Rorug06G0399500

Polyadenylate-binding protein-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004663
Physical Location & Seq
Forward (+)
54438816 .. 54441604
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0399500.1

Sequence Viewer

Length: 1596 bp
ATGATGATATATACACAGTTACGCAGTCGAGACAAGAGAAAGATGAAAGTCATTCACAGTCGCATTATCGTCGTCATCGTTTTGGTTTTAGTCAACCTTTCTTTGCTACCCGTCTCTGAATGTTCAGGTTCGGCGAAATGCAAAGCATGGCTGGTCCAATCAATCCCCACCGACATGCCGGGTCTCCGTCGTGTCCCTGGTGTCCTCTCCACCGCGGATGTGTTCCGGTGGCTGGCGCGGAACTCATCACAGAGACTGGACATAATTGCTCAGTACTGGCAGTTTGTAGCCAATCCTAAAGACCCTCAATCTGGAGATTTTGGATACTCAAAACAGGACATGAAGAGATTTGGAGCTCACCAAGGTGCTTCTGTTTATGGCGCCATAGATGATGCTGCTAATCGCAATCTTACTATCAGGCTGCTATCACACTCGGGTGTATATCCTGACTACACCAAAGAGCCTTCCAAACTTGCTTCAGGAAGGCCCAATGTGAAGAATGTCACTTTGTTACTTAGTGAATGGTGGGGTTCTGGCATAGTCCATGCCAAAGTTTGGATATCAGATCGGCGAGATGTGTATATTGGGTCTGCAAACAATGACTGGAAATCTCTCACACAAGTCAAGGAAGTTGGAATTTATCTTGCTGGTTGTCCAAAAATAGCGAGAAAGGTTGAGACCTACTTTGACAACCTGTGGAAACTTGCATCTCTTAATTCTACAGCTTACACAACAAATGTATCAGATAAACAGTGGCAGACCGAGAGACAAGTTCCTTGCTGGTCGCATTTTGTGGATTCTAAAGCAAGGTGTAGTTCACCTCTCCCTGGGATTGTGGAGACTGACCATGTAGCAGGATACCCCATACTGTCAGACCCTCATATGTTCAAAATGCAGCTCCAGACTCCTGGACATAATGAGTCAAGTTTGCAACCCCAATCCAGCTATCTATCTTTTGCTCCTCCAGAGCTGTCATTTGGCAAGTACCAGGCAGATGAACAAGCATGGATAGAGACTATTAAATCTGTAGGAAGTGGAGGGACTGTCAGGATTAGTACCATGGACTGGCTTGGTCAGTCCCAGTTTATGAAGCATACAATTTATTGGGCATCCCTCTCCTGTGCAATATCAGAGGTTGCGTTTGCAAAGAATGCTACAGTGAAATTATTGGTAGCACACTGGGCACATTTTATCAACAGCACAGATCAATACCTGAAATCTCTCCTCTACTCTAATGTTCTCTGCAATTCATCTGAGTATAACCACTGTTCTGGGAAAGTTGAGATCAAGTATTATATGGTCCCAGGTTTCAACTCGACAGGACCTGCCAGAACTGGCAATGGTACTCATACTGGAAATATATACCCTGGTTACACCAGGGTTAACCATGGCAAGTATGCAGTAAGCGATGTTCGAGCCCATATTGGGACGAGCAATCTTGTGTGGGATTACTTTTATACAACAGCGGGTGTCAGCTTTGGTACAAATAACTCTGCCATTGTTTCACAACTTCAACAAGTCTTTGATGCTGACTGGGATTCGGAATATGCTGTGCCAGTTGATGAGTTGGGGGAAGGTAATGCTTTTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

531

Amino Acids

59.18

Weight (kDa)

