Rorug05G0432300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004367
Physical Location & Seq
Reverse (-)
60121882 .. 60123446
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0432300.1

Sequence Viewer

Length: 1419 bp
ATGAAGGAAGTTTTCCCACATTTTGAACATTTTTTTTCGGGGTATTACACCTCTGGTGGAATCCAACGACGAGCCAAGCAGTCTTTTCTCTTGGCACGTCCTCTGGTCAAGAAGCCGGTCGCCTTGAATGACCTGAAACGCTTTCTCCGGCGAGTATGGGTCCTGGATAAGGACTTTAAGATTCAAGAACATGCACCAGATCGGTTCGTTCTGGCTTTTGATCTGAGGAAGGATCGTAATAAGGTTCTGAAGGGTGGACCGTGGAGGTTCCAACAAGCCCCGATCGTATTTCAGGAATATGATGGTGTTGCGCCCATGGAAGATATCGCTCTGACCCTACTCTTCTTCTGGGTTCGCATCAACAACATCCCACCGCTGTTTGAGGAGCCTGACATGATTAGAAACATTGCTTCGGTGGCTGGTAAGTTCCTTGAACTGGATGAAAAGCTTTTTGTGAATCAAGGTAAGGTTCGAGTCCATGTGGCTCATGATATCTCCAAACCTTTTTTTCTGAACAAAACCCTAAGACTTGCAGCTGGAGTTGAAGCTGAAATTTCTTATTTTTTTGAGAACTTGGTGGGAAAGTGTGACCATTGTAATCTGATTTATCATGAGGGTGATCATTGCCCTTTGCTTGGGCGTCATACTACATCATATACAGGGGACCAGAGAAAGGATATTGTTGGTCCGTCTTTGGGTTTTGCTGCTAACAAGTTTGTTGATGGAACCTTTCGCTTTCAGGGTGTGCAAACACCGACAAATATTTGCAGGTCTCTATTCCAACCCAAGGAAGCAATCAAACCACGCAAGCCAATTATACTGCGTGCAAGAGACCTCAATCTCTCTGAGACTGATGAGGAAGTCCACCAACCTTTAGCACTGGCGGTGCTTGCAGACCCAGCTAACCATGTATCCAATTCTGAGTCGACAACTCTGACTGAGGGCTTGGAGCTTGAGCAGGTGTCACCATCTGAACCGGCAGAGAGCACTCCTAGCCTGAAAACTTCTGACAACACAGGTGTACCTGCAGAATTGGAGGCAGTGACCAAGGAGAATAGGGGTGACAAGCGTCACCTACCTCCCTCTCCTACAAAATCTCCCCAAAAGCTCAAGTTCCTGCTCCCGGAGCTGTTCAAACCTCTCCTCCCCGAGGTTCATGCCAAGAAACAAAAGATTCCCATGTTCACCAGTAGGTTCTCTGCTAAGTCCATAGGGATGGTTGAAGTGCCTGGCAATATCCTCGTCCCCAAGGAGCTGGTCTCTGCTCACTCTGATGCCAAAAAGAAACGAGGAAGGCCATTAGGGGCAAAGAATAAGAACCCTCCAATGTCGAAGAAGGTGACTGATGCGCAAGAGACTTGCTTTGCTTATCTTTCTAAACCTCCTAGGCCAACTGTTAAGGGCAAGGAGCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

472

Amino Acids

53.24

Weight (kDa)

9.42

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 27 - 101 1.4e-11 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 949
Acc16I TGCGCA 1 cut(s) 1350
Acc36I ACCTGC 3 cut(s) 759, 949, 1033
AccI GTMKAC 1 cut(s) 926
AciI CCGC 2 cut(s) 374, 884
AclWI GGATC 1 cut(s) 240
AcsI RAATTY 2 cut(s) 552, 1413
AcuI CTGAAG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 640
AfaI GTAC 1 cut(s) 1023
AfiI CCNNNNNNNGG 8 cut(s) 169, 436, 635, 673, 695, 787, 1123, 1150
AgsI TTSAA 7 cut(s) 26, 127, 185, 434, 545, 1135, 1223
AjiI CACGTC 1 cut(s) 98
AjnI CCWGG 2 cut(s) 162, 1228
AluBI AGCT 8 cut(s) 448, 536, 548, 902, 952, 1108, 1129, 1255
AluI AGCT 8 cut(s) 448, 536, 548, 902, 952, 1108, 1129, 1255
Alw21I GWGCWC 1 cut(s) 989
Alw26I GTCTC 5 cut(s) 777, 825, 842, 1264, 1349
AlwI GGATC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 1148
