Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0002794

Physical Location & Seq

Genomic Coordinates Forward (+)

12670186 .. 12671640

Gene Structure 1455 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0094700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGTCATGCTCTCTCTATATTTGTCTTACAGTAACTTGGGACACTACCTCCGCTGGTTTACCAGGTCTGTTTCCTTCAAGCAATTACATGAACACAAAATATCTGCAGGCAAGAGAAAACAAATCGAGAACTGCAACGACGACCAAATTACTTGTCATGGGTCAGTTGGGTGCTGGCAGACACCGCCACAAGACATCATGGAGGAGATTCTACAGAAAATGGGTTTAATTGCTCGTACACGCTTAGCCAAGGTCTGCATGTCTTGGAGTTCACTTGTTATGAGAGCTGATATCCGCAGTGCTCCGCAACTGCCATGGTTAGTACACCCTCAAACCCCAAACACCAACTACTTAACCTTTTCCAGCCTCTCTGAGGGTGAAGTTGTGAACTTGGAGCTCCCAAAGCGATTCCGAGGACAAGGCAAGTTCAAGTTTCGAGCGTCTTCTAAAGGTTGGTTGATCATTTTACAAGAAAAAGGATTCGGCTTTAATATGTTCCTATACAATCCGATTTCTAGAGCCCAACACCAACTTCCTTCCTTAAACACTATTCCATCTTTCCTATCTTTCCAACTAGCAAGAATGCTAGGGAAGGTTATCAACAATCATGCCGAGAATTTTGTATCTAAAGTTGTGTTGTCTAGTCCAAATGTCTCAGAATGCATTGTGGCTGCCGTTTTCGAACCTCATCATGAATTGGGATTATGCAGACCTGGAGACAAAAGCTGGACTGTCTTTGAGATACTGAATCATGAGGACGACTTCCTCATGGATTTACTTTTTTCGCAGGGCATGCTATACGGCTTAGTTGATAATAGCAAGTCCACCAAGAATGGCATCGGCGCAGCTCGCATCTTAAACTTTGGAGATGATCAGGCAATGACAGTGGAATTGAAGTTGGTCTACGGCCATAAAGAAGTTGGTAATACTACAGAATATCATGATGGCTTTCTAATCTTTTTAAATAGAGACTACAACTCATATTTGTTAGAATCAACAACAAATAGCGACAAGGAAATCTTGTTGATCCATCAAATGCTCGATTGTTTAATCCCCAACTGTGCTGATGATGATGATGATGATGATGATGATGATGATGAGGAAATCGAATATCAGAGGACAAGCGGGTTTGCAGTATACAAGATTGACCTTGAGTATGGCAACTTTCATGAGGTACAAAATTTGGGGGACCAAATAATATTTTTGGCAGAACATAGCTCTTCCTTGTCCTTTCCTGCAAGTGATTTCAAACTACAAGGAAATTGCATTTATTTTGCAACAAGCGGATACGCAACTATCAACCTACCTCGTAAGACCCTTCTTGGATCAAATATATCTCGTGAAATCGGAATCTTTAGTTTAGATGACGGAAAAATTAAGCGACCGTTTCCAAGTCTTGATGTTGCTGGCATTTCATCACCTAGTCAACTGAGTTGGTTTAGTCCAAGTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005986 GO:0005996 GO:0006000 GO:0006002 GO:0006006 GO:0006073 GO:0006094 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009251 GO:0009311 GO:0009312 GO:0009719 GO:0009725 GO:0009737 GO:0009743 GO:0009746 GO:0009750 GO:0009987 GO:0010033 GO:0015979 GO:0016051 GO:0016052 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0019318 GO:0019319 GO:0019637 GO:0030388 GO:0033993 GO:0034284 GO:0034637 GO:0042132 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0046364 GO:0050308 GO:0050896 GO:0071704 GO:0097305 GO:1901135 GO:1901575 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04152 ko04910 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map04152 map04910