8.78

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLDc_2 PF13091 123 - 233 2.3e-10 PLD-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1333
AccB1I GGYRCC 1 cut(s) 380
AccB7I CCANNNNNTGG 2 cut(s) 555, 1065
AccII CGCG 2 cut(s) 215, 238
AciI CCGC 4 cut(s) 213, 215, 238, 1466
AcsI RAATTY 1 cut(s) 636
AcuI CTGAAG 1 cut(s) 462
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 5 cut(s) 275, 986, 1057, 1345, 1483
AfiI CCNNNNNNNGG 6 cut(s) 232, 311, 555, 827, 1065, 1425
AgsI TTSAA 4 cut(s) 889, 1312, 1514, 1590
AjnI CCWGG 7 cut(s) 196, 826, 907, 987, 1303, 1366, 1376
AleI CACNNNNGTG 2 cut(s) 363, 435
AluBI AGCT 7 cut(s) 356, 725, 898, 945, 970, 1476, 1593
AluI AGCT 7 cut(s) 356, 725, 898, 945, 970, 1476, 1593
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 8 cut(s) 24, 118, 188, 247, 671, 760, 833, 1007
AlwNI CAGNNNCTG 2 cut(s) 256, 1325
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 395, 421, 895
ApoI RAATTY 1 cut(s) 636
AspLEI GCGC 2 cut(s) 238, 383
AspS9I GGNCC 4 cut(s) 154, 486, 1300, 1322
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 350, 810
AvaI CYCGRG 1 cut(s) 433
AvaII GGWCC 3 cut(s) 154, 1300, 1322
BaeGI GKGCMC 1 cut(s) 1186
BaeI ACNNNNGTAYC 2 cut(s) 723, 756
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 2 cut(s) 358, 1420
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 382, 408, 907
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 7 cut(s) 198, 828, 909, 989, 1305, 1368, 1378
BciVI GTATCC 2 cut(s) 317, 851
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 8 cut(s) 24, 118, 188, 247, 671, 760, 833, 1007
BfmI CTRYAG 3 cut(s) 720, 1026, 1155
BfoI RGCGCY 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 1333
BfuI GTATCC 2 cut(s) 317, 851
BisI GCNGC 3 cut(s) 396, 422, 896
BlsI GCNGC 3 cut(s) 397, 423, 897
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 8 cut(s) 180, 198, 828, 909, 989, 1305, 1368, 1378
Bme18I GGWCC 3 cut(s) 154, 1300, 1322
BmeT110I CYCGRG 1 cut(s) 433
BmgT120I GGNCC 4 cut(s) 154, 486, 1300, 1322
BmiI GGNNCC 2 cut(s) 382, 1302
BmrFI CCNGG 8 cut(s) 180, 198, 828, 909, 989, 1305, 1368, 1378
BmrI ACTGGG 3 cut(s) 1075, 1189, 1543
BmsI GCATC 4 cut(s) 382, 716, 1118, 1516
BmuI ACTGGG 3 cut(s) 1075, 1189, 1543
BpmI CTGGAG 3 cut(s) 333, 884, 948
BpuMI CCSGG 1 cut(s) 180
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 2 cut(s) 188, 671
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 2 cut(s) 1029, 1059
Bsc4I CCNNNNNNNGG 6 cut(s) 232, 311, 555, 827, 1065, 1425
Bse3DI GCAATG 1 cut(s) 1345
BseBI CCWGG 7 cut(s) 198, 828, 909, 989, 1305, 1368, 1378
BseGI GGATG 2 cut(s) 223, 1109
BseLI CCNNNNNNNGG 6 cut(s) 232, 311, 555, 827, 1065, 1425
BseMI GCAATG 1 cut(s) 1345
BseMII CTCAG 2 cut(s) 284, 1245
BseRI GAGGAG 2 cut(s) 951, 1214
BseSI GKGCMC 1 cut(s) 1186
BseXI GCAGC 3 cut(s) 382, 408, 907
Bsh1236I CGCG 2 cut(s) 215, 238
