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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G13590 AT5G13590 AT5G13590 AT5G13590 AT5G13590 AT5G13590 |
| fragaria_vesca | FvH4_7g20760 |
| malus_domestica | MD01G1117200.v1.1 MD07G1184600.v1.1 |
| prunus_persica | Prupe.2G224500_v2.0.a1 |
| pyrus_communis | pycom01g14480 pycom07g17670 |
| rosa_chinensis | RchiOBHm_Chr1g0365001 |
| rosa_laevigata | RLG00000027451 |
| rosa_multiflora | Rmu_sc0000418.1_g000009 Rmu_ssc0000112.1_g000020 |
| rosa_roxburghii | Rroxscaffold_1G00022860 Rroxscaffold_2G00110440 Rroxscaffold_4G00291580 Rroxscaffold_4G00292010 |
| rosa_rugosa | Rorug01G0318800 Rorug02G0315200 |
| rosa_samantha | Rh1AG327800 Rh1BG289100 Rh1CG305800 Rh1DG320600 |
| rosa_wichuraiana | Rw1G029060 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 4 cut(s) | 2369, 2655, 2733, 3540 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 862, 1261 |
| AbsI | CCTCGAGG | 1 cut(s) | 2496 |
| Acc36I | ACCTGC | 1 cut(s) | 2470 |
| Acc65I | GGTACC | 1 cut(s) | 2544 |
| AccB1I | GGYRCC | 1 cut(s) | 2544 |
| AccBSI | CCGCTC | 2 cut(s) | 425, 2542 |
| AccI | GTMKAC | 4 cut(s) | 98, 1265, 1397, 2809 |
| AccIII | TCCGGA | 1 cut(s) | 1312 |
| AciI | CCGC | 8 cut(s) | 425, 2356, 2542, 2642, 3230, 3269, 3401, 3422 |
| AclWI | GGATC | 7 cut(s) | 755, 1202, 1209, 2969, 3141, 3213, 3380 |
| AcoI | YGGCCR | 1 cut(s) | 2761 |
| AcsI | RAATTY | 9 cut(s) | 135, 358, 580, 1199, 2454, 2566, 2575, 2875, 3730 |
| AcuI | CTGAAG | 5 cut(s) | 48, 261, 393, 2727, 2895 |
| AfaI | GTAC | 7 cut(s) | 66, 469, 1696, 2546, 2669, 2992, 3523 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 91, 553, 1936, 2535, 2837, 3229 |
| AflIII | ACRYGT | 1 cut(s) | 1297 |
| AhlI | ACTAGT | 1 cut(s) | 125 |
| AjiI | CACGTC | 1 cut(s) | 2585 |
| AjnI | CCWGG | 5 cut(s) | 157, 1521, 1815, 3169, 3682 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 3100, 3132 |
| Alw21I | GWGCWC | 4 cut(s) | 655, 1827, 2090, 2239 |
| AlwI | GGATC | 7 cut(s) | 755, 1202, 1209, 2969, 3141, 3213, 3380 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 737 |
| Ama87I | CYCGRG | 2 cut(s) | 2496, 3390 |
| Aor13HI | TCCGGA | 1 cut(s) | 1312 |
| ApeKI | GCWGC | 5 cut(s) | 998, 1349, 1405, 1477, 2639 |
| ApoI | RAATTY | 9 cut(s) | 135, 358, 580, 1199, 2454, 2566, 2575, 2875, 3730 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1378, 2274, 3112 |
| Asp718I | GGTACC | 1 cut(s) | 2544 |
| AspA2I | CCTAGG | 2 cut(s) | 1010, 3587 |
| AsuHPI | GGTGA | 8 cut(s) | 9, 536, 2330, 2519, 2912, 3512, 3566, 3626 |
| AvaI | CYCGRG | 2 cut(s) | 2496, 3390 |
| AvrII | CCTAGG | 2 cut(s) | 1010, 3587 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1797, 1830 |
| BanI | GGYRCC | 1 cut(s) | 2544 |
| BanII | GRGCYC | 2 cut(s) | 1827, 2090 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2983, 3015 |
