Rorug03G0285200

ubiquitin protein ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006692
Physical Location & Seq
Forward (+)
28706627 .. 28708416
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0285200.1

Sequence Viewer

Length: 591 bp
ATGCTCACTTCTTCCAAGGCCAAGGCTACAGTGATGAGATCTTTAGAAGATATCATGGCTTTATTAGTCTTCATTTTCTCTCAGGTAAACAGAACTTTACTTCTCTATTTTCTTGATTTTTCTCTTGCCGTGGAAGTGTCTGCCGATCTTGAGGCTGGAGGTGCCAGAATCTCGTCGACTATTTCTGTCTCTCGGATAATGGACAGCATTGCCGGATGCTTTGCTCATTACAGTCAACAACCAGGAAAGGAACATGTTCTCACCCGGCTTGGATCCTTAAAGGCACAGGTGAATGTCATCAGAGATACCCAGCAAGACATCAATTCCACCCAGGAAGATCACCATCGCCGTTTGGCAGAGATCGACCGGGAAGTGCATGGCTTCCACAGAGTGTGTGATACGCTCCTTGAGCGTCAGCAGAGGGTATTTGATTACCAGGAGCAAGTGTGTCAAGGCCAAATTCGAGCTGTGGAGTCTTTGAACAGGACTATCGCCGAATTGCTGATAGTTTCTGAGCTTTGTGCGGTGGGTGATCTGAGCTTTGTTATCACATGTCTTGCTTGGAAAAAATATGTCATTAGCAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.1

Weight (kDa)