Pfam Domains

Protein Families

FBPase

Protein Analysis

484

Amino Acids

54.73

Weight (kDa)

5.46

Isoelectric Point (pI)

41.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_KIB1-4	PF03478	120 - 452	2e-39	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43670
fragaria_vesca	FvH4_2g13890 FvH4_2g13890 FvH4_2g13890 FvH4_2g13890 FvH4_2g13890 FvH4_2g13890
malus_domestica	MD05G1023900.v1.1 MD10G1024400.v1.1 MD15G1416100.v1.1
prunus_persica	Prupe.8G028300_v2.0.a1 Prupe.8G028300_v2.0.a1
pyrus_communis	pycom05g01530 pycom10g01620
rosa_chinensis	RchiOBHm_Chr6g0275641 RchiOBHm_Chr7g0225171 RchiOBHm_Chr7g0225291 RchiOBHm_Chr7g0225311
rosa_laevigata	RLG00000001840 RLG00000013451
rosa_multiflora	Rmu_sc0002011.1_g000002 Rmu_sc0005043.1_g000001 Rmu_sc0010532.1_g000007
rosa_roxburghii	Rroxscaffold_3G00234160 Rroxscaffold_7G00193110
rosa_rugosa	Rorug06G0094600 Rorug06G0094700 Rorug07G0227700 Rorug07G0227700
rosa_samantha	Rh6AG207100 Rh6BG209800 Rh6CG213000 Rh6DG202900 Rh7AG374800 Rh7BG362700 Rh7CG394500
rosa_wichuraiana	Rw0G021340 Rw6G017950 Rw7G031530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	903, 1137
AciI	CCGC	6 cut(s)	52, 185, 295, 305, 1125, 1284
AclWI	GGATC	2 cut(s)	1021, 1333
AcoI	YGGCCR	1 cut(s)	907
AcsI	RAATTY	2 cut(s)	616, 1180
AfaI	GTAC	3 cut(s)	238, 324, 1176
AfiI	CCNNNNNNNGG	1 cut(s)	373
AgsI	TTSAA	4 cut(s)	79, 430, 895, 1249
AjnI	CCWGG	2 cut(s)	62, 712
AjuI	GAANNNNNNNTTGG	2 cut(s)	516, 548
AluBI	AGCT	5 cut(s)	287, 397, 726, 848, 1218
AluI	AGCT	5 cut(s)	287, 397, 726, 848, 1218
Alw21I	GWGCWC	2 cut(s)	304, 399
Alw26I	GTCTC	3 cut(s)	658, 711, 963
AlwI	GGATC	2 cut(s)	1021, 1333
AoxI	GGCC	1 cut(s)	907
ApeKI	GCWGC	2 cut(s)	671, 845
ApoI	RAATTY	2 cut(s)	616, 1180
ArsI	GACNNNNNNTTYG	2 cut(s)	139, 171
AspLEI	GCGC	1 cut(s)	845
AspS9I	GGNCC	1 cut(s)	1189
AsuHPI	GGTGA	2 cut(s)	389, 1410
AsuII	TTCGAA	1 cut(s)	681
AvaII	GGWCC	1 cut(s)	1189
BanII	GRGCYC	2 cut(s)	399, 523
BarI	GAAGNNNNNNTAC	2 cut(s)	887, 919
BauI	CACGAG	1 cut(s)	1338
BbsI	GAAGAC	1 cut(s)	435
Bbv12I	GWGCWC	2 cut(s)	304, 399