AoxI GGCC 2 cut(s) 1295, 1388
ApeKI GCWGC 2 cut(s) 533, 704
ApoI RAATTY 2 cut(s) 552, 1413
AspA2I CCTAGG 1 cut(s) 1385
AspLEI GCGC 2 cut(s) 313, 1351
AspS9I GGNCC 4 cut(s) 160, 257, 664, 686
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 6 cut(s) 629, 957, 1064, 1073, 1177, 1351
AvaI CYCGRG 1 cut(s) 1148
AvaII GGWCC 4 cut(s) 160, 257, 664, 686
AvrII CCTAGG 1 cut(s) 1385
BaeI ACNNNNGTAYC 2 cut(s) 1005, 1038
Bbv12I GWGCWC 1 cut(s) 989
BbvI GCAGC 2 cut(s) 545, 691
BccI CCATC 4 cut(s) 296, 716, 976, 1210
BcgI CGANNNNNNTGC 4 cut(s) 1139, 1173, 1222, 1256
BciT130I CCWGG 2 cut(s) 164, 1230
BciVI GTATCC 1 cut(s) 922
BclI TGATCA 1 cut(s) 619
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 5 cut(s) 777, 825, 842, 1264, 1349
BfaI CTAG 2 cut(s) 993, 1386
BfmI CTRYAG 1 cut(s) 1026
BfuAI ACCTGC 3 cut(s) 759, 949, 1033
BfuI GTATCC 1 cut(s) 922
BisI GCNGC 2 cut(s) 534, 705
BlnI CCTAGG 1 cut(s) 1385
BlsI GCNGC 2 cut(s) 535, 706
Bme1390I CCNGG 3 cut(s) 164, 1124, 1230
Bme18I GGWCC 4 cut(s) 160, 257, 664, 686
BmeT110I CYCGRG 1 cut(s) 1148
BmgBI CACGTC 1 cut(s) 98
BmgT120I GGNCC 4 cut(s) 160, 257, 664, 686
BmiI GGNNCC 5 cut(s) 161, 269, 387, 665, 727
BmrFI CCNGG 3 cut(s) 164, 1124, 1230
BmsI GCATC 3 cut(s) 366, 1264, 1336
BoxI GACNNNNGTC 1 cut(s) 1068
BplI GAGNNNNNCTC 2 cut(s) 1244, 1276
BpmI CTGGAG 1 cut(s) 558
BpuEI CTTGAG 2 cut(s) 974, 1094
BpuMI CCSGG 1 cut(s) 1124
BsaHI GRCGYC 1 cut(s) 640
BsaI GGTCTC 3 cut(s) 777, 825, 1264
BsaJI CCNNGG 7 cut(s) 260, 315, 786, 1047, 1149, 1248, 1385
BsaXI ACNNNNNCTCC 6 cut(s) 129, 159, 1081, 1111, 1128, 1158
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 436, 635, 673, 695, 787, 1123, 1150
Bse118I RCCGGY 2 cut(s) 115, 976
Bse1I ACTGG 3 cut(s) 441, 885, 1188
Bse3DI GCAATG 2 cut(s) 405, 622
BseBI CCWGG 2 cut(s) 164, 1230
BseDI CCNNGG 7 cut(s) 260, 315, 786, 1047, 1149, 1248, 1385
BseGI GGATG 3 cut(s) 366, 445, 1221
BseLI CCNNNNNNNGG 8 cut(s) 169, 436, 635, 673, 695, 787, 1123, 1150
BseMI GCAATG 2 cut(s) 405, 622
BseMII CTCAG 4 cut(s) 215, 837, 912, 930
BseNI ACTGG 3 cut(s) 441, 885, 1188
BseRI GAGGAG 2 cut(s) 398, 1133
BseXI GCAGC 2 cut(s) 545, 691
BseYI CCCAGC 1 cut(s) 898
Bsh1285I CGRYCG 2 cut(s) 120, 285
BshFI GGCC 2 cut(s) 1297, 1390
BsiEI CGRYCG 2 cut(s) 120, 285
BsiHKAI GWGCWC 1 cut(s) 989
BsiHKCI CYCGRG 1 cut(s) 1148
BsiSI CCGG 4 cut(s) 116, 148, 977, 1124
BslFI GGGAC 2 cut(s) 677, 1229
BslI CCNNNNNNNGG 8 cut(s) 169, 436, 635, 673, 695, 787, 1123, 1150
BsmAI GTCTC 5 cut(s) 777, 825, 842, 1264, 1349
BsmFI GGGAC 2 cut(s) 677, 1229
BsnI GGCC 2 cut(s) 1297, 1390
Bso31I GGTCTC 3 cut(s) 777, 825, 1264