| BauI | CACGAG | 1 cut(s) | 1623 |
| Bbv12I | GWGCWC | 4 cut(s) | 655, 1827, 2090, 2239 |
| BbvCI | CCTCAGC | 1 cut(s) | 462 |
| BbvI | GCAGC | 5 cut(s) | 985, 1336, 1417, 1464, 2651 |
| BceAI | ACGGC | 1 cut(s) | 2748 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 3428, 3462 |
| BciT130I | CCWGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| BciVI | GTATCC | 4 cut(s) | 1798, 2236, 2329, 3286 |
| BclI | TGATCA | 3 cut(s) | 1606, 1984, 2140 |
| BcuI | ACTAGT | 1 cut(s) | 125 |
| BfmI | CTRYAG | 6 cut(s) | 1350, 1725, 2205, 2223, 2530, 2970 |
| BfuAI | ACCTGC | 1 cut(s) | 2470 |
| BfuI | GTATCC | 4 cut(s) | 1798, 2236, 2329, 3286 |
| BglII | AGATCT | 1 cut(s) | 2008 |
| BisI | GCNGC | 6 cut(s) | 999, 1350, 1406, 1478, 2640, 3231 |
| BlnI | CCTAGG | 2 cut(s) | 1010, 3587 |
| BlsI | GCNGC | 6 cut(s) | 1000, 1351, 1407, 1479, 2641, 3232 |
| Bme1390I | CCNGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| BmeT110I | CYCGRG | 2 cut(s) | 2496, 3390 |
| BmgBI | CACGTC | 1 cut(s) | 2585 |
| BmrFI | CCNGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| BmrI | ACTGGG | 3 cut(s) | 688, 1095, 1410 |
| BmsI | GCATC | 8 cut(s) | 133, 438, 691, 715, 2167, 2882, 3217, 3242 |
| BmuI | ACTGGG | 3 cut(s) | 688, 1095, 1410 |
| BoxI | GACNNNNGTC | 1 cut(s) | 3309 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 2174, 2206, 2812, 2844 |
| BpmI | CTGGAG | 3 cut(s) | 714, 3192, 3264 |
| Bpu10I | CCTNAGC | 2 cut(s) | 462, 2238 |
| BpuEI | CTTGAG | 2 cut(s) | 1259, 2800 |
| BsaBI | GATNNNNATC | 4 cut(s) | 1716, 2007, 2410, 3217 |
| BsaI | GGTCTC | 4 cut(s) | 358, 2400, 2832, 3297 |
| BsaWI | WCCGGW | 2 cut(s) | 1312, 3597 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 91, 553, 1936, 2535, 2837, 3229 |
| Bse1I | ACTGG | 8 cut(s) | 683, 731, 1101, 1293, 1405, 1641, 2340, 3247 |
| Bse3DI | GCAATG | 1 cut(s) | 966 |
| Bse8I | GATNNNNATC | 4 cut(s) | 1716, 2007, 2410, 3217 |
| BseAI | TCCGGA | 1 cut(s) | 1312 |
| BseBI | CCWGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| BseJI | GATNNNNATC | 4 cut(s) | 1716, 2007, 2410, 3217 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 91, 553, 1936, 2535, 2837, 3229 |
| BseMI | GCAATG | 1 cut(s) | 966 |
| BseMII | CTCAG | 9 cut(s) | 104, 453, 668, 825, 1451, 2082, 2238, 2252, 3612 |
| BseNI | ACTGG | 8 cut(s) | 683, 731, 1101, 1293, 1405, 1641, 2340, 3247 |
| BseRI | GAGGAG | 3 cut(s) | 1168, 2167, 3502 |
| BseXI | GCAGC | 5 cut(s) | 985, 1336, 1417, 1464, 2651 |
| BsgI | GTGCAG | 1 cut(s) | 309 |
| BshNI | GGYRCC | 1 cut(s) | 2544 |
| BsiHKAI | GWGCWC | 4 cut(s) | 655, 1827, 2090, 2239 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2496, 3390 |
| BsiSI | CCGG | 3 cut(s) | 1313, 2362, 3598 |
