Rorug03G0285100

ubiquitin protein ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006692
Physical Location & Seq
Reverse (-)
28659023 .. 28660602
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0285100.1

Sequence Viewer

Length: 453 bp
ATGATCAAATCCAGCGACGGGTCGTCCACCAAATCAAATGTCTTTGCACAAAAACGGCGGCACCGGAGTCTCCTGCAGACACTGAAGCGGCTGCGGCACTCCAATTTGGGATATGAAAGTTTGGCAGAGAGTGAACACTGCAAACTCTTGAAAGGTCGCCCATTTCAGTTTCAGGATCCTAATTCAAAGCATCTAGGAACTATGGTTTGGGATGCATCACTGGTATTCGCCAAGTTTCTGATTGGGGATCCTGTTTGGATTGACAGAGCTATGTATGCGCTGAAGAAGTCGGATGGGATTATGGAGGAGGTGACCGAGGAGGAGTTGATGGATGCCCAAGTGGATTCTACTGGCATGTTTATATTTCCTCACACTGGTGTGGTGCTGGCGGCGTTGATTAAGCTCCAGATTTGGCCTGCCGGTTTGCCAACCCACCGGTTCAAGTGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

17.04

Weight (kDa)

9.3

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 60
AciI CCGC 4 cut(s) 58, 88, 94, 389
AclWI GGATC 4 cut(s) 170, 183, 242, 255
AcuI CTGAAG 2 cut(s) 104, 302
AfiI CCNNNNNNNGG 2 cut(s) 18, 374
AgeI ACCGGT 1 cut(s) 435
AgsI TTSAA 3 cut(s) 151, 186, 442
AhdI GACNNNNNGTC 1 cut(s) 22
AjuI GAANNNNNNNTTGG 2 cut(s) 190, 222
AleI CACNNNNGTG 2 cut(s) 375, 377
AluBI AGCT 2 cut(s) 269, 403
AluI AGCT 2 cut(s) 269, 403
Alw26I GTCTC 1 cut(s) 74
AlwI GGATC 4 cut(s) 170, 183, 242, 255
AlwNI CAGNNNCTG 1 cut(s) 82
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 91
ArsI GACNNNNNNTTYG 2 cut(s) 24, 56
AsiGI ACCGGT 1 cut(s) 435
AspLEI GCGC 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 322
BamHI GGATCC 2 cut(s) 175, 247
BanI GGYRCC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 78
BccI CCATC 2 cut(s) 287, 322
BceAI ACGGC 1 cut(s) 71
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 5 cut(s) 59, 89, 92, 95, 390
BlsI GCNGC 5 cut(s) 60, 90, 93, 96, 391
BmeRI GACNNNNNGTC 1 cut(s) 22
BmiI GGNNCC 3 cut(s) 62, 177, 249
BmsI GCATC 4 cut(s) 199, 202, 224, 322
BpmI CTGGAG 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 2 cut(s) 63, 435
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 374
Bse118I RCCGGY 2 cut(s) 419, 435
Bse1I ACTGG 3 cut(s) 225, 355, 379
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 217, 298, 337
BseLI CCNNNNNNNGG 2 cut(s) 18, 374
BseNI ACTGG 3 cut(s) 225, 355, 379
BseRI GAGGAG 3 cut(s) 320, 332, 335
BseXI GCAGC 1 cut(s) 78
BshFI GGCC 1 cut(s) 415
BshNI GGYRCC 1 cut(s) 60
BshTI ACCGGT 1 cut(s) 435
BsiSI CCGG 3 cut(s) 64, 420, 436
BslI CCNNNNNNNGG 2 cut(s) 18, 374
BsmAI GTCTC 1 cut(s) 74
BsnI GGCC 1 cut(s) 415
Bsp143I GATC 3 cut(s) 3, 175, 247
BspACI CCGC 4 cut(s) 58, 88, 94, 389
BspANI GGCC 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 62, 177, 249
BspMAI CTGCAG 1 cut(s) 78
BspPI GGATC 4 cut(s) 170, 183, 242, 255
BspT107I GGYRCC 1 cut(s) 60
BsrFI RCCGGY 2 cut(s) 419, 435
BsrI ACTGG 3 cut(s) 225, 355, 379
BssAI RCCGGY 2 cut(s) 419, 435
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 3, 175, 247
BstC8I GCNNGC 2 cut(s) 387, 417
BstEII GGTNACC 1 cut(s) 310
BstF5I GGATG 3 cut(s) 217, 298, 337
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 3 cut(s) 6, 178, 250
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 3 cut(s) 3, 175, 247
BstMWI GCNNNNNNNGC 2 cut(s) 94, 275
BstNSI RCATGY 1 cut(s) 358
BstPI GGTNACC 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 175, 247
BstYI RGATCY 2 cut(s) 175, 247
BsuRI GGCC 1 cut(s) 415
BtsCI GGATG 3 cut(s) 217, 298, 337
