCATGCTGACCGCTACCTCCTTATCTTTGGTGGGGGTTCCCATGCAACTTGCTTCAATGATCTGCATGTCCTTGATTTGCAAACTATGGAGTGGTCACGACCCACACAACAAGGCGAGATACCAACTCCACGGGCTGGACATGCAGGTGTGCCAGTTGGGGAAAATTGGTTCATTGTTGGTGGTGGTGACAATAAGAGTGGGGTTTCAGAAACCGTTGTCCTAAATATGTCTACACTAGTTTGGTCTGTTGTAACTACTGTTGAAGGGCGTATTCCTGTTGCTAGTGAGGGATTAAGTTTGGTCGTAGGTTCTTACAATGGTGAAGATGTGCTTTTATCTTTTGGAGGATACAATGGGCGTTACAACAATGAGGTTAACGTTCTCAAACCAAGCCACAAGTCAAGCTTGCAATCAAAAATGGAAACTTCTGTCCCTGACAGTGTTTCTGCCGTGCATAATGCCACTACTGCTACCAGAGATGTGGAGTCCGAGTATGGAGCTGGACAAGAAGGAAAAATTAGGGAAATAGTTATGGACAATGTTGACACAGAGGCAATGAAATTCAGAGGCAAGAATTTCAATGAGCATCTTATAGCATCGCTCAAAGGAGATAAAGAGGAATTAGAATCATCACTTAGTAAGGAGAAGGCGCAATCTCTCCAATTAAAACAAGAGTTATCAGAAGCAGAGACTCGTAACACTGACCTATACAAGGAGCTTCAATCTGTACGGGGACAACTTGCATCTGAGCAGTCCAGATGCTTCAAACTTGAGGTTGAACTAGCAGAACTACGACAGAAGCTCCAAACAATGGAGGCATTGCAGAAGGAACTTGAACTCCTCCAGCGACAAAAAGCTGCATCTGAACAAGCTGCTCTGAATGCTAAAAAGCAGGGCTCAGGAGGCGTCTGGGGCTGGCTTGCCGGAACACCTGGCAGTGAAAAATCTGATGATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003700 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006333 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030527 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03410 ko04140 ko04217 map03410 map04140 map04217

Pfam Domains

Protein Families

HMG_box

Protein Analysis

675

Amino Acids

73.48

Weight (kDa)

5.63

Isoelectric Point (pI)

43.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACBP	PF00887	17 - 98	9.5e-19	Acyl CoA binding protein
Kelch_HCF	PF13854	171 - 367	2.5e-21	Host cell factor, Kelch-repeats domain
Beta-prop_FBX42	PF13415	172 - 230	2.9e-12	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	172 - 303	4.3e-19	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	173 - 230	9.8e-17	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4	PF13418	183 - 374	2e-06	tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1	PF01344	186 - 227	3.6e-10	Kelch motif
Beta-prop_FBX42	PF13415	245 - 369	1.3e-24	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	249 - 472	3.8e-57	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	262 - 394	1.1e-17	Attractin/LZTR1 beta-propeller
Beta-prop_ATRN-LZTR1	PF24981	311 - 494	4.9e-21	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	352 - 503	1e-21	FBX42, beta-propeller domain