| BslFI | GGGAC | 5 cut(s) | 780, 1125, 1911, 2853, 3525 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 91, 553, 1936, 2535, 2837, 3229 |
| BsmBI | CGTCTC | 3 cut(s) | 1247, 2815, 3499 |
| BsmFI | GGGAC | 5 cut(s) | 780, 1125, 1911, 2853, 3525 |
| BsmI | GAATGC | 2 cut(s) | 3327, 3439 |
| Bso31I | GGTCTC | 4 cut(s) | 358, 2400, 2832, 3297 |
| BsoBI | CYCGRG | 2 cut(s) | 2496, 3390 |
| Bsp1286I | GDGCHC | 4 cut(s) | 655, 1827, 2090, 2239 |
| Bsp13I | TCCGGA | 1 cut(s) | 1312 |
| Bsp19I | CCATGG | 4 cut(s) | 646, 3125, 3307, 3752 |
| BspACI | CCGC | 8 cut(s) | 425, 2356, 2542, 2642, 3230, 3269, 3401, 3422 |
| BspCNI | CTCAG | 9 cut(s) | 103, 454, 667, 824, 1450, 2083, 2239, 2251, 3613 |
| BspEI | TCCGGA | 1 cut(s) | 1312 |
| BspHI | TCATGA | 1 cut(s) | 1987 |
| BspMAI | CTGCAG | 2 cut(s) | 1354, 2227 |
| BspMI | ACCTGC | 1 cut(s) | 2470 |
| BspPI | GGATC | 7 cut(s) | 755, 1202, 1209, 2969, 3141, 3213, 3380 |
| BspT107I | GGYRCC | 1 cut(s) | 2544 |
| BspTNI | GGTCTC | 4 cut(s) | 358, 2400, 2832, 3297 |
| BsrBI | CCGCTC | 2 cut(s) | 425, 2542 |
| BsrDI | GCAATG | 1 cut(s) | 966 |
| BsrI | ACTGG | 8 cut(s) | 683, 731, 1101, 1293, 1405, 1641, 2340, 3247 |
| BssNAI | GTATAC | 1 cut(s) | 2810 |
| BssSI | CACGAG | 1 cut(s) | 1623 |
| BssT1I | CCWWGG | 8 cut(s) | 211, 646, 1010, 2215, 3125, 3307, 3587, 3752 |
| Bst1107I | GTATAC | 1 cut(s) | 2810 |
| Bst2BI | CACGAG | 1 cut(s) | 1623 |
| Bst2UI | CCWGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| Bst6I | CTCTTC | 5 cut(s) | 169, 438, 909, 2443, 3785 |
| BstC8I | GCNNGC | 4 cut(s) | 663, 881, 2054, 2354 |
| BstDSI | CCRYGG | 6 cut(s) | 646, 2608, 2764, 3125, 3307, 3752 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2533 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 152, 671, 889, 2094, 2358 |
| BstNI | CCWGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| BstNSI | RCATGY | 4 cut(s) | 669, 773, 1301, 1552 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 3309 |
| BstSCI | CCNGG | 5 cut(s) | 157, 1521, 1815, 3169, 3682 |
| BstSFI | CTRYAG | 6 cut(s) | 1350, 1725, 2205, 2223, 2530, 2970 |
| BstV1I | GCAGC | 5 cut(s) | 985, 1336, 1417, 1464, 2651 |
| BstX2I | RGATCY | 6 cut(s) | 1214, 2008, 2974, 3146, 3218, 3385 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1108 |
| BstYI | RGATCY | 6 cut(s) | 1214, 2008, 2974, 3146, 3218, 3385 |
| BstZ17I | GTATAC | 1 cut(s) | 2810 |
| BsuI | GTATCC | 4 cut(s) | 1798, 2236, 2329, 3286 |
| BtgI | CCRYGG | 6 cut(s) | 646, 2608, 2764, 3125, 3307, 3752 |
| BtgZI | GCGATG | 1 cut(s) | 3756 |
| BtrI | CACGTC | 1 cut(s) | 2585 |
| BtsI | GCAGTG | 1 cut(s) | 932 |
| BtsIMutI | CAGTG | 8 cut(s) | 613, 757, 932, 1158, 1648, 2115, 2577, 3550 |
| BveI | ACCTGC | 1 cut(s) | 2470 |
| Cac8I | GCNNGC | 4 cut(s) | 663, 881, 2054, 2354 |
| CaiI | CAGNNNCTG | 1 cut(s) | 737 |
