Rorug06G0356100

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004832
Physical Location & Seq
Forward (+)
50379150 .. 50380361
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0356100.1

Sequence Viewer

Length: 612 bp
ATGAAGCCAACTACATCATTCTTCTTCCTCTCAACCCTGTGCGTGCTCCAACAAGTTGCCCTCGTCGCAAATAGTCAACCCGGCAAACCCGTCGGAGGCCAACCTGGCAAACCCGTCGGAGGCCAACCGGGAGGAACCTCCGGGCCTGTCAGTGGTAATCAAATAGAAGAAGCATGCAAATACGCGACTAACAAGGATCTGTGCATCACCAACCTCAAGAAAGACCCAAACAGCCAAGGAGCGGACCTCGTCGGCCTGGCCTTGATTGCGATCAGGCTGGCCGCCGCGAATGCCGCTGACGTGGAATCCCACCTGAGAACGTTGATGATCAACAATGCCACCTCTCTATCCCCGGAGGTTCAGCAGGGGGTTTCGGACTGTATAGAACATTACACCGACGCCAACGAGCAGCTTGACGACTCGGTGGCCGCGGTGTCGGCCAAGGAATTCAATGACGTCGAGATGTGGGTGAAGGTTGCCATTGGCGATGCGGCTTATTGTCAAAACGCCATGAAGGGTCAGCACACTGTGGTTGAACAGAAGAACAAATTGTTTATGCAGATGTGCGAAAATGTCTTGGCCATGATCAAGGCCGTGACTCAAAAGGGTTGA

Protein Analysis

203

Amino Acids

21.51

Weight (kDa)