Kelch_HCF	PF13854	361 - 417	3.6e-07	Host cell factor, Kelch-repeats domain
Kelch_HCF	PF13854	393 - 488	7.6e-06	Host cell factor, Kelch-repeats domain
ACBP4_C	PF24922	551 - 654	2.1e-40	Acyl-CoA-binding domain-containing protein 4-6, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001626)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51880 AT3G51880 AT3G51880 AT3G51880 AT3G51880
fragaria_vesca	FvH4_6g11530 FvH4_6g11530 FvH4_6g11530 FvH4_6g11530 FvH4_7g24360 FvH4_7g24360 FvH4_7g24360
malus_domestica	MD01G1132900.v1.1 MD07G1220600.v1.1
prunus_persica	Prupe.2G249000_v2.0.a1 Prupe.2G249000_v2.0.a1 Prupe.6G276300_v2.0.a1 Prupe.6G276300_v2.0.a1 Prupe.6G276300_v2.0.a1 Prupe.6G276300_v2.0.a1
pyrus_communis	pycom01g16950 pycom07g19960
rosa_chinensis	RchiOBHm_Chr1g0370021 RchiOBHm_Chr3g0463231
rosa_laevigata	RLG00000024799
rosa_multiflora	Rmu_co8427781.1_g000001 Rmu_sc0005056.1_g000014 Rmu_sc0008079.1_g000019 Rmu_sc0022743.1_g000005 Rmu_sc0022743.1_g000006
rosa_roxburghii	Rroxscaffold_4G00286860 Rroxscaffold_4G00287170 Rroxscaffold_6G00416770
rosa_rugosa	Rorug01G0353500 Rorug03G0062700 Rorug03G0062700 Rorug03G0062700 Rorug03G0062800 Rorug03G0062800
rosa_samantha	Rh1AG362200 Rh1BG325400 Rh1CG338700 Rh1DG355900 Rh3AG120800 Rh3BG125300 Rh3CG127300 Rh3DG126500
rosa_wichuraiana	Rw1G031870 Rw3G010260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	584
AarI	CACCTGC	1 cut(s)	1202
Acc36I	ACCTGC	1 cut(s)	1202
AccB7I	CCANNNNNTGG	4 cut(s)	539, 743, 1202, 1879
AccBSI	CCGCTC	2 cut(s)	46, 137
AccI	GTMKAC	1 cut(s)	1298
AccII	CGCG	3 cut(s)	56, 114, 894
AciI	CCGC	8 cut(s)	46, 56, 59, 135, 251, 438, 894, 1078
AclI	AACGTT	1 cut(s)	1446
AclWI	GGATC	4 cut(s)	91, 302, 797, 810
AcoI	YGGCCR	1 cut(s)	541
AcsI	RAATTY	3 cut(s)	376, 1628, 1642
AcuI	CTGAAG	1 cut(s)	288
AcyI	GRCGYC	1 cut(s)	1974
AfaI	GTAC	2 cut(s)	363, 1797
AfiI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1780, 1879
AhdI	GACNNNNNGTC	1 cut(s)	715
AhlI	ACTAGT	1 cut(s)	1303
AjnI	CCWGG	2 cut(s)	310, 1999
AloI	GAACNNNNNNTCC	4 cut(s)	1220, 1252, 1890, 1922
Alw21I	GWGCWC	1 cut(s)	26
Alw26I	GTCTC	3 cut(s)	487, 723, 1751
AlwI	GGATC	4 cut(s)	91, 302, 797, 810
AlwNI	CAGNNNCTG	1 cut(s)	539
Ama87I	CYCGRG	2 cut(s)	15, 25
AoxI	GGCC	5 cut(s)	60, 258, 389, 541, 684
ApeKI	GCWGC	6 cut(s)	234, 575, 1058, 1061, 1925, 1940
