Rorug06G0399400

Polyadenylate-binding protein-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004663
Physical Location & Seq
Forward (+)
54433504 .. 54438641
5138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0399400.1

Sequence Viewer

Length: 1845 bp
ATGAAGTTTATGAAGTTAGGGTCCAAGCCTGATGCGTTTCAAGCTGATGGCAAGTTGATCAGATATGTAACATCTGAGTTGGATTCAGATGTCATCGTAAAGGTTGGGGAAGTGCAATTTTACCTTCACAAGTTCCCTCTCCTTTCCAAGAGTAATCGCTTGCAAAAACTAGTACCTAAAGCCAATGAGGAGAAAGTCGTTGAAGTTTGCATGGATGATTTCCCCGGTGGGGCAAATTCCTTTGAAGTCTGTGCAAAATTTTGCTATGGAATGACTGTTACTCTCAATGCTTACAATGTTGTGGCTGCTCGTTGTGCCGCTGAGTATCTGGAGATGACCGAGGATGTTGATCGCAGTAACCTGATATATAAAATTGAAGTATTCCTGAATTCAAGTATATTTTGTAGCTGGAAAGATACCATTATTGTTCTCCAAACCACCAAACCTCTTCTGTCATGGTCCAAGGATCTGAAGATTGTCGGAAGATGCATAGATTCAATCGCTTCTAAAACTTCTGTCGACCCTGCAAATATCAGCTGGTCCTACACATACAACCGAAAATTAACAGAGCCTGATAAAATTATTGAAGAAGGGATTAATTATCGAGACAAAATGGAAAATATTCCAAGGGATTGGTGGGTTGAAGATATATGTGAGCTAGATATTGATCTCTACAGGCGTGTCATGATTGCTGTGAAATCAAAAGGAAGAATGGATGGTGCCGTTATTGGGGAGGCTCTGAAAACTTATGCACTTAGATGGTTGCCAGATTCTGTTGATGCGTTGATTTCTGATGATCATGCATTGAGAAACAAATCTCTGGTAGAAACTATTATTTGTTTATTGCCATCTGACAAAGGTGTGGGTTGTTCATGCAGTTTCTTACTGAAACTTTTGAAAGTTAGTACTTTGGTTGGAGCAGATGAGTCATTGAGGGAGGTTCTGGTTAACAGGATTGGTTTGAAGTTACCTGAAGCTCGTGTTGGTGATTTATTGATCCCTGCTCGGTCTCCTCAAATTACTATTTATGATGTTGAATTGGTTCAGTCCATTGTGAATCGGTTTGTGATACATGAAAAGAGTAACTGGGATTTAGATGTTGTTCGGAAGAATGAAAGGGGTACTGATAATTTTATCTTGGGGCATGGATCCTTGGTGGGTGTTGGTAGACTGATTGATGGGTATCTTGCCGAAATTGCTCATGACCCTAATCTTAACCTTGGTAGTTTCATAGACTTGTCACAATTGCTTCCTGAGTTAGCGAGGCCAATTCATGATGGATTGTACAAAGCCATTGATATCTACCTGAAGGAGCACCGTAGTCTGACAAAGGCTGAAAGGAAAAGAATATGTGGATTAATGGATGTCAAGAAACTGACAATGGAGGCATCCATGGATGCTGCACAGAACGAGCAGCTTCCACTGCGAGTTGTTGTCCAAGTTCTCTTTTTTGAGCAGGTGAGATCTTCAGCTGCTGCTCGGTCCCTTAACAACCATCCATCCGATGCTTCACATTCCACCACAAATTTGGATGAAGAATGTGACAAGACAGCAGAAGAAAACTGCAAACCTTTAGGAAAACAGCTGAGTCAAATGAAGGTAAGCGAAGAGTTGCAGAAGAATGGGAAATTGGGTAAGAAGAACAGCAAGAATAGTAGAAGTGGTGCACAGTTGTTGCCGTCTAGGTCAAGGAGGATATTTGACAAGTTATGGTCTGTGGGGAAGGGGGGGCATGGAGAGAACAAGAGCTCGGAGACTTCTGGGAGTTCTCAGAGCCCAACTTCTATAGTTCCTGGTGATTCCAAGTCTGTTTCATCAAGACACAGGAGGCATTCTATATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

614

Amino Acids

68.64

Weight (kDa)

