Rh1DG146400

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002387
Physical Location & Seq
Reverse (-)
30723301 .. 30738355
15055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG146400.1

Sequence Viewer

Length: 1776 bp
ATGCATACTGTGCTTTCGGTTTCATCTTCTGCAGTGGAATGGATCAAATCAGCTCGCCTCTCCTCCAGCTTAAAATCTTCTGTCAGAACATCACCTTCTAAGCCATACATGAAGCAAAAACGTCGTCGTTGTAGAGAGAAAGAGAGTAGAAGATCGAAGAAGGATGATGGTGGAGGATCTTGGTGTGGAAGCCAAGGAAGCAGCGCGCGGTTCGCGAGGTTGCGAAGCTGTTGCCTCTGCCGGACCTCCTTCAGTCTATCGCTTCCATTAAGGCCGATTACATTGCTCTGCCAACAGGCAAATGATGCACAACTTAGTACAATGGTTGCCGAGCAGGTAGAACAAGCGCAAGCTGGGCTGGAATCCCTGTCCTTATCTCAAAAATCAATAAACCAACTCCATGAAAACTTTGTTTCTATTGAAAAGCTATATCAAGAATGCCAAACTTTGATTGAAAACCATGATAAGATAAAGCTTCTCAGCAATGCGAGGAATAACTTGAACACAACCTTAAAGGATGTCGAGGGTATGATGTCAATCTCTGTTGAAGCCTCTGAGGCTCGGGATTCTTTTGAGTTAACAGCTCTTGATGGAAAGCCGAGGTTTGCATTAGCAGCTGCAGGATCCCACAAGGAAGAGGTTGGCAGGCTAAGGGAATATTTTGAAGATGTTGATAGGACCTGGGAGACATTTGAGAAGACGCTATGGTGCCACATTTCCAACTTCTATAATCTTTCCAAAGAAAGCCCGCAAACTCTGGCGCGCGCTTTAAGAGTTGTTCAACTGCAAGACATCCTTGACCAACAACTTGCTGAAGAAGCAGCTGAGGCTGAAGGAGGTGGTGCCATGGCATCAATTGCCAATCCTCGTAGAACTGCCAAAAAAACTACCACAGCAATAGCTTCTTCGAGAAATATGACACAGCAGAAACTGAATATACAAGGCAAAGGCTACAAGGACAAATGCTATGATCAAATTAGGAAGACTGTTGAAGGAAGATTCAATAGGCTACTGACAGAGCTTTGTTTCAAGGATTTTAAAGCAGCACTAGAGGAAGCTCGAACAATTGGAGAAGAGCTTGGAGATATATACGACCATGCAGCCCCTTGTTTTCCCCCCAGATATGAAATATTCCAACTCATGGTCAATCTGTATACCGAGAGATTTGTTCGAATGCTTCGACTCCTCAGTGACAGGGCAAATGAAATGACGAACATAGAGATCTTAAAAGTAACTGGTTGGGTAGTTGAATACCAAGAAAACCTGATTGGGCTTGGTGTCGATGAATCTCTTGCTCAAGTATGTTCTGAGAGTGGTTCCATGGATCCCCTAATGAATTCCTATGTGGAGAGAATGCAAGCTACGACAAGGAAATGGTACTTGAATATCTCGGAGGCTGAAACACCGAAGAAAACAGAAGATGGGAAGCTATATACACCAGCTGCTGTTGATTTATTCAGGATCCTTGGAGAACAAGCGCAAATTGTTCGCGACAATAGCACGGATCTCATGCTTTACAGAATTGCTTTAGCAATTATCCAGCGGAGACTTGAGGAACCTGCTTCAGAAGTTGGTTTGGAACCCTTATGTGCAATGATTAACAACAATCTGCGCTGCTATGATCTCACGATGGAGCTAAGTAATAGCACTTTGGAAGCTCTTCCACAAAATTATGCTGAGCAGGAAGCTGTGCATCAAACTATCAGTGTTATTTTTGAAGATCCCGGAGTGCAGTATTTACTTGTGAAGCTTTATCAGAAGGGTAAGGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec6

Protein Analysis

591

Amino Acids

66.79

Weight (kDa)