ApoI	RAATTY	3 cut(s)	376, 1628, 1642
AspLEI	GCGC	2 cut(s)	116, 1720
AspS9I	GGNCC	2 cut(s)	176, 389
AsuHPI	GGTGA	4 cut(s)	901, 943, 1265, 1400
AsuNHI	GCTAGC	1 cut(s)	5
AvaI	CYCGRG	2 cut(s)	15, 25
AvaII	GGWCC	1 cut(s)	176
BalI	TGGCCA	1 cut(s)	543
BamHI	GGATCC	1 cut(s)	802
BanII	GRGCYC	3 cut(s)	26, 33, 1967
BbsI	GAAGAC	1 cut(s)	640
Bbv12I	GWGCWC	1 cut(s)	26
BbvI	GCAGC	6 cut(s)	246, 587, 1045, 1048, 1912, 1927
BceAI	ACGGC	3 cut(s)	92, 700, 1502
BciT130I	CCWGG	2 cut(s)	312, 2001
BciVI	GTATCC	1 cut(s)	1409
BclI	TGATCA	1 cut(s)	1051
BcoDI	GTCTC	3 cut(s)	487, 723, 1751
BcuI	ACTAGT	1 cut(s)	1303
BfaI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1850
BfmI	CTRYAG	2 cut(s)	171, 1023
BfuAI	ACCTGC	1 cut(s)	1202
BfuI	GTATCC	1 cut(s)	1409
BisI	GCNGC	8 cut(s)	57, 60, 235, 576, 1059, 1062, 1926, 1941
BlsI	GCNGC	8 cut(s)	58, 61, 236, 577, 1060, 1063, 1927, 1942
Bme1390I	CCNGG	2 cut(s)	312, 2001
Bme18I	GGWCC	1 cut(s)	176
BmeRI	GACNNNNNGTC	1 cut(s)	715
BmeT110I	CYCGRG	2 cut(s)	15, 25
BmgT120I	GGNCC	2 cut(s)	176, 389
BmiI	GGNNCC	3 cut(s)	32, 804, 1105
BmrFI	CCNGG	2 cut(s)	312, 2001
BmsI	GCATC	9 cut(s)	338, 940, 1009, 1663, 1673, 1817, 1820, 1937, 2011
BmtI	GCTAGC	1 cut(s)	9
BpiI	GAAGAC	1 cut(s)	640
BpmI	CTGGAG	3 cut(s)	516, 705, 1895
Bpu10I	CCTNAGC	2 cut(s)	42, 1966
BpuEI	CTTGAG	1 cut(s)	1859
BsaHI	GRCGYC	1 cut(s)	1974
BsaJI	CCNNGG	3 cut(s)	761, 1043, 1196
Bsc4I	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1780, 1879
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	4 cut(s)	392, 533, 722, 1220
Bse3DI	GCAATG	3 cut(s)	279, 1629, 1886
BseBI	CCWGG	2 cut(s)	312, 2001
BseDI	CCNNGG	3 cut(s)	761, 1043, 1196
BseGI	GGATG	3 cut(s)	141, 955, 1015
BseLI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1780, 1879
BseMI	GCAATG	3 cut(s)	279, 1629, 1886
BseMII	CTCAG	4 cut(s)	33, 336, 1806, 1980
BseNI	ACTGG	4 cut(s)	392, 533, 722, 1220
BseRI	GAGGAG	3 cut(s)	78, 85, 1898
BseXI	GCAGC	6 cut(s)	246, 587, 1045, 1048, 1912, 1927
Bsh1236I	CGCG	3 cut(s)	56, 114, 894
BshFI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BsiHKAI	GWGCWC	1 cut(s)	26
BsiHKCI	CYCGRG	2 cut(s)	15, 25
BsiSI	CCGG	2 cut(s)	72, 1992
BslFI	GGGAC	3 cut(s)	1018, 1484, 1815
BslI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1780, 1879
BsmAI	GTCTC	3 cut(s)	487, 723, 1751
