Rorug04G0301400

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006214
Physical Location & Seq
Reverse (-)
47625864 .. 47660998
35135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0301400.1

Sequence Viewer

Length: 3876 bp
ATGGATGATCAGGAAAACCATATGGATGGAGATCATGATGCAATTGCAGAGCCAGGCACGGCATTAGCAGTGGCCGCAGATTCGAAGAATAATAACCAAGAGGATACAGGCAAGAGCAAGGAGGATATCGATGCAACCGGTTCAGTACCATATTACAAGCTCTTCTCCTTTGCTGATTCTATGGATTACCTGTTAATGTCTGTTGGTACGATCAGTGCTATTGGAAACGGATTATGTATGCCTCTAATGACTGTGATCATGGGAGAAGTGATTAACTCTTTCGGAGAAACTCCGAATACCAAAAAAGTGGTTGGTATAGTCTCCAAGGTTGCTCTAAAATATGTTTACTTGGCTTTTGGGGCTGCAGCAGCTGCATTTTTACAGATGTCTTGCTGGGTGATCACCGGAGAGAGACAGGCGGCACGAATAAGAAGTTTATACTTGAAGACAATCCTAAGGCAAGATGTTGGTTTTTTCGATAAAGAAGTTACCACCGGGGAAATAGTTGGAAGAATTTCAGGTGATACTGTGCTCATTCAAGAGGCCATGGGCGAGAAAGTAGGAAGTATTATCCAGTTAGTTGCAACATTCATAGGAGGCTTTGTCATAGCATTTGCTAAGGGATGGCGTCTCACCCTTGTCATGCTGACCTCTATTCCCCCTCTTGTTTTCTCCGGTGCTGTATTGAGCTTCACTGTAAAGAGGATGACTACTCGTGGACAAGCTGCTTATTCAGTGGGAGCAACTGTGGTAGAGCAGACAATTGGTTCAATCAGAACTGTTGCATCATTTACAGGTGAGAAGCAAGCTGTAACAAAGTACAACAACTCATTAGACAAAGCTTACAAGTCTGGAGTGCAAGAAGGTTTGGCATCAGGGTTTGGTCTTGGCGCGATTGTGCTTATTGTATTCTGCAGCTATGCTTTGGCTGTATGGTATGGCGGAAAAATGATACTTGAACAAGGATATAAAGCAGGAGATGTCATGAATGTGATATTTGCAGTATTAACTGGCTCCTTCTCTCTCGGACAAGCATCTCCATGCATAAGTGCATTTTCGGCTGGACAAGCTGCAGCTTACAAGATGTTTGAGACAATTAACAGAAGGCCGGAGATAGATGCTTCTGATACTAATGGGAAGCAATTAGAAGACATCCGAGGAGAAATAGAATTTAGGGATGTTTATTTTAGTTATCCTGCAAGACCAGATGAACAAATATTCCAAGGATTCTCACTCTCAATATCTAGTGGTGCAACTGCTGCTCTGGTAGGACAGAGCGGAAGTGGTAAATCGACTATAATCAGTTTGATTGAGAGATTTTATGACCCACAGGCAGGTGAAGTTCTTATCGATGGCATTAATCTCAAAGAGTTCCAGCTGAAATGGATCAGACAGAAAATAGGGCTTGTCAGCCAGGAACCTGTTCTGTTTACTTGCAGCATTAAAGATAATATTACCTATGGGAAGGATGGAGCAACTTTTGAAGAAATAAAGGCTGCCGCTGAGCTTGCCAATGCTGCTAAATTCATAGACAAATTACCTCAGGGACTAGATACAATGGTTGGGGAGCATGGAACTCAGCTATCTGGGGGCCAAAAGCAGAGAGTTGCCATAGCTAGAGCAATCCTTAAGAACCCGAGAATTCTGCTTTTAGATGAAGCTACAAGTGCTCTTGATGCAGAATCTGAGAGGGTTGTGCAAGAGGCATTGGACAGAGTTATGGTTAGCCGGACTACTGTGATTGTAGCCCACCGCTTGAGCACAGTGAGGAATGCTGATATGATTGCAGTTATAAATCGCGGAAAAATTGTTGAACAAGGACCACATTCTGAACTAGTTAAGGTTCCTGATGGAGCATATAGCCAGCTTATAAGGTTGCAACAAATTAGCAGTGTGTCAGAACAGAACTCTCAAAATGTTCAGGAAATTAGGCCATTGTCAAACTCTAGAAGAGATAGAAGTCCAAGACTTTCTCCCTTACAATCCATAAGTCGAGGATCATCTGGAAATACTAGTCCCCACTCATTCTCAATCTCACATGGTGCGCCTACTGTAATAGGTGTGCTTGAAATAGCACCAACTGAACCTCACATTCTAGCTGCATCATCAGAACTGCATCCAGAAGTCTCACTTAGCCGCCTAGCTTACCTGAACAAGCCAGAAATCCCAGTGCTAATTCTAGGTACTATAGCTGCAGCAATTAATGGGGTAATATTGCCTATTTTCGGGGTGTTAATATCCAGTATAATCAAGACCTTCTTTGAGCCACCTCATCAACTTCAAAAGGATTCCAAGTTTTGGTCATTAATCTTTATTCTTCTTGGAGTGGCATCTCTCTTGGCATATCCATTGAAAGCATACTTTTTTGCTGTGGCTGGGGGCAAGTTAATAAGACGAGTTCGATCACTATGCTTTGAGAAGGTGATTCACATGGAAGTAAGTTGGTTCGATGAAGCTGAGCACTCAAGTGGTGCAATTGGTGCAAGGCTTTCTGCAGATGCTGCCTCTCTCCGAGGGCTGGTTGGAGATGCTCTAGGTTTGGCTGTTTTGAATGGATCAACTGCAATTGCTGGATTGGTTATTGCTTTTGTGGCAAATTGGCAACTTGCTCTTATAATCCTTATTCTCCTACCTCTACTAGGAATAAGTGGATATTTCCAGGTCCAGTTCCTAAAAGGATTAAGCGCTAGTGCAAAGAAAATGTATGAAGACGCAAGCCAAGTAGCCACAGATGCTGTGGGGAATATTAGAACAATTGCTTCATTTTGTGCTGAAGAAAAGGTTATCAAGTTGTACCACACAAAATGTGAAGGTCCTATCAAGGCAGGGATAAGACAAGGGATAGTCAGTGGGATAGGCTTTGGACTATCGTTCTTCTTGCTCTTCTCTGTATATGCATGTAGTTTTTATGCTGGTGCTCAACTTGTAGGAGCAGGCAAGACAACATTCCCTGATGTTTTTCGGGTTTTCTTTGCTCTAACTATGACAGCTATAGCATTGTCTCACCAGGGTTCCGTTGCCCCTGATTCAATTAAAGGAAAGAGCGCTGCCGCTTCTATATTTGCACTTCTTGACCAAAAATCAAAAATAGATTCTAGTGATGACTCTGGAACAACCATTGTAAATGTGAAGGGAGAAATCCAATTTCGTCATGTTAGTTTCAAGTATCCTAATAGACCTAATGTGCCAATCTTCCGGGATCTTTGCTTGACCATTCACCATGGCAAGACACTTGCTCTGGTGGGAGAAAGTGGAAGTGGAAAATCGACAGTGATCTCATTGCTTCAGAGATTTTATGACCCTGATTCAGGTCATATTACATTAGATAGTGTTGAAATGAAAACTTTACAGTTGAAGTGGCTGAGACAGCAAATGGGGTTGGTGAGCCAGGAGCCTCTATTGTTTAATGACAATATAAGAGCCAACATTGCATATGGAAAGGAAGGGATTGCAACCGAGGCTGAAATTATAGCTGCAGCAGAATTGGCAAATGCCCACAAGTTCATTAGTAGTTTACAACAGGGCTATGATACAATAGTAGGTGAGCGAGGGGTCCAACTTTCTGGCGGACAGAAGCAAAGGGTAGCTATTGCAAGAGCTATAATGAAGGCACCAAAGATATTACTACTAGATGAAGCCACCAGTGCTCTTGATGCTGAATCCGAAAGAGTGGTTCAAGATGCACTGGATAGAGTTATGGTGGATCGAACTACAGTGGTGGTTGCCCATCGGCTATCCACAATTAAGGGTGCAGATTTAATAGCAGTGGTGAAAAATGGAGTCATTGCAGAGAAAGGAAAGCATGAAACTCTGATCGGTATCAAGGGTGGAACTTATGCTTCTTTGGTAGCACTACATGCAGGTGCCTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1291

Amino Acids

138.6

Weight (kDa)

7.6