| CciI | TCATGA | 1 cut(s) | 1987 |
| CseI | GACGC | 1 cut(s) | 2717 |
| Csp6I | GTAC | 7 cut(s) | 65, 468, 1695, 2545, 2668, 2991, 3522 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1827, 1862 |
| CviQI | GTAC | 7 cut(s) | 65, 468, 1695, 2545, 2668, 2991, 3522 |
| DraI | TTTAAA | 1 cut(s) | 2388 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 862, 1261 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 862, 1261 |
| EaeI | YGGCCR | 1 cut(s) | 2761 |
| Eam1104I | CTCTTC | 5 cut(s) | 169, 438, 909, 2443, 3785 |
| EarI | CTCTTC | 5 cut(s) | 169, 438, 909, 2443, 3785 |
| EciI | GGCGGA | 2 cut(s) | 3258, 3416 |
| Ecl136II | GAGCTC | 2 cut(s) | 1825, 2088 |
| Eco130I | CCWWGG | 8 cut(s) | 211, 646, 1010, 2215, 3125, 3307, 3587, 3752 |
| Eco147I | AGGCCT | 2 cut(s) | 3532, 3592 |
| Eco24I | GRGCYC | 2 cut(s) | 1827, 2090 |
| Eco31I | GGTCTC | 4 cut(s) | 358, 2400, 2832, 3297 |
| Eco32I | GATATC | 2 cut(s) | 1359, 3778 |
| Eco53kI | GAGCTC | 2 cut(s) | 1825, 2088 |
| Eco57I | CTGAAG | 5 cut(s) | 48, 261, 393, 2727, 2895 |
| Eco88I | CYCGRG | 2 cut(s) | 2496, 3390 |
| EcoICRI | GAGCTC | 2 cut(s) | 1825, 2088 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2533 |
| EcoO109I | RGGNCCY | 5 cut(s) | 1898, 2840, 3045, 3431, 3512 |
| EcoRI | GAATTC | 1 cut(s) | 2566 |
| EcoRII | CCWGG | 5 cut(s) | 157, 1521, 1815, 3169, 3682 |
| EcoRV | GATATC | 2 cut(s) | 1359, 3778 |
| EcoT14I | CCWWGG | 8 cut(s) | 211, 646, 1010, 2215, 3125, 3307, 3587, 3752 |
| EcoT22I | ATGCAT | 4 cut(s) | 411, 706, 2897, 3441 |
| EcoT38I | GRGCYC | 2 cut(s) | 1827, 2090 |
| ErhI | CCWWGG | 8 cut(s) | 211, 646, 1010, 2215, 3125, 3307, 3587, 3752 |
| Esp3I | CGTCTC | 3 cut(s) | 1247, 2815, 3499 |
| FaqI | GGGAC | 5 cut(s) | 780, 1125, 1911, 2853, 3525 |
| FauI | CCCGC | 1 cut(s) | 3415 |
| FauNDI | CATATG | 1 cut(s) | 3004 |
| FbaI | TGATCA | 3 cut(s) | 1606, 1984, 2140 |
| FblI | GTMKAC | 4 cut(s) | 98, 1265, 1397, 2809 |
| Fnu4HI | GCNGC | 6 cut(s) | 999, 1350, 1406, 1478, 2640, 3231 |
| FriOI | GRGCYC | 2 cut(s) | 1827, 2090 |
| Fsp4HI | GCNGC | 6 cut(s) | 999, 1350, 1406, 1478, 2640, 3231 |
| GluI | GCNGC | 6 cut(s) | 999, 1350, 1406, 1478, 2640, 3231 |
| GsuI | CTGGAG | 3 cut(s) | 714, 3192, 3264 |
| HapII | CCGG | 3 cut(s) | 1313, 2362, 3598 |
| HgaI | GACGC | 1 cut(s) | 2717 |
| HincII | GTYRAC | 5 cut(s) | 99, 1398, 1428, 2521, 3571 |
| HindII | GTYRAC | 5 cut(s) | 99, 1398, 1428, 2521, 3571 |
| HindIII | AAGCTT | 3 cut(s) | 1488, 2777, 2942 |
| HpaI | GTTAAC | 1 cut(s) | 3571 |
| HpaII | CCGG | 3 cut(s) | 1313, 2362, 3598 |
| HphI | GGTGA | 8 cut(s) | 9, 536, 2330, 2519, 2912, 3512, 3566, 3626 |
| Hpy166II | GTNNAC | 9 cut(s) | 99, 1174, 1266, 1398, 1428, 1543, 2521, 2810, 3571 |
| Hpy8I | GTNNAC | 9 cut(s) | 99, 1174, 1266, 1398, 1428, 1543, 2521, 2810, 3571 |