BsmBI	CGTCTC	1 cut(s)	723
BsmFI	GGGAC	3 cut(s)	1018, 1484, 1815
BsmI	GAATGC	2 cut(s)	214, 1954
BsnI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BsoBI	CYCGRG	2 cut(s)	15, 25
Bsp1286I	GDGCHC	3 cut(s)	26, 33, 1967
Bsp1407I	TGTACA	1 cut(s)	361
Bsp143I	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
Bsp19I	CCATGG	1 cut(s)	761
BspACI	CCGC	8 cut(s)	46, 56, 59, 135, 251, 438, 894, 1078
BspANI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BspCNI	CTCAG	4 cut(s)	34, 337, 1807, 1979
BspFNI	CGCG	3 cut(s)	56, 114, 894
BspLI	GGNNCC	3 cut(s)	32, 804, 1105
BspMI	ACCTGC	1 cut(s)	1202
BspOI	GCTAGC	1 cut(s)	9
BspPI	GGATC	4 cut(s)	91, 302, 797, 810
BsrBI	CCGCTC	2 cut(s)	46, 137
BsrDI	GCAATG	3 cut(s)	279, 1629, 1886
BsrFI	RCCGGY	1 cut(s)	71
BsrGI	TGTACA	1 cut(s)	361
BsrI	ACTGG	4 cut(s)	392, 533, 722, 1220
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	3 cut(s)	761, 1043, 1196
BssMI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
BssNI	GRCGYC	1 cut(s)	1974
BssT1I	CCWWGG	2 cut(s)	761, 1043
Bst2UI	CCWGG	2 cut(s)	312, 2001
Bst4CI	ACNGT	5 cut(s)	172, 180, 1282, 1327, 1508
Bst6I	CTCTTC	2 cut(s)	103, 950
BstACI	GRCGYC	1 cut(s)	1974
BstAUI	TGTACA	1 cut(s)	361
BstC8I	GCNNGC	8 cut(s)	7, 22, 73, 165, 249, 1475, 1985, 1989
BstDEI	CTNAG	5 cut(s)	42, 345, 1703, 1815, 1966
BstDSI	CCRYGG	2 cut(s)	761, 1196
BstENI	CCTNNNNNAGG	1 cut(s)	1778
BstF5I	GGATG	3 cut(s)	141, 955, 1015
BstFNI	CGCG	3 cut(s)	56, 114, 894
BstHHI	GCGC	2 cut(s)	116, 1720
BstKTI	GATC	9 cut(s)	86, 310, 517, 640, 805, 838, 976, 1054, 1129
BstMAI	GTCTC	3 cut(s)	487, 723, 1751
BstMBI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
BstNI	CCWGG	2 cut(s)	312, 2001
BstNSI	RCATGY	2 cut(s)	1136, 1211
BstSCI	CCNGG	2 cut(s)	310, 1999
BstSFI	CTRYAG	2 cut(s)	171, 1023
BstUI	CGCG	3 cut(s)	56, 114, 894
BstV1I	GCAGC	6 cut(s)	246, 587, 1045, 1048, 1912, 1927
BstV2I	GAAGAC	1 cut(s)	640
BstX2I	RGATCY	3 cut(s)	83, 307, 802
BstXI	CCANNNNNNTGG	1 cut(s)	1549
BstYI	RGATCY	3 cut(s)	83, 307, 802
BsuI	GTATCC	1 cut(s)	1409
BsuRI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BtgI	CCRYGG	2 cut(s)	761, 1196
BtgZI	GCGATG	1 cut(s)	1650
BtsCI	GGATG	3 cut(s)	141, 955, 1015
BtsI	GCAGTG	1 cut(s)	2011
BtsIMutI	CAGTG	5 cut(s)	264, 524, 1513, 1767, 2011
BveI	ACCTGC	1 cut(s)	1202
