Rorug05G0432100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004367
Physical Location & Seq
Forward (+)
60063848 .. 60065117
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0432100.1

Sequence Viewer

Length: 624 bp
ATGCTAATGAATTCGGAGCGGGTTGCGAATCAGTTTTGGGTCTTGAATCTGACCGATCCGGTGACGATCGGGATGGGTCACAGCTACGATCGGAGCTCCATTACGAAGTGGTTCAGAGATGGAAAGCCGATTTGTCTAGAAACAGGGGAGAAAACGAAGAACATGGAGGTGGTGCCTAATTTGGCATTGAAGAGGTTGATTCAGCAGTACTGCAATGAGAATGGGATTCTGTTTGGCTATTCCTGTTGTCGGAATCACGACATTGGGAGGAGTGCAGTTGCAGGTAGCTTGGCAGCTGAGGGAGCTATGAAAATGGTGGTGGAGTTTGTCAGTGGAAGTGTTGGGATTGGATCGAATAAGGTTGCTTTTGAGATTCGGCTTCTTTCTAAAACGAGCATTTTTAATAGGTCTTGTTTGGTGGAAGCTGGAGTGGTGCCGTATTTGTTGAAGCTGTTGGCTTCTGCAGATTCGAAGACTCAAGAGAATGTAGTTGCAGGGTTGTTGAATATGTCGAAGCATTCAAAAGGGAAAGCTGTGATTGTTGAAAATGGGGGTGTGGATTTGGTTGTGGATGTTCTGAATGAGGGTCTCAAAAAGAAGGAAATGGGTTTGATTTTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.56

Weight (kDa)

8.78

Isoelectric Point (pI)