8.08

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 211 - 464 8.5e-83 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1447
Acc36I ACCTGC 1 cut(s) 1447
AccB1I GGYRCC 1 cut(s) 719
AccI GTMKAC 2 cut(s) 519, 1168
AciI CCGC 1 cut(s) 318
AclWI GGATC 4 cut(s) 474, 991, 1143, 1156
AcsI RAATTY 4 cut(s) 235, 257, 388, 1525
AcuI CTGAAG 4 cut(s) 491, 993, 1328, 1452
AfaI GTAC 4 cut(s) 174, 907, 1123, 1286
AfiI CCNNNNNNNGG 2 cut(s) 229, 729
AhlI ACTAGT 1 cut(s) 169
AjnI CCWGG 1 cut(s) 1792
AjuI GAANNNNNNNTTGG 4 cut(s) 966, 998, 1613, 1645
AluBI AGCT 9 cut(s) 44, 408, 537, 658, 977, 1417, 1472, 1585, 1749
AluI AGCT 9 cut(s) 44, 408, 537, 658, 977, 1417, 1472, 1585, 1749
Alw21I GWGCWC 3 cut(s) 1317, 1669, 1751
Alw26I GTCTC 3 cut(s) 600, 1014, 1748
Alw44I GTGCAC 1 cut(s) 1665
AlwI GGATC 4 cut(s) 474, 991, 1143, 1156
AlwNI CAGNNNCTG 3 cut(s) 572, 773, 1475
AoxI GGCC 1 cut(s) 1265
ApaLI GTGCAC 1 cut(s) 1665
ApeKI GCWGC 5 cut(s) 305, 1400, 1414, 1472, 1475
ApoI RAATTY 4 cut(s) 235, 257, 388, 1525
AseI ATTAAT 2 cut(s) 597, 1358
Asp700I GAANNNNTTC 2 cut(s) 621, 1041
AspS9I GGNCC 4 cut(s) 21, 459, 540, 1482
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 3 cut(s) 998, 1471, 1808
AvaII GGWCC 4 cut(s) 21, 459, 540, 1482
BaeGI GKGCMC 1 cut(s) 1669
BamHI GGATCC 1 cut(s) 1148
BanI GGYRCC 1 cut(s) 719
BanII GRGCYC 2 cut(s) 1751, 1778
BauI CACGAG 1 cut(s) 978
Bbv12I GWGCWC 3 cut(s) 1317, 1669, 1751
BbvI GCAGC 5 cut(s) 292, 1387, 1426, 1459, 1462
BccI CCATC 8 cut(s) 41, 710, 753, 856, 1172, 1271, 1503, 1507
BceAI ACGGC 2 cut(s) 707, 1663
BciT130I CCWGG 1 cut(s) 1794
BclI TGATCA 2 cut(s) 57, 796
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 3 cut(s) 600, 1014, 1748
BcuI ACTAGT 1 cut(s) 169
BfaI CTAG 4 cut(s) 170, 659, 1683, 1843
BfmI CTRYAG 2 cut(s) 673, 1785
BfuAI ACCTGC 1 cut(s) 1447
BglII AGATCT 1 cut(s) 1463
BisI GCNGC 6 cut(s) 306, 318, 1401, 1415, 1473, 1476
BlsI GCNGC 6 cut(s) 307, 319, 1402, 1416, 1474, 1477
BmcAI AGTACT 1 cut(s) 907
Bme1390I CCNGG 2 cut(s) 225, 1794
Bme18I GGWCC 4 cut(s) 21, 459, 540, 1482
BmgT120I GGNCC 4 cut(s) 21, 459, 540, 1482
BmiI GGNNCC 4 cut(s) 22, 721, 1150, 1484
BmrFI CCNGG 2 cut(s) 225, 1794
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 6 cut(s) 22, 476, 769, 1387, 1397, 1495
BmuI ACTGGG 1 cut(s) 1096
BpmI CTGGAG 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 225
BsaBI GATNNNNATC 3 cut(s) 348, 666, 1182
BsaI GGTCTC 1 cut(s) 1014
BsaJI CCNNGG 7 cut(s) 223, 339, 462, 626, 1152, 1219, 1392
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 729
Bse1I ACTGG 1 cut(s) 1091
Bse8I GATNNNNATC 3 cut(s) 348, 666, 1182
BseBI CCWGG 1 cut(s) 1794
BseDI CCNNGG 7 cut(s) 223, 339, 462, 626, 1152, 1219, 1392