BshFI GGCC 1 cut(s) 487
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 2 cut(s) 179, 226
BslFI GGGAC 5 cut(s) 179, 1054, 1063, 1286, 1441
BslI CCNNNNNNNGG 6 cut(s) 232, 311, 555, 827, 1065, 1425
BsmAI GTCTC 8 cut(s) 24, 118, 188, 247, 671, 760, 833, 1007
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 5 cut(s) 179, 1054, 1063, 1286, 1441
BsmI GAATGC 1 cut(s) 1156
BsnI GGCC 1 cut(s) 487
Bso31I GGTCTC 2 cut(s) 188, 671
BsoBI CYCGRG 1 cut(s) 433
Bsp1286I GDGCHC 3 cut(s) 358, 1186, 1420
Bsp143I GATC 3 cut(s) 565, 1204, 1284
Bsp19I CCATGG 2 cut(s) 1059, 1387
BspACI CCGC 4 cut(s) 213, 215, 238, 1466
BspANI GGCC 1 cut(s) 487
BspCNI CTCAG 2 cut(s) 283, 1246
BspFNI CGCG 2 cut(s) 215, 238
BspLI GGNNCC 2 cut(s) 382, 1302
BspMI ACCTGC 1 cut(s) 1333
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 188, 671
BsrDI GCAATG 1 cut(s) 1345
BssMI GATC 3 cut(s) 565, 1204, 1284
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 3 cut(s) 361, 1059, 1387
Bst2UI CCWGG 7 cut(s) 198, 828, 909, 989, 1305, 1368, 1378
Bst4CI ACNGT 7 cut(s) 18, 59, 753, 870, 1045, 1159, 1268
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 3 cut(s) 270, 515, 1254
BstDSI CCRYGG 3 cut(s) 213, 1059, 1387
BstF5I GGATG 2 cut(s) 223, 1109
BstFNI CGCG 2 cut(s) 215, 238
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 2 cut(s) 238, 383
BstKTI GATC 3 cut(s) 568, 1207, 1287
BstMAI GTCTC 8 cut(s) 24, 118, 188, 247, 671, 760, 833, 1007
BstMBI GATC 3 cut(s) 565, 1204, 1284
BstMWI GCNNNNNNNGC 2 cut(s) 1151, 1181
BstNI CCWGG 7 cut(s) 198, 828, 909, 989, 1305, 1368, 1378
BstNSI RCATGY 1 cut(s) 178
BstSCI CCNGG 8 cut(s) 178, 196, 826, 907, 987, 1303, 1366, 1376
BstSFI CTRYAG 3 cut(s) 720, 1026, 1155
BstSLI GKGCMC 1 cut(s) 1186
BstUI CGCG 2 cut(s) 215, 238
BstV1I GCAGC 3 cut(s) 382, 408, 907
BstXI CCANNNNNNTGG 2 cut(s) 908, 1271
BsuI GTATCC 2 cut(s) 317, 851
BsuRI GGCC 1 cut(s) 487
BtgI CCRYGG 3 cut(s) 213, 1059, 1387
BtgZI GCGATG 1 cut(s) 1422
BtsCI GGATG 2 cut(s) 223, 1109
BtsIMutI CAGTG 4 cut(s) 758, 1164, 1177, 1264
BveI ACCTGC 1 cut(s) 1333
Cac8I GCNNGC 1 cut(s) 234
CaiI CAGNNNCTG 2 cut(s) 256, 1325
CfoI GCGC 2 cut(s) 238, 383
Cfr13I GGNCC 4 cut(s) 154, 486, 1300, 1322
Cfr42I CCGCGG 1 cut(s) 216
Csp6I GTAC 5 cut(s) 274, 985, 1056, 1344, 1482
CviAII CATG 8 cut(s) 147, 175, 340, 545, 848, 1005, 1060, 1388
CviQI GTAC 5 cut(s) 274, 985, 1056, 1344, 1482
DdeI CTNAG 3 cut(s) 270, 515, 1254
DinI GGCGCC 1 cut(s) 382
DpnI GATC 3 cut(s) 567, 1206, 1286
DpnII GATC 3 cut(s) 565, 1204, 1284
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 356
Eco130I CCWWGG 3 cut(s) 361, 1059, 1387
Eco24I GRGCYC 2 cut(s) 358, 1420
Eco31I GGTCTC 2 cut(s) 188, 671
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 3 cut(s) 154, 1300, 1322
Eco53kI GAGCTC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 433