BbvI	GCAGC	2 cut(s)	658, 857
BccI	CCATC	3 cut(s)	562, 938, 1038
BceAI	ACGGC	3 cut(s)	659, 817, 922
BciT130I	CCWGG	2 cut(s)	64, 714
BciVI	GTATCC	1 cut(s)	1280
BclI	TGATCA	2 cut(s)	459, 871
BcoDI	GTCTC	3 cut(s)	658, 711, 963
BfaI	CTAG	5 cut(s)	516, 575, 587, 642, 1422
BfmI	CTRYAG	3 cut(s)	105, 212, 930
BfuI	GTATCC	1 cut(s)	1280
BisI	GCNGC	2 cut(s)	672, 846
BlpI	GCTNAGC	1 cut(s)	244
BlsI	GCNGC	2 cut(s)	673, 847
Bme1390I	CCNGG	2 cut(s)	64, 714
Bme18I	GGWCC	1 cut(s)	1189
BmgT120I	GGNCC	1 cut(s)	1189
BmiI	GGNNCC	1 cut(s)	1190
BmrFI	CCNGG	2 cut(s)	64, 714
BmsI	GCATC	2 cut(s)	846, 861
BpiI	GAAGAC	1 cut(s)	435
BpmI	CTGGAG	1 cut(s)	735
Bpu1102I	GCTNAGC	1 cut(s)	244
Bpu14I	TTCGAA	1 cut(s)	681
BpuEI	CTTGAG	1 cut(s)	1172
BsaJI	CCNNGG	3 cut(s)	249, 314, 412
BsaXI	ACNNNNNCTCC	2 cut(s)	33, 63
Bsc4I	CCNNNNNNNGG	1 cut(s)	373
Bse3DI	GCAATG	1 cut(s)	885
BseBI	CCWGG	2 cut(s)	64, 714
BseDI	CCNNGG	3 cut(s)	249, 314, 412
BseLI	CCNNNNNNNGG	1 cut(s)	373
BseMI	GCAATG	1 cut(s)	885
BseMII	CTCAG	3 cut(s)	363, 669, 1421
BseRI	GAGGAG	1 cut(s)	218
BseXI	GCAGC	2 cut(s)	658, 857
Bsh1285I	CGRYCG	1 cut(s)	1385
BshFI	GGCC	1 cut(s)	909
BsiEI	CGRYCG	1 cut(s)	1385
BsiHKAI	GWGCWC	2 cut(s)	304, 399
BslFI	GGGAC	2 cut(s)	54, 1202
BslI	CCNNNNNNNGG	1 cut(s)	373
BsmAI	GTCTC	3 cut(s)	658, 711, 963
BsmFI	GGGAC	2 cut(s)	54, 1202
BsmI	GAATGC	2 cut(s)	588, 665
BsnI	GGCC	1 cut(s)	909
Bsp119I	TTCGAA	1 cut(s)	681
Bsp1286I	GDGCHC	3 cut(s)	304, 399, 523
Bsp143I	GATC	4 cut(s)	459, 871, 1026, 1325
Bsp1720I	GCTNAGC	1 cut(s)	244
Bsp19I	CCATGG	1 cut(s)	314
BspACI	CCGC	6 cut(s)	52, 185, 295, 305, 1125, 1284
BspANI	GGCC	1 cut(s)	909
BspCNI	CTCAG	3 cut(s)	364, 668, 1422
BspHI	TCATGA	4 cut(s)	691, 751, 940, 1168
BspLI	GGNNCC	1 cut(s)	1190
BspMAI	CTGCAG	1 cut(s)	109
BspPI	GGATC	2 cut(s)	1021, 1333
BspQI	GCTCTTC	1 cut(s)	1225
BspT104I	TTCGAA	1 cut(s)	681
BsrDI	GCAATG	1 cut(s)	885
BssECI	CCNNGG	3 cut(s)	249, 314, 412
BssMI	GATC	4 cut(s)	459, 871, 1026, 1325
BssNAI	GTATAC	1 cut(s)	1138
BssSI	CACGAG	1 cut(s)	1338
BssT1I	CCWWGG	2 cut(s)	249, 314
Bst1107I	GTATAC	1 cut(s)	1138