BseGI GGATG 9 cut(s) 220, 349, 721, 1369, 1388, 1402, 1495, 1499, 1537
BseJI GATNNNNATC 3 cut(s) 348, 666, 1182
BseLI CCNNNNNNNGG 2 cut(s) 229, 729
BseMII CTCAG 5 cut(s) 66, 312, 1245, 1577, 1784
BseNI ACTGG 1 cut(s) 1091
BseRI GAGGAG 2 cut(s) 203, 1002
BseSI GKGCMC 1 cut(s) 1669
BseXI GCAGC 5 cut(s) 292, 1387, 1426, 1459, 1462
BsgI GTGCAG 1 cut(s) 1386
BshFI GGCC 1 cut(s) 1267
BshNI GGYRCC 1 cut(s) 719
BsiHKAI GWGCWC 3 cut(s) 1317, 1669, 1751
BsiSI CCGG 1 cut(s) 225
BslFI GGGAC 1 cut(s) 1468
BslI CCNNNNNNNGG 2 cut(s) 229, 729
BsmAI GTCTC 3 cut(s) 600, 1014, 1748
BsmFI GGGAC 1 cut(s) 1468
BsmI GAATGC 1 cut(s) 1831
BsnI GGCC 1 cut(s) 1267
Bso31I GGTCTC 1 cut(s) 1014
Bsp1286I GDGCHC 4 cut(s) 1317, 1669, 1751, 1778
Bsp1407I TGTACA 1 cut(s) 1284
Bsp143I GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
Bsp19I CCATGG 1 cut(s) 1392
BspACI CCGC 1 cut(s) 318
BspANI GGCC 1 cut(s) 1267
BspCNI CTCAG 5 cut(s) 67, 313, 1246, 1578, 1783
BspHI TCATGA 3 cut(s) 684, 1201, 1273
BspLI GGNNCC 4 cut(s) 22, 721, 1150, 1484
BspMI ACCTGC 1 cut(s) 1447
BspPI GGATC 4 cut(s) 474, 991, 1143, 1156
BspT107I GGYRCC 1 cut(s) 719
BspTNI GGTCTC 1 cut(s) 1014
BsrGI TGTACA 1 cut(s) 1284
BsrI ACTGG 1 cut(s) 1091
BssECI CCNNGG 7 cut(s) 223, 339, 462, 626, 1152, 1219, 1392
BssMI GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
BssSI CACGAG 1 cut(s) 978
BssT1I CCWWGG 5 cut(s) 462, 626, 1152, 1219, 1392
Bst2BI CACGAG 1 cut(s) 978
Bst2UI CCWGG 1 cut(s) 1794
Bst4CI ACNGT 3 cut(s) 277, 1319, 1671
Bst6I CTCTTC 2 cut(s) 453, 1602
BstAUI TGTACA 1 cut(s) 1284
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 6 cut(s) 75, 321, 755, 1254, 1586, 1770
BstDSI CCRYGG 1 cut(s) 1392
BstF5I GGATG 9 cut(s) 220, 349, 721, 1369, 1388, 1402, 1495, 1499, 1537
BstKTI GATC 8 cut(s) 60, 352, 469, 670, 799, 999, 1151, 1466
BstMAI GTCTC 3 cut(s) 600, 1014, 1748
BstMBI GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
BstMWI GCNNNNNNNGC 4 cut(s) 41, 314, 1196, 1423
BstNI CCWGG 1 cut(s) 1794
BstSCI CCNGG 2 cut(s) 223, 1792
BstSFI CTRYAG 2 cut(s) 673, 1785
BstSLI GKGCMC 1 cut(s) 1669
BstV1I GCAGC 5 cut(s) 292, 1387, 1426, 1459, 1462
BstX2I RGATCY 3 cut(s) 466, 1148, 1463
BstXI CCANNNNNNTGG 2 cut(s) 633, 1528
BstYI RGATCY 3 cut(s) 466, 1148, 1463
BsuRI GGCC 1 cut(s) 1267
BtgI CCRYGG 1 cut(s) 1392
BtsCI GGATG 9 cut(s) 220, 349, 721, 1369, 1388, 1402, 1495, 1499, 1537
BtsI GCAGTG 1 cut(s) 1421
BtsIMutI CAGTG 1 cut(s) 1421
BveI ACCTGC 1 cut(s) 1447
Cac8I GCNNGC 1 cut(s) 161
CaiI CAGNNNCTG 3 cut(s) 572, 773, 1475
CciI TCATGA 3 cut(s) 684, 1201, 1273
Cfr13I GGNCC 4 cut(s) 21, 459, 540, 1482
Csp6I GTAC 4 cut(s) 173, 906, 1122, 1285
CviQI GTAC 4 cut(s) 173, 906, 1122, 1285
DdeI CTNAG 6 cut(s) 75, 321, 755, 1254, 1586, 1770