5.35

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 54 - 195 1.4e-23 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
AccBSI CCGCTC 1 cut(s) 242
AccII CGCG 3 cut(s) 185, 287, 431
AciI CCGC 7 cut(s) 242, 282, 285, 294, 429, 431, 491
AclI AACGTT 1 cut(s) 320
AclWI GGATC 1 cut(s) 204
AcoI YGGCCR 4 cut(s) 279, 426, 438, 579
AcsI RAATTY 1 cut(s) 446
AcyI GRCGYC 2 cut(s) 399, 456
AdeI CACNNNGTG 1 cut(s) 529
AfiI CCNNNNNNNGG 4 cut(s) 95, 119, 152, 241
AgsI TTSAA 2 cut(s) 451, 536
AjiI CACGTC 1 cut(s) 301
AjnI CCWGG 2 cut(s) 103, 255
AluBI AGCT 1 cut(s) 412
AluI AGCT 1 cut(s) 412
Alw21I GWGCWC 1 cut(s) 48
AlwI GGATC 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 1 cut(s) 446
AspS9I GGNCC 2 cut(s) 143, 244
AsuC2I CCSGG 4 cut(s) 81, 129, 142, 353
AsuHPI GGTGA 2 cut(s) 199, 481
AvaII GGWCC 1 cut(s) 244
BalI TGGCCA 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 421
BceAI ACGGC 1 cut(s) 578
BcgI CGANNNNNNTGC 4 cut(s) 73, 97, 107, 131
BciT130I CCWGG 2 cut(s) 105, 257
BclI TGATCA 2 cut(s) 327, 585
BcnI CCSGG 4 cut(s) 81, 129, 142, 353
BglI GCCNNNNNGGC 1 cut(s) 105
BisI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
BlsI GCNGC 6 cut(s) 283, 286, 295, 411, 430, 493
Bme1390I CCNGG 6 cut(s) 81, 105, 129, 142, 257, 353
Bme18I GGWCC 1 cut(s) 244
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 143, 244
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 6 cut(s) 81, 105, 129, 142, 257, 353
BmsI GCATC 2 cut(s) 213, 478
BplI GAGNNNNNCTC 2 cut(s) 231, 263
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 4 cut(s) 81, 129, 142, 353
BsaBI GATNNNNATC 1 cut(s) 269
BsaHI GRCGYC 2 cut(s) 399, 456
BsaJI CCNNGG 4 cut(s) 235, 351, 429, 441
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 119, 152, 241
Bse8I GATNNNNATC 1 cut(s) 269
BseBI CCWGG 2 cut(s) 105, 257
BseDI CCNNGG 4 cut(s) 235, 351, 429, 441
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 4 cut(s) 95, 119, 152, 241
BseMII CTCAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 421
Bsh1236I CGCG 3 cut(s) 185, 287, 431
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 4 cut(s) 81, 128, 141, 353
BslI CCNNNNNNNGG 4 cut(s) 95, 119, 152, 241
BsmI GAATGC 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 4 cut(s) 196, 270, 327, 585
BspACI CCGC 7 cut(s) 242, 282, 285, 294, 429, 431, 491
BspCNI CTCAG 1 cut(s) 306
BspFNI CGCG 3 cut(s) 185, 287, 431
BspLI GGNNCC 1 cut(s) 136
BspPI GGATC 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 242
BssECI CCNNGG 4 cut(s) 235, 351, 429, 441
BssMI GATC 4 cut(s) 196, 270, 327, 585
BssNI GRCGYC 2 cut(s) 399, 456
BssT1I CCWWGG 2 cut(s) 235, 441
Bst2UI CCWGG 2 cut(s) 105, 257
Bst4CI ACNGT 2 cut(s) 380, 529
BstACI GRCGYC 2 cut(s) 399, 456
BstC8I GCNNGC 3 cut(s) 44, 175, 279
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 429
BstFNI CGCG 3 cut(s) 185, 287, 431
BstKTI GATC 4 cut(s) 199, 273, 330, 588
BstMBI GATC 4 cut(s) 196, 270, 327, 585
BstMWI GCNNNNNNNGC 6 cut(s) 65, 105, 266, 290, 293, 437
BstNI CCWGG 2 cut(s) 105, 257
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 6 cut(s) 79, 103, 127, 140, 255, 351
BstUI CGCG 3 cut(s) 185, 287, 431
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 501
BtrI CACGTC 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 157, 525
Cac8I GCNNGC 3 cut(s) 44, 175, 279
Cfr13I GGNCC 2 cut(s) 143, 244
Cfr42I CCGCGG 1 cut(s) 432
CseI GACGC 1 cut(s) 407
CviAII CATG 3 cut(s) 174, 511, 583
DdeI CTNAG 1 cut(s) 314
DpnI GATC 4 cut(s) 198, 272, 329, 587
DpnII GATC 4 cut(s) 196, 270, 327, 585
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 4 cut(s) 279, 426, 438, 579
Eco130I CCWWGG 2 cut(s) 235, 441
Eco47I GGWCC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 103, 255
EcoT14I CCWWGG 2 cut(s) 235, 441
ErhI CCWWGG 2 cut(s) 235, 441