6.3

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 176
AciI CCGC 1 cut(s) 524
AclWI GGATC 2 cut(s) 267, 280
AcsI RAATTY 1 cut(s) 459
AdeI CACNNNGTG 1 cut(s) 391
AflIII ACRYGT 2 cut(s) 253, 551
AgsI TTSAA 1 cut(s) 481
AjnI CCWGG 3 cut(s) 241, 330, 435
AluBI AGCT 3 cut(s) 467, 517, 540
AluI AGCT 3 cut(s) 467, 517, 540
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 267, 280
AoxI GGCC 2 cut(s) 18, 454
ApoI RAATTY 1 cut(s) 459
Asp700I GAANNNNTTC 1 cut(s) 255
AsuC2I CCSGG 2 cut(s) 265, 368
AsuHPI GGTGA 4 cut(s) 253, 301, 332, 542
BamHI GGATCC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 61
BccI CCATC 1 cut(s) 351
BceAI ACGGC 2 cut(s) 113, 333
BciT130I CCWGG 3 cut(s) 243, 332, 437
BcnI CCSGG 2 cut(s) 265, 368
BcoDI GTCTC 1 cut(s) 193
BfmI CTRYAG 1 cut(s) 27
BglII AGATCT 1 cut(s) 38
Bme1390I CCNGG 5 cut(s) 243, 265, 332, 368, 437
BmiI GGNNCC 2 cut(s) 163, 274
BmrFI CCNGG 5 cut(s) 243, 265, 332, 368, 437
BmsI GCATC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 170, 428
BpuMI CCSGG 2 cut(s) 265, 368
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 4 cut(s) 15, 21, 129, 330
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bse3DI GCAATG 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 3 cut(s) 243, 332, 437
BseDI CCNNGG 4 cut(s) 15, 21, 129, 330
BseGI GGATG 1 cut(s) 221
BseJI GATNNNNATC 1 cut(s) 342
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 3 cut(s) 95, 504, 527
BseYI CCCAGC 1 cut(s) 309
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 2 cut(s) 20, 456
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 367
BsiSI CCGG 3 cut(s) 213, 265, 367
BsmAI GTCTC 1 cut(s) 193
BsnI GGCC 2 cut(s) 20, 456
Bsp143I GATC 6 cut(s) 38, 145, 272, 337, 360, 532
BspACI CCGC 1 cut(s) 524
BspANI GGCC 2 cut(s) 20, 456
BspCNI CTCAG 3 cut(s) 94, 505, 528
BspLI GGNNCC 2 cut(s) 163, 274
BspPI GGATC 2 cut(s) 267, 280
BspT107I GGYRCC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 207
BssECI CCNNGG 4 cut(s) 15, 21, 129, 330
BssMI GATC 6 cut(s) 38, 145, 272, 337, 360, 532
BssT1I CCWWGG 2 cut(s) 15, 21
Bst2UI CCWGG 3 cut(s) 243, 332, 437
Bst4CI ACNGT 2 cut(s) 31, 233
BstDEI CTNAG 3 cut(s) 81, 513, 536
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 221
BstKTI GATC 6 cut(s) 41, 148, 275, 340, 363, 535
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 6 cut(s) 38, 145, 272, 337, 360, 532
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 161, 409
BstNI CCWGG 3 cut(s) 243, 332, 437
BstNSI RCATGY 2 cut(s) 257, 555
BstSCI CCNGG 5 cut(s) 241, 263, 330, 366, 435
BstSFI CTRYAG 1 cut(s) 27
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 2 cut(s) 38, 272
BstYI RGATCY 2 cut(s) 38, 272
BsuRI GGCC 2 cut(s) 20, 456
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 329
BtsCI GGATG 1 cut(s) 221
BtsIMutI CAGTG 1 cut(s) 36
CseI GACGC 1 cut(s) 401
CviAII CATG 4 cut(s) 55, 254, 377, 552
DdeI CTNAG 3 cut(s) 81, 513, 536
DpnI GATC 6 cut(s) 40, 147, 274, 339, 362, 534
DpnII GATC 6 cut(s) 38, 145, 272, 337, 360, 532
DraIII CACNNNGTG 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 15, 21
Eco32I GATATC 1 cut(s) 52
EcoRII CCWGG 3 cut(s) 241, 330, 435
EcoRV GATATC 1 cut(s) 52
EcoT14I CCWWGG 2 cut(s) 15, 21
ErhI CCWWGG 2 cut(s) 15, 21
FaeI CATG 4 cut(s) 58, 257, 380, 555
FaiI YATR 5 cut(s) 56, 255, 378, 553, 573
FatI CATG 4 cut(s) 54, 253, 376, 551
FblI GTMKAC 1 cut(s) 176
FokI GGATG 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 2 cut(s) 20, 456
HapII CCGG 3 cut(s) 213, 265, 367
HgaI GACGC 1 cut(s) 401
Hin1II CATG 4 cut(s) 58, 257, 380, 555
HincII GTYRAC 2 cut(s) 177, 236
HindII GTYRAC 2 cut(s) 177, 236
HinfI GANTC 2 cut(s) 168, 473
HpaII CCGG 3 cut(s) 213, 265, 367
HphI GGTGA 4 cut(s) 253, 301, 332, 542
Hpy166II GTNNAC 3 cut(s) 88, 177, 236
Hpy188I TCNGA 4 cut(s) 195, 302, 514, 537
Hpy188III TCNNGA 2 cut(s) 113, 149
Hpy8I GTNNAC 3 cut(s) 88, 177, 236
Hpy99I CGWCG 1 cut(s) 178
HpyCH4III ACNGT 2 cut(s) 31, 233
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 409
HpyF3I CTNAG 3 cut(s) 81, 513, 536
Hsp92II CATG 4 cut(s) 58, 257, 380, 555
Kzo9I GATC 6 cut(s) 38, 145, 272, 337, 360, 532
LmnI GCTCC 2 cut(s) 408, 439
LweI GCATC 1 cut(s) 206
MalI GATC 6 cut(s) 40, 147, 274, 339, 362, 534
MboI GATC 6 cut(s) 38, 145, 272, 337, 360, 532
MboII GAAGA 4 cut(s) 3, 59, 61, 347
MflI RGATCY 2 cut(s) 38, 272
MluCI AATT 3 cut(s) 322, 459, 497
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 3 cut(s) 145, 152, 414
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 1 cut(s) 278
MspI CCGG 3 cut(s) 213, 265, 367
MspR9I CCNGG 5 cut(s) 243, 265, 332, 368, 437
MvaI CCWGG 3 cut(s) 243, 332, 437
MwoI GCNNNNNNNGC 2 cut(s) 161, 409
NciI CCSGG 2 cut(s) 265, 368
NdeII GATC 6 cut(s) 38, 145, 272, 337, 360, 532
NlaIII CATG 4 cut(s) 58, 257, 380, 555
NlaIV GGNNCC 2 cut(s) 163, 274
NspI RCATGY 2 cut(s) 257, 555
PciI ACATGT 2 cut(s) 253, 551
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 168
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PscI ACATGT 2 cut(s) 253, 551
Psp6I CCWGG 3 cut(s) 241, 330, 435
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 3 cut(s) 241, 330, 435
PspN4I GGNNCC 2 cut(s) 163, 274
PsuI RGATCY 2 cut(s) 38, 272
SalI GTCGAC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 278
Sau3AI GATC 6 cut(s) 38, 145, 272, 337, 360, 532
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 5 cut(s) 243, 265, 332, 368, 437
SetI ASST 6 cut(s) 87, 163, 291, 469, 519, 542
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 149, 407
SmoI CTYRAG 2 cut(s) 149, 407
Sse9I AATT 3 cut(s) 322, 459, 497
SsiI CCGC 1 cut(s) 524
StyD4I CCNGG 5 cut(s) 241, 263, 330, 366, 435
StyI CCWWGG 2 cut(s) 15, 21
TaaI ACNGT 2 cut(s) 31, 233
TaqI TCGA 3 cut(s) 176, 363, 463
TasI AATT 3 cut(s) 322, 459, 497
TfiI GAWTC 1 cut(s) 168
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TscAI CASTG 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 61
TspRI CASTG 1 cut(s) 36
XapI RAATTY 1 cut(s) 459
XceI RCATGY 2 cut(s) 257, 555
XmiI GTMKAC 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.