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 68 - 340 9.5e-60 ABC transporter transmembrane region
ABC_tran PF00005 408 - 556 1.3e-33 ABC transporter
ABC_membrane PF00664 725 - 997 1.5e-61 ABC transporter transmembrane region
ABC_tran PF00005 1066 - 1215 2.1e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1793, 1871, 2615
AarI CACCTGC 2 cut(s) 1325, 3851
Acc36I ACCTGC 2 cut(s) 1325, 3851
AccB1I GGYRCC 2 cut(s) 3610, 3863
AccB7I CCANNNNNTGG 1 cut(s) 2298
AccBSI CCGCTC 1 cut(s) 1278
AccII CGCG 2 cut(s) 893, 1800
AclWI GGATC 5 cut(s) 1394, 2005, 2563, 3207, 3711
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 4 cut(s) 513, 1169, 1523, 1641
AcuI CTGAAG 2 cut(s) 2793, 3269
AcyI GRCGYC 1 cut(s) 628
AdeI CACNNNGTG 1 cut(s) 2042
AfaI GTAC 5 cut(s) 147, 208, 821, 2185, 2795
AfeI AGCGCT 2 cut(s) 2686, 3046
AfiI CCNNNNNNNGG 6 cut(s) 1334, 2225, 2298, 2518, 2639, 3308
AflII CTTAAG 1 cut(s) 1628
AgeI ACCGGT 1 cut(s) 137
AhlI ACTAGT 2 cut(s) 1834, 2012
AjnI CCWGG 5 cut(s) 52, 1413, 2658, 3006, 3387
AleI CACNNNNGTG 1 cut(s) 2466
AloI GAACNNNNNNTCC 2 cut(s) 1203, 1235
Alw21I GWGCWC 6 cut(s) 534, 1672, 1763, 2463, 2920, 3649
Alw26I GTCTC 7 cut(s) 325, 406, 635, 1085, 2131, 3006, 3358
AlwI GGATC 5 cut(s) 1394, 2005, 2563, 3207, 3711
AlwNI CAGNNNCTG 4 cut(s) 371, 1685, 2501, 2735
Ama87I CYCGRG 1 cut(s) 1636
Aor51HI AGCGCT 2 cut(s) 2686, 3046
AoxI GGCC 5 cut(s) 72, 543, 1106, 1591, 1931
ApoI RAATTY 4 cut(s) 513, 1169, 1523, 1641
AseI ATTAAT 3 cut(s) 1359, 2202, 2306
AsiGI ACCGGT 1 cut(s) 137
Asp700I GAANNNNTTC 2 cut(s) 514, 1422
AspLEI GCGC 4 cut(s) 893, 2047, 2687, 3047
AspS9I GGNCC 5 cut(s) 1591, 1820, 2662, 2813, 3553
AsuC2I CCSGG 2 cut(s) 496, 3197
AsuII TTCGAA 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 1636
AvaII GGWCC 4 cut(s) 1820, 2662, 2813, 3553
AxyI CCTNAGG 2 cut(s) 455, 1542
BaeI ACNNNNGTAYC 4 cut(s) 1545, 1578, 3522, 3555
BanI GGYRCC 2 cut(s) 3610, 3863
BauI CACGAG 1 cut(s) 714
BbsI GAAGAC 3 cut(s) 452, 1155, 2715
Bbv12I GWGCWC 6 cut(s) 534, 1672, 1763, 2463, 2920, 3649
BccI CCATC 6 cut(s) 20, 618, 1346, 1463, 1844, 3735
BceAI ACGGC 1 cut(s) 75
BcgI CGANNNNNNTGC 4 cut(s) 1627, 1661, 2391, 2425
