Rorug05G0539700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016406
Physical Location & Seq
Forward (+)
72803660 .. 72804712
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0539700.1

Sequence Viewer

Length: 627 bp
ATGGTGTCTCAGGACACAATTCCTTCACTTGGCAAGTGTCAGAGCCTCATTTGGCTTGACTTAAGCCGCAATGATCTAAATGGTTCCATCCCATCGGAGCTCGCCAGTCAAGCTGGCTTTGTTCTTCCTGGAATTGTTTCGGAGAAGCAGTTTGCATATATTAGAAATGAGGGTGGTACAGCTTGCACGGGTGCAGGAGGCCTAGTTGAGTTTGAGGGAGTTCGACTTGAGAGACTCGAAAGTTTACCTATGGTTCATTCTTGCCCATCAACTAGAATTTACACAGGGTTGACAGTTTACACATTCACCAGCAATGGAAGCATGATCTTCCTTGACATTTCTTACAATTCCTTGTCTGGGACTATTCCCAGAATCTTAGGTAAAATGTCCTATTTGCAAGTCTTGAATTTAGGACACAACATGCTAGGTGGAAATATTCCGGACAGCTTTAGAGGATTGAAAGCTATTGGAGTTCTGGATCTCTCTCACAATAACCTTCAGGGAATTTTGCCAGGGTCTTTTGAGACTCTCTCATTTGACTCATTTCTGAGTGACCTCGAACAACAACCTCACCGGTACAATCCCTTCCGGAGGTCAGCTCACCACTTTCCCAGCATCAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.75

Weight (kDa)