Bst2BI	CACGAG	1 cut(s)	1338
Bst2UI	CCWGG	2 cut(s)	64, 714
Bst4CI	ACNGT	5 cut(s)	32, 733, 886, 1061, 1386
Bst6I	CTCTTC	1 cut(s)	1225
BstAPI	GCANNNNNTGC	1 cut(s)	793
BstBI	TTCGAA	1 cut(s)	681
BstC8I	GCNNGC	5 cut(s)	109, 176, 794, 850, 1408
BstDEI	CTNAG	5 cut(s)	244, 372, 655, 805, 1430
BstDSI	CCRYGG	1 cut(s)	314
BstENI	CCTNNNNNAGG	1 cut(s)	371
BstHHI	GCGC	1 cut(s)	845
BstKTI	GATC	4 cut(s)	462, 874, 1029, 1328
BstMAI	GTCTC	3 cut(s)	658, 711, 963
BstMBI	GATC	4 cut(s)	459, 871, 1026, 1325
BstMCI	CGRYCG	1 cut(s)	1385
BstMWI	GCNNNNNNNGC	4 cut(s)	184, 403, 793, 849
BstNI	CCWGG	2 cut(s)	64, 714
BstNSI	RCATGY	2 cut(s)	262, 796
BstSCI	CCNGG	2 cut(s)	62, 712
BstSFI	CTRYAG	3 cut(s)	105, 212, 930
BstV1I	GCAGC	2 cut(s)	658, 857
BstV2I	GAAGAC	1 cut(s)	435
BstZ17I	GTATAC	1 cut(s)	1138
BsuI	GTATCC	1 cut(s)	1280
BsuRI	GGCC	1 cut(s)	909
BtgI	CCRYGG	1 cut(s)	314
BtsI	GCAGTG	1 cut(s)	304
BtsIMutI	CAGTG	2 cut(s)	304, 891
Cac8I	GCNNGC	5 cut(s)	109, 176, 794, 850, 1408
CciI	TCATGA	4 cut(s)	691, 751, 940, 1168
CfoI	GCGC	1 cut(s)	845
Cfr13I	GGNCC	1 cut(s)	1189
CseI	GACGC	1 cut(s)	429
CsiI	ACCWGGT	1 cut(s)	62
Csp6I	GTAC	3 cut(s)	237, 323, 1175
CspCI	CAANNNNNGTGG	2 cut(s)	867, 902
CviQI	GTAC	3 cut(s)	237, 323, 1175
DdeI	CTNAG	5 cut(s)	244, 372, 655, 805, 1430
DpnI	GATC	4 cut(s)	461, 873, 1028, 1327
DpnII	GATC	4 cut(s)	459, 871, 1026, 1325
DraI	TTTAAA	1 cut(s)	963
EaeI	YGGCCR	1 cut(s)	907
Eam1104I	CTCTTC	1 cut(s)	1225
EarI	CTCTTC	1 cut(s)	1225
Ecl136II	GAGCTC	1 cut(s)	397
Eco130I	CCWWGG	2 cut(s)	249, 314
Eco24I	GRGCYC	2 cut(s)	399, 523
Eco32I	GATATC	1 cut(s)	292
Eco47I	GGWCC	1 cut(s)	1189
Eco53kI	GAGCTC	1 cut(s)	397
EcoICRI	GAGCTC	1 cut(s)	397
EcoNI	CCTNNNNNAGG	1 cut(s)	371
EcoRII	CCWGG	2 cut(s)	62, 712
EcoRV	GATATC	1 cut(s)	292
EcoT14I	CCWWGG	2 cut(s)	249, 314
EcoT22I	ATGCAT	1 cut(s)	665
EcoT38I	GRGCYC	2 cut(s)	399, 523
ErhI	CCWWGG	2 cut(s)	249, 314
FalI	AAGNNNNNCTT	2 cut(s)	1004, 1036
FaqI	GGGAC	2 cut(s)	54, 1202
FauI	CCCGC	1 cut(s)	1118
FbaI	TGATCA	2 cut(s)	459, 871
FblI	GTMKAC	2 cut(s)	903, 1137