BsoBI CYCGRG 1 cut(s) 1148
Bsp1286I GDGCHC 1 cut(s) 989
Bsp143I GATC 5 cut(s) 199, 220, 232, 282, 619
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 2 cut(s) 374, 884
BspANI GGCC 2 cut(s) 1297, 1390
BspCNI CTCAG 4 cut(s) 216, 838, 913, 931
BspHI TCATGA 2 cut(s) 487, 610
BspLI GGNNCC 5 cut(s) 161, 269, 387, 665, 727
BspMAI CTGCAG 1 cut(s) 1030
BspMI ACCTGC 3 cut(s) 759, 949, 1033
BspPI GGATC 1 cut(s) 240
BspTNI GGTCTC 3 cut(s) 777, 825, 1264
BsrDI GCAATG 2 cut(s) 405, 622
BsrFI RCCGGY 2 cut(s) 115, 976
BsrI ACTGG 3 cut(s) 441, 885, 1188
BssAI RCCGGY 2 cut(s) 115, 976
BssECI CCNNGG 7 cut(s) 260, 315, 786, 1047, 1149, 1248, 1385
BssMI GATC 5 cut(s) 199, 220, 232, 282, 619
BssNI GRCGYC 1 cut(s) 640
BssT1I CCWWGG 5 cut(s) 315, 786, 1047, 1248, 1385
Bst2UI CCWGG 2 cut(s) 164, 1230
Bst4CI ACNGT 2 cut(s) 261, 1396
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 640
BstC8I GCNNGC 3 cut(s) 809, 825, 891
BstDEI CTNAG 6 cut(s) 224, 524, 846, 921, 939, 1203
BstDSI CCRYGG 2 cut(s) 260, 315
BstENI CCTNNNNNAGG 2 cut(s) 167, 1148
BstF5I GGATG 3 cut(s) 366, 445, 1221
BstHHI GCGC 2 cut(s) 313, 1351
BstKTI GATC 5 cut(s) 202, 223, 235, 285, 622
BstMAI GTCTC 5 cut(s) 777, 825, 842, 1264, 1349
BstMBI GATC 5 cut(s) 199, 220, 232, 282, 619
BstMCI CGRYCG 2 cut(s) 120, 285
BstMWI GCNNNNNNNGC 5 cut(s) 416, 890, 899, 993, 1126
BstNI CCWGG 2 cut(s) 164, 1230
BstNSI RCATGY 1 cut(s) 194
BstPAI GACNNNNGTC 1 cut(s) 1068
BstSCI CCNGG 3 cut(s) 162, 1122, 1228
BstSFI CTRYAG 1 cut(s) 1026
BstV1I GCAGC 2 cut(s) 545, 691
BstXI CCANNNNNNTGG 2 cut(s) 1216, 1255
BsuI GTATCC 1 cut(s) 922
BsuRI GGCC 2 cut(s) 1297, 1390
BtgI CCRYGG 2 cut(s) 260, 315
BtrI CACGTC 1 cut(s) 98
BtsCI GGATG 3 cut(s) 366, 445, 1221
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 2 cut(s) 878, 1047
BveI ACCTGC 3 cut(s) 759, 949, 1033
Cac8I GCNNGC 3 cut(s) 809, 825, 891
CciI TCATGA 2 cut(s) 487, 610
CfoI GCGC 2 cut(s) 313, 1351
Cfr10I RCCGGY 2 cut(s) 115, 976
Cfr13I GGNCC 4 cut(s) 160, 257, 664, 686
CseI GACGC 2 cut(s) 629, 1058
Csp6I GTAC 1 cut(s) 1022
CviAII CATG 9 cut(s) 191, 316, 394, 479, 488, 611, 908, 1157, 1180
CviQI GTAC 1 cut(s) 1022
DdeI CTNAG 6 cut(s) 224, 524, 846, 921, 939, 1203
DpnI GATC 5 cut(s) 201, 222, 234, 284, 621
DpnII GATC 5 cut(s) 199, 220, 232, 282, 619
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco130I CCWWGG 5 cut(s) 315, 786, 1047, 1248, 1385
Eco31I GGTCTC 3 cut(s) 777, 825, 1264
Eco32I GATATC 2 cut(s) 325, 493
Eco47I GGWCC 4 cut(s) 160, 257, 664, 686
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 1148
EcoNI CCTNNNNNAGG 2 cut(s) 167, 1148