Cac8I	GCNNGC	8 cut(s)	7, 22, 73, 165, 249, 1475, 1985, 1989
CaiI	CAGNNNCTG	1 cut(s)	539
CfoI	GCGC	2 cut(s)	116, 1720
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	2 cut(s)	176, 389
CseI	GACGC	2 cut(s)	266, 1963
Csp6I	GTAC	2 cut(s)	362, 1796
CspCI	CAANNNNNGTGG	2 cut(s)	1246, 1281
CviQI	GTAC	2 cut(s)	362, 1796
DdeI	CTNAG	5 cut(s)	42, 345, 1703, 1815, 1966
DpnI	GATC	9 cut(s)	85, 309, 516, 639, 804, 837, 975, 1053, 1128
DpnII	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
DriI	GACNNNNNGTC	1 cut(s)	715
EaeI	YGGCCR	1 cut(s)	541
Eam1104I	CTCTTC	2 cut(s)	103, 950
Eam1105I	GACNNNNNGTC	1 cut(s)	715
EarI	CTCTTC	2 cut(s)	103, 950
Ecl136II	GAGCTC	1 cut(s)	24
Eco130I	CCWWGG	2 cut(s)	761, 1043
Eco24I	GRGCYC	3 cut(s)	26, 33, 1967
Eco47I	GGWCC	1 cut(s)	176
Eco53kI	GAGCTC	1 cut(s)	24
Eco57I	CTGAAG	1 cut(s)	288
Eco88I	CYCGRG	2 cut(s)	15, 25
EcoICRI	GAGCTC	1 cut(s)	24
EcoNI	CCTNNNNNAGG	1 cut(s)	1778
EcoRII	CCWGG	2 cut(s)	310, 1999
EcoT14I	CCWWGG	2 cut(s)	761, 1043
EcoT38I	GRGCYC	3 cut(s)	26, 33, 1967
ErhI	CCWWGG	2 cut(s)	761, 1043
Esp3I	CGTCTC	1 cut(s)	723
FalI	AAGNNNNNCTT	4 cut(s)	1383, 1415, 1457, 1489
FaqI	GGGAC	3 cut(s)	1018, 1484, 1815
FbaI	TGATCA	1 cut(s)	1051
FblI	GTMKAC	1 cut(s)	1298
Fnu4HI	GCNGC	8 cut(s)	57, 60, 235, 576, 1059, 1062, 1926, 1941
FokI	GGATG	3 cut(s)	128, 962, 1022
FriOI	GRGCYC	3 cut(s)	26, 33, 1967
Fsp4HI	GCNGC	8 cut(s)	57, 60, 235, 576, 1059, 1062, 1926, 1941
FspBI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1850
GlaI	GCGC	2 cut(s)	115, 1719
GluI	GCNGC	8 cut(s)	57, 60, 235, 576, 1059, 1062, 1926, 1941
GsuI	CTGGAG	3 cut(s)	516, 705, 1895
HaeIII	GGCC	5 cut(s)	62, 260, 391, 543, 686
HapII	CCGG	2 cut(s)	72, 1992
HgaI	GACGC	2 cut(s)	266, 1963
HhaI	GCGC	2 cut(s)	116, 1720
Hin1I	GRCGYC	1 cut(s)	1974
Hin6I	GCGC	2 cut(s)	114, 1718
HinP1I	GCGC	2 cut(s)	114, 1718
HincII	GTYRAC	2 cut(s)	1444, 1612
HindII	GTYRAC	2 cut(s)	1444, 1612
HindIII	AAGCTT	2 cut(s)	245, 1471
HinfI	GANTC	8 cut(s)	131, 370, 701, 710, 812, 1553, 1694, 1759
HpaI	GTTAAC	1 cut(s)	1444
HpaII	CCGG	2 cut(s)	72, 1992
HphI	GGTGA	4 cut(s)	901, 943, 1265, 1400
Hpy166II	GTNNAC	5 cut(s)	511, 944, 1299, 1444, 1612
Hpy188III	TCNNGA	6 cut(s)	41, 324, 986, 1164, 1824, 1968
Hpy8I	GTNNAC	5 cut(s)	511, 944, 1299, 1444, 1612
Hpy99I	CGWCG	1 cut(s)	83
