Rorug06G0357100

polyadenylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006995
Physical Location & Seq
Reverse (-)
50461547 .. 50462258
712 bp
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UTR
Exon/CDS
Intron
Rorug06G0357100.1

Sequence Viewer

Length: 471 bp
ATGGTGGTTACGGTCTCTGCAGAACCCGAGTACAAGAAACCCAAACTAGAAAAGGAGAACTTACTATCAACCATTGTGGGCATTCAAGGCCTTGTGTATTGCAAATCAGGCCCTAAAGTCACTCCCCTTGAAGGATCGGTGGCCAGGATAACATGTGAAGCTGTAGATGAATATGGGTTCGAAACTGCACCAATCACTATTTTGAGTGATGCAACTGATGCAAAGGGTTACTTCCTTGCAACAATATCTCCATTTGAGATTCAAAACAAGAAGAAGAAGCTGACACAGTGCAAAGCTTTTCTTGAACTCTCTCCACTAGATTCTTGCAACGTTCTCACAGATGCAAACAATGGAATCAGTGGCGCTCTACTCACTTCATATCACCTCCTCCATGAGAAGAACATGAAGTTATTTACTGTAGGGCCTTTCATTTTCGCATCAGAATCTAAACCGGTCGATAATGGTTACTAG

Protein Analysis

156

Amino Acids

17.0

Weight (kDa)

5.71

Isoelectric Point (pI)

18.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 28 - 121 5.4e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 330
AclWI GGATC 1 cut(s) 142
AcoI YGGCCR 1 cut(s) 141
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 152
AgeI ACCGGT 1 cut(s) 451
AgsI TTSAA 4 cut(s) 86, 131, 263, 305
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 3 cut(s) 161, 280, 296
AluI AGCT 3 cut(s) 161, 280, 296
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 4 cut(s) 88, 109, 141, 422
AsiGI ACCGGT 1 cut(s) 451
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 110, 422
AsuHPI GGTGA 1 cut(s) 374
AsuII TTCGAA 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 26
BalI TGGCCA 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 3 cut(s) 47, 317, 469
BfmI CTRYAG 3 cut(s) 18, 162, 417
BfoI RGCGCY 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 110, 422
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 4 cut(s) 199, 208, 331, 446
Bpu14I TTCGAA 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 19
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse118I RCCGGY 1 cut(s) 451
BseBI CCWGG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 456
BshFI GGCC 4 cut(s) 90, 111, 143, 424
BshTI ACCGGT 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 456
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 81
BsnI GGCC 4 cut(s) 90, 111, 143, 424
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 26
Bsp119I TTCGAA 1 cut(s) 180
Bsp143I GATC 1 cut(s) 134
BspANI GGCC 4 cut(s) 90, 111, 143, 424
BspMAI CTGCAG 1 cut(s) 22
BspPI GGATC 1 cut(s) 142
BspT104I TTCGAA 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 451
BssMI GATC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 3 cut(s) 13, 288, 418
BstAPI GCANNNNNTGC 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 134
BstMCI CGRYCG 1 cut(s) 456
BstMWI GCNNNNNNNGC 3 cut(s) 87, 108, 218
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 143
BstSFI CTRYAG 3 cut(s) 18, 162, 417
BsuRI GGCC 4 cut(s) 90, 111, 143, 424
BtsIMutI CAGTG 2 cut(s) 293, 364
CfoI GCGC 1 cut(s) 365
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 110, 422
Csp6I GTAC 1 cut(s) 31
CspAI ACCGGT 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 3 cut(s) 153, 392, 403
CviJI RGCY 7 cut(s) 90, 111, 143, 161, 280, 296, 424
CviKI_1 RGCY 7 cut(s) 90, 111, 143, 161, 280, 296, 424
CviQI GTAC 1 cut(s) 31
DpnI GATC 1 cut(s) 136
DpnII GATC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 90
Eco31I GGTCTC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 110, 422
EcoRII CCWGG 1 cut(s) 143
FaeI CATG 3 cut(s) 156, 395, 406
FaiI YATR 5 cut(s) 154, 174, 379, 393, 404
FalI AAGNNNNNCTT 6 cut(s) 44, 76, 215, 247, 285, 317
FatI CATG 3 cut(s) 152, 391, 402
FspBI CTAG 3 cut(s) 47, 317, 469
GlaI GCGC 1 cut(s) 364
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 4 cut(s) 90, 111, 143, 424
HapII CCGG 1 cut(s) 452
HhaI GCGC 1 cut(s) 365
Hin1II CATG 3 cut(s) 156, 395, 406
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HindIII AAGCTT 1 cut(s) 294
HinfI GANTC 4 cut(s) 259, 320, 354, 443
HpaII CCGG 1 cut(s) 452
HphI GGTGA 1 cut(s) 374
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 3 cut(s) 13, 288, 418
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 9 cut(s) 20, 102, 188, 212, 221, 239, 291, 327, 344
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 108, 218
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 3 cut(s) 156, 395, 406
HspAI GCGC 1 cut(s) 363
Kzo9I GATC 1 cut(s) 134
LpnPI CCDG 4 cut(s) 93, 130, 157, 465
LweI GCATC 4 cut(s) 199, 208, 331, 446
MaeI CTAG 3 cut(s) 47, 317, 469
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 4 cut(s) 7, 118, 227, 464
MalI GATC 1 cut(s) 136
MboI GATC 1 cut(s) 134
MboII GAAGA 3 cut(s) 283, 286, 409
MlsI TGGCCA 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 143
MnlI CCTC 2 cut(s) 395, 398
Mox20I TGGCCA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 143
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 81
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 87, 108, 218
NdeII GATC 1 cut(s) 134
NlaIII CATG 3 cut(s) 156, 395, 406
NmuCI GTSAC 1 cut(s) 118
NspI RCATGY 1 cut(s) 156
NspV TTCGAA 1 cut(s) 180
PceI AGGCCT 1 cut(s) 90
PciI ACATGT 1 cut(s) 152
PctI GAATGC 1 cut(s) 81
PfeI GAWTC 4 cut(s) 259, 320, 354, 443
PinAI ACCGGT 1 cut(s) 451
PscI ACATGT 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspPI GGNCC 2 cut(s) 110, 422
PstI CTGCAG 1 cut(s) 22
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
Sau3AI GATC 1 cut(s) 134
Sau96I GGNCC 2 cut(s) 110, 422
ScrFI CCNGG 1 cut(s) 145
SetI ASST 5 cut(s) 163, 282, 298, 333, 387
SfaNI GCATC 4 cut(s) 199, 208, 331, 446
SfcI CTRYAG 3 cut(s) 18, 162, 417
SfuI TTCGAA 1 cut(s) 180
SseBI AGGCCT 1 cut(s) 90
SspMI CTAG 3 cut(s) 47, 317, 469
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 143
TaaI ACNGT 3 cut(s) 13, 288, 418
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 180, 456
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 4 cut(s) 259, 320, 354, 443
TscAI CASTG 2 cut(s) 293, 364
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 4 cut(s) 183, 366, 418, 419
TspRI CASTG 2 cut(s) 293, 364
XceI RCATGY 1 cut(s) 156
XspI CTAG 3 cut(s) 47, 317, 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.