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 162, 1228
EcoRV GATATC 2 cut(s) 325, 493
EcoT14I CCWWGG 5 cut(s) 315, 786, 1047, 1248, 1385
ErhI CCWWGG 5 cut(s) 315, 786, 1047, 1248, 1385
FaeI CATG 9 cut(s) 194, 319, 397, 482, 491, 614, 911, 1160, 1183
FaqI GGGAC 2 cut(s) 677, 1229
FatI CATG 9 cut(s) 190, 315, 393, 478, 487, 610, 907, 1156, 1179
FbaI TGATCA 1 cut(s) 619
FblI GTMKAC 1 cut(s) 926
Fnu4HI GCNGC 2 cut(s) 534, 705
FokI GGATG 3 cut(s) 353, 452, 1228
Fsp4HI GCNGC 2 cut(s) 534, 705
FspBI CTAG 2 cut(s) 993, 1386
FspI TGCGCA 1 cut(s) 1350
GlaI GCGC 2 cut(s) 312, 1350
GluI GCNGC 2 cut(s) 534, 705
GsaI CCCAGC 1 cut(s) 902
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 1297, 1390
HapII CCGG 4 cut(s) 116, 148, 977, 1124
HgaI GACGC 2 cut(s) 629, 1058
HhaI GCGC 2 cut(s) 313, 1351
Hin1I GRCGYC 1 cut(s) 640
Hin1II CATG 9 cut(s) 194, 319, 397, 482, 491, 614, 911, 1160, 1183
Hin6I GCGC 2 cut(s) 311, 1349
HinP1I GCGC 2 cut(s) 311, 1349
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 6 cut(s) 60, 181, 457, 474, 923, 1174
HpaII CCGG 4 cut(s) 116, 148, 977, 1124
HphI GGTGA 6 cut(s) 629, 957, 1064, 1073, 1177, 1351
Hpy166II GTNNAC 5 cut(s) 257, 865, 927, 1022, 1185
Hpy188III TCNNGA 5 cut(s) 109, 185, 293, 488, 611
Hpy8I GTNNAC 5 cut(s) 257, 865, 927, 1022, 1185
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 4 cut(s) 223, 244, 1287, 1330
HpyCH4III ACNGT 2 cut(s) 261, 1396
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 7 cut(s) 194, 533, 748, 768, 827, 893, 1028
HpyF10VI GCNNNNNNNGC 5 cut(s) 416, 890, 899, 993, 1126
HpyF3I CTNAG 6 cut(s) 224, 524, 846, 921, 939, 1203
HpySE526I ACGT 1 cut(s) 97
Hsp92I GRCGYC 1 cut(s) 640
Hsp92II CATG 9 cut(s) 194, 319, 397, 482, 491, 614, 911, 1160, 1183
HspAI GCGC 2 cut(s) 311, 1349
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 5 cut(s) 199, 220, 232, 282, 619
LmnI GCTCC 6 cut(s) 385, 949, 1125, 1126, 1252, 1408
Lsp1109I GCAGC 2 cut(s) 545, 691
LweI GCATC 3 cut(s) 366, 1264, 1336
MaeI CTAG 2 cut(s) 993, 1386
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 6 cut(s) 587, 963, 1042, 1061, 1070, 1339
MalI GATC 5 cut(s) 201, 222, 234, 284, 621
MboI GATC 5 cut(s) 199, 220, 232, 282, 619
MboII GAAGA 4 cut(s) 332, 334, 337, 1345
MhlI GDGCHC 1 cut(s) 989
MluCI AATT 5 cut(s) 552, 813, 916, 1031, 1413
MlyI GAGTC 2 cut(s) 483, 932
MmeI TCCRAC 3 cut(s) 88, 295, 805
MseI TTAA 3 cut(s) 177, 1398, 1417
MslI CAYNNNNRTG 3 cut(s) 615, 1214, 1272
MspA1I CMGCKG 2 cut(s) 376, 536
MspI CCGG 4 cut(s) 116, 148, 977, 1124
MspR9I CCNGG 3 cut(s) 164, 1124, 1230