HpyAV	CCTTC	6 cut(s)	482, 1047, 1325, 1571, 1708, 1888
HpyCH4III	ACNGT	5 cut(s)	172, 180, 1282, 1327, 1508
HpyCH4IV	ACGT	2 cut(s)	547, 1446
HpyF3I	CTNAG	5 cut(s)	42, 345, 1703, 1815, 1966
HpySE526I	ACGT	2 cut(s)	547, 1446
Hsp92I	GRCGYC	1 cut(s)	1974
HspAI	GCGC	2 cut(s)	114, 1718
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
Ksp22I	TGATCA	1 cut(s)	1051
KspAI	GTTAAC	1 cut(s)	1444
Kzo9I	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
LmnI	GCTCC	8 cut(s)	36, 98, 231, 450, 572, 1565, 1783, 1875
Lsp1109I	GCAGC	6 cut(s)	246, 587, 1045, 1048, 1912, 1927
LweI	GCATC	9 cut(s)	338, 940, 1009, 1663, 1673, 1817, 1820, 1937, 2011
MaeI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1850
MaeII	ACGT	2 cut(s)	547, 1446
MaeIII	GTNAC	9 cut(s)	182, 626, 724, 907, 1161, 1253, 1318, 1427, 1763
MalI	GATC	9 cut(s)	85, 309, 516, 639, 804, 837, 975, 1053, 1128
MbiI	CCGCTC	2 cut(s)	46, 137
MboI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
MboII	GAAGA	8 cut(s)	90, 253, 301, 314, 317, 640, 967, 1403
MfeI	CAATTG	1 cut(s)	783
MflI	RGATCY	3 cut(s)	83, 307, 802
MhlI	GDGCHC	3 cut(s)	26, 33, 1967
MlsI	TGGCCA	1 cut(s)	543
MluCI	AATT	8 cut(s)	376, 783, 1231, 1584, 1628, 1642, 1688, 1730
MluNI	TGGCCA	1 cut(s)	543
MlyI	GAGTC	5 cut(s)	140, 704, 821, 1562, 1753
MmeI	TCCRAC	1 cut(s)	644
Mox20I	TGGCCA	1 cut(s)	543
MroNI	GCCGGC	1 cut(s)	71
MscI	TGGCCA	1 cut(s)	543
MseI	TTAA	6 cut(s)	656, 863, 968, 1361, 1443, 1733
MslI	CAYNNNNRTG	1 cut(s)	1212
Msp20I	TGGCCA	1 cut(s)	543
MspA1I	CMGCKG	2 cut(s)	237, 440
MspI	CCGG	2 cut(s)	72, 1992
MspR9I	CCNGG	2 cut(s)	312, 2001
MunI	CAATTG	1 cut(s)	783
Mva1269I	GAATGC	2 cut(s)	214, 1954
MvaI	CCWGG	2 cut(s)	312, 2001
MvnI	CGCG	3 cut(s)	56, 114, 894
NaeI	GCCGGC	1 cut(s)	73
NcoI	CCATGG	1 cut(s)	761
NdeII	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
NgoMIV	GCCGGC	1 cut(s)	71
NheI	GCTAGC	1 cut(s)	5
NlaIV	GGNNCC	3 cut(s)	32, 804, 1105
NmuCI	GTSAC	3 cut(s)	907, 1161, 1253
NspI	RCATGY	2 cut(s)	1136, 1211
PaqCI	CACCTGC	1 cut(s)	1202
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	2 cut(s)	214, 1954
PdiI	GCCGGC	1 cut(s)	73
PfeI	GAWTC	3 cut(s)	370, 701, 1694
PflFI	GACNNNGTC	1 cut(s)	670
PflMI	CCANNNNNTGG	4 cut(s)	539, 743, 1202, 1879
PkrI	GCNGC	8 cut(s)	58, 61, 236, 577, 1060, 1063, 1927, 1942
PleI	GAGTC	5 cut(s)	139, 704, 820, 1561, 1753