DpnI GATC 8 cut(s) 59, 351, 468, 669, 798, 998, 1150, 1465
DpnII GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
Eam1104I CTCTTC 2 cut(s) 453, 1602
EarI CTCTTC 2 cut(s) 453, 1602
Ecl136II GAGCTC 1 cut(s) 1749
Eco130I CCWWGG 5 cut(s) 462, 626, 1152, 1219, 1392
Eco24I GRGCYC 2 cut(s) 1751, 1778
Eco31I GGTCTC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 1300
Eco47I GGWCC 4 cut(s) 21, 459, 540, 1482
Eco53kI GAGCTC 1 cut(s) 1749
Eco57I CTGAAG 4 cut(s) 491, 993, 1328, 1452
EcoICRI GAGCTC 1 cut(s) 1749
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 1792
EcoRV GATATC 1 cut(s) 1300
EcoT14I CCWWGG 5 cut(s) 462, 626, 1152, 1219, 1392
EcoT22I ATGCAT 2 cut(s) 491, 805
EcoT38I GRGCYC 2 cut(s) 1751, 1778
ErhI CCWWGG 5 cut(s) 462, 626, 1152, 1219, 1392
FaqI GGGAC 1 cut(s) 1468
FbaI TGATCA 2 cut(s) 57, 796
FblI GTMKAC 2 cut(s) 519, 1168
Fnu4HI GCNGC 6 cut(s) 306, 318, 1401, 1415, 1473, 1476
FokI GGATG 9 cut(s) 227, 356, 728, 1375, 1376, 1409, 1482, 1486, 1544
FriOI GRGCYC 2 cut(s) 1751, 1778
Fsp4HI GCNGC 6 cut(s) 306, 318, 1401, 1415, 1473, 1476
FspBI CTAG 4 cut(s) 170, 659, 1683, 1843
GluI GCNGC 6 cut(s) 306, 318, 1401, 1415, 1473, 1476
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 1 cut(s) 1267
HapII CCGG 1 cut(s) 225
HincII GTYRAC 2 cut(s) 520, 949
HindII GTYRAC 2 cut(s) 520, 949
HinfI GANTC 7 cut(s) 83, 494, 770, 926, 1057, 1588, 1799
HpaI GTTAAC 1 cut(s) 949
HpaII CCGG 1 cut(s) 225
HphI GGTGA 3 cut(s) 998, 1471, 1808
Hpy166II GTNNAC 4 cut(s) 520, 949, 1169, 1667
Hpy188III TCNNGA 9 cut(s) 329, 385, 605, 685, 1202, 1253, 1274, 1369, 1818
Hpy8I GTNNAC 4 cut(s) 520, 949, 1169, 1667
HpyAV CCTTC 5 cut(s) 134, 584, 1303, 1591, 1717
HpyCH4III ACNGT 3 cut(s) 277, 1319, 1671
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 314, 1196, 1423
HpyF3I CTNAG 6 cut(s) 75, 321, 755, 1254, 1586, 1770
Ksp22I TGATCA 2 cut(s) 57, 796
KspAI GTTAAC 1 cut(s) 949
Kzo9I GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
LmnI GCTCC 2 cut(s) 917, 1312
Lsp1109I GCAGC 5 cut(s) 292, 1387, 1426, 1459, 1462
LweI GCATC 6 cut(s) 22, 476, 769, 1387, 1397, 1495
MaeI CTAG 4 cut(s) 170, 659, 1683, 1843
MaeIII GTNAC 7 cut(s) 67, 277, 356, 966, 1082, 1239, 1541
MalI GATC 8 cut(s) 59, 351, 468, 669, 798, 998, 1150, 1465
MboI GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
MfeI CAATTG 1 cut(s) 1244
MflI RGATCY 3 cut(s) 466, 1148, 1463
MhlI GDGCHC 4 cut(s) 1317, 1669, 1751, 1778
MlyI GAGTC 2 cut(s) 935, 1597
MmeI TCCRAC 3 cut(s) 60, 460, 895
Mph1103I ATGCAT 2 cut(s) 491, 805
MroXI GAANNNNTTC 2 cut(s) 621, 1041
MseI TTAA 6 cut(s) 563, 597, 948, 1215, 1358, 1488
MslI CAYNNNNRTG 1 cut(s) 757
MspA1I CMGCKG 4 cut(s) 320, 537, 1472, 1585
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 2 cut(s) 225, 1794