BciT130I CCWGG 5 cut(s) 54, 1415, 2660, 3008, 3389
BciVI GTATCC 2 cut(s) 97, 3177
BclI TGATCA 3 cut(s) 7, 255, 399
BcnI CCSGG 2 cut(s) 496, 3197
BcoDI GTCTC 7 cut(s) 325, 406, 635, 1085, 2131, 3006, 3358
BcuI ACTAGT 2 cut(s) 1834, 2012
BfmI CTRYAG 9 cut(s) 363, 913, 1071, 2187, 2193, 2493, 2991, 3474, 3711
BfoI RGCGCY 2 cut(s) 2688, 3048
BfrI CTTAAG 1 cut(s) 1628
BfuAI ACCTGC 2 cut(s) 1325, 3851
BfuI GTATCC 2 cut(s) 97, 3177
BlpI GCTNAGC 2 cut(s) 1503, 2457
Bme1390I CCNGG 7 cut(s) 54, 496, 1415, 2660, 3008, 3197, 3389
Bme18I GGWCC 4 cut(s) 1820, 2662, 2813, 3553
BmeT110I CYCGRG 1 cut(s) 1636
BmgT120I GGNCC 5 cut(s) 1591, 1820, 2662, 2813, 3553
BmiI GGNNCC 9 cut(s) 1015, 1419, 1592, 1845, 3013, 3393, 3554, 3612, 3865
BmrFI CCNGG 7 cut(s) 54, 496, 1415, 2660, 3008, 3197, 3389
BmrI ACTGGG 1 cut(s) 2162
BmuI ACTGGG 1 cut(s) 2162
BpiI GAAGAC 3 cut(s) 452, 1155, 2715
BpmI CTGGAG 1 cut(s) 873
Bpu10I CCTNAGC 1 cut(s) 618
Bpu1102I GCTNAGC 2 cut(s) 1503, 2457
Bpu14I TTCGAA 1 cut(s) 83
BpuEI CTTGAG 2 cut(s) 1777, 2449
BpuMI CCSGG 2 cut(s) 496, 3197
Bsa29I ATCGAT 2 cut(s) 129, 1350
BsaBI GATNNNNATC 2 cut(s) 30, 3818
BsaHI GRCGYC 1 cut(s) 628
BsaJI CCNNGG 9 cut(s) 324, 495, 546, 1156, 1222, 2512, 3007, 3220, 3456
BsaWI WCCGGW 3 cut(s) 137, 404, 674
BsaXI ACNNNNNCTCC 4 cut(s) 588, 618, 732, 762
Bsc4I CCNNNNNNNGG 6 cut(s) 1334, 2225, 2298, 2518, 2639, 3308
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 7 cut(s) 574, 1015, 2168, 2241, 2665, 3642, 3690
Bse21I CCTNAGG 2 cut(s) 455, 1542
Bse3DI GCAATG 3 cut(s) 3278, 3426, 3783
Bse8I GATNNNNATC 2 cut(s) 30, 3818
BseBI CCWGG 5 cut(s) 54, 1415, 2660, 3008, 3389
BseCI ATCGAT 2 cut(s) 129, 1350
BseDI CCNNGG 9 cut(s) 324, 495, 546, 1156, 1222, 2512, 3007, 3220, 3456
BseGI GGATG 8 cut(s) 10, 31, 629, 711, 1152, 1183, 1474, 2116
BseJI GATNNNNATC 2 cut(s) 30, 3818
BseLI CCNNNNNNNGG 6 cut(s) 1334, 2225, 2298, 2518, 2639, 3308
BseMI GCAATG 3 cut(s) 3278, 3426, 3783
BseMII CTCAG 6 cut(s) 1494, 1556, 1592, 1677, 2448, 3353
BseNI ACTGG 7 cut(s) 574, 1015, 2168, 2241, 2665, 3642, 3690
BseRI GAGGAG 1 cut(s) 1173
BseYI CCCAGC 2 cut(s) 393, 2375
BsgI GTGCAG 1 cut(s) 3771