6.14

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec6 PF06046 221 - 562 3.7e-59 Exocyst complex component Sec6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 325, 1567
AccB1I GGYRCC 2 cut(s) 708, 842
AccB7I CCANNNNNTGG 1 cut(s) 1141
AccI GTMKAC 1 cut(s) 1154
AccII CGCG 6 cut(s) 206, 208, 215, 763, 765, 1491
AciI CCGC 3 cut(s) 208, 749, 1543
AcsI RAATTY 1 cut(s) 1336
AcuI CTGAAG 4 cut(s) 235, 834, 852, 1548
AfaI GTAC 2 cut(s) 319, 1379
AfiI CCNNNNNNNGG 1 cut(s) 1141
AjnI CCWGG 1 cut(s) 680
AjuI GAANNNNNNNTTGG 2 cut(s) 1251, 1283
Alw26I GTCTC 2 cut(s) 680, 1540
AlwNI CAGNNNCTG 2 cut(s) 931, 1445
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 8 cut(s) 201, 614, 617, 821, 1043, 1100, 1442, 1614
ApoI RAATTY 1 cut(s) 1336
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
Asp700I GAANNNNTTC 1 cut(s) 1659
AspLEI GCGC 8 cut(s) 206, 208, 349, 763, 765, 767, 1480, 1614
AspS9I GGNCC 2 cut(s) 243, 678
AsuC2I CCSGG 1 cut(s) 1725
AsuHPI GGTGA 1 cut(s) 84
AsuII TTCGAA 1 cut(s) 1171
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 2 cut(s) 243, 678
BamHI GGATCC 3 cut(s) 623, 1324, 1461
BanI GGYRCC 2 cut(s) 708, 842
BbsI GAAGAC 2 cut(s) 704, 989
BbvCI CCTCAGC 1 cut(s) 825
BbvI GCAGC 8 cut(s) 213, 604, 626, 833, 1055, 1112, 1429, 1601
BccI CCATC 4 cut(s) 161, 584, 1415, 1624
BcgI CGANNNNNNTGC 8 cut(s) 104, 138, 213, 247, 265, 299, 1469, 1503
BciT130I CCWGG 1 cut(s) 682
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 1 cut(s) 1725
BcoDI GTCTC 2 cut(s) 680, 1540
BfaI CTAG 1 cut(s) 1049
BfmI CTRYAG 2 cut(s) 30, 618
BfuAI ACCTGC 2 cut(s) 325, 1567
BglI GCCNNNNNGGC 1 cut(s) 557
BglII AGATCT 1 cut(s) 1221
BisI GCNGC 8 cut(s) 202, 615, 618, 822, 1044, 1101, 1443, 1615
BlpI GCTNAGC 1 cut(s) 1677
BlsI GCNGC 8 cut(s) 203, 616, 619, 823, 1045, 1102, 1444, 1616
Bme1390I CCNGG 2 cut(s) 682, 1725
Bme18I GGWCC 2 cut(s) 243, 678
BmeT110I CYCGRG 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 243, 678
BmiI GGNNCC 8 cut(s) 625, 710, 844, 1318, 1326, 1463, 1557, 1581
BmrFI CCNGG 2 cut(s) 682, 1725
BmsI GCATC 3 cut(s) 295, 860, 1702
BpiI GAAGAC 2 cut(s) 704, 989
BpmI CTGGAG 1 cut(s) 49
Bpu10I CCTNAGC 2 cut(s) 650, 825
Bpu1102I GCTNAGC 1 cut(s) 1677
Bpu14I TTCGAA 1 cut(s) 1171
BpuEI CTTGAG 2 cut(s) 1281, 1571
BpuMI CCSGG 1 cut(s) 1725
BsaBI GATNNNNATC 1 cut(s) 536
BsaJI CCNNGG 6 cut(s) 193, 599, 681, 846, 1320, 1465
Bsc4I CCNNNNNNNGG 1 cut(s) 1141
Bse1I ACTGG 1 cut(s) 1240
Bse3DI GCAATG 3 cut(s) 281, 490, 1599
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 1 cut(s) 682
BseDI CCNNGG 6 cut(s) 193, 599, 681, 846, 1320, 1465
BseGI GGATG 3 cut(s) 169, 523, 792
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 1 cut(s) 1141
BseMI GCAATG 3 cut(s) 281, 490, 1599
BseMII CTCAG 6 cut(s) 493, 546, 816, 1201, 1299, 1668
BseNI ACTGG 1 cut(s) 1240
BsePI GCGCGC 3 cut(s) 204, 761, 763