PpsI	GAGTC	5 cut(s)	139, 704, 820, 1561, 1753
PsiI	TTATAA	1 cut(s)	584
Psp124BI	GAGCTC	1 cut(s)	26
Psp1406I	AACGTT	1 cut(s)	1446
Psp6I	CCWGG	2 cut(s)	310, 1999
PspGI	CCWGG	2 cut(s)	310, 1999
PspN4I	GGNNCC	3 cut(s)	32, 804, 1105
PspPI	GGNCC	2 cut(s)	176, 389
PstNI	CAGNNNCTG	1 cut(s)	539
PsuI	RGATCY	3 cut(s)	83, 307, 802
PsyI	GACNNNGTC	1 cut(s)	670
PvuII	CAGCTG	1 cut(s)	237
RsaI	GTAC	2 cut(s)	363, 1797
RsaNI	GTAC	2 cut(s)	362, 1796
RseI	CAYNNNNRTG	1 cut(s)	1212
SacI	GAGCTC	1 cut(s)	26
SaqAI	TTAA	6 cut(s)	656, 863, 968, 1361, 1443, 1733
SatI	GCNGC	8 cut(s)	57, 60, 235, 576, 1059, 1062, 1926, 1941
Sau3AI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
Sau96I	GGNCC	2 cut(s)	176, 389
SchI	GAGTC	5 cut(s)	140, 704, 821, 1562, 1753
ScrFI	CCNGG	2 cut(s)	312, 2001
SduI	GDGCHC	3 cut(s)	26, 33, 1967
SfaNI	GCATC	9 cut(s)	338, 940, 1009, 1663, 1673, 1817, 1820, 1937, 2011
SfcI	CTRYAG	2 cut(s)	171, 1023
SinI	GGWCC	1 cut(s)	176
SmiMI	CAYNNNNRTG	1 cut(s)	1212
SmlI	CTYRAG	1 cut(s)	1838
SmoI	CTYRAG	1 cut(s)	1838
SpeI	ACTAGT	1 cut(s)	1303
Sse9I	AATT	8 cut(s)	376, 783, 1231, 1584, 1628, 1642, 1688, 1730
SsiI	CCGC	8 cut(s)	46, 56, 59, 135, 251, 438, 894, 1078
SspMI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1850
SstI	GAGCTC	1 cut(s)	26
StyD4I	CCNGG	2 cut(s)	310, 1999
StyI	CCWWGG	2 cut(s)	761, 1043
TaaI	ACNGT	5 cut(s)	172, 180, 1282, 1327, 1508
TaiI	ACGT	2 cut(s)	550, 1449
TaqI	TCGA	3 cut(s)	78, 332, 651
TasI	AATT	8 cut(s)	376, 783, 1231, 1584, 1628, 1642, 1688, 1730
TatI	WGTACW	1 cut(s)	361
TauI	GCSGC	2 cut(s)	59, 62
TfiI	GAWTC	3 cut(s)	370, 701, 1694
Tru1I	TTAA	6 cut(s)	656, 863, 968, 1361, 1443, 1733
Tru9I	TTAA	6 cut(s)	656, 863, 968, 1361, 1443, 1733
TscAI	CASTG	5 cut(s)	271, 524, 1513, 1774, 2011
TseFI	GTSAC	3 cut(s)	907, 1161, 1253
TseI	GCWGC	6 cut(s)	234, 575, 1058, 1061, 1925, 1940
Tsp45I	GTSAC	3 cut(s)	907, 1161, 1253
TspDTI	ATGAA	4 cut(s)	383, 1058, 1228, 1640
TspGWI	ACGGA	3 cut(s)	96, 824, 895
TspRI	CASTG	5 cut(s)	271, 524, 1513, 1774, 2011
Tth111I	GACNNNGTC	1 cut(s)	670
Van91I	CCANNNNNTGG	4 cut(s)	539, 743, 1202, 1879
VpaK11BI	GGWCC	1 cut(s)	176
XagI	CCTNNNNNAGG	1 cut(s)	1778
XapI	RAATTY	3 cut(s)	376, 1628, 1642
XceI	RCATGY	2 cut(s)	1136, 1211
XmiI	GTMKAC	1 cut(s)	1298
XspI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.