26.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 17 - 74 1.6e-08 U-box domain
Arm PF00514 135 - 171 3.4e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 2 cut(s) 172, 433
AccBSI CCGCTC 1 cut(s) 19
AciI CCGC 1 cut(s) 19
AclWI GGATC 2 cut(s) 50, 358
AcsI RAATTY 1 cut(s) 10
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 6 cut(s) 46, 190, 448, 505, 522, 545
AluBI AGCT 8 cut(s) 84, 96, 288, 296, 305, 425, 451, 533
AluI AGCT 8 cut(s) 84, 96, 288, 296, 305, 425, 451, 533
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 593
AlwI GGATC 2 cut(s) 50, 358
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 470
BanI GGYRCC 2 cut(s) 172, 433
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 305
BccI CCATC 2 cut(s) 67, 113
BceAI ACGGC 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 593
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 462
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
BmcAI AGTACT 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 174, 435
BpiI GAAGAC 1 cut(s) 479
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 593
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 220
BseGI GGATG 2 cut(s) 78, 577
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 283
BseXI GCAGC 1 cut(s) 305
BsgI GTGCAG 1 cut(s) 294
Bsh1285I CGRYCG 2 cut(s) 69, 91
BshNI GGYRCC 2 cut(s) 172, 433
BsiEI CGRYCG 2 cut(s) 69, 91
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 249
BsmAI GTCTC 1 cut(s) 593
BsmI GAATGC 1 cut(s) 517
Bso31I GGTCTC 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 4 cut(s) 55, 66, 88, 350
BspACI CCGC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 174, 435
BspMAI CTGCAG 1 cut(s) 466
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 2 cut(s) 50, 358
BspT104I TTCGAA 1 cut(s) 470
BspT107I GGYRCC 2 cut(s) 172, 433
BspTNI GGTCTC 1 cut(s) 593
BsrBI CCGCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 220
BssMI GATC 4 cut(s) 55, 66, 88, 350
Bst6I CTCTTC 1 cut(s) 185
BstBI TTCGAA 1 cut(s) 470
BstDEI CTNAG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 78, 577
BstKTI GATC 4 cut(s) 58, 69, 91, 353
BstMAI GTCTC 1 cut(s) 593
BstMBI GATC 4 cut(s) 55, 66, 88, 350
BstMCI CGRYCG 2 cut(s) 69, 91
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 305
BstV2I GAAGAC 1 cut(s) 479
BtsCI GGATG 2 cut(s) 78, 577
BtsIMutI CAGTG 1 cut(s) 337
BveI ACCTGC 1 cut(s) 272
Csp6I GTAC 1 cut(s) 208
CviAII CATG 1 cut(s) 163
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 297
DpnI GATC 4 cut(s) 57, 68, 90, 352
DpnII GATC 4 cut(s) 55, 66, 88, 350
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 96
EcoICRI GAGCTC 1 cut(s) 96
EcoRI GAATTC 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 98
FaeI CATG 1 cut(s) 166
FaiI YATR 3 cut(s) 164, 308, 509
FatI CATG 1 cut(s) 162
FauI CCCGC 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 2 cut(s) 85, 584
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 1 cut(s) 137
GluI GCNGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 447
HapII CCGG 1 cut(s) 59
Hin1II CATG 1 cut(s) 166
HinfI GANTC 8 cut(s) 28, 46, 199, 226, 253, 373, 467, 475
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 73
Hpy188I TCNGA 6 cut(s) 16, 51, 93, 116, 252, 579
Hpy188III TCNNGA 5 cut(s) 43, 70, 137, 257, 479
HpyAV CCTTC 1 cut(s) 592
HpyCH4V TGCA 5 cut(s) 213, 275, 281, 464, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 1 cut(s) 297
Hsp92II CATG 1 cut(s) 166
Kzo9I GATC 4 cut(s) 55, 66, 88, 350
LmnI GCTCC 4 cut(s) 16, 93, 101, 302
LpnPI CCDG 6 cut(s) 72, 129, 256, 267, 411, 480
Lsp1109I GCAGC 1 cut(s) 305
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 2 cut(s) 61, 77
MalI GATC 4 cut(s) 57, 68, 90, 352
MbiI CCGCTC 1 cut(s) 19
MboI GATC 4 cut(s) 55, 66, 88, 350
MboII GAAGA 3 cut(s) 169, 202, 484
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 2 cut(s) 10, 178
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 5 cut(s) 160, 186, 261, 292, 577
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 1 cut(s) 302
NdeII GATC 4 cut(s) 55, 66, 88, 350
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 2 cut(s) 174, 435
NmuCI GTSAC 2 cut(s) 61, 77
NspV TTCGAA 1 cut(s) 470
PctI GAATGC 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 7 cut(s) 28, 46, 199, 226, 253, 373, 467
PkrI GCNGC 1 cut(s) 295
Ple19I CGATCG 2 cut(s) 69, 91
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 174, 435
PstI CTGCAG 1 cut(s) 466
PvuI CGATCG 2 cut(s) 69, 91
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 167
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 4 cut(s) 55, 66, 88, 350
ScaI AGTACT 1 cut(s) 209
SchI GAGTC 1 cut(s) 469
SduI GDGCHC 1 cut(s) 98
SfcI CTRYAG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
Sse9I AATT 2 cut(s) 10, 178
SsiI CCGC 1 cut(s) 19
SspMI CTAG 1 cut(s) 137
SstI GAGCTC 1 cut(s) 98
TaqI TCGA 3 cut(s) 353, 470, 512
TaqII GACCGA 1 cut(s) 68
TasI AATT 2 cut(s) 10, 178
TatI WGTACW 1 cut(s) 207
TfiI GAWTC 7 cut(s) 28, 46, 199, 226, 253, 373, 467
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 2 cut(s) 61, 77
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 2 cut(s) 61, 77
TspDTI ATGAA 2 cut(s) 23, 323
TspRI CASTG 1 cut(s) 337
XapI RAATTY 1 cut(s) 10
XbaI TCTAGA 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 1 cut(s) 137
ZrmI AGTACT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.