Fnu4HI	GCNGC	2 cut(s)	672, 846
FriOI	GRGCYC	2 cut(s)	399, 523
Fsp4HI	GCNGC	2 cut(s)	672, 846
FspBI	CTAG	5 cut(s)	516, 575, 587, 642, 1422
GlaI	GCGC	1 cut(s)	844
GluI	GCNGC	2 cut(s)	672, 846
GsuI	CTGGAG	1 cut(s)	735
HaeIII	GGCC	1 cut(s)	909
HgaI	GACGC	1 cut(s)	429
HhaI	GCGC	1 cut(s)	845
Hin6I	GCGC	1 cut(s)	843
HinP1I	GCGC	1 cut(s)	843
HincII	GTYRAC	1 cut(s)	1427
HindII	GTYRAC	1 cut(s)	1427
HinfI	GANTC	6 cut(s)	208, 408, 480, 748, 992, 1350
HphI	GGTGA	2 cut(s)	389, 1410
Hpy166II	GTNNAC	9 cut(s)	60, 239, 272, 325, 388, 825, 904, 1138, 1427
Hpy188I	TCNGA	6 cut(s)	373, 413, 510, 658, 1116, 1349
Hpy188III	TCNNGA	8 cut(s)	127, 516, 692, 752, 941, 1169, 1340, 1397
Hpy8I	GTNNAC	9 cut(s)	60, 239, 272, 325, 388, 825, 904, 1138, 1427
Hpy99I	CGWCG	1 cut(s)	143
HpyAV	CCTTC	4 cut(s)	85, 546, 586, 1328
HpyCH4III	ACNGT	5 cut(s)	32, 733, 886, 1061, 1386
HpyCH4V	TGCA	9 cut(s)	107, 135, 258, 663, 708, 1133, 1238, 1266, 1277
HpyF10VI	GCNNNNNNNGC	4 cut(s)	184, 403, 793, 849
HpyF3I	CTNAG	5 cut(s)	244, 372, 655, 805, 1430
HspAI	GCGC	1 cut(s)	843
Ksp22I	TGATCA	2 cut(s)	459, 871
Kzo9I	GATC	4 cut(s)	459, 871, 1026, 1325
LguI	GCTCTTC	1 cut(s)	1225
LmnI	GCTCC	3 cut(s)	307, 394, 402
Lsp1109I	GCAGC	2 cut(s)	658, 857
LweI	GCATC	2 cut(s)	846, 861
MabI	ACCWGGT	1 cut(s)	62
MaeI	CTAG	5 cut(s)	516, 575, 587, 642, 1422
MaeIII	GTNAC	1 cut(s)	32
MalI	GATC	4 cut(s)	461, 873, 1028, 1327
MboI	GATC	4 cut(s)	459, 871, 1026, 1325
MboII	GAAGA	2 cut(s)	435, 1212
MhlI	GDGCHC	3 cut(s)	304, 399, 523
MluCI	AATT	9 cut(s)	83, 147, 228, 616, 695, 890, 1180, 1261, 1374
MmeI	TCCRAC	1 cut(s)	595
Mph1103I	ATGCAT	1 cut(s)	665
MseI	TTAA	8 cut(s)	227, 353, 489, 542, 857, 962, 1049, 1377
MspA1I	CMGCKG	1 cut(s)	54
MspR9I	CCNGG	2 cut(s)	64, 714
Mva1269I	GAATGC	2 cut(s)	588, 665
MvaI	CCWGG	2 cut(s)	64, 714
MwoI	GCNNNNNNNGC	4 cut(s)	184, 403, 793, 849
NcoI	CCATGG	1 cut(s)	314
NdeII	GATC	4 cut(s)	459, 871, 1026, 1325
NlaIV	GGNNCC	1 cut(s)	1190
NmeAIII	GCCGAG	1 cut(s)	637
NsiI	ATGCAT	1 cut(s)	665
NspI	RCATGY	2 cut(s)	262, 796
NspV	TTCGAA	1 cut(s)	681
PaeI	GCATGC	1 cut(s)	796