EcoICRI GAGCTC 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRII CCWGG 7 cut(s) 196, 826, 907, 987, 1303, 1366, 1376
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 3 cut(s) 361, 1059, 1387
EcoT38I GRGCYC 2 cut(s) 358, 1420
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
ErhI CCWWGG 3 cut(s) 361, 1059, 1387
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 8 cut(s) 150, 178, 343, 548, 851, 1008, 1063, 1391
FaqI GGGAC 5 cut(s) 179, 1054, 1063, 1286, 1441
FatI CATG 8 cut(s) 146, 174, 339, 544, 847, 1004, 1059, 1387
FauI CCCGC 1 cut(s) 1459
FauNDI CATATG 1 cut(s) 882
Fnu4HI GCNGC 3 cut(s) 396, 422, 896
FokI GGATG 2 cut(s) 230, 1096
FriOI GRGCYC 2 cut(s) 358, 1420
Fsp4HI GCNGC 3 cut(s) 396, 422, 896
GlaI GCGC 2 cut(s) 237, 382
GluI GCNGC 3 cut(s) 396, 422, 896
GsuI CTGGAG 3 cut(s) 333, 884, 948
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 1 cut(s) 487
HapII CCGG 2 cut(s) 179, 226
HhaI GCGC 2 cut(s) 238, 383
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 8 cut(s) 150, 178, 343, 548, 851, 1008, 1063, 1391
Hin6I GCGC 2 cut(s) 236, 381
HinP1I GCGC 2 cut(s) 236, 381
HincII GTYRAC 2 cut(s) 94, 1384
HindII GTYRAC 2 cut(s) 94, 1384
HinfI GANTC 4 cut(s) 797, 904, 920, 1538
HpaI GTTAAC 1 cut(s) 1384
HpaII CCGG 2 cut(s) 179, 226
HphI GGTGA 2 cut(s) 350, 810
Hpy166II GTNNAC 3 cut(s) 94, 818, 1384
Hpy188I TCNGA 7 cut(s) 118, 565, 745, 874, 1132, 1255, 1543
Hpy188III TCNNGA 7 cut(s) 29, 312, 446, 480, 901, 965, 1048
Hpy8I GTNNAC 3 cut(s) 94, 818, 1384
Hpy99I CGWCG 2 cut(s) 74, 192
HpyAV CCTTC 3 cut(s) 474, 477, 1568
HpyCH4III ACNGT 7 cut(s) 18, 59, 753, 870, 1045, 1159, 1268
HpyCH4V TGCA 9 cut(s) 141, 593, 707, 895, 931, 1124, 1145, 1245, 1400
HpyF10VI GCNNNNNNNGC 2 cut(s) 1151, 1181
HpyF3I CTNAG 3 cut(s) 270, 515, 1254
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 8 cut(s) 150, 178, 343, 548, 851, 1008, 1063, 1391
HspAI GCGC 2 cut(s) 236, 381
KasI GGCGCC 1 cut(s) 380
KspAI GTTAAC 1 cut(s) 1384
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 3 cut(s) 565, 1204, 1284
LmnI GCTCC 3 cut(s) 353, 903, 964
Lsp1109I GCAGC 3 cut(s) 382, 408, 907
LweI GCATC 4 cut(s) 382, 716, 1118, 1516
MaeIII GTNAC 4 cut(s) 18, 502, 510, 1370
MalI GATC 3 cut(s) 567, 1206, 1286
MboI GATC 3 cut(s) 565, 1204, 1284
MboII GAAGA 2 cut(s) 355, 508
MhlI GDGCHC 3 cut(s) 358, 1186, 1420
MluCI AATT 6 cut(s) 264, 636, 715, 1098, 1163, 1246
Mly113I GGCGCC 1 cut(s) 381
MlyI GAGTC 2 cut(s) 898, 929
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 9 cut(s) 215, 315, 831, 888, 972, 1031, 1124, 1126, 1235
MseI TTAA 3 cut(s) 714, 1020, 1383
MslI CAYNNNNRTG 3 cut(s) 173, 363, 435
MspA1I CMGCKG 2 cut(s) 215, 1466
MspI CCGG 2 cut(s) 179, 226
MspR9I CCNGG 8 cut(s) 180, 198, 828, 909, 989, 1305, 1368, 1378
Mva1269I GAATGC 1 cut(s) 1156
MvaI CCWGG 7 cut(s) 198, 828, 909, 989, 1305, 1368, 1378
MvnI CGCG 2 cut(s) 215, 238