BseRI GAGGAG 2 cut(s) 52, 1175
BseXI GCAGC 8 cut(s) 213, 604, 626, 833, 1055, 1112, 1429, 1601
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 1751
Bsh1236I CGCG 6 cut(s) 206, 208, 215, 763, 765, 1491
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 2 cut(s) 708, 842
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 2 cut(s) 241, 1725
BslI CCNNNNNNNGG 1 cut(s) 1141
BsmAI GTCTC 2 cut(s) 680, 1540
BsmI GAATGC 3 cut(s) 443, 1179, 1359
BsnI GGCC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 561
Bsp119I TTCGAA 1 cut(s) 1171
Bsp1720I GCTNAGC 1 cut(s) 1677
Bsp19I CCATGG 2 cut(s) 846, 1320
Bsp68I TCGCGA 2 cut(s) 215, 1491
BspACI CCGC 3 cut(s) 208, 749, 1543
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 6 cut(s) 492, 547, 817, 1200, 1300, 1669
BspFNI CGCG 6 cut(s) 206, 208, 215, 763, 765, 1491
BspLI GGNNCC 8 cut(s) 625, 710, 844, 1318, 1326, 1463, 1557, 1581
BspMAI CTGCAG 2 cut(s) 34, 622
BspMI ACCTGC 2 cut(s) 325, 1567
BspQI GCTCTTC 2 cut(s) 1068, 1665
BspT104I TTCGAA 1 cut(s) 1171
BspT107I GGYRCC 2 cut(s) 708, 842
BsrDI GCAATG 3 cut(s) 281, 490, 1599
BsrI ACTGG 1 cut(s) 1240
BssECI CCNNGG 6 cut(s) 193, 599, 681, 846, 1320, 1465
BssHII GCGCGC 3 cut(s) 204, 761, 763
BssNAI GTATAC 1 cut(s) 1155
BssT1I CCWWGG 4 cut(s) 193, 846, 1320, 1465
Bst1107I GTATAC 1 cut(s) 1155
Bst2UI CCWGG 1 cut(s) 682
Bst4CI ACNGT 2 cut(s) 10, 988
Bst6I CTCTTC 3 cut(s) 630, 1068, 1665
BstAPI GCANNNNNTGC 3 cut(s) 10, 305, 857
BstBI TTCGAA 1 cut(s) 1171
BstC8I GCNNGC 8 cut(s) 55, 206, 351, 647, 749, 763, 765, 1359
BstDSI CCRYGG 2 cut(s) 846, 1320
BstF5I GGATG 3 cut(s) 169, 523, 792
BstFNI CGCG 6 cut(s) 206, 208, 215, 763, 765, 1491
BstHHI GCGC 8 cut(s) 206, 208, 349, 763, 765, 767, 1480, 1614
BstMAI GTCTC 2 cut(s) 680, 1540
BstNI CCWGG 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 680, 1723
BstSFI CTRYAG 2 cut(s) 30, 618
BstUI CGCG 6 cut(s) 206, 208, 215, 763, 765, 1491
BstV1I GCAGC 8 cut(s) 213, 604, 626, 833, 1055, 1112, 1429, 1601
BstV2I GAAGAC 2 cut(s) 704, 989
BstX2I RGATCY 7 cut(s) 176, 623, 1221, 1324, 1461, 1504, 1720
BstYI RGATCY 7 cut(s) 176, 623, 1221, 1324, 1461, 1504, 1720
BstZ17I GTATAC 1 cut(s) 1155
BsuRI GGCC 1 cut(s) 274
BtgI CCRYGG 2 cut(s) 846, 1320
BtsCI GGATG 3 cut(s) 169, 523, 792
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 3 cut(s) 39, 1195, 1711
BtuMI TCGCGA 2 cut(s) 215, 1491
BveI ACCTGC 2 cut(s) 325, 1567
Cac8I GCNNGC 8 cut(s) 55, 206, 351, 647, 749, 763, 765, 1359
CaiI CAGNNNCTG 2 cut(s) 931, 1445
CfoI GCGC 8 cut(s) 206, 208, 349, 763, 765, 767, 1480, 1614
Cfr13I GGNCC 2 cut(s) 243, 678
CseI GACGC 1 cut(s) 709
Csp6I GTAC 2 cut(s) 318, 1378
CviAII CATG 8 cut(s) 109, 401, 461, 847, 1097, 1141, 1321, 1510
CviQI GTAC 2 cut(s) 318, 1378
DraI TTTAAA 1 cut(s) 1039
Eam1104I CTCTTC 3 cut(s) 630, 1068, 1665
EarI CTCTTC 3 cut(s) 630, 1068, 1665