MunI CAATTG 1 cut(s) 1244
Mva1269I GAATGC 1 cut(s) 1831
MvaI CCWGG 1 cut(s) 1794
MwoI GCNNNNNNNGC 4 cut(s) 41, 314, 1196, 1423
NciI CCSGG 1 cut(s) 225
NcoI CCATGG 1 cut(s) 1392
NdeII GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
NlaIV GGNNCC 4 cut(s) 22, 721, 1150, 1484
NmuCI GTSAC 2 cut(s) 1239, 1541
NsiI ATGCAT 2 cut(s) 491, 805
PagI TCATGA 3 cut(s) 684, 1201, 1273
PaqCI CACCTGC 1 cut(s) 1447
PctI GAATGC 1 cut(s) 1831
PdmI GAANNNNTTC 2 cut(s) 621, 1041
PfeI GAWTC 5 cut(s) 83, 494, 770, 1057, 1799
PkrI GCNGC 6 cut(s) 307, 319, 1402, 1416, 1474, 1477
PleI GAGTC 2 cut(s) 934, 1596
PpsI GAGTC 2 cut(s) 934, 1596
PshBI ATTAAT 2 cut(s) 597, 1358
Psp124BI GAGCTC 1 cut(s) 1751
Psp6I CCWGG 1 cut(s) 1792
PspGI CCWGG 1 cut(s) 1792
PspN4I GGNNCC 4 cut(s) 22, 721, 1150, 1484
PspPI GGNCC 4 cut(s) 21, 459, 540, 1482
PstNI CAGNNNCTG 3 cut(s) 572, 773, 1475
PsuI RGATCY 3 cut(s) 466, 1148, 1463
PvuII CAGCTG 3 cut(s) 537, 1472, 1585
RsaI GTAC 4 cut(s) 174, 907, 1123, 1286
RsaNI GTAC 4 cut(s) 173, 906, 1122, 1285
RseI CAYNNNNRTG 1 cut(s) 757
SacI GAGCTC 1 cut(s) 1751
SalI GTCGAC 1 cut(s) 518
SaqAI TTAA 6 cut(s) 563, 597, 948, 1215, 1358, 1488
SatI GCNGC 6 cut(s) 306, 318, 1401, 1415, 1473, 1476
Sau3AI GATC 8 cut(s) 57, 349, 466, 667, 796, 996, 1148, 1463
Sau96I GGNCC 4 cut(s) 21, 459, 540, 1482
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 2 cut(s) 935, 1597
ScrFI CCNGG 2 cut(s) 225, 1794
SduI GDGCHC 4 cut(s) 1317, 1669, 1751, 1778
SfaNI GCATC 6 cut(s) 22, 476, 769, 1387, 1397, 1495
SfcI CTRYAG 2 cut(s) 673, 1785
SinI GGWCC 4 cut(s) 21, 459, 540, 1482
SmiMI CAYNNNNRTG 1 cut(s) 757
SpeI ACTAGT 1 cut(s) 169
SsiI CCGC 1 cut(s) 318
SspI AATATT 1 cut(s) 622
SspMI CTAG 4 cut(s) 170, 659, 1683, 1843
SstI GAGCTC 1 cut(s) 1751
StyD4I CCNGG 2 cut(s) 223, 1792
StyI CCWWGG 5 cut(s) 462, 626, 1152, 1219, 1392
TaaI ACNGT 3 cut(s) 277, 1319, 1671
TaqI TCGA 2 cut(s) 519, 604
TaqII GACCGA 3 cut(s) 353, 996, 1470
TatI WGTACW 2 cut(s) 905, 1284
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 5 cut(s) 83, 494, 770, 1057, 1799
Tru1I TTAA 6 cut(s) 563, 597, 948, 1215, 1358, 1488
Tru9I TTAA 6 cut(s) 563, 597, 948, 1215, 1358, 1488
TscAI CASTG 1 cut(s) 1428
TseFI GTSAC 2 cut(s) 1239, 1541
TseI GCWGC 5 cut(s) 305, 1400, 1414, 1472, 1475
Tsp45I GTSAC 2 cut(s) 1239, 1541
TspRI CASTG 1 cut(s) 1428
VneI GTGCAC 1 cut(s) 1665
VpaK11BI GGWCC 4 cut(s) 21, 459, 540, 1482
VspI ATTAAT 2 cut(s) 597, 1358
XapI RAATTY 4 cut(s) 235, 257, 388, 1525
XcmI CCANNNNNNNNNTGG 2 cut(s) 633, 1525
XmiI GTMKAC 2 cut(s) 519, 1168
XmnI GAANNNNTTC 2 cut(s) 621, 1041
XspI CTAG 4 cut(s) 170, 659, 1683, 1843
ZrmI AGTACT 1 cut(s) 907
Zsp2I ATGCAT 2 cut(s) 491, 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.