Bsh1236I CGCG 2 cut(s) 893, 1800
BshFI GGCC 5 cut(s) 74, 545, 1108, 1593, 1933
BshNI GGYRCC 2 cut(s) 3610, 3863
BshTI ACCGGT 1 cut(s) 137
BshVI ATCGAT 2 cut(s) 129, 1350
BsiHKAI GWGCWC 6 cut(s) 534, 1672, 1763, 2463, 2920, 3649
BsiHKCI CYCGRG 1 cut(s) 1636
BsiSI CCGG 7 cut(s) 138, 405, 495, 675, 1109, 1729, 3196
BslFI GGGAC 2 cut(s) 1560, 2001
BslI CCNNNNNNNGG 6 cut(s) 1334, 2225, 2298, 2518, 2639, 3308
BsmAI GTCTC 7 cut(s) 325, 406, 635, 1085, 2131, 3006, 3358
BsmBI CGTCTC 1 cut(s) 635
BsmFI GGGAC 2 cut(s) 1560, 2001
BsmI GAATGC 1 cut(s) 1777
BsnI GGCC 5 cut(s) 74, 545, 1108, 1593, 1933
BsoBI CYCGRG 1 cut(s) 1636
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 6 cut(s) 534, 1672, 1763, 2463, 2920, 3649
Bsp1720I GCTNAGC 2 cut(s) 1503, 2457
Bsp19I CCATGG 2 cut(s) 546, 3220
BspANI GGCC 5 cut(s) 74, 545, 1108, 1593, 1933
BspCNI CTCAG 6 cut(s) 1495, 1555, 1591, 1678, 2449, 3354
BspDI ATCGAT 2 cut(s) 129, 1350
BspFNI CGCG 2 cut(s) 893, 1800
BspHI TCATGA 2 cut(s) 34, 984
BspLI GGNNCC 9 cut(s) 1015, 1419, 1592, 1845, 3013, 3393, 3554, 3612, 3865
BspMAI CTGCAG 6 cut(s) 367, 917, 1075, 2197, 2497, 3478
BspMI ACCTGC 2 cut(s) 1325, 3851
BspPI GGATC 5 cut(s) 1394, 2005, 2563, 3207, 3711
BspQI GCTCTTC 2 cut(s) 167, 2888
BspT104I TTCGAA 1 cut(s) 83
BspT107I GGYRCC 2 cut(s) 3610, 3863
BspTI CTTAAG 1 cut(s) 1628
BsrBI CCGCTC 1 cut(s) 1278
BsrDI GCAATG 3 cut(s) 3278, 3426, 3783
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 7 cut(s) 574, 1015, 2168, 2241, 2665, 3642, 3690
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 9 cut(s) 324, 495, 546, 1156, 1222, 2512, 3007, 3220, 3456
BssNI GRCGYC 1 cut(s) 628
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 4 cut(s) 324, 546, 1222, 3220
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 5 cut(s) 54, 1415, 2660, 3008, 3389
Bst6I CTCTTC 3 cut(s) 167, 1945, 2888
BstACI GRCGYC 1 cut(s) 628
BstAFI CTTAAG 1 cut(s) 1628
BstAPI GCANNNNNTGC 5 cut(s) 371, 1259, 2480, 2501, 3857
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 5 cut(s) 807, 1509, 1865, 2716, 2935
BstDSI CCRYGG 2 cut(s) 546, 3220
BstENI CCTNNNNNAGG 2 cut(s) 2637, 3306