Eco130I CCWWGG 4 cut(s) 193, 846, 1320, 1465
Eco47I GGWCC 2 cut(s) 243, 678
Eco57I CTGAAG 4 cut(s) 235, 834, 852, 1548
Eco88I CYCGRG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 678
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 1 cut(s) 680
EcoT14I CCWWGG 4 cut(s) 193, 846, 1320, 1465
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 193, 846, 1320, 1465
FaeI CATG 8 cut(s) 112, 404, 464, 850, 1100, 1144, 1324, 1513
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FatI CATG 8 cut(s) 108, 400, 460, 846, 1096, 1140, 1320, 1509
FauI CCCGC 1 cut(s) 756
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 1 cut(s) 1154
Fnu4HI GCNGC 8 cut(s) 202, 615, 618, 822, 1044, 1101, 1443, 1615
FokI GGATG 3 cut(s) 176, 530, 779
Fsp4HI GCNGC 8 cut(s) 202, 615, 618, 822, 1044, 1101, 1443, 1615
FspBI CTAG 1 cut(s) 1049
GlaI GCGC 8 cut(s) 205, 207, 348, 762, 764, 766, 1479, 1613
GluI GCNGC 8 cut(s) 202, 615, 618, 822, 1044, 1101, 1443, 1615
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 1 cut(s) 274
HapII CCGG 2 cut(s) 241, 1725
HgaI GACGC 1 cut(s) 709
HhaI GCGC 8 cut(s) 206, 208, 349, 763, 765, 767, 1480, 1614
Hin1II CATG 8 cut(s) 112, 404, 464, 850, 1100, 1144, 1324, 1513
Hin6I GCGC 8 cut(s) 204, 206, 347, 761, 763, 765, 1478, 1612
HinP1I GCGC 8 cut(s) 204, 206, 347, 761, 763, 765, 1478, 1612
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 2 cut(s) 473, 1748
HinfI GANTC 5 cut(s) 362, 566, 999, 1182, 1286
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 2 cut(s) 241, 1725
HphI GGTGA 1 cut(s) 84
Hpy166II GTNNAC 2 cut(s) 579, 1155
Hpy188I TCNGA 6 cut(s) 86, 556, 1309, 1393, 1567, 1758
Hpy188III TCNNGA 8 cut(s) 214, 434, 563, 587, 909, 1459, 1490, 1627
Hpy8I GTNNAC 2 cut(s) 579, 1155
Hpy99I CGWCG 2 cut(s) 126, 129
HpyAV CCTTC 6 cut(s) 105, 154, 259, 827, 986, 1753
HpyCH4III ACNGT 2 cut(s) 10, 988
HpyCH4IV ACGT 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 8 cut(s) 112, 404, 464, 850, 1100, 1144, 1324, 1513
HspAI GCGC 8 cut(s) 204, 206, 347, 761, 763, 765, 1478, 1612
Ksp22I TGATCA 1 cut(s) 970
KspAI GTTAAC 1 cut(s) 579
LguI GCTCTTC 2 cut(s) 1068, 1665
LmnI GCTCC 1 cut(s) 1633
Lsp1109I GCAGC 8 cut(s) 213, 604, 626, 833, 1055, 1112, 1429, 1601
LweI GCATC 3 cut(s) 295, 860, 1702
MaeI CTAG 1 cut(s) 1049
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 2 cut(s) 1190, 1231
MfeI CAATTG 2 cut(s) 855, 1065
MflI RGATCY 7 cut(s) 176, 623, 1221, 1324, 1461, 1504, 1720
MluCI AATT 8 cut(s) 855, 975, 1065, 1336, 1482, 1521, 1533, 1669
MlyI GAGTC 1 cut(s) 1176
MmeI TCCRAC 2 cut(s) 744, 1159
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 1659
MseI TTAA 8 cut(s) 71, 269, 512, 578, 770, 1038, 1226, 1599
MspA1I CMGCKG 4 cut(s) 617, 824, 1442, 1543
MspI CCGG 2 cut(s) 241, 1725
MspR9I CCNGG 2 cut(s) 682, 1725
MunI CAATTG 2 cut(s) 855, 1065
Mva1269I GAATGC 3 cut(s) 443, 1179, 1359