MwoI GCNNNNNNNGC 2 cut(s) 1151, 1181
NarI GGCGCC 1 cut(s) 381
NciI CCSGG 1 cut(s) 180
NcoI CCATGG 2 cut(s) 1059, 1387
NdeI CATATG 1 cut(s) 882
NdeII GATC 3 cut(s) 565, 1204, 1284
NlaIII CATG 8 cut(s) 150, 178, 343, 548, 851, 1008, 1063, 1391
NlaIV GGNNCC 2 cut(s) 382, 1302
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 1 cut(s) 178
OliI CACNNNNGTG 2 cut(s) 363, 435
PasI CCCWGGG 1 cut(s) 827
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 1156
PfeI GAWTC 2 cut(s) 797, 1538
PflMI CCANNNNNTGG 2 cut(s) 555, 1065
PfoI TCCNGGA 1 cut(s) 907
PkrI GCNGC 3 cut(s) 397, 423, 897
PleI GAGTC 2 cut(s) 898, 928
PluTI GGCGCC 1 cut(s) 384
PpsI GAGTC 2 cut(s) 898, 928
PpuMI RGGWCCY 1 cut(s) 1322
Psp124BI GAGCTC 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 1322
Psp6I CCWGG 7 cut(s) 196, 826, 907, 987, 1303, 1366, 1376
PspGI CCWGG 7 cut(s) 196, 826, 907, 987, 1303, 1366, 1376
PspN4I GGNNCC 2 cut(s) 382, 1302
PspPI GGNCC 4 cut(s) 154, 486, 1300, 1322
PspPPI RGGWCCY 1 cut(s) 1322
PsrI GAACNNNNNNTAC 2 cut(s) 1395, 1427
PstNI CAGNNNCTG 2 cut(s) 256, 1325
RsaI GTAC 5 cut(s) 275, 986, 1057, 1345, 1483
RsaNI GTAC 5 cut(s) 274, 985, 1056, 1344, 1482
RseI CAYNNNNRTG 3 cut(s) 173, 363, 435
SacI GAGCTC 1 cut(s) 358
SacII CCGCGG 1 cut(s) 216
SaqAI TTAA 3 cut(s) 714, 1020, 1383
SatI GCNGC 3 cut(s) 396, 422, 896
Sau3AI GATC 3 cut(s) 565, 1204, 1284
Sau96I GGNCC 4 cut(s) 154, 486, 1300, 1322
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 898, 929
ScrFI CCNGG 8 cut(s) 180, 198, 828, 909, 989, 1305, 1368, 1378
SduI GDGCHC 3 cut(s) 358, 1186, 1420
SfaNI GCATC 4 cut(s) 382, 716, 1118, 1516
SfcI CTRYAG 3 cut(s) 720, 1026, 1155
SfoI GGCGCC 1 cut(s) 382
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 3 cut(s) 154, 1300, 1322
SmiMI CAYNNNNRTG 3 cut(s) 173, 363, 435
Sse9I AATT 6 cut(s) 264, 636, 715, 1098, 1163, 1246
SsiI CCGC 4 cut(s) 213, 215, 238, 1466
SspDI GGCGCC 1 cut(s) 380
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 8 cut(s) 178, 196, 826, 907, 987, 1303, 1366, 1376
StyI CCWWGG 3 cut(s) 361, 1059, 1387
TaaI ACNGT 7 cut(s) 18, 59, 753, 870, 1045, 1159, 1268
TaqI TCGA 3 cut(s) 28, 1316, 1414
TaqII GACCGA 1 cut(s) 776
TasI AATT 6 cut(s) 264, 636, 715, 1098, 1163, 1246
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 2 cut(s) 797, 1538
Tru1I TTAA 3 cut(s) 714, 1020, 1383
Tru9I TTAA 3 cut(s) 714, 1020, 1383
TscAI CASTG 4 cut(s) 758, 1164, 1184, 1271
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 3 cut(s) 395, 421, 895
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 5 cut(s) 59, 356, 1011, 1103, 1239
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 4 cut(s) 758, 1164, 1184, 1271
Van91I CCANNNNNTGG 2 cut(s) 555, 1065
VpaK11BI GGWCC 3 cut(s) 154, 1300, 1322
XapI RAATTY 1 cut(s) 636
XceI RCATGY 1 cut(s) 178
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.