6.28

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Island PF20141 39 - 103 1.8e-30 Brassinosteroid receptor island domain
LRR_8 PF13855 131 - 177 8.2e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 439, 588
AciI CCGC 1 cut(s) 67
AclWI GGATC 1 cut(s) 486
AcsI RAATTY 3 cut(s) 276, 406, 504
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 2 cut(s) 178, 578
AfiI CCNNNNNNNGG 3 cut(s) 29, 357, 591
AflII CTTAAG 1 cut(s) 61
AgeI ACCGGT 1 cut(s) 573
AgsI TTSAA 2 cut(s) 406, 460
AjnI CCWGG 2 cut(s) 127, 511
AluBI AGCT 6 cut(s) 100, 113, 182, 447, 464, 599
AluI AGCT 6 cut(s) 100, 113, 182, 447, 464, 599
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 3 cut(s) 12, 226, 518
AlwI GGATC 1 cut(s) 486
Aor13HI TCCGGA 2 cut(s) 439, 588
AoxI GGCC 1 cut(s) 199
ApoI RAATTY 3 cut(s) 276, 406, 504
AsiGI ACCGGT 1 cut(s) 573
Asp700I GAANNNNTTC 1 cut(s) 136
AsuHPI GGTGA 3 cut(s) 298, 563, 593
BanII GRGCYC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 569, 601
Bbv12I GWGCWC 1 cut(s) 102
BccI CCATC 3 cut(s) 95, 100, 274
BciT130I CCWGG 2 cut(s) 129, 513
BcoDI GTCTC 3 cut(s) 12, 226, 518
BfaI CTAG 3 cut(s) 203, 273, 425
BfrI CTTAAG 1 cut(s) 61
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 129, 513
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 2 cut(s) 129, 513
BplI GAGNNNNNCTC 4 cut(s) 515, 547, 583, 615
BpuEI CTTGAG 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 512
BsaWI WCCGGW 3 cut(s) 439, 573, 588
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 357, 591
Bse118I RCCGGY 1 cut(s) 573
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 2 cut(s) 76, 319
BseAI TCCGGA 2 cut(s) 439, 588
BseBI CCWGG 2 cut(s) 129, 513
BseDI CCNNGG 1 cut(s) 512
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 3 cut(s) 29, 357, 591
BseMI GCAATG 2 cut(s) 76, 319
BseMII CTCAG 2 cut(s) 23, 539
BseNI ACTGG 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 573
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 3 cut(s) 440, 574, 589
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 3 cut(s) 29, 357, 591
BsmAI GTCTC 3 cut(s) 12, 226, 518
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 102
Bsp13I TCCGGA 2 cut(s) 439, 588
Bsp143I GATC 3 cut(s) 73, 324, 478
BspACI CCGC 1 cut(s) 67
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 2 cut(s) 22, 540
BspEI TCCGGA 2 cut(s) 439, 588
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 486
BspTI CTTAAG 1 cut(s) 61
BsrDI GCAATG 2 cut(s) 76, 319
BsrFI RCCGGY 1 cut(s) 573
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 512
BssMI GATC 3 cut(s) 73, 324, 478
Bst2UI CCWGG 2 cut(s) 129, 513
Bst4CI ACNGT 1 cut(s) 295
BstAFI CTTAAG 1 cut(s) 61
BstC8I GCNNGC 3 cut(s) 102, 115, 184
BstDEI CTNAG 3 cut(s) 9, 376, 548
BstENI CCTNNNNNAGG 1 cut(s) 589
BstF5I GGATG 1 cut(s) 87
BstKTI GATC 3 cut(s) 76, 327, 481
BstMAI GTCTC 3 cut(s) 12, 226, 518
BstMBI GATC 3 cut(s) 73, 324, 478
BstMWI GCNNNNNNNGC 2 cut(s) 110, 318
BstNI CCWGG 2 cut(s) 129, 513
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 127, 511
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 87
Cac8I GCNNGC 3 cut(s) 102, 115, 184
Cfr10I RCCGGY 1 cut(s) 573
Csp6I GTAC 2 cut(s) 177, 577
CspAI ACCGGT 1 cut(s) 573
CviAII CATG 2 cut(s) 322, 421
CviQI GTAC 2 cut(s) 177, 577
DdeI CTNAG 3 cut(s) 9, 376, 548
DpnI GATC 3 cut(s) 75, 326, 480
DpnII GATC 3 cut(s) 73, 324, 478
Ecl136II GAGCTC 1 cut(s) 100
Eco147I AGGCCT 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 482
EcoICRI GAGCTC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 127, 511
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 2 cut(s) 325, 424
FaiI YATR 6 cut(s) 157, 159, 251, 323, 422, 625
FaqI GGGAC 1 cut(s) 373
FatI CATG 2 cut(s) 321, 420
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 67
FspBI CTAG 3 cut(s) 203, 273, 425
GluI GCNGC 1 cut(s) 67
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 1 cut(s) 201
HapII CCGG 3 cut(s) 440, 574, 589
Hin1II CATG 2 cut(s) 325, 424
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 4 cut(s) 234, 372, 526, 539
HpaII CCGG 3 cut(s) 440, 574, 589
HphI GGTGA 3 cut(s) 298, 563, 593
Hpy166II GTNNAC 3 cut(s) 245, 291, 298
Hpy188I TCNGA 4 cut(s) 42, 97, 142, 549
Hpy188III TCNNGA 6 cut(s) 11, 403, 440, 476, 589, 619
Hpy8I GTNNAC 3 cut(s) 245, 291, 298
HpyAV CCTTC 3 cut(s) 33, 506, 595
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 4 cut(s) 155, 186, 194, 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 318
HpyF3I CTNAG 3 cut(s) 9, 376, 548
Hsp92II CATG 2 cut(s) 325, 424
Kpn2I TCCGGA 2 cut(s) 439, 588
Kzo9I GATC 3 cut(s) 73, 324, 478
LmnI GCTCC 1 cut(s) 97
MaeI CTAG 3 cut(s) 203, 273, 425
MaeIII GTNAC 1 cut(s) 551
MalI GATC 3 cut(s) 75, 326, 480
MboI GATC 3 cut(s) 73, 324, 478
MboII GAAGA 2 cut(s) 116, 319
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 6 cut(s) 18, 132, 276, 346, 406, 504
MlyI GAGTC 3 cut(s) 228, 520, 533
MnlI CCTC 8 cut(s) 56, 163, 191, 208, 446, 566, 579, 585
MroI TCCGGA 2 cut(s) 439, 588
MroXI GAANNNNTTC 1 cut(s) 136
MseI TTAA 1 cut(s) 62
MspCI CTTAAG 1 cut(s) 61
MspI CCGG 3 cut(s) 440, 574, 589
MspR9I CCNGG 2 cut(s) 129, 513
MvaI CCWGG 2 cut(s) 129, 513
MwoI GCNNNNNNNGC 2 cut(s) 110, 318
NdeII GATC 3 cut(s) 73, 324, 478
NlaIII CATG 2 cut(s) 325, 424
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 551
NspI RCATGY 1 cut(s) 424
PceI AGGCCT 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 372
PfoI TCCNGGA 1 cut(s) 127
PinAI ACCGGT 1 cut(s) 573
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 3 cut(s) 228, 520, 533
PpsI GAGTC 3 cut(s) 228, 520, 533
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 127, 511
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 2 cut(s) 127, 511
PspN4I GGNNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 2 cut(s) 178, 578
RsaNI GTAC 2 cut(s) 177, 577
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 3 cut(s) 73, 324, 478
SchI GAGTC 3 cut(s) 228, 520, 533
ScrFI CCNGG 2 cut(s) 129, 513
SduI GDGCHC 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 61, 227
SmoI CTYRAG 2 cut(s) 61, 227
Sse9I AATT 6 cut(s) 18, 132, 276, 346, 406, 504
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 1 cut(s) 67
SspI AATATT 1 cut(s) 436
SspMI CTAG 3 cut(s) 203, 273, 425
SstI GAGCTC 1 cut(s) 102
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 127, 511
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 223, 237, 558
TasI AATT 6 cut(s) 18, 132, 276, 346, 406, 504
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 372
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseFI GTSAC 1 cut(s) 551
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 1 cut(s) 245
Vha464I CTTAAG 1 cut(s) 61
XagI CCTNNNNNAGG 1 cut(s) 589
XapI RAATTY 3 cut(s) 276, 406, 504
XceI RCATGY 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 136
XspI CTAG 3 cut(s) 203, 273, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.