FaeI CATG 3 cut(s) 177, 514, 586
FaiI YATR 5 cut(s) 175, 383, 512, 557, 584
FatI CATG 3 cut(s) 173, 510, 582
FbaI TGATCA 2 cut(s) 327, 585
Fnu4HI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
Fsp4HI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
GluI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
HapII CCGG 4 cut(s) 81, 128, 141, 353
HgaI GACGC 1 cut(s) 407
Hin1I GRCGYC 2 cut(s) 399, 456
Hin1II CATG 3 cut(s) 177, 514, 586
HincII GTYRAC 1 cut(s) 77
HindII GTYRAC 1 cut(s) 77
HinfI GANTC 3 cut(s) 305, 419, 598
HpaII CCGG 4 cut(s) 81, 128, 141, 353
HphI GGTGA 2 cut(s) 199, 481
Hpy166II GTNNAC 1 cut(s) 77
Hpy188I TCNGA 3 cut(s) 95, 119, 376
Hpy188III TCNNGA 2 cut(s) 217, 460
Hpy8I GTNNAC 1 cut(s) 77
Hpy99I CGWCG 6 cut(s) 68, 95, 119, 254, 401, 461
HpyAV CCTTC 2 cut(s) 466, 508
HpyCH4III ACNGT 2 cut(s) 380, 529
HpyCH4IV ACGT 3 cut(s) 300, 320, 456
HpyCH4V TGCA 3 cut(s) 177, 204, 559
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 105, 266, 290, 293, 437
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 3 cut(s) 300, 320, 456
Hsp92I GRCGYC 2 cut(s) 399, 456
Hsp92II CATG 3 cut(s) 177, 514, 586
Ksp22I TGATCA 2 cut(s) 327, 585
KspI CCGCGG 1 cut(s) 432
Kzo9I GATC 4 cut(s) 196, 270, 327, 585
LmnI GCTCC 2 cut(s) 51, 239
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 2 cut(s) 213, 478
MaeII ACGT 3 cut(s) 300, 320, 456
MaeIII GTNAC 1 cut(s) 595
MalI GATC 4 cut(s) 198, 272, 329, 587
MbiI CCGCTC 1 cut(s) 242
MboI GATC 4 cut(s) 196, 270, 327, 585
MboII GAAGA 4 cut(s) 13, 16, 179, 553
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 48
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 2 cut(s) 446, 548
MluNI TGGCCA 1 cut(s) 581
MlyI GAGTC 2 cut(s) 413, 592
MmeI TCCRAC 3 cut(s) 73, 73, 97
Mox20I TGGCCA 1 cut(s) 581
MscI TGGCCA 1 cut(s) 581
Msp20I TGGCCA 1 cut(s) 581
MspA1I CMGCKG 2 cut(s) 296, 431
MspI CCGG 4 cut(s) 81, 128, 141, 353
MspR9I CCNGG 6 cut(s) 81, 105, 129, 142, 257, 353
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 2 cut(s) 105, 257
MvnI CGCG 3 cut(s) 185, 287, 431
MwoI GCNNNNNNNGC 6 cut(s) 65, 105, 266, 290, 293, 437
NciI CCSGG 4 cut(s) 81, 129, 142, 353
NdeII GATC 4 cut(s) 196, 270, 327, 585
NlaIII CATG 3 cut(s) 177, 514, 586
NlaIV GGNNCC 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 1 cut(s) 177
PaeI GCATGC 1 cut(s) 177
PctI GAATGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 305
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 6 cut(s) 283, 286, 295, 411, 430, 493
PleI GAGTC 2 cut(s) 413, 592
PpsI GAGTC 2 cut(s) 413, 592
Psp1406I AACGTT 1 cut(s) 320
Psp6I CCWGG 2 cut(s) 103, 255
PspGI CCWGG 2 cut(s) 103, 255
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 2 cut(s) 143, 244
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 248
SacII CCGCGG 1 cut(s) 432
SatI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
Sau3AI GATC 4 cut(s) 196, 270, 327, 585
Sau96I GGNCC 2 cut(s) 143, 244
SchI GAGTC 2 cut(s) 413, 592
ScrFI CCNGG 6 cut(s) 81, 105, 129, 142, 257, 353
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 2 cut(s) 213, 478
Sfr303I CCGCGG 1 cut(s) 432
SgrBI CCGCGG 1 cut(s) 432
SinI GGWCC 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SphI GCATGC 1 cut(s) 177
Sse9I AATT 2 cut(s) 446, 548
SsiI CCGC 7 cut(s) 242, 282, 285, 294, 429, 431, 491
StyD4I CCNGG 6 cut(s) 79, 103, 127, 140, 255, 351
StyI CCWWGG 2 cut(s) 235, 441
TaaI ACNGT 2 cut(s) 380, 529
TaiI ACGT 3 cut(s) 303, 323, 459
TaqI TCGA 1 cut(s) 459
TasI AATT 2 cut(s) 446, 548
TauI GCSGC 5 cut(s) 284, 287, 296, 431, 494
TfiI GAWTC 1 cut(s) 305
TscAI CASTG 2 cut(s) 157, 532
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 2 cut(s) 17, 527
TspRI CASTG 2 cut(s) 157, 532
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 177
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.