BstF5I GGATG 8 cut(s) 10, 31, 629, 711, 1152, 1183, 1474, 2116
BstFNI CGCG 2 cut(s) 893, 1800
BstH2I RGCGCY 2 cut(s) 2688, 3048
BstHHI GCGC 4 cut(s) 893, 2047, 2687, 3047
BstMAI GTCTC 7 cut(s) 325, 406, 635, 1085, 2131, 3006, 3358
BstNI CCWGG 5 cut(s) 54, 1415, 2660, 3008, 3389
BstNSI RCATGY 2 cut(s) 2901, 3860
BstSCI CCNGG 7 cut(s) 52, 494, 1413, 2658, 3006, 3195, 3387
BstSFI CTRYAG 9 cut(s) 363, 913, 1071, 2187, 2193, 2493, 2991, 3474, 3711
BstUI CGCG 2 cut(s) 893, 1800
BstV2I GAAGAC 3 cut(s) 452, 1155, 2715
BstX2I RGATCY 1 cut(s) 3199
BstXI CCANNNNNNTGG 3 cut(s) 26, 307, 3563
BstYI RGATCY 1 cut(s) 3199
Bsu15I ATCGAT 2 cut(s) 129, 1350
Bsu36I CCTNAGG 2 cut(s) 455, 1542
BsuI GTATCC 2 cut(s) 97, 3177
BsuRI GGCC 5 cut(s) 74, 545, 1108, 1593, 1933
BsuTUI ATCGAT 2 cut(s) 129, 1350
BtgI CCRYGG 2 cut(s) 546, 3220
BtsCI GGATG 8 cut(s) 10, 31, 629, 711, 1152, 1183, 1474, 2116
BtsI GCAGTG 3 cut(s) 75, 1897, 3771
BveI ACCTGC 2 cut(s) 1325, 3851
Cac8I GCNNGC 5 cut(s) 807, 1509, 1865, 2716, 2935
CaiI CAGNNNCTG 4 cut(s) 371, 1685, 2501, 2735
CciI TCATGA 2 cut(s) 34, 984
CfoI GCGC 4 cut(s) 893, 2047, 2687, 3047
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 5 cut(s) 1591, 1820, 2662, 2813, 3553
ClaI ATCGAT 2 cut(s) 129, 1350
CseI GACGC 2 cut(s) 617, 2720
Csp6I GTAC 5 cut(s) 146, 207, 820, 2184, 2794
CspAI ACCGGT 1 cut(s) 137
CviQI GTAC 5 cut(s) 146, 207, 820, 2184, 2794
DraIII CACNNNGTG 1 cut(s) 2042
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 3 cut(s) 167, 1945, 2888
EarI CTCTTC 3 cut(s) 167, 1945, 2888
EciI GGCGGA 2 cut(s) 957, 3582
Eco130I CCWWGG 4 cut(s) 324, 546, 1222, 3220
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 4 cut(s) 1820, 2662, 2813, 3553
Eco47III AGCGCT 2 cut(s) 2686, 3046
Eco57I CTGAAG 2 cut(s) 2793, 3269
Eco81I CCTNAGG 2 cut(s) 455, 1542
Eco88I CYCGRG 1 cut(s) 1636
EcoNI CCTNNNNNAGG 2 cut(s) 2637, 3306
EcoO109I RGGNCCY 1 cut(s) 2813
EcoRI GAATTC 1 cut(s) 1641
EcoRII CCWGG 5 cut(s) 52, 1413, 2658, 3006, 3387
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 4 cut(s) 324, 546, 1222, 3220
EcoT22I ATGCAT 2 cut(s) 1046, 2899
ErhI CCWWGG 4 cut(s) 324, 546, 1222, 3220