| HpyCH4IV | ACGT | 3 cut(s) | 496, 2513, 2584 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 152, 671, 889, 2094, 2358 |
| HpySE526I | ACGT | 3 cut(s) | 496, 2513, 2584 |
| Kpn2I | TCCGGA | 1 cut(s) | 1312 |
| KpnI | GGTACC | 1 cut(s) | 2548 |
| Ksp22I | TGATCA | 3 cut(s) | 1606, 1984, 2140 |
| KspAI | GTTAAC | 1 cut(s) | 3571 |
| LmnI | GCTCC | 9 cut(s) | 160, 382, 733, 1166, 2194, 2358, 2539, 2644, 3738 |
| Lsp1109I | GCAGC | 5 cut(s) | 985, 1336, 1417, 1464, 2651 |
| LweI | GCATC | 8 cut(s) | 133, 438, 691, 715, 2167, 2882, 3217, 3242 |
| MaeII | ACGT | 3 cut(s) | 496, 2513, 2584 |
| MaeIII | GTNAC | 7 cut(s) | 542, 836, 1432, 2119, 2200, 2467, 2630 |
| MbiI | CCGCTC | 2 cut(s) | 425, 2542 |
| MfeI | CAATTG | 1 cut(s) | 783 |
| MflI | RGATCY | 6 cut(s) | 1214, 2008, 2974, 3146, 3218, 3385 |
| MhlI | GDGCHC | 4 cut(s) | 655, 1827, 2090, 2239 |
| MlyI | GAGTC | 9 cut(s) | 243, 550, 844, 1095, 1403, 1429, 1457, 1844, 3346 |
| MmeI | TCCRAC | 3 cut(s) | 960, 1144, 1309 |
| Mph1103I | ATGCAT | 4 cut(s) | 411, 706, 2897, 3441 |
| MroI | TCCGGA | 1 cut(s) | 1312 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1378, 2274, 3112 |
| MslI | CAYNNNNRTG | 2 cut(s) | 114, 2688 |
| MspA1I | CMGCKG | 2 cut(s) | 2356, 2642 |
| MspI | CCGG | 3 cut(s) | 1313, 2362, 3598 |
| MspR9I | CCNGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| MunI | CAATTG | 1 cut(s) | 783 |
| Mva1269I | GAATGC | 2 cut(s) | 3327, 3439 |
| MvaI | CCWGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 152, 671, 889, 2094, 2358 |
| NcoI | CCATGG | 4 cut(s) | 646, 3125, 3307, 3752 |
| NdeI | CATATG | 1 cut(s) | 3004 |
| NmuCI | GTSAC | 5 cut(s) | 542, 836, 1432, 2119, 2200 |
| NsiI | ATGCAT | 4 cut(s) | 411, 706, 2897, 3441 |
| NspI | RCATGY | 4 cut(s) | 669, 773, 1301, 1552 |
| PaeR7I | CTCGAG | 2 cut(s) | 2496, 3390 |
| PagI | TCATGA | 1 cut(s) | 1987 |
| PasI | CCCWGGG | 1 cut(s) | 1522 |
| PceI | AGGCCT | 2 cut(s) | 3532, 3592 |
| PciI | ACATGT | 1 cut(s) | 1297 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2715 |
| PctI | GAATGC | 2 cut(s) | 3327, 3439 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1378, 2274, 3112 |
| PflFI | GACNNNGTC | 1 cut(s) | 1393 |
| PfoI | TCCNGGA | 1 cut(s) | 1815 |
| PkrI | GCNGC | 6 cut(s) | 1000, 1351, 1407, 1479, 2641, 3232 |
| PleI | GAGTC | 9 cut(s) | 242, 549, 843, 1094, 1402, 1429, 1457, 1843, 3345 |
| PpsI | GAGTC | 9 cut(s) | 242, 549, 843, 1094, 1402, 1429, 1457, 1843, 3345 |
| PpuMI | RGGWCCY | 4 cut(s) | 1898, 2840, 3045, 3512 |
| PscI | ACATGT | 1 cut(s) | 1297 |
| PshAI | GACNNNNGTC | 1 cut(s) | 3309 |
| PsiI | TTATAA | 4 cut(s) | 2369, 2655, 2733, 3540 |
| Psp124BI | GAGCTC | 2 cut(s) | 1827, 2090 |
| Psp5II | RGGWCCY | 4 cut(s) | 1898, 2840, 3045, 3512 |
| Psp6I | CCWGG | 5 cut(s) | 157, 1521, 1815, 3169, 3682 |