PagI	TCATGA	4 cut(s)	691, 751, 940, 1168
PciSI	GCTCTTC	1 cut(s)	1225
PctI	GAATGC	2 cut(s)	588, 665
PfeI	GAWTC	6 cut(s)	208, 408, 480, 748, 992, 1350
PkrI	GCNGC	2 cut(s)	673, 847
Psp124BI	GAGCTC	1 cut(s)	399
Psp6I	CCWGG	2 cut(s)	62, 712
PspGI	CCWGG	2 cut(s)	62, 712
PspN4I	GGNNCC	1 cut(s)	1190
PspPI	GGNCC	1 cut(s)	1189
PstI	CTGCAG	1 cut(s)	109
RsaI	GTAC	3 cut(s)	238, 324, 1176
RsaNI	GTAC	3 cut(s)	237, 323, 1175
SacI	GAGCTC	1 cut(s)	399
SapI	GCTCTTC	1 cut(s)	1225
SaqAI	TTAA	8 cut(s)	227, 353, 489, 542, 857, 962, 1049, 1377
SatI	GCNGC	2 cut(s)	672, 846
Sau3AI	GATC	4 cut(s)	459, 871, 1026, 1325
Sau96I	GGNCC	1 cut(s)	1189
ScrFI	CCNGG	2 cut(s)	64, 714
SduI	GDGCHC	3 cut(s)	304, 399, 523
SexAI	ACCWGGT	1 cut(s)	62
SfaNI	GCATC	2 cut(s)	846, 861
SfcI	CTRYAG	3 cut(s)	105, 212, 930
SfuI	TTCGAA	1 cut(s)	681
SinI	GGWCC	1 cut(s)	1189
SmlI	CTYRAG	1 cut(s)	1151
SmoI	CTYRAG	1 cut(s)	1151
SphI	GCATGC	1 cut(s)	796
Sse9I	AATT	9 cut(s)	83, 147, 228, 616, 695, 890, 1180, 1261, 1374
SsiI	CCGC	6 cut(s)	52, 185, 295, 305, 1125, 1284
SspI	AATATT	1 cut(s)	1200
SspMI	CTAG	5 cut(s)	516, 575, 587, 642, 1422
SstI	GAGCTC	1 cut(s)	399
StyD4I	CCNGG	2 cut(s)	62, 712
StyI	CCWWGG	2 cut(s)	249, 314
TaaI	ACNGT	5 cut(s)	32, 733, 886, 1061, 1386
TaqI	TCGA	5 cut(s)	126, 436, 681, 1041, 1107
TasI	AATT	9 cut(s)	83, 147, 228, 616, 695, 890, 1180, 1261, 1374
TatI	WGTACW	1 cut(s)	322
TfiI	GAWTC	6 cut(s)	208, 408, 480, 748, 992, 1350
Tru1I	TTAA	8 cut(s)	227, 353, 489, 542, 857, 962, 1049, 1377
Tru9I	TTAA	8 cut(s)	227, 353, 489, 542, 857, 962, 1049, 1377
TscAI	CASTG	2 cut(s)	304, 891
TseI	GCWGC	2 cut(s)	671, 845
TspDTI	ATGAA	4 cut(s)	105, 708, 1157, 1404
TspGWI	ACGGA	1 cut(s)	1383
TspRI	CASTG	2 cut(s)	304, 891
VpaK11BI	GGWCC	1 cut(s)	1189
XagI	CCTNNNNNAGG	1 cut(s)	371
XapI	RAATTY	2 cut(s)	616, 1180
XbaI	TCTAGA	1 cut(s)	515
XceI	RCATGY	2 cut(s)	262, 796
XcmI	CCANNNNNNNNNTGG	1 cut(s)	917
XmiI	GTMKAC	2 cut(s)	903, 1137
XspI	CTAG	5 cut(s)	516, 575, 587, 642, 1422
Zsp2I	ATGCAT	1 cut(s)	665

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.