Esp3I CGTCTC 1 cut(s) 635
FalI AAGNNNNNCTT 4 cut(s) 2115, 2147, 2243, 2275
FaqI GGGAC 2 cut(s) 1560, 2001
FauNDI CATATG 2 cut(s) 21, 3433
FbaI TGATCA 3 cut(s) 7, 255, 399
FokI GGATG 8 cut(s) 17, 38, 636, 718, 1139, 1190, 1481, 2103
GlaI GCGC 4 cut(s) 892, 2046, 2686, 3046
GsaI CCCAGC 2 cut(s) 397, 2379
GsuI CTGGAG 1 cut(s) 873
HaeII RGCGCY 2 cut(s) 2688, 3048
HaeIII GGCC 5 cut(s) 74, 545, 1108, 1593, 1933
HapII CCGG 7 cut(s) 138, 405, 495, 675, 1109, 1729, 3196
HgaI GACGC 2 cut(s) 617, 2720
HhaI GCGC 4 cut(s) 893, 2047, 2687, 3047
Hin1I GRCGYC 1 cut(s) 628
Hin6I GCGC 4 cut(s) 891, 2045, 2685, 3045
HinP1I GCGC 4 cut(s) 891, 2045, 2685, 3045
HindIII AAGCTT 1 cut(s) 840
HpaII CCGG 7 cut(s) 138, 405, 495, 675, 1109, 1729, 3196
Hpy166II GTNNAC 4 cut(s) 346, 719, 1431, 3515
Hpy8I GTNNAC 4 cut(s) 346, 719, 1431, 3515
Hsp92I GRCGYC 1 cut(s) 628
HspAI GCGC 4 cut(s) 891, 2045, 2685, 3045
Ksp22I TGATCA 3 cut(s) 7, 255, 399
LguI GCTCTTC 2 cut(s) 167, 2888
LmnI GCTCC 7 cut(s) 740, 1019, 1472, 1567, 1853, 2930, 3391
MaeIII GTNAC 2 cut(s) 487, 811
MbiI CCGCTC 1 cut(s) 1278
MfeI CAATTG 5 cut(s) 42, 762, 2475, 2565, 2754
MflI RGATCY 1 cut(s) 3199
MhlI GDGCHC 6 cut(s) 534, 1672, 1763, 2463, 2920, 3649
MlyI GAGTC 2 cut(s) 3098, 3789
MmeI TCCRAC 3 cut(s) 487, 2503, 3580
Mph1103I ATGCAT 2 cut(s) 1046, 2899
MroXI GAANNNNTTC 2 cut(s) 514, 1422
MslI CAYNNNNRTG 5 cut(s) 24, 989, 1039, 2466, 3861
MspA1I CMGCKG 3 cut(s) 371, 1378, 1502
MspCI CTTAAG 1 cut(s) 1628
MspI CCGG 7 cut(s) 138, 405, 495, 675, 1109, 1729, 3196
MspR9I CCNGG 7 cut(s) 54, 496, 1415, 2660, 3008, 3197, 3389
MunI CAATTG 5 cut(s) 42, 762, 2475, 2565, 2754
Mva1269I GAATGC 1 cut(s) 1777
MvaI CCWGG 5 cut(s) 54, 1415, 2660, 3008, 3389
MvnI CGCG 2 cut(s) 893, 1800
NciI CCSGG 2 cut(s) 496, 3197
NcoI CCATGG 2 cut(s) 546, 3220
NdeI CATATG 2 cut(s) 21, 3433
NlaIV GGNNCC 9 cut(s) 1015, 1419, 1592, 1845, 3013, 3393, 3554, 3612, 3865
NsiI ATGCAT 2 cut(s) 1046, 2899
NspI RCATGY 2 cut(s) 2901, 3860
NspV TTCGAA 1 cut(s) 83
OliI CACNNNNGTG 1 cut(s) 2466
PagI TCATGA 2 cut(s) 34, 984
PaqCI CACCTGC 2 cut(s) 1325, 3851
PciSI GCTCTTC 2 cut(s) 167, 2888
PctI GAATGC 1 cut(s) 1777