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 4 cut(s) 80, 136, 218, 372
Cac8I GCNNGC 2 cut(s) 387, 417
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 2 cut(s) 419, 435
CspAI ACCGGT 1 cut(s) 435
CviAII CATG 1 cut(s) 355
CviJI RGCY 5 cut(s) 91, 269, 403, 415, 450
CviKI_1 RGCY 5 cut(s) 91, 269, 403, 415, 450
DpnI GATC 3 cut(s) 5, 177, 249
DpnII GATC 3 cut(s) 3, 175, 247
DriI GACNNNNNGTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 104, 302
Eco91I GGTNACC 1 cut(s) 310
EcoO65I GGTNACC 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 217
FaeI CATG 1 cut(s) 358
FaiI YATR 7 cut(s) 114, 203, 272, 276, 302, 356, 362
FatI CATG 1 cut(s) 354
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 5 cut(s) 59, 89, 92, 95, 390
FokI GGATG 3 cut(s) 224, 305, 344
Fsp4HI GCNGC 5 cut(s) 59, 89, 92, 95, 390
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 279
GluI GCNGC 5 cut(s) 59, 89, 92, 95, 390
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 1 cut(s) 415
HapII CCGG 3 cut(s) 64, 420, 436
HhaI GCGC 1 cut(s) 280
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HinfI GANTC 2 cut(s) 67, 344
HpaII CCGG 3 cut(s) 64, 420, 436
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 2 cut(s) 27, 134
Hpy188I TCNGA 2 cut(s) 240, 292
Hpy188III TCNNGA 3 cut(s) 148, 173, 406
Hpy8I GTNNAC 2 cut(s) 27, 134
Hpy99I CGWCG 1 cut(s) 20
HpyCH4V TGCA 4 cut(s) 47, 76, 141, 215
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 275
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 175, 247
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 1 cut(s) 78
LweI GCATC 4 cut(s) 199, 202, 224, 322
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 310
MalI GATC 3 cut(s) 5, 177, 249
MboI GATC 3 cut(s) 3, 175, 247
MboII GAAGA 1 cut(s) 295
MflI RGATCY 2 cut(s) 175, 247
MluCI AATT 2 cut(s) 103, 181
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 5 cut(s) 298, 301, 310, 313, 378
Mph1103I ATGCAT 1 cut(s) 217
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 2 cut(s) 375, 377
MspI CCGG 3 cut(s) 64, 420, 436
MwoI GCNNNNNNNGC 2 cut(s) 94, 275
NdeII GATC 3 cut(s) 3, 175, 247
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 3 cut(s) 62, 177, 249
NmuCI GTSAC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 1 cut(s) 358
OliI CACNNNNGTG 2 cut(s) 375, 377
PfeI GAWTC 1 cut(s) 344
PinAI ACCGGT 1 cut(s) 435
PkrI GCNGC 5 cut(s) 60, 90, 93, 96, 391
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PspEI GGTNACC 1 cut(s) 310
PspN4I GGNNCC 3 cut(s) 62, 177, 249
PstI CTGCAG 1 cut(s) 78
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 175, 247
RseI CAYNNNNRTG 2 cut(s) 375, 377
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 5 cut(s) 59, 89, 92, 95, 390
Sau3AI GATC 3 cut(s) 3, 175, 247
SchI GAGTC 1 cut(s) 76
SetI ASST 4 cut(s) 157, 271, 312, 405
SfaNI GCATC 4 cut(s) 199, 202, 224, 322
SfcI CTRYAG 1 cut(s) 74
SmiMI CAYNNNNRTG 2 cut(s) 375, 377
Sse9I AATT 2 cut(s) 103, 181
SsiI CCGC 4 cut(s) 58, 88, 94, 389
SspMI CTAG 1 cut(s) 194
TaqII GACCGA 1 cut(s) 329
TasI AATT 2 cut(s) 103, 181
TauI GCSGC 4 cut(s) 61, 91, 97, 392
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 4 cut(s) 87, 143, 225, 379
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 129
TspRI CASTG 4 cut(s) 87, 143, 225, 379
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.