MvaI CCWGG 1 cut(s) 682
MvnI CGCG 6 cut(s) 206, 208, 215, 763, 765, 1491
NciI CCSGG 1 cut(s) 1725
NcoI CCATGG 2 cut(s) 846, 1320
NlaIII CATG 8 cut(s) 112, 404, 464, 850, 1100, 1144, 1324, 1513
NlaIV GGNNCC 8 cut(s) 625, 710, 844, 1318, 1326, 1463, 1557, 1581
NmeAIII GCCGAG 2 cut(s) 355, 624
NmuCI GTSAC 1 cut(s) 1190
NruI TCGCGA 2 cut(s) 215, 1491
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 1171
PauI GCGCGC 3 cut(s) 204, 761, 763
PciSI GCTCTTC 2 cut(s) 1068, 1665
PcsI WCGNNNNNNNCGW 1 cut(s) 1177
PctI GAATGC 3 cut(s) 443, 1179, 1359
PdmI GAANNNNTTC 1 cut(s) 1659
PfeI GAWTC 4 cut(s) 362, 566, 999, 1286
PflMI CCANNNNNTGG 1 cut(s) 1141
PfoI TCCNGGA 1 cut(s) 1723
PkrI GCNGC 8 cut(s) 203, 616, 619, 823, 1045, 1102, 1444, 1616
PleI GAGTC 1 cut(s) 1176
PpsI GAGTC 1 cut(s) 1176
PpuMI RGGWCCY 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 680
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 680
PspN4I GGNNCC 8 cut(s) 625, 710, 844, 1318, 1326, 1463, 1557, 1581
PspPI GGNCC 2 cut(s) 243, 678
PspPPI RGGWCCY 1 cut(s) 678
PstI CTGCAG 2 cut(s) 34, 622
PstNI CAGNNNCTG 2 cut(s) 931, 1445
PsuI RGATCY 7 cut(s) 176, 623, 1221, 1324, 1461, 1504, 1720
PteI GCGCGC 3 cut(s) 204, 761, 763
PvuII CAGCTG 3 cut(s) 617, 824, 1442
RruI TCGCGA 2 cut(s) 215, 1491
RsaI GTAC 2 cut(s) 319, 1379
RsaNI GTAC 2 cut(s) 318, 1378
SapI GCTCTTC 2 cut(s) 1068, 1665
SaqAI TTAA 8 cut(s) 71, 269, 512, 578, 770, 1038, 1226, 1599
SatI GCNGC 8 cut(s) 202, 615, 618, 822, 1044, 1101, 1443, 1615
Sau96I GGNCC 2 cut(s) 243, 678
SchI GAGTC 1 cut(s) 1176
ScrFI CCNGG 2 cut(s) 682, 1725
SfaNI GCATC 3 cut(s) 295, 860, 1702
SfcI CTRYAG 2 cut(s) 30, 618
SfuI TTCGAA 1 cut(s) 1171
SinI GGWCC 2 cut(s) 243, 678
SmlI CTYRAG 2 cut(s) 1296, 1550
SmoI CTYRAG 2 cut(s) 1296, 1550
Sse9I AATT 8 cut(s) 855, 975, 1065, 1336, 1482, 1521, 1533, 1669
SsiI CCGC 3 cut(s) 208, 749, 1543
SspI AATATT 2 cut(s) 659, 1131
SspMI CTAG 1 cut(s) 1049
StyD4I CCNGG 2 cut(s) 680, 1723
StyI CCWWGG 4 cut(s) 193, 846, 1320, 1465
TaaI ACNGT 2 cut(s) 10, 988
TaiI ACGT 1 cut(s) 124
TaqI TCGA 7 cut(s) 155, 522, 908, 1060, 1171, 1180, 1281
TasI AATT 8 cut(s) 855, 975, 1065, 1336, 1482, 1521, 1533, 1669
TatI WGTACW 1 cut(s) 317
TfiI GAWTC 4 cut(s) 362, 566, 999, 1286
Tru1I TTAA 8 cut(s) 71, 269, 512, 578, 770, 1038, 1226, 1599
Tru9I TTAA 8 cut(s) 71, 269, 512, 578, 770, 1038, 1226, 1599
TscAI CASTG 3 cut(s) 39, 1195, 1711
TseFI GTSAC 1 cut(s) 1190
TseI GCWGC 8 cut(s) 201, 614, 617, 821, 1043, 1100, 1442, 1614
Tsp45I GTSAC 1 cut(s) 1190
TspDTI ATGAA 7 cut(s) 12, 125, 417, 1140, 1218, 1299, 1349
TspGWI ACGGA 1 cut(s) 1517
TspRI CASTG 3 cut(s) 39, 1195, 1711
Van91I CCANNNNNTGG 1 cut(s) 1141
VpaK11BI GGWCC 2 cut(s) 243, 678
XapI RAATTY 1 cut(s) 1336
XmiI GTMKAC 1 cut(s) 1154
XmnI GAANNNNTTC 1 cut(s) 1659
XspI CTAG 1 cut(s) 1049
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.