MvaI CCWGG 2 cut(s) 164, 1230
MwoI GCNNNNNNNGC 5 cut(s) 416, 890, 899, 993, 1126
NciI CCSGG 1 cut(s) 1124
NcoI CCATGG 1 cut(s) 315
NdeII GATC 5 cut(s) 199, 220, 232, 282, 619
NlaIII CATG 9 cut(s) 194, 319, 397, 482, 491, 614, 911, 1160, 1183
NlaIV GGNNCC 5 cut(s) 161, 269, 387, 665, 727
NmuCI GTSAC 6 cut(s) 587, 963, 1042, 1061, 1070, 1339
NsbI TGCGCA 1 cut(s) 1350
NspI RCATGY 1 cut(s) 194
PagI TCATGA 2 cut(s) 487, 610
PaqCI CACCTGC 1 cut(s) 949
PfeI GAWTC 4 cut(s) 60, 181, 457, 1174
PfoI TCCNGGA 2 cut(s) 162, 1122
PkrI GCNGC 2 cut(s) 535, 706
Ple19I CGATCG 1 cut(s) 285
PleI GAGTC 2 cut(s) 482, 931
PpsI GAGTC 2 cut(s) 482, 931
PpuMI RGGWCCY 1 cut(s) 160
PshAI GACNNNNGTC 1 cut(s) 1068
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 2 cut(s) 162, 1228
PspFI CCCAGC 1 cut(s) 898
PspGI CCWGG 2 cut(s) 162, 1228
PspN4I GGNNCC 5 cut(s) 161, 269, 387, 665, 727
PspPI GGNCC 4 cut(s) 160, 257, 664, 686
PspPPI RGGWCCY 1 cut(s) 160
PstI CTGCAG 1 cut(s) 1030
PvuI CGATCG 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 1 cut(s) 1023
RsaNI GTAC 1 cut(s) 1022
RseI CAYNNNNRTG 3 cut(s) 615, 1214, 1272
SalI GTCGAC 1 cut(s) 925
SaqAI TTAA 3 cut(s) 177, 1398, 1417
SatI GCNGC 2 cut(s) 534, 705
Sau3AI GATC 5 cut(s) 199, 220, 232, 282, 619
Sau96I GGNCC 4 cut(s) 160, 257, 664, 686
SchI GAGTC 2 cut(s) 483, 932
ScrFI CCNGG 3 cut(s) 164, 1124, 1230
SduI GDGCHC 1 cut(s) 989
SfaNI GCATC 3 cut(s) 366, 1264, 1336
SfcI CTRYAG 1 cut(s) 1026
SinI GGWCC 4 cut(s) 160, 257, 664, 686
SmiMI CAYNNNNRTG 3 cut(s) 615, 1214, 1272
SmlI CTYRAG 2 cut(s) 953, 1109
SmoI CTYRAG 2 cut(s) 953, 1109
Sse9I AATT 5 cut(s) 552, 813, 916, 1031, 1413
SsiI CCGC 2 cut(s) 374, 884
SspI AATATT 1 cut(s) 763
SspMI CTAG 2 cut(s) 993, 1386
StyD4I CCNGG 3 cut(s) 162, 1122, 1228
StyI CCWWGG 5 cut(s) 315, 786, 1047, 1248, 1385
TaaI ACNGT 2 cut(s) 261, 1396
TaiI ACGT 1 cut(s) 100
TaqI TCGA 3 cut(s) 472, 926, 1331
TasI AATT 5 cut(s) 552, 813, 916, 1031, 1413
TfiI GAWTC 4 cut(s) 60, 181, 457, 1174
Tru1I TTAA 3 cut(s) 177, 1398, 1417
Tru9I TTAA 3 cut(s) 177, 1398, 1417
TscAI CASTG 2 cut(s) 885, 1047
TseFI GTSAC 6 cut(s) 587, 963, 1042, 1061, 1070, 1339
TseI GCWGC 2 cut(s) 533, 704
Tsp45I GTSAC 6 cut(s) 587, 963, 1042, 1061, 1070, 1339
TspDTI ATGAA 3 cut(s) 17, 456, 1145
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 2 cut(s) 885, 1047
VpaK11BI GGWCC 4 cut(s) 160, 257, 664, 686
XagI CCTNNNNNAGG 2 cut(s) 167, 1148
XapI RAATTY 2 cut(s) 552, 1413
XceI RCATGY 1 cut(s) 194
XmaJI CCTAGG 1 cut(s) 1385
XmiI GTMKAC 1 cut(s) 926
XspI CTAG 2 cut(s) 993, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.