PdmI GAANNNNTTC 2 cut(s) 514, 1422
PflMI CCANNNNNTGG 1 cut(s) 2298
PfoI TCCNGGA 1 cut(s) 3195
PinAI ACCGGT 1 cut(s) 137
PleI GAGTC 2 cut(s) 3098, 3788
PpsI GAGTC 2 cut(s) 3098, 3788
PpuMI RGGWCCY 1 cut(s) 2813
PshBI ATTAAT 3 cut(s) 1359, 2202, 2306
PsiI TTATAA 3 cut(s) 1793, 1871, 2615
Psp5II RGGWCCY 1 cut(s) 2813
Psp6I CCWGG 5 cut(s) 52, 1413, 2658, 3006, 3387
PspFI CCCAGC 2 cut(s) 393, 2375
PspGI CCWGG 5 cut(s) 52, 1413, 2658, 3006, 3387
PspN4I GGNNCC 9 cut(s) 1015, 1419, 1592, 1845, 3013, 3393, 3554, 3612, 3865
PspPI GGNCC 5 cut(s) 1591, 1820, 2662, 2813, 3553
PspPPI RGGWCCY 1 cut(s) 2813
PstI CTGCAG 6 cut(s) 367, 917, 1075, 2197, 2497, 3478
PstNI CAGNNNCTG 4 cut(s) 371, 1685, 2501, 2735
PsuI RGATCY 1 cut(s) 3199
PvuII CAGCTG 2 cut(s) 371, 1378
RsaI GTAC 5 cut(s) 147, 208, 821, 2185, 2795
RsaNI GTAC 5 cut(s) 146, 207, 820, 2184, 2794
RseI CAYNNNNRTG 5 cut(s) 24, 989, 1039, 2466, 3861
SapI GCTCTTC 2 cut(s) 167, 2888
Sau96I GGNCC 5 cut(s) 1591, 1820, 2662, 2813, 3553
SchI GAGTC 2 cut(s) 3098, 3789
ScrFI CCNGG 7 cut(s) 54, 496, 1415, 2660, 3008, 3197, 3389
SduI GDGCHC 6 cut(s) 534, 1672, 1763, 2463, 2920, 3649
SfcI CTRYAG 9 cut(s) 363, 913, 1071, 2187, 2193, 2493, 2991, 3474, 3711
SfuI TTCGAA 1 cut(s) 83
SinI GGWCC 4 cut(s) 1820, 2662, 2813, 3553
SmiMI CAYNNNNRTG 5 cut(s) 24, 989, 1039, 2466, 3861
SmlI CTYRAG 3 cut(s) 1628, 1756, 2464
SmoI CTYRAG 3 cut(s) 1628, 1756, 2464
SpeI ACTAGT 2 cut(s) 1834, 2012
SspI AATATT 4 cut(s) 1218, 1453, 2214, 2746
StyD4I CCNGG 7 cut(s) 52, 494, 1413, 2658, 3006, 3195, 3387
StyI CCWWGG 4 cut(s) 324, 546, 1222, 3220
TatI WGTACW 1 cut(s) 819
TauI GCSGC 5 cut(s) 77, 422, 1502, 2139, 3053
TspGWI ACGGA 2 cut(s) 243, 3004
Van91I CCANNNNNTGG 1 cut(s) 2298
Vha464I CTTAAG 1 cut(s) 1628
VpaK11BI GGWCC 4 cut(s) 1820, 2662, 2813, 3553
VspI ATTAAT 3 cut(s) 1359, 2202, 2306
XagI CCTNNNNNAGG 2 cut(s) 2637, 3306
XapI RAATTY 4 cut(s) 513, 1169, 1523, 1641
XbaI TCTAGA 1 cut(s) 1946
XceI RCATGY 2 cut(s) 2901, 3860
XcmI CCANNNNNNNNNTGG 1 cut(s) 2734
XmnI GAANNNNTTC 2 cut(s) 514, 1422
Zsp2I ATGCAT 2 cut(s) 1046, 2899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.