| PspGI | CCWGG | 5 cut(s) | 157, 1521, 1815, 3169, 3682 |
| PspPPI | RGGWCCY | 4 cut(s) | 1898, 2840, 3045, 3512 |
| PspXI | VCTCGAGB | 1 cut(s) | 2496 |
| PstI | CTGCAG | 2 cut(s) | 1354, 2227 |
| PstNI | CAGNNNCTG | 1 cut(s) | 737 |
| PsuI | RGATCY | 6 cut(s) | 1214, 2008, 2974, 3146, 3218, 3385 |
| PsyI | GACNNNGTC | 1 cut(s) | 1393 |
| RsaI | GTAC | 7 cut(s) | 66, 469, 1696, 2546, 2669, 2992, 3523 |
| RsaNI | GTAC | 7 cut(s) | 65, 468, 1695, 2545, 2668, 2991, 3522 |
| RseI | CAYNNNNRTG | 2 cut(s) | 114, 2688 |
| SacI | GAGCTC | 2 cut(s) | 1827, 2090 |
| SalI | GTCGAC | 2 cut(s) | 97, 1396 |
| SatI | GCNGC | 6 cut(s) | 999, 1350, 1406, 1478, 2640, 3231 |
| SchI | GAGTC | 9 cut(s) | 243, 550, 844, 1095, 1403, 1429, 1457, 1844, 3346 |
| ScrFI | CCNGG | 5 cut(s) | 159, 1523, 1817, 3171, 3684 |
| SduI | GDGCHC | 4 cut(s) | 655, 1827, 2090, 2239 |
| SfaNI | GCATC | 8 cut(s) | 133, 438, 691, 715, 2167, 2882, 3217, 3242 |
| SfcI | CTRYAG | 6 cut(s) | 1350, 1725, 2205, 2223, 2530, 2970 |
| Sfr274I | CTCGAG | 2 cut(s) | 2496, 3390 |
| SlaI | CTCGAG | 2 cut(s) | 2496, 3390 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 114, 2688 |
| SmlI | CTYRAG | 4 cut(s) | 1238, 2496, 2815, 3390 |
| SmoI | CTYRAG | 4 cut(s) | 1238, 2496, 2815, 3390 |
| SpeI | ACTAGT | 1 cut(s) | 125 |
| SseBI | AGGCCT | 2 cut(s) | 3532, 3592 |
| SsiI | CCGC | 8 cut(s) | 425, 2356, 2542, 2642, 3230, 3269, 3401, 3422 |
| SspI | AATATT | 2 cut(s) | 1123, 2017 |
| SstI | GAGCTC | 2 cut(s) | 1827, 2090 |
| StuI | AGGCCT | 2 cut(s) | 3532, 3592 |
| StyD4I | CCNGG | 5 cut(s) | 157, 1521, 1815, 3169, 3682 |
| StyI | CCWWGG | 8 cut(s) | 211, 646, 1010, 2215, 3125, 3307, 3587, 3752 |
| TaiI | ACGT | 3 cut(s) | 499, 2516, 2587 |
| TaqI | TCGA | 8 cut(s) | 98, 1397, 2380, 2497, 2718, 3076, 3187, 3391 |
| TatI | WGTACW | 1 cut(s) | 64 |
| TauI | GCSGC | 1 cut(s) | 3233 |
| TscAI | CASTG | 8 cut(s) | 613, 757, 939, 1165, 1648, 2122, 2577, 3550 |
| TseFI | GTSAC | 5 cut(s) | 542, 836, 1432, 2119, 2200 |
| TseI | GCWGC | 5 cut(s) | 998, 1349, 1405, 1477, 2639 |
| Tsp45I | GTSAC | 5 cut(s) | 542, 836, 1432, 2119, 2200 |
| TspGWI | ACGGA | 1 cut(s) | 3509 |
| TspRI | CASTG | 8 cut(s) | 613, 757, 939, 1165, 1648, 2122, 2577, 3550 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1393 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2533 |
| XapI | RAATTY | 9 cut(s) | 135, 358, 580, 1199, 2454, 2566, 2575, 2875, 3730 |
| XceI | RCATGY | 4 cut(s) | 669, 773, 1301, 1552 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 702 |
| XhoI | CTCGAG | 2 cut(s) | 2496, 3390 |
| XmaJI | CCTAGG | 2 cut(s) | 1010, 3587 |
| XmiI | GTMKAC | 4 cut(s) | 98, 1265, 1397, 2809 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1378, 2274, 3112 |
| Zsp2I | ATGCAT | 4 cut(s) | 411